Click here to switch to the map view.

The map label for this gene is sppA2 [H]

Identifier: 229586788

GI number: 229586788

Start: 681981

End: 682895

Strand: Direct

Name: sppA2 [H]

Synonym: RAF_ORF0646

Alternate gene names: 229586788

Gene position: 681981-682895 (Clockwise)

Preceding gene: 229586787

Following gene: 229586789

Centisome position: 53.34

GC content: 33.88

Gene sequence:

>915_bases
ATGAGTATAACACCTGATTATTTAATTGAAAGAAGACAAATAAAATCTCGTTTGTTAATTTGGAAGCTAACAGCTATTAT
TTTAATTGCGATCGTATTCTTACTAGTCGGTAAAGATTTTGCTCCAAAAGAGGTGTTACCTATTAATAGTAATGAAGACT
ATATAGCTTCCGTATTAATAGACGAAATAATTCTTGAAGACGAAAAACGTGACAAAAAACTCAAAAAAATAATCGATGAT
TCTCATATTAAAGCGTTAATAGTTAACGTAAATTCTCCCGGCGGTACGGTAGTCGGCGCTGAAAAAATTTATAATATTCT
GCGTAAAATATCCGCAAAAAAGCCGGTAGTGATAGTTATGGGAACAATGGCTGCAAGCGGCGGTTATTTAATCTCTCTTG
GAGGTGATTATATTATCAGCCATAACGGAACTATTACGGGTTCAATCGGTGTAATACTACAAACAGCCGAGGTAACGGAG
CTTGCTCAAAAATTGGGGATTAAGTTTAATAATTTCAAATCGGGTGAATTAAAAGCTGTCCCAAATCCTACCGAAAAACT
GACGGAAGCAGTGCGGATAGCCATTATGGACAATATAGAAGATACTTATAATTTCTTCCTTGAACTTGTTTCAGAGAGGC
GTAATCTTCCTATAGAAGAAGTAAAAAAGCTCGCAGATGGACGAGTATATTCAGGTCGTCAGGCTTTAAAGTTAAAGCTT
GTGGATGCTATAGGCTCGGAAGATACTGCATTAAAATGGTTACAGGAAGTTCAGAAAATTAATGTTAATCTACTAGTTAA
AGATTATCAATTAAAACCAAAACCAAAATTAATGGACATAATACTTGAAGATTTTGATAGTATAGCACCTAGTTTTTTTA
AAAATAGTTTTAACGGAATCAAAGCGATTTTTTAA

Upstream 100 bases:

>100_bases
CGTTGTGCGTCGGCCTCACCTTATTATATTATAGGCTTCGCTCCTCACGCCTGTCACAGTATAGGGCTGCATTTAGTATT
CTAAAAAAGTATATAAGATC

Downstream 100 bases:

>100_bases
TAATGATTACTAAGAATTATTTAATAGATAAAATACATGATAAGCTGAATTATCTTTCTAAAGAAGATGTTAAAGATTCA
GTAGATTTAATTTTAGATTA

Product: Signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSITPDYLIERRQIKSRLLIWKLTAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLIDEIILEDEKRDKKLKKIIDD
SHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVMGTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTE
LAQKLGIKFNNFKSGELKAVPNPTEKLTEAVRIAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGIKAIF

Sequences:

>Translated_304_residues
MSITPDYLIERRQIKSRLLIWKLTAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLIDEIILEDEKRDKKLKKIIDD
SHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVMGTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTE
LAQKLGIKFNNFKSGELKAVPNPTEKLTEAVRIAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGIKAIF
>Mature_303_residues
SITPDYLIERRQIKSRLLIWKLTAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLIDEIILEDEKRDKKLKKIIDDS
HIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVMGTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTEL
AQKLGIKFNNFKSGELKAVPNPTEKLTEAVRIAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKLV
DAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGIKAIF

Specific function: Digestion of cleaved signal peptides. Required for efficient processing of precursors under conditions of hyper-secretion [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=235, Percent_Identity=31.4893617021277, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787527, Length=184, Percent_Identity=27.1739130434783, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 33896; Mature: 33765

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITPDYLIERRQIKSRLLIWKLTAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHH
DEIILEDEKRDKKLKKIIDDSHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVM
HHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCEEEEE
GTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTELAQKLGIKFNNFKSGELKAV
ECEECCCCEEEEECCCEEEECCCEEEECCEEEEEHHHHHHHHHHHCCEECCCCCCCEEEC
PNPTEKLTEAVRIAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCHHHEEEE
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGI
HHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCC
KAIF
EECC
>Mature Secondary Structure 
SITPDYLIERRQIKSRLLIWKLTAIILIAIVFLLVGKDFAPKEVLPINSNEDYIASVLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHH
DEIILEDEKRDKKLKKIIDDSHIKALIVNVNSPGGTVVGAEKIYNILRKISAKKPVVIVM
HHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCEEEEE
GTMAASGGYLISLGGDYIISHNGTITGSIGVILQTAEVTELAQKLGIKFNNFKSGELKAV
ECEECCCCEEEEECCCEEEECCCEEEECCEEEEEHHHHHHHHHHHCCEECCCCCCCEEEC
PNPTEKLTEAVRIAIMDNIEDTYNFFLELVSERRNLPIEEVKKLADGRVYSGRQALKLKL
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCHHHEEEE
VDAIGSEDTALKWLQEVQKINVNLLVKDYQLKPKPKLMDIILEDFDSIAPSFFKNSFNGI
HHHCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCC
KAIF
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 10455123 [H]