Click here to switch to the map view.

The map label for this gene is dacF [H]

Identifier: 229586661

GI number: 229586661

Start: 528510

End: 529430

Strand: Direct

Name: dacF [H]

Synonym: RAF_ORF0498

Alternate gene names: 229586661

Gene position: 528510-529430 (Clockwise)

Preceding gene: 229586660

Following gene: 229586668

Centisome position: 41.34

GC content: 35.07

Gene sequence:

>921_bases
ATGTTTAGGAAATTTTTATTACCTTATATTTTTGTCGTAACATTTTTCTTTGCGCCTATAAGCGAAGCAAAAAAAGCCGT
AAAAAATACCCCTAAACCTCCTGTTCAAACTAGTTTAGTAATTGATGCAAAAAGCGGTAAAGTGTTGCAGGCCCATAATT
CACAAATTCGGATTTACCCTGCTTCTTTAACTAAGCTAATGACTCTATACTTAATGTTTGAAGCAATTGAAGCCGGTAAG
TTACCTTTAGATAAAAAATTACTTGTATCAGCAAAAGCTTCAAAAATGCCACCGTGTAAGCTGGGTTTAAAAGCAGGGGA
AACTATAACCGTTCGAGAGGCAATTAATGGATTAATTATAAAATCTGCTAATGATGCTGCTGTAACGGTTGCTGAGGGCA
TGAAAGGTTCAGAGAAAGCTTTTGCTCATTTAATGAATGTTAAAGCTAAACAGCTCGGTATGAAGAATACCTATTTTAAA
AATGCTTCAGGTTGGCATGACCCATTGCAGCAAACTACCGCAAGAGATATGGCTAAACTTGCTATGGCTTTAAAGAGAGA
TTATCCTAAATTTTATCCGTTATTTGCTAAAACCAGCTTTGTATTTCGTGGAAATGTAGTAAACGGACATAATAGGGTTA
CTAAAAATTATCAAGGTGCAGAAGGGATGAAAACAGGTTTTCATACACCGGCAGGTTATAATTTAGTAACTACTGCTTCT
AGGAACGGTAAATCATTGATTGCTGTTGTTACAGGCGGTAAAAGTGCCGCATCTCGTGATCAAAAGATGATGGGGCTATT
AGATCAGCATTTTAATATTAAGCCTATAGTCCCTAAACCTTCAATCAAGTTTGCCTCTAATAATAAATCTACTACAAAAG
CTAAATCAAAAATAATAAAGAAAAAGAATATACGTAGTTGA

Upstream 100 bases:

>100_bases
GCATTTTTTACTGGATTGCTTCGTCAAAATTTTCAATTTTTCCTCGCAATGACGAAAAATCACACTTGGTTTAATTTGAA
TAATATTTTATTTATTTAAA

Downstream 100 bases:

>100_bases
TATGTATCGTCATTGTGAGACCACAAAGTGGTCGTGGCAATCTCATGAGGTAATACAAAAACTCCTGAGATTGCTTCGTC
GAAACTTAAGTGGCTCCTCG

Product: Penicillin-binding protein dacF precursor

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MFRKFLLPYIFVVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRIYPASLTKLMTLYLMFEAIEAGK
LPLDKKLLVSAKASKMPPCKLGLKAGETITVREAINGLIIKSANDAAVTVAEGMKGSEKAFAHLMNVKAKQLGMKNTYFK
NASGWHDPLQQTTARDMAKLAMALKRDYPKFYPLFAKTSFVFRGNVVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
RNGKSLIAVVTGGKSAASRDQKMMGLLDQHFNIKPIVPKPSIKFASNNKSTTKAKSKIIKKKNIRS

Sequences:

>Translated_306_residues
MFRKFLLPYIFVVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRIYPASLTKLMTLYLMFEAIEAGK
LPLDKKLLVSAKASKMPPCKLGLKAGETITVREAINGLIIKSANDAAVTVAEGMKGSEKAFAHLMNVKAKQLGMKNTYFK
NASGWHDPLQQTTARDMAKLAMALKRDYPKFYPLFAKTSFVFRGNVVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
RNGKSLIAVVTGGKSAASRDQKMMGLLDQHFNIKPIVPKPSIKFASNNKSTTKAKSKIIKKKNIRS
>Mature_306_residues
MFRKFLLPYIFVVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRIYPASLTKLMTLYLMFEAIEAGK
LPLDKKLLVSAKASKMPPCKLGLKAGETITVREAINGLIIKSANDAAVTVAEGMKGSEKAFAHLMNVKAKQLGMKNTYFK
NASGWHDPLQQTTARDMAKLAMALKRDYPKFYPLFAKTSFVFRGNVVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
RNGKSLIAVVTGGKSAASRDQKMMGLLDQHFNIKPIVPKPSIKFASNNKSTTKAKSKIIKKKNIRS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=281, Percent_Identity=35.5871886120996, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1786851, Length=255, Percent_Identity=35.6862745098039, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI87082022, Length=250, Percent_Identity=33.6, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI87082054, Length=270, Percent_Identity=28.5185185185185, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 33612; Mature: 33612

Theoretical pI: Translated: 11.02; Mature: 11.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRKFLLPYIFVVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRIYP
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCEEEEEH
ASLTKLMTLYLMFEAIEAGKLPLDKKLLVSAKASKMPPCKLGLKAGETITVREAINGLII
HHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCHHCCCCCCCEEEHHHHHCEEEE
KSANDAAVTVAEGMKGSEKAFAHLMNVKAKQLGMKNTYFKNASGWHDPLQQTTARDMAKL
ECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AMALKRDYPKFYPLFAKTSFVFRGNVVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
HHHHHHCCCCCEEHHHCEEEEEECCEECCCCHHCCCCCCCCCCCCCCCCCCCCEEEEECC
RNGKSLIAVVTGGKSAASRDQKMMGLLDQHFNIKPIVPKPSIKFASNNKSTTKAKSKIIK
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCCHHHHHHHHHHH
KKNIRS
HHCCCC
>Mature Secondary Structure
MFRKFLLPYIFVVTFFFAPISEAKKAVKNTPKPPVQTSLVIDAKSGKVLQAHNSQIRIYP
CCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEECCCEEEEEH
ASLTKLMTLYLMFEAIEAGKLPLDKKLLVSAKASKMPPCKLGLKAGETITVREAINGLII
HHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCHHCCCCCCCEEEHHHHHCEEEE
KSANDAAVTVAEGMKGSEKAFAHLMNVKAKQLGMKNTYFKNASGWHDPLQQTTARDMAKL
ECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
AMALKRDYPKFYPLFAKTSFVFRGNVVNGHNRVTKNYQGAEGMKTGFHTPAGYNLVTTAS
HHHHHHCCCCCEEHHHCEEEEEECCEECCCCHHCCCCCCCCCCCCCCCCCCCCEEEEECC
RNGKSLIAVVTGGKSAASRDQKMMGLLDQHFNIKPIVPKPSIKFASNNKSTTKAKSKIIK
CCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEECCCCCCEEEECCCCHHHHHHHHHHH
KKNIRS
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]