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The map label for this gene is traD_F [H]

Identifier: 229586792

GI number: 229586792

Start: 685753

End: 686274

Strand: Direct

Name: traD_F [H]

Synonym: RAF_ORF0650

Alternate gene names: 229586792

Gene position: 685753-686274 (Clockwise)

Preceding gene: 229586791

Following gene: 229586795

Centisome position: 53.64

GC content: 36.21

Gene sequence:

>522_bases
ATGCTACGACCACTTATTACTGCATGGATTAGCATAGCGATAAAAGCATTAATGAATAGGAATATCAATAATGTTCATAG
TAATATGTGGTTTGTGATTGATGAGATGCCAGCTTTACACAAGATATCTTCACTCCCTATAGCTTTAGCTGAAAGCAGGA
AATATGGTGGCTGCGTTGTTGCAGGTATGCAGAATATCCATCAATTAGAGGAAATATATGGTCATGCAGGAGCAGCTTCA
ATGCTTGATCTATTTGGTAGTAAATTTATTTTTCGGGTAAGTGACTGGCAAACAGCACATAAATCAGCTTTAATGCTTGG
TGAGCAAGAAATAATCGAGACTCAAGAGAATCTCTCTTATGGTGCTAACTCCATGCGTGATGGAGTAAATATGCATAGCT
TAGAGAAAAGGAAGTTATTAGTAATGCCATCTGAAATTATGAATCTACCAGATCTTACCTGTTATGTAAAACTTGCGGGT
AATTTCCCTATTACTAAATTACCGATGAATTTACAAACTTGA

Upstream 100 bases:

>100_bases
TATAAAATATTATAATAATGCAGGTAGTTTTAGTATTAGAAAGTGGTTTACTGCGAACAAGGGTTGGTTATTTATCACAG
CAGCTCCTAACCCAAGAGTA

Downstream 100 bases:

>100_bases
TTTAAATCTAAAAAGATAAAACCATTTTAATGGCATTTTTAAAAGATTTTAATTGTTTGATAGGGGGGGATTTACCTTAT
AAAGAAAAATCCTATTATAA

Product: Putative conjugative transfer protein TraD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MLRPLITAWISIAIKALMNRNINNVHSNMWFVIDEMPALHKISSLPIALAESRKYGGCVVAGMQNIHQLEEIYGHAGAAS
MLDLFGSKFIFRVSDWQTAHKSALMLGEQEIIETQENLSYGANSMRDGVNMHSLEKRKLLVMPSEIMNLPDLTCYVKLAG
NFPITKLPMNLQT

Sequences:

>Translated_173_residues
MLRPLITAWISIAIKALMNRNINNVHSNMWFVIDEMPALHKISSLPIALAESRKYGGCVVAGMQNIHQLEEIYGHAGAAS
MLDLFGSKFIFRVSDWQTAHKSALMLGEQEIIETQENLSYGANSMRDGVNMHSLEKRKLLVMPSEIMNLPDLTCYVKLAG
NFPITKLPMNLQT
>Mature_173_residues
MLRPLITAWISIAIKALMNRNINNVHSNMWFVIDEMPALHKISSLPIALAESRKYGGCVVAGMQNIHQLEEIYGHAGAAS
MLDLFGSKFIFRVSDWQTAHKSALMLGEQEIIETQENLSYGANSMRDGVNMHSLEKRKLLVMPSEIMNLPDLTCYVKLAG
NFPITKLPMNLQT

Specific function: TraD is a cell envelope protein. The function of traD is unknown but it is essential for DNA transfer. It may form or may be part of a non-specific pore for DNA export, or it may directly energize DNA transport [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To traD of plasmid IncFII R100 [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014128
- InterPro:   IPR019476
- InterPro:   IPR022585 [H]

Pfam domain/function: PF12615 TraD_N; PF10412 TrwB_AAD_bind [H]

EC number: NA

Molecular weight: Translated: 19402; Mature: 19402

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
6.9 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
6.9 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPLITAWISIAIKALMNRNINNVHSNMWFVIDEMPALHKISSLPIALAESRKYGGCVV
CCHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCHHHHHHCCCEEEECCCCCCCEEE
AGMQNIHQLEEIYGHAGAASMLDLFGSKFIFRVSDWQTAHKSALMLGEQEIIETQENLSY
HHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHC
GANSMRDGVNMHSLEKRKLLVMPSEIMNLPDLTCYVKLAGNFPITKLPMNLQT
CCHHHHCCCCCHHHCCCEEEEECHHHCCCCCCEEEEEEECCCCEEECCCCCCC
>Mature Secondary Structure
MLRPLITAWISIAIKALMNRNINNVHSNMWFVIDEMPALHKISSLPIALAESRKYGGCVV
CCHHHHHHHHHHHHHHHHCCCCCHHHCCEEEEEECCCHHHHHHCCCEEEECCCCCCCEEE
AGMQNIHQLEEIYGHAGAASMLDLFGSKFIFRVSDWQTAHKSALMLGEQEIIETQENLSY
HHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHC
GANSMRDGVNMHSLEKRKLLVMPSEIMNLPDLTCYVKLAGNFPITKLPMNLQT
CCHHHHCCCCCHHHCCCEEEEECHHHCCCCCCEEEEEEECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2680768; 7915817; 10464182; 3323526; 2164585; 2163400; 1618779; 10498725 [H]