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The map label for this gene is dnaB [H]

Identifier: 229586863

GI number: 229586863

Start: 765733

End: 767217

Strand: Direct

Name: dnaB [H]

Synonym: RAF_ORF0734

Alternate gene names: 229586863

Gene position: 765733-767217 (Clockwise)

Preceding gene: 229586862

Following gene: 229586865

Centisome position: 59.89

GC content: 32.73

Gene sequence:

>1485_bases
ATGGCACGTAATAAAATAAATAACGAAACAAATATATTAGCTGATAACGAAGATAATTTACCTATACCAAGGGTTTTACC
TTCAAATGTTCAAGCAGAGCAAATGCTACTTGGGGCGATTTTAACCAATAACGAATTACTAAATTACGTATCGGAATTTT
TACGTGATGAACATTTTTTTGAACCTATTCATCAAAAAATCTATAAGGCAATTGAGAAAATTACTGAAAAAGGGCTGACA
GCTACACCTATTACTTTACGTAGTATGTTAACTCAAGATGAACTATTTCAAGAAGTAGAAGGAGCGGAGTATTTAGCAAA
ATTGATAACTATGTCAATGATGGTAATAAATCCGCGTGATTATGGTAAAATAATATATGATTTAGCAATAAAGCGTAATT
TAATAAATATCGGTGAAGAAGTAGTAAATAATGCCTATAATTCTTCATTAGAAGTTGAAGCAAAGGAACAGATTGAACAT
GCCGAAGCTAAACTTTACGATTTAGCTAGTGAAGGCTTGAATGAAAAAAGTTTTACCAAAATCGGTATATCTATTTCAGA
ATCACTAGCTAGTATTAATAGGGCTATGAAAAATAATGATCATATAATCGGTATATCTACCGGTTTGATTGATCTAGATA
ATAAATTATGCGGCTTTCATAATTCCGATCTTATAATTCTTGCAGGACGTCCGTCTATGGGTAAAACGGCATTTGCTATA
AATCTTGCACTTAATGCCTGTAATAATATGCGTCTTAAAAATATTAGAGATAATCAAGAAATTCAGTCAGTAGGTTTTTT
CTCTTTAGAAATGTCCTCAGAACAGCTAACCACACGTCTACTTTCAATGTGTGCAGAAATTGATTCTACTTCTCTTCGTA
CGGGGATTCTAGGTGAAGAAAAATATAACCGTCTTCGCAAGGAAGCAAATACTTTATCGGAATTACAATTTTTTATCGAT
GATACCCCTGCTCTATCTATTTCCGCTATAAGAACAAGAGCTAGAAGAATGAAACGCAAGCATAATCTCGGTATATTATT
TATTGATTATTTACAATTAATTAGAGGAGTAAGTAAATCCGAAAATAGAGTTAGCGAGATTTCAGAAATAACCCAAGGTT
TAAAAGCAATAGCAAAAGAGTTAAATATTCCGGTTATTGCGTTATCTCAGCTTTCAAGAGCGGTAGAACTACGTGAGGAC
AAAAAACCTATGCTATCCGATCTTCGAGAATCGGGAACTATAGAACAGGATGCTGATATAGTAATGTTTATTTATCGTGA
AGAATATTATTTAACTAGAAAAGAACCGGCAGCAGGCGATGCTAAACATGCCGCATGGTTAGATAAACTCAACAAAGTAT
ATAATATTGCCGATATAATTGTTGCAAAACATCGTAACGGACCAGTTGGAAATGTTCCGATTTATTATGATAGTCAATTT
TCTAAATTTGGTAATTTGGAGACAAGAACTTTTAACTCTAATTAA

Upstream 100 bases:

>100_bases
GCTGCTTGCAATGACGACTCGGTATCCACGCAACAATACCTCGTGGGAATGACATAAAGGGCTTCTTTTTTCAAACCATA
TAATAATTTAACAAACAACA

Downstream 100 bases:

>100_bases
ATCAGAATTGTTTTACATCATTATTGCGTGAATACCAAATCGTCATTGCGAGCGAACGTTAGTGAGCGTGGCAATCTCAG
GAATTTTTCCTGAGATTGCT

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MARNKINNETNILADNEDNLPIPRVLPSNVQAEQMLLGAILTNNELLNYVSEFLRDEHFFEPIHQKIYKAIEKITEKGLT
ATPITLRSMLTQDELFQEVEGAEYLAKLITMSMMVINPRDYGKIIYDLAIKRNLINIGEEVVNNAYNSSLEVEAKEQIEH
AEAKLYDLASEGLNEKSFTKIGISISESLASINRAMKNNDHIIGISTGLIDLDNKLCGFHNSDLIILAGRPSMGKTAFAI
NLALNACNNMRLKNIRDNQEIQSVGFFSLEMSSEQLTTRLLSMCAEIDSTSLRTGILGEEKYNRLRKEANTLSELQFFID
DTPALSISAIRTRARRMKRKHNLGILFIDYLQLIRGVSKSENRVSEISEITQGLKAIAKELNIPVIALSQLSRAVELRED
KKPMLSDLRESGTIEQDADIVMFIYREEYYLTRKEPAAGDAKHAAWLDKLNKVYNIADIIVAKHRNGPVGNVPIYYDSQF
SKFGNLETRTFNSN

