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The map label for this gene is iscS

Identifier: 229586810

GI number: 229586810

Start: 707564

End: 708796

Strand: Direct

Name: iscS

Synonym: RAF_ORF0676

Alternate gene names: 229586810

Gene position: 707564-708796 (Clockwise)

Preceding gene: 229586809

Following gene: 229586811

Centisome position: 55.34

GC content: 37.55

Gene sequence:

>1233_bases
ATGAACCCACAATTAAACAATTTAACCTTACCGATATATATGGATTATCAGGCGACGACTCCAATAGATCCAAGAGTAAT
GGAGGCAATGTTGCCTTATTTTACTACCAAGTTCGGCAATCCTCATTCACGCAGCCATTCTTTCGGTTGGGAGGCAGAAA
ACGCCGTTGAAGAAGCAAGGAGTATGGTAGCAAAGTTAATAGGAGCGGATACTAAAGAAATTATTTTCACTTCCGGTGCA
ACTGAGTCTAACAATCTTGCAATAAAGGGAATAGCAAAATTTTACAGCAATAAAAAGAATCATATTATTACCGTAGTCAG
TGAACATAAATGCGTACTTGATGCTTGCAGGCATTTAGAGCAAGAAGGTATAAAAATCACATACTTACCGATTAAGCCAA
ATGGAATAATCGATTTAGAAACTCTAAAAAATGCCATTACCGATCAGACTATGTTAGTTTCAGTTATGGTGGTTAATAAT
GAAATAGGTGTTGTTCAGCCTTTAAAGGAAATCGGGCAAATTTGCCGTGAAAAGGGCGTTTTTTTTCATTCCGATATTGC
TCAAGGTTTTGGTAAAATTCCAATTGATGTTAACGCATTTAATATTGATCTTGCCAGTATCTCAGGGCATAAAATTTACG
GTCCGAAAGGAATAGGTGCATTATATGTAAGGAAAAAACCTCGTGTGCGTGTTACACCGCTCATAAACGGTGGCGGACAG
GAGAGAGGTATACGTTCAGGTACGCTACCGACTCCTTTAATCGTAGGGCTTGGCATGGCTGCTGAAATAGCGTATAGTGA
GATGGAAAAAGATACTAAGCACATAAATTACTTATTCGACAGATTTTTAAATAATATACATAAGCGAATTTCAAAAGTTT
ATTTAAACGGCGATAAAAATCAAAGATATAAAGGTAATTTAAATTTAAGCTTTGCCGGAGTTGAAGGAGAATCAATGATC
CTTGCCATTAAAGATTTAGCGGTTTCCTCCGGTTCTGCTTGTACTTCTGCCTCTTTAGAACCGTCATATGTTTTACGTTC
TATGGGAATCGGTGAAGAGCTTGCCCATACTGCAATTAGGTTCGGCATAGGTAGATTTACTACCGAGCAGGAAGTTGACT
ACGCAGTAAATTTAATATGCTCAAAAATCGATAAACTAAGGGAATTAAGCCCTCTTTGGGAAATGATGCAAGAGGGAATT
GATTTGAAAAAGATTAAATGGGCTGTACATTGA

Upstream 100 bases:

>100_bases
GCTACGTTGTTGCACGGCTCCGTTTATGTCATTCCCGCTTCCGCAAGAATGACATCGATGACTGTTTAAACTATACCAAA
ACATGAATTAATTTAACCAT

Downstream 100 bases:

>100_bases
GAATATCGGCATTGCGAGCAGCTGTAGGCTGCGTGGCAATCTTATCAAATATCCTGAGATTGCTTCGTCAAAACTTGCAG
TTTTTCCTCGCAATGACGGC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGADTKEIIFTSGA
TESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNN
EIGVVQPLKEIGQICREKGVFFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
ERGIRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHINYLFDRFLNNIHKRISKVYLNGDKNQRYKGNLNLSFAGVEGESMI
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGI
DLKKIKWAVH

Sequences:

>Translated_410_residues
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGADTKEIIFTSGA
TESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNN
EIGVVQPLKEIGQICREKGVFFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
ERGIRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHINYLFDRFLNNIHKRISKVYLNGDKNQRYKGNLNLSFAGVEGESMI
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGI
DLKKIKWAVH
>Mature_410_residues
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEARSMVAKLIGADTKEIIFTSGA
TESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLEQEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNN
EIGVVQPLKEIGQICREKGVFFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
ERGIRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHINYLFDRFLNNIHKRISKVYLNGDKNQRYKGNLNLSFAGVEGESMI
LAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIRFGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGI
DLKKIKWAVH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=408, Percent_Identity=63.4803921568627, Blast_Score=572, Evalue=1e-163,
Organism=Homo sapiens, GI156713448, Length=416, Percent_Identity=35.3365384615385, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI48994898, Length=405, Percent_Identity=59.5061728395062, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1789175, Length=399, Percent_Identity=26.3157894736842, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787970, Length=409, Percent_Identity=24.9388753056235, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.5960099750624, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=34.5454545454545, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319831, Length=402, Percent_Identity=59.452736318408, Blast_Score=519, Evalue=1e-148,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=64.5, Blast_Score=578, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_RICAE (C3PNQ8)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845311.1
- ProteinModelPortal:   C3PNQ8
- GeneID:   7815167
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0676
- OMA:   SYVLRSM
- ProtClustDB:   PRK14012
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 45470; Mature: 45470

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEAR
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
SMVAKLIGADTKEIIFTSGATESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLE
HHHHHHHCCCCHHEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
QEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNNEIGVVQPLKEIGQICREKGV
HCCCEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
FFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
EEECHHHHCCCCCCEEEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEECCCCC
ERGIRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHINYLFDRFLNNIHKRISKVYLNGDKN
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
QRYKGNLNLSFAGVEGESMILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIR
CCEECCEEEEEECCCCCEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
FGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGIDLKKIKWAVH
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC
>Mature Secondary Structure
MNPQLNNLTLPIYMDYQATTPIDPRVMEAMLPYFTTKFGNPHSRSHSFGWEAENAVEEAR
CCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
SMVAKLIGADTKEIIFTSGATESNNLAIKGIAKFYSNKKNHIITVVSEHKCVLDACRHLE
HHHHHHHCCCCHHEEEECCCCCCCCEEEEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
QEGIKITYLPIKPNGIIDLETLKNAITDQTMLVSVMVVNNEIGVVQPLKEIGQICREKGV
HCCCEEEEEEECCCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCC
FFHSDIAQGFGKIPIDVNAFNIDLASISGHKIYGPKGIGALYVRKKPRVRVTPLINGGGQ
EEECHHHHCCCCCCEEEEEEEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEECCCCC
ERGIRSGTLPTPLIVGLGMAAEIAYSEMEKDTKHINYLFDRFLNNIHKRISKVYLNGDKN
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
QRYKGNLNLSFAGVEGESMILAIKDLAVSSGSACTSASLEPSYVLRSMGIGEELAHTAIR
CCEECCEEEEEECCCCCEEEEEEEEHHCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
FGIGRFTTEQEVDYAVNLICSKIDKLRELSPLWEMMQEGIDLKKIKWAVH
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA