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The map label for this gene is dam2 [H]

Identifier: 229586795

GI number: 229586795

Start: 692424

End: 693236

Strand: Direct

Name: dam2 [H]

Synonym: RAF_ORF0659

Alternate gene names: 229586795

Gene position: 692424-693236 (Clockwise)

Preceding gene: 229586792

Following gene: 229586797

Centisome position: 54.16

GC content: 30.14

Gene sequence:

>813_bases
ATGTCAGTAAAGAACTTAGATAAACCACAACCATTCCTGCAATGGGTGGGTGGTAAAAGAAAAATCGTTGATCAATTAGT
TAAATTTATTCCTGAAAGACTAAACAACTACTATGAACCATTTCTTGGTGGTGGAGCTTTATTCTTTCATGTACGAGATA
AATTTAAACAATGCTATTTATCTGATATTAACCTTGAGCTAGTAACGTCCTATAACGCAGTTAAGACAAATCCTGATGCG
GTTAGTAAATTACTAGACTCACATAAAGCAAAGCACTCTAAAGAGCATTATTATCAAGTGAGAGCTAATAATGATAGTAG
TGATCCAATAAAAATTACTGCAAGATTTATTTATTTTAATAGATATTCATTTAAAGGTATTTACCAGATTAATATTGATG
GCAAACCAGCTCAAACTTTCTCTGGTAGGAATTATAGCAAATCTGATATTGCGTCTAGACTAAAGCAATGTAGTAGCTTA
CTTGCTGGCACATCCATTTGTGCTATAGATTTTTCTTTTATTGAACCACAACAAGATGATTTTGTTTATTTTGATCCTCC
TTATCATAAATCAGGTGAGAAATTCTATACTAGATTGCCATTTGATGAAAATGAGCAAACTAGACTTAGAGATTTTGCTC
TAGAGCTAAACAATAAAAATGTTAAAATAATGATTTCTAATAGCGACACTCCTTTTATTAGAAACTTATACAAGAATTTC
AATATTAAGATTATTAAAGTTAAATATTCTATGCCTGAGCACAATAAAATATCTGATGAAGTAGTTATTACAAATTATCA
ATTACCACAATAG

Upstream 100 bases:

>100_bases
ATAATAAATATTAAAATCCTTTTGAATATGCTGTTTTGCTCGCATTTAAGCCTCTTTTCTGATATAATCTTTAGTAGAAA
ATAAAGATAAATAACAAATT

Downstream 100 bases:

>100_bases
CTTAGTTATATTCAAACTTGATTAATTCAAAGCAATAATCTTGAGTCTTAAAAACTTCTTCTAGATCTTGCATATGACGA
TTTCTAACATAGTAATCATT

Product: Site-specific DNA adenine methylase

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.TpaI [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSVKNLDKPQPFLQWVGGKRKIVDQLVKFIPERLNNYYEPFLGGGALFFHVRDKFKQCYLSDINLELVTSYNAVKTNPDA
VSKLLDSHKAKHSKEHYYQVRANNDSSDPIKITARFIYFNRYSFKGIYQINIDGKPAQTFSGRNYSKSDIASRLKQCSSL
LAGTSICAIDFSFIEPQQDDFVYFDPPYHKSGEKFYTRLPFDENEQTRLRDFALELNNKNVKIMISNSDTPFIRNLYKNF
NIKIIKVKYSMPEHNKISDEVVITNYQLPQ

Sequences:

>Translated_270_residues
MSVKNLDKPQPFLQWVGGKRKIVDQLVKFIPERLNNYYEPFLGGGALFFHVRDKFKQCYLSDINLELVTSYNAVKTNPDA
VSKLLDSHKAKHSKEHYYQVRANNDSSDPIKITARFIYFNRYSFKGIYQINIDGKPAQTFSGRNYSKSDIASRLKQCSSL
LAGTSICAIDFSFIEPQQDDFVYFDPPYHKSGEKFYTRLPFDENEQTRLRDFALELNNKNVKIMISNSDTPFIRNLYKNF
NIKIIKVKYSMPEHNKISDEVVITNYQLPQ
>Mature_269_residues
SVKNLDKPQPFLQWVGGKRKIVDQLVKFIPERLNNYYEPFLGGGALFFHVRDKFKQCYLSDINLELVTSYNAVKTNPDAV
SKLLDSHKAKHSKEHYYQVRANNDSSDPIKITARFIYFNRYSFKGIYQINIDGKPAQTFSGRNYSKSDIASRLKQCSSLL
AGTSICAIDFSFIEPQQDDFVYFDPPYHKSGEKFYTRLPFDENEQTRLRDFALELNNKNVKIMISNSDTPFIRNLYKNFN
IKIIKVKYSMPEHNKISDEVVITNYQLPQ

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair, DNA replication, and gene expression [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=267, Percent_Identity=27.7153558052434, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31463; Mature: 31332

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKNLDKPQPFLQWVGGKRKIVDQLVKFIPERLNNYYEPFLGGGALFFHVRDKFKQCYL
CCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEHHHHHHHHH
SDINLELVTSYNAVKTNPDAVSKLLDSHKAKHSKEHYYQVRANNDSSDPIKITARFIYFN
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEEE
RYSFKGIYQINIDGKPAQTFSGRNYSKSDIASRLKQCSSLLAGTSICAIDFSFIEPQQDD
EECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCC
FVYFDPPYHKSGEKFYTRLPFDENEQTRLRDFALELNNKNVKIMISNSDTPFIRNLYKNF
EEEECCCCCCCCCEEEEECCCCCCCHHHHHHEEEEECCCEEEEEEECCCCHHHHHHHHCC
NIKIIKVKYSMPEHNKISDEVVITNYQLPQ
CEEEEEEEECCCCCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SVKNLDKPQPFLQWVGGKRKIVDQLVKFIPERLNNYYEPFLGGGALFFHVRDKFKQCYL
CCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEEHHHHHHHHH
SDINLELVTSYNAVKTNPDAVSKLLDSHKAKHSKEHYYQVRANNDSSDPIKITARFIYFN
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEEE
RYSFKGIYQINIDGKPAQTFSGRNYSKSDIASRLKQCSSLLAGTSICAIDFSFIEPQQDD
EECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCC
FVYFDPPYHKSGEKFYTRLPFDENEQTRLRDFALELNNKNVKIMISNSDTPFIRNLYKNF
EEEECCCCCCCCCEEEEECCCCCCCHHHHHHEEEEECCCEEEEEEECCCCHHHHHHHHCC
NIKIIKVKYSMPEHNKISDEVVITNYQLPQ
CEEEEEEEECCCCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9345771; 9665876 [H]