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The map label for this gene is rlmB [H]

Identifier: 229587012

GI number: 229587012

Start: 946274

End: 946996

Strand: Direct

Name: rlmB [H]

Synonym: RAF_ORF0915

Alternate gene names: 229587012

Gene position: 946274-946996 (Clockwise)

Preceding gene: 229586981

Following gene: 229587013

Centisome position: 74.01

GC content: 29.46

Gene sequence:

>723_bases
ATGCAAAATAAAAAACTTGATTCTAAACATTGTTATTATATGTATGGGAAACATCCTGTATTTTCTGCTCTAAATAATCC
AAAACGTCAAATTGACAATATTTTATGCACTAGGGAAATTTTTGAGACAAATAAAAAATTAATAGGTACTAGAGCTTATG
AAATTGTTAATAACGATATTTTATCTAAATTACTGGAAAACCAAACACATCAAGGAATAGCTGCAAAAATTAGACCAATT
TTTTCTTACAATTTAGAGGACATAGATATAACAAATCCAAAATGTAAAATTGCGATTCTTGATCAAATAACAGACACACA
AAATATTGGCGCAATTATTCGTAGTGCCGCTGCTTTTGATATAAATGCTATAATATTACCTCAAGATAATTCTCCAAATG
AAAGCGGCAGCATTGCTAAAGCGGCTTGCGGTACTTTAGAATTAATACCTATAATTAAAGTTATTAACTTACGCTCATGT
ATGAATTATCTTAAAAAACACGGCTTTTGGATTATAGGTCTAACAGGTTCTGCAAATGACTACTTTACCGATAAATTAAT
TTCCGACAAAATAGTATTGGTATTTGGCTCAGAAGATAAAGGTATACGAAAATTAGTTGCAGAAACATGTGATTATTTAG
CCAAAATCCCTATTTCTCAGAGAGTAGAAAGTCTTAATGTATCAAATGCCGCTTCTATAATTTTTCATTCATTACATAAA
TAA

Upstream 100 bases:

>100_bases
TCAAATAAACAAATAGCAGCTTGCACATTTATTTAGTTGTAACTTTCTATAATAAATAGTAGTTTCTGTCAAGAACAAAT
TACTGACGGTTTTCAATAAG

Downstream 100 bases:

>100_bases
TATTTTTAATAAATCAAATATTGTTTTTTATTTACTTCGATATAAATATTTAGTCTACTAAAATTAAAGTCATAAACTTA
TTAATTTGAGAGAAAAGTAA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MQNKKLDSKHCYYMYGKHPVFSALNNPKRQIDNILCTREIFETNKKLIGTRAYEIVNNDILSKLLENQTHQGIAAKIRPI
FSYNLEDIDITNPKCKIAILDQITDTQNIGAIIRSAAAFDINAIILPQDNSPNESGSIAKAACGTLELIPIIKVINLRSC
MNYLKKHGFWIIGLTGSANDYFTDKLISDKIVLVFGSEDKGIRKLVAETCDYLAKIPISQRVESLNVSNAASIIFHSLHK

Sequences:

>Translated_240_residues
MQNKKLDSKHCYYMYGKHPVFSALNNPKRQIDNILCTREIFETNKKLIGTRAYEIVNNDILSKLLENQTHQGIAAKIRPI
FSYNLEDIDITNPKCKIAILDQITDTQNIGAIIRSAAAFDINAIILPQDNSPNESGSIAKAACGTLELIPIIKVINLRSC
MNYLKKHGFWIIGLTGSANDYFTDKLISDKIVLVFGSEDKGIRKLVAETCDYLAKIPISQRVESLNVSNAASIIFHSLHK
>Mature_240_residues
MQNKKLDSKHCYYMYGKHPVFSALNNPKRQIDNILCTREIFETNKKLIGTRAYEIVNNDILSKLLENQTHQGIAAKIRPI
FSYNLEDIDITNPKCKIAILDQITDTQNIGAIIRSAAAFDINAIILPQDNSPNESGSIAKAACGTLELIPIIKVINLRSC
MNYLKKHGFWIIGLTGSANDYFTDKLISDKIVLVFGSEDKGIRKLVAETCDYLAKIPISQRVESLNVSNAASIIFHSLHK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=257, Percent_Identity=24.124513618677, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1790623, Length=191, Percent_Identity=35.0785340314136, Blast_Score=131, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17555908, Length=252, Percent_Identity=25.3968253968254, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=280, Percent_Identity=26.0714285714286, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26852; Mature: 26852

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKKLDSKHCYYMYGKHPVFSALNNPKRQIDNILCTREIFETNKKLIGTRAYEIVNNDI
CCCCCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LSKLLENQTHQGIAAKIRPIFSYNLEDIDITNPKCKIAILDQITDTQNIGAIIRSAAAFD
HHHHHHCCCCCCHHHHHHHHEECCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHEE
INAIILPQDNSPNESGSIAKAACGTLELIPIIKVINLRSCMNYLKKHGFWIIGLTGSAND
EEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
YFTDKLISDKIVLVFGSEDKGIRKLVAETCDYLAKIPISQRVESLNVSNAASIIFHSLHK
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQNKKLDSKHCYYMYGKHPVFSALNNPKRQIDNILCTREIFETNKKLIGTRAYEIVNNDI
CCCCCCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LSKLLENQTHQGIAAKIRPIFSYNLEDIDITNPKCKIAILDQITDTQNIGAIIRSAAAFD
HHHHHHCCCCCCHHHHHHHHEECCCCEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHEE
INAIILPQDNSPNESGSIAKAACGTLELIPIIKVINLRSCMNYLKKHGFWIIGLTGSAND
EEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCC
YFTDKLISDKIVLVFGSEDKGIRKLVAETCDYLAKIPISQRVESLNVSNAASIIFHSLHK
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]