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The map label for this gene is spl1 [H]

Identifier: 229586809

GI number: 229586809

Start: 706343

End: 707461

Strand: Direct

Name: spl1 [H]

Synonym: RAF_ORF0675

Alternate gene names: 229586809

Gene position: 706343-707461 (Clockwise)

Preceding gene: 229586808

Following gene: 229586810

Centisome position: 55.25

GC content: 32.8

Gene sequence:

>1119_bases
ATGATATATTTAGACCATAATGCTACTACTTTTATCGACCCTAGAGTTAAGGAATTCATAATAAGTTTAATGGACAAGGA
GCTTAACCCTTCATCAGCACATAGCTCAGGTAGATTTGCTAAGAATCTCATAGAAACGGTACGCGCACAAATAGCGACGG
CTCTTGGTATAACCTTATCATCTAGAGAATATGATATTACTTTTACTTCATCAGGAACTGAAGGTAATAATTTAATAATG
AAAAATTTTTATGACGGCGATATTTTTATTTCAGCTATTGAACATTTATCGATCTATAACCATATAAACTACGCTCCAAA
TATTAAAGTTATAAGTGTTAATACGCAAGGGCTAGTTGATTTAGAACATTTAGAAGAGTTGTTAGCTCAAAGTAATACTA
GTAAAAAACTAGTTTCCATAATAATGGCTAATAATGAAAGCGGAGTTTTACAAGATATAGCTGAGATAGGTAAAATAACT
AAAAAATATGAGGCAAAATTTCATAGCGATTTAGTGCAAGGTTTTGGCAGAATACCAATAAATATCAAAGCATTAGGATT
AGATTTTGCTACAATTTCAGGGCATAAAATAGGAGGGGGGCAGGGTGGTGCTGCTTTAATTTCTAGCTCTAATTTTCAAA
TTACTCCAATGATTATAGGAGGAGGGCAGGAAAAATCAGTACGCTCAGGCACTGAAAATGTTTTAGCAATTGCAGGATTT
GGTTTAGCTTCTGCATTGAGGACAGATAATATCTCAGAAAATTATATAAAAATCAAAAAGTTACAAGAAAATTTAGAGAA
AAAATTAAAAAAATATCCTAATGTAAATATTGTTAGTAATAACGTAGCTAGGTTGCCTAATACTACCTTAATTACTATAC
CAAATACGGATGCACAAGCGAAATTAATCGGGTTTGATTTACATAATATTTGCGTAAGTTCCGGTTCTGCTTGTTCATCG
GGAAAAATATCTAAATCGCATGTATTAACCAATATGGGTGTAGGGGAAGAAGAAGCAAAATCTTCGATTAGGATATCTTT
AAGCCATACTAATACGGTACGTGATATAGAGGCTTTTATAGAGGCTTTTGAGGAGATTTACGATGTCATTCCTAGCTAA

Upstream 100 bases:

>100_bases
AATGTAATTCGCATAAATTATGGCAAGGTCTTGGTAAGCATATTAGAGATTATTTTGAAAATATCTCAATTAAAGATGCT
TTAAACTCAAATTTAAATAT

Downstream 100 bases:

>100_bases
AAGCTACGTTGTTGCACGGCTCCGTTTATGTCATTCCCGCTTCCGCAAGAATGACATCGATGACTGTTTAAACTATACCA
AAACATGAATTAATTTAACC

Product: NifS-like protein

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIYLDHNATTFIDPRVKEFIISLMDKELNPSSAHSSGRFAKNLIETVRAQIATALGITLSSREYDITFTSSGTEGNNLIM
KNFYDGDIFISAIEHLSIYNHINYAPNIKVISVNTQGLVDLEHLEELLAQSNTSKKLVSIIMANNESGVLQDIAEIGKIT
KKYEAKFHSDLVQGFGRIPINIKALGLDFATISGHKIGGGQGGAALISSSNFQITPMIIGGGQEKSVRSGTENVLAIAGF
GLASALRTDNISENYIKIKKLQENLEKKLKKYPNVNIVSNNVARLPNTTLITIPNTDAQAKLIGFDLHNICVSSGSACSS
GKISKSHVLTNMGVGEEEAKSSIRISLSHTNTVRDIEAFIEAFEEIYDVIPS

Sequences:

>Translated_372_residues
MIYLDHNATTFIDPRVKEFIISLMDKELNPSSAHSSGRFAKNLIETVRAQIATALGITLSSREYDITFTSSGTEGNNLIM
KNFYDGDIFISAIEHLSIYNHINYAPNIKVISVNTQGLVDLEHLEELLAQSNTSKKLVSIIMANNESGVLQDIAEIGKIT
KKYEAKFHSDLVQGFGRIPINIKALGLDFATISGHKIGGGQGGAALISSSNFQITPMIIGGGQEKSVRSGTENVLAIAGF
GLASALRTDNISENYIKIKKLQENLEKKLKKYPNVNIVSNNVARLPNTTLITIPNTDAQAKLIGFDLHNICVSSGSACSS
GKISKSHVLTNMGVGEEEAKSSIRISLSHTNTVRDIEAFIEAFEEIYDVIPS
>Mature_372_residues
MIYLDHNATTFIDPRVKEFIISLMDKELNPSSAHSSGRFAKNLIETVRAQIATALGITLSSREYDITFTSSGTEGNNLIM
KNFYDGDIFISAIEHLSIYNHINYAPNIKVISVNTQGLVDLEHLEELLAQSNTSKKLVSIIMANNESGVLQDIAEIGKIT
KKYEAKFHSDLVQGFGRIPINIKALGLDFATISGHKIGGGQGGAALISSSNFQITPMIIGGGQEKSVRSGTENVLAIAGF
GLASALRTDNISENYIKIKKLQENLEKKLKKYPNVNIVSNNVARLPNTTLITIPNTDAQAKLIGFDLHNICVSSGSACSS
GKISKSHVLTNMGVGEEEAKSSIRISLSHTNTVRDIEAFIEAFEEIYDVIPS

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=375, Percent_Identity=31.4666666666667, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=29.951690821256, Blast_Score=164, Evalue=8e-41,
Organism=Escherichia coli, GI48994898, Length=376, Percent_Identity=32.9787234042553, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787970, Length=378, Percent_Identity=24.8677248677249, Blast_Score=68, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=404, Percent_Identity=31.1881188118812, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=28.9634146341463, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319831, Length=378, Percent_Identity=31.4814814814815, Blast_Score=175, Evalue=1e-44,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=31.8059299191375, Blast_Score=168, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40402; Mature: 40402

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLDHNATTFIDPRVKEFIISLMDKELNPSSAHSSGRFAKNLIETVRAQIATALGITLS
CEEECCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEE
SREYDITFTSSGTEGNNLIMKNFYDGDIFISAIEHLSIYNHINYAPNIKVISVNTQGLVD
CCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEE
LEHLEELLAQSNTSKKLVSIIMANNESGVLQDIAEIGKITKKYEAKFHSDLVQGFGRIPI
HHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
NIKALGLDFATISGHKIGGGQGGAALISSSNFQITPMIIGGGQEKSVRSGTENVLAIAGF
EEEEEEEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCHHHCCCCCEEEEECC
GLASALRTDNISENYIKIKKLQENLEKKLKKYPNVNIVSNNVARLPNTTLITIPNTDAQA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEECCCCCCE
KLIGFDLHNICVSSGSACSSGKISKSHVLTNMGVGEEEAKSSIRISLSHTNTVRDIEAFI
EEEEEEHHHHHHCCCCCCCCCCCCHHHEEECCCCCHHHCCCEEEEEEECCCHHHHHHHHH
EAFEEIYDVIPS
HHHHHHHHHCCC
>Mature Secondary Structure
MIYLDHNATTFIDPRVKEFIISLMDKELNPSSAHSSGRFAKNLIETVRAQIATALGITLS
CEEECCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEE
SREYDITFTSSGTEGNNLIMKNFYDGDIFISAIEHLSIYNHINYAPNIKVISVNTQGLVD
CCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEE
LEHLEELLAQSNTSKKLVSIIMANNESGVLQDIAEIGKITKKYEAKFHSDLVQGFGRIPI
HHHHHHHHHCCCCCHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
NIKALGLDFATISGHKIGGGQGGAALISSSNFQITPMIIGGGQEKSVRSGTENVLAIAGF
EEEEEEEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCHHHCCCCCEEEEECC
GLASALRTDNISENYIKIKKLQENLEKKLKKYPNVNIVSNNVARLPNTTLITIPNTDAQA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCCCCEEEEECCCCCCE
KLIGFDLHNICVSSGSACSSGKISKSHVLTNMGVGEEEAKSSIRISLSHTNTVRDIEAFI
EEEEEEHHHHHHCCCCCCCCCCCCHHHEEECCCCCHHHCCCEEEEEEECCCHHHHHHHHH
EAFEEIYDVIPS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]