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The map label for this gene is rluA2 [C]

Identifier: 229586865

GI number: 229586865

Start: 769085

End: 769777

Strand: Direct

Name: rluA2 [C]

Synonym: RAF_ORF0736

Alternate gene names: 229586865

Gene position: 769085-769777 (Clockwise)

Preceding gene: 229586863

Following gene: 229586866

Centisome position: 60.15

GC content: 31.17

Gene sequence:

>693_bases
ATGCATAGATTAGCTAAAATAATTAGTAATGCAGGAGTTTGTTCTAGGCGTGATGCTGAAAAGCTGATAGTTGAAGGACA
GGTAAAAGTAGACGGCGTTACAATTTTATCACCAACTACAAATGTTGATATTAGTAATCAAATTGAAGTATCAGGCACAT
TAATCAATAATATACACAAACCAAGGCTTTGGATTTATTACAAACCTGTTGGTTTAATTACCACTCATAAAGATCCTTTA
TCTCGTAAAACGGTGTTTGAACAGCTAACCGGTTTACCTCGTGTTATTTCAATAGGTAGGCTGGATTTAAATAGCGAAGG
TTTATTATTACTAACTAATAGCGGTGATTTAGCTCGTCAGTTTGAGCTCCCCTCAAGTAGGTTAAAGCGAGTATATCATG
TTAGGGCGTATGGGAAGTCTGATATTCTACTAAAAAGCAATTATAAAAATTTAGAAATTGACGGGATATTTTATAACCCT
CATTCGATAAAATTACTTAAGCAAAATAAAACTAATTGTTGGTTTGAAGTAGTTTTATTTGAAGGAAAAAATCGTGAAAT
TAGAAGAATATTCGAGTATTGTGGACTAAAAGTTAACAAATTAATTAGGATTCAATATGGTAGTTTTACAATAGATAATC
TAAAACCCGGAGATTATAAAGAAATAAATAATAAAATACTGGAAAATCATTAG

Upstream 100 bases:

>100_bases
ATATAGAAATACGTGAAGATGCGAGTACCGAATCGACGTATAAATTATCTCTAGAAACGACGCTTGGGAAGAGGTCTATT
GAAAATTACTATATTTAAAA

Downstream 100 bases:

>100_bases
TAATGTGTTATTATTAATACATTTTAACTAATCATATAGAATACATATGACAAATCTTAATATATATAAATGTACAGAAA
AAGACCTAAACGATTACCTT

Product: Ribosomal large subunit pseudouridine synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MHRLAKIISNAGVCSRRDAEKLIVEGQVKVDGVTILSPTTNVDISNQIEVSGTLINNIHKPRLWIYYKPVGLITTHKDPL
SRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQFELPSSRLKRVYHVRAYGKSDILLKSNYKNLEIDGIFYNP
HSIKLLKQNKTNCWFEVVLFEGKNREIRRIFEYCGLKVNKLIRIQYGSFTIDNLKPGDYKEINNKILENH

Sequences:

>Translated_230_residues
MHRLAKIISNAGVCSRRDAEKLIVEGQVKVDGVTILSPTTNVDISNQIEVSGTLINNIHKPRLWIYYKPVGLITTHKDPL
SRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQFELPSSRLKRVYHVRAYGKSDILLKSNYKNLEIDGIFYNP
HSIKLLKQNKTNCWFEVVLFEGKNREIRRIFEYCGLKVNKLIRIQYGSFTIDNLKPGDYKEINNKILENH
>Mature_230_residues
MHRLAKIISNAGVCSRRDAEKLIVEGQVKVDGVTILSPTTNVDISNQIEVSGTLINNIHKPRLWIYYKPVGLITTHKDPL
SRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQFELPSSRLKRVYHVRAYGKSDILLKSNYKNLEIDGIFYNP
HSIKLLKQNKTNCWFEVVLFEGKNREIRRIFEYCGLKVNKLIRIQYGSFTIDNLKPGDYKEINNKILENH

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=35.9307359307359, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=28.755364806867, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=24.0174672489083, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26376; Mature: 26376

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRLAKIISNAGVCSRRDAEKLIVEGQVKVDGVTILSPTTNVDISNQIEVSGTLINNIHK
CCHHHHHHHCCCCCCCCCCCEEEEECEEEECCEEEECCCCCCCCCCEEEEEEEEECCCCC
PRLWIYYKPVGLITTHKDPLSRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQ
CEEEEEEECEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCEEE
FELPSSRLKRVYHVRAYGKSDILLKSNYKNLEIDGIFYNPHSIKLLKQNKTNCWFEVVLF
ECCCHHHHHHEEEEEECCCCCEEEECCCCEEEEEEEEECCCEEEEEECCCCCEEEEEEEE
EGKNREIRRIFEYCGLKVNKLIRIQYGSFTIDNLKPGDYKEINNKILENH
ECCCHHHHHHHHHCCCEEEEEEEEEECCEEECCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHRLAKIISNAGVCSRRDAEKLIVEGQVKVDGVTILSPTTNVDISNQIEVSGTLINNIHK
CCHHHHHHHCCCCCCCCCCCEEEEECEEEECCEEEECCCCCCCCCCEEEEEEEEECCCCC
PRLWIYYKPVGLITTHKDPLSRKTVFEQLTGLPRVISIGRLDLNSEGLLLLTNSGDLARQ
CEEEEEEECEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCEEE
FELPSSRLKRVYHVRAYGKSDILLKSNYKNLEIDGIFYNPHSIKLLKQNKTNCWFEVVLF
ECCCHHHHHHEEEEEECCCCCEEEECCCCEEEEEEEEECCCEEEEEECCCCCEEEEEEEE
EGKNREIRRIFEYCGLKVNKLIRIQYGSFTIDNLKPGDYKEINNKILENH
ECCCHHHHHHHHHCCCEEEEEEEEEECCEEECCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]