Sequences:

>Translated_494_residues
MARNKINNETNILADNEDNLPIPRVLPSNVQAEQMLLGAILTNNELLNYVSEFLRDEHFFEPIHQKIYKAIEKITEKGLT
ATPITLRSMLTQDELFQEVEGAEYLAKLITMSMMVINPRDYGKIIYDLAIKRNLINIGEEVVNNAYNSSLEVEAKEQIEH
AEAKLYDLASEGLNEKSFTKIGISISESLASINRAMKNNDHIIGISTGLIDLDNKLCGFHNSDLIILAGRPSMGKTAFAI
NLALNACNNMRLKNIRDNQEIQSVGFFSLEMSSEQLTTRLLSMCAEIDSTSLRTGILGEEKYNRLRKEANTLSELQFFID
DTPALSISAIRTRARRMKRKHNLGILFIDYLQLIRGVSKSENRVSEISEITQGLKAIAKELNIPVIALSQLSRAVELRED
KKPMLSDLRESGTIEQDADIVMFIYREEYYLTRKEPAAGDAKHAAWLDKLNKVYNIADIIVAKHRNGPVGNVPIYYDSQF
SKFGNLETRTFNSN
>Mature_493_residues
ARNKINNETNILADNEDNLPIPRVLPSNVQAEQMLLGAILTNNELLNYVSEFLRDEHFFEPIHQKIYKAIEKITEKGLTA
TPITLRSMLTQDELFQEVEGAEYLAKLITMSMMVINPRDYGKIIYDLAIKRNLINIGEEVVNNAYNSSLEVEAKEQIEHA
EAKLYDLASEGLNEKSFTKIGISISESLASINRAMKNNDHIIGISTGLIDLDNKLCGFHNSDLIILAGRPSMGKTAFAIN
LALNACNNMRLKNIRDNQEIQSVGFFSLEMSSEQLTTRLLSMCAEIDSTSLRTGILGEEKYNRLRKEANTLSELQFFIDD
TPALSISAIRTRARRMKRKHNLGILFIDYLQLIRGVSKSENRVSEISEITQGLKAIAKELNIPVIALSQLSRAVELREDK
KPMLSDLRESGTIEQDADIVMFIYREEYYLTRKEPAAGDAKHAAWLDKLNKVYNIADIIVAKHRNGPVGNVPIYYDSQFS
KFGNLETRTFNSN

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=466, Percent_Identity=36.9098712446352, Blast_Score=297, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55800; Mature: 55668

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKINNETNILADNEDNLPIPRVLPSNVQAEQMLLGAILTNNELLNYVSEFLRDEHFF
CCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EPIHQKIYKAIEKITEKGLTATPITLRSMLTQDELFQEVEGAEYLAKLITMSMMVINPRD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCH
YGKIIYDLAIKRNLINIGEEVVNNAYNSSLEVEAKEQIEHAEAKLYDLASEGLNEKSFTK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHE
IGISISESLASINRAMKNNDHIIGISTGLIDLDNKLCGFHNSDLIILAGRPSMGKTAFAI
ECHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCEEEEECCCCCCCCEEEE
NLALNACNNMRLKNIRDNQEIQSVGFFSLEMSSEQLTTRLLSMCAEIDSTSLRTGILGEE
EEHHHHCCCCEECCCCCCHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHCCCCHH
KYNRLRKEANTLSELQFFIDDTPALSISAIRTRARRMKRKHNLGILFIDYLQLIRGVSKS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCC
ENRVSEISEITQGLKAIAKELNIPVIALSQLSRAVELREDKKPMLSDLRESGTIEQDADI
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCE
VMFIYREEYYLTRKEPAAGDAKHAAWLDKLNKVYNIADIIVAKHRNGPVGNVPIYYDSQF
EEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEECCCH
SKFGNLETRTFNSN
HHCCCCCCEEECCC
>Mature Secondary Structure 
ARNKINNETNILADNEDNLPIPRVLPSNVQAEQMLLGAILTNNELLNYVSEFLRDEHFF
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EPIHQKIYKAIEKITEKGLTATPITLRSMLTQDELFQEVEGAEYLAKLITMSMMVINPRD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCH
YGKIIYDLAIKRNLINIGEEVVNNAYNSSLEVEAKEQIEHAEAKLYDLASEGLNEKSFTK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHE
IGISISESLASINRAMKNNDHIIGISTGLIDLDNKLCGFHNSDLIILAGRPSMGKTAFAI
ECHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCCCEEEEECCCCCCCCEEEE
NLALNACNNMRLKNIRDNQEIQSVGFFSLEMSSEQLTTRLLSMCAEIDSTSLRTGILGEE
EEHHHHCCCCEECCCCCCHHHHHCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHCCCCHH
KYNRLRKEANTLSELQFFIDDTPALSISAIRTRARRMKRKHNLGILFIDYLQLIRGVSKS
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCC
ENRVSEISEITQGLKAIAKELNIPVIALSQLSRAVELREDKKPMLSDLRESGTIEQDADI
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCE
VMFIYREEYYLTRKEPAAGDAKHAAWLDKLNKVYNIADIIVAKHRNGPVGNVPIYYDSQF
EEEEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEECCCH
SKFGNLETRTFNSN
HHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA