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The map label for this gene is tme [C]

Identifier: 229586640

GI number: 229586640

Start: 500715

End: 503018

Strand: Direct

Name: tme [C]

Synonym: RAF_ORF0474

Alternate gene names: 229586640

Gene position: 500715-503018 (Clockwise)

Preceding gene: 229586639

Following gene: 229586643

Centisome position: 39.16

GC content: 36.02

Gene sequence:

>2304_bases
ATGGATGAAATGAATAAAATAAATTATGCTGAGGCCCTTGAATATCATGCAAAAGATAAGCCTGGTAAGATCGCTATTTC
TGCAACAAAGTCTTTAGTTACTCAGCAAGATTTATCACTTGCTTATTCCCCAGGGGTTGCGGCTCCTTGCCTTGAAATTG
CTAAAAATTTAGAGGATGTATATAAATATACTGCTCGCGGTAATTTAGTTGCCGTAATCTCTAACGGTACTGCCGTACTT
GGGCTTGGTAATTTAGGAGCTGCCGCTTCAAAACCGGTGATGGAAGGTAAAGCGGTACTATTCAAAAAATTTGCTGATAT
TGATGCAATTGACTTGGAAGTGAATACGGAAGATCCTGAAGAATTTATTAATGCTGTGAAGTATCTCAGTTATAGTTTTG
GGGGTATTAATCTTGAAGATATTAAAGCTCCGGAATGTTTTATTATAGAAGAAAAATTAAAATCTTTAATGGATATACCG
GTCTTTCATGACGATCAGCATGGAACAGCGATTATTACCGCCGCAGGGCTAATAAATGCTGCGTATCTTACTAATCGCAA
GCTTGAAGATTTAAAAATCGTAGTTAACGGAGCAGGTGCAGCAGCTATTGCTTGCATTGATTTATTAATTGCTCTTGGAG
CTGATAAATCAAAGATTATTGTATGTGATACTAAAGGCGTTGTTTACAAAGGACGCCTAGAAGGTATGAATAAATGGAAA
GAGCTATATGCAAGTGATACCAAGTTTAGAACTTTAGCGGAAAGCTTAAATAACGCTGATGTATTTATAGGGTTATCGGT
AAAAGGCGCAGTAACTAAAGATATGGTGAGTAGAATGGCAAGTAATCCGATTATTTTTGCTATGGCTAACCCTGATCCAG
AAATTACTCCGGAAGATATACAACTTGTACGCGATGATGCGATTATAGCAACAGGGCGATCTGATTATAATAATCAGATT
AATAATGTTATGGGATTTCCTTATATTTTTAGAGGAGCATTAGATATAAGAGCTAAGACTATTAATACGGAAATGAAAAT
AGCTGCAGCAAAAGCCATTGCAGATTTAGCCCGTAGACCTGTACCGGAAGAGGTGTATAAAGCTTATTCAGGGCGTAAGA
TGGTTTTTGGTAAGGAATATATCATTCCTGTACCGTTTGATCCTCGTTTAATTACCGTAGTAGCAGCGGCGGTTGCCGTT
GCTGCAATAGAAAGCGGCGTTGCTAGGGTTAAAGATTTTAGTATAGGTAAATATAAGAAAGAATTAGGTAGCAGATTAAA
TCCTACTGCTAACTATATGAATTTCTTAGCTGAAAAAATTCATAATGCACCGCTTAAACGGATTGTTTTTGCTGAAGGTG
AAGAAGAAGAAGTAATTTCTGCCGCTCTTATGATGCGAGACAAGAAATACGGTCATCCGATTATAATTGGTCGTGTTGAA
CGAATTGAGGCTATGTTAAAAAAGATAGGTCAAGATATTAGCTTAGACGGAATTCAAATAATGAATGCGGCCTTAAGCGA
TAGTTTAGAGAAATATACTGATTATTTATATAAAAGACTTCAGAGAAAAGGTTATTTATACCGTGATTGTGCAAAGCTTG
TTAAAACTGATAAGAATATTTTCGCTGCTTGTATGTTAGCATGCGGTGACGGTGATGCTCTTCTAACCGGTGTTACACAA
AGTTATATCGATAGTTTAGAAGATATCATGAAAGTCATTTCACCAAAAACTAATCGTAGCATCCTTGGCTATTCAATTAT
GATTGCTAAAGATAATAATATTATTATTGCCGATAATTGTATTACAGAATATCCAAACAGTTGGGAACTTGCACAAATAG
CAACCCAAACTGCAGAAATTGCTAAAAATATGGGAATCACGCCTCGTGTTGCACTTCTTTCTTTTTCAACATTCGGTAAT
TCTTCTAAGGCAAAAACAGCTCGTATTCGTGAAGCAGTAAATATACTAGATAATTTTAGCAAGGACAAAGAAGAGCTGAG
CGGCATGAAGGTAGATTTTGAATATGATGGAGAGATGTCGGTTAAAGTGGCTTTAGATGGTGATTTACGTAAATTATATA
AATTTTGTAGATTATCAGGATCTGCAAATGTATTAATTATGCCTGGTTTAAATTCAGCTGCTATTTCTACTGAGTTGTTA
CAAGAATTTTCGTCAAACAGCTTTATAGGTCCTATAACAAACGGTTTTGAAAAACCCGTTCAAATACTTCAAGCTGCAGC
AACAGCTAATGAAATACTAAAGATAGCAACTTTTGCTTGCGTTGAAGCTATAAACGAGGTTTAA

Upstream 100 bases:

>100_bases
AATCTCAGGAATGTTGCCTGAGATTGCTTCGTCGATGCCTATTGCGTCTGCTTGCAATGACGAAAAACTAAAACAAACAA
ATAAAACAGGAATTTCTAAA

Downstream 100 bases:

>100_bases
TTACTATATCTTTAAAATCAATTAACTTGTGAGAGGTAAAGTGGCGTCGATTACAGAACTTCATAAAAATATACAAGAAA
TTACAAAAGATTTACGAAAA

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 767; Mature: 767

Protein sequence:

>767_residues
MDEMNKINYAEALEYHAKDKPGKIAISATKSLVTQQDLSLAYSPGVAAPCLEIAKNLEDVYKYTARGNLVAVISNGTAVL
GLGNLGAAASKPVMEGKAVLFKKFADIDAIDLEVNTEDPEEFINAVKYLSYSFGGINLEDIKAPECFIIEEKLKSLMDIP
VFHDDQHGTAIITAAGLINAAYLTNRKLEDLKIVVNGAGAAAIACIDLLIALGADKSKIIVCDTKGVVYKGRLEGMNKWK
ELYASDTKFRTLAESLNNADVFIGLSVKGAVTKDMVSRMASNPIIFAMANPDPEITPEDIQLVRDDAIIATGRSDYNNQI
NNVMGFPYIFRGALDIRAKTINTEMKIAAAKAIADLARRPVPEEVYKAYSGRKMVFGKEYIIPVPFDPRLITVVAAAVAV
AAIESGVARVKDFSIGKYKKELGSRLNPTANYMNFLAEKIHNAPLKRIVFAEGEEEEVISAALMMRDKKYGHPIIIGRVE
RIEAMLKKIGQDISLDGIQIMNAALSDSLEKYTDYLYKRLQRKGYLYRDCAKLVKTDKNIFAACMLACGDGDALLTGVTQ
SYIDSLEDIMKVISPKTNRSILGYSIMIAKDNNIIIADNCITEYPNSWELAQIATQTAEIAKNMGITPRVALLSFSTFGN
SSKAKTARIREAVNILDNFSKDKEELSGMKVDFEYDGEMSVKVALDGDLRKLYKFCRLSGSANVLIMPGLNSAAISTELL
QEFSSNSFIGPITNGFEKPVQILQAAATANEILKIATFACVEAINEV

Sequences:

>Translated_767_residues
MDEMNKINYAEALEYHAKDKPGKIAISATKSLVTQQDLSLAYSPGVAAPCLEIAKNLEDVYKYTARGNLVAVISNGTAVL
GLGNLGAAASKPVMEGKAVLFKKFADIDAIDLEVNTEDPEEFINAVKYLSYSFGGINLEDIKAPECFIIEEKLKSLMDIP
VFHDDQHGTAIITAAGLINAAYLTNRKLEDLKIVVNGAGAAAIACIDLLIALGADKSKIIVCDTKGVVYKGRLEGMNKWK
ELYASDTKFRTLAESLNNADVFIGLSVKGAVTKDMVSRMASNPIIFAMANPDPEITPEDIQLVRDDAIIATGRSDYNNQI
NNVMGFPYIFRGALDIRAKTINTEMKIAAAKAIADLARRPVPEEVYKAYSGRKMVFGKEYIIPVPFDPRLITVVAAAVAV
AAIESGVARVKDFSIGKYKKELGSRLNPTANYMNFLAEKIHNAPLKRIVFAEGEEEEVISAALMMRDKKYGHPIIIGRVE
RIEAMLKKIGQDISLDGIQIMNAALSDSLEKYTDYLYKRLQRKGYLYRDCAKLVKTDKNIFAACMLACGDGDALLTGVTQ
SYIDSLEDIMKVISPKTNRSILGYSIMIAKDNNIIIADNCITEYPNSWELAQIATQTAEIAKNMGITPRVALLSFSTFGN
SSKAKTARIREAVNILDNFSKDKEELSGMKVDFEYDGEMSVKVALDGDLRKLYKFCRLSGSANVLIMPGLNSAAISTELL
QEFSSNSFIGPITNGFEKPVQILQAAATANEILKIATFACVEAINEV
>Mature_767_residues
MDEMNKINYAEALEYHAKDKPGKIAISATKSLVTQQDLSLAYSPGVAAPCLEIAKNLEDVYKYTARGNLVAVISNGTAVL
GLGNLGAAASKPVMEGKAVLFKKFADIDAIDLEVNTEDPEEFINAVKYLSYSFGGINLEDIKAPECFIIEEKLKSLMDIP
VFHDDQHGTAIITAAGLINAAYLTNRKLEDLKIVVNGAGAAAIACIDLLIALGADKSKIIVCDTKGVVYKGRLEGMNKWK
ELYASDTKFRTLAESLNNADVFIGLSVKGAVTKDMVSRMASNPIIFAMANPDPEITPEDIQLVRDDAIIATGRSDYNNQI
NNVMGFPYIFRGALDIRAKTINTEMKIAAAKAIADLARRPVPEEVYKAYSGRKMVFGKEYIIPVPFDPRLITVVAAAVAV
AAIESGVARVKDFSIGKYKKELGSRLNPTANYMNFLAEKIHNAPLKRIVFAEGEEEEVISAALMMRDKKYGHPIIIGRVE
RIEAMLKKIGQDISLDGIQIMNAALSDSLEKYTDYLYKRLQRKGYLYRDCAKLVKTDKNIFAACMLACGDGDALLTGVTQ
SYIDSLEDIMKVISPKTNRSILGYSIMIAKDNNIIIADNCITEYPNSWELAQIATQTAEIAKNMGITPRVALLSFSTFGN
SSKAKTARIREAVNILDNFSKDKEELSGMKVDFEYDGEMSVKVALDGDLRKLYKFCRLSGSANVLIMPGLNSAAISTELL
QEFSSNSFIGPITNGFEKPVQILQAAATANEILKIATFACVEAINEV

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=347, Percent_Identity=29.3948126801153, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI62420880, Length=347, Percent_Identity=29.3948126801153, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI239049447, Length=347, Percent_Identity=29.3948126801153, Blast_Score=80, Evalue=8e-15,
Organism=Homo sapiens, GI4505143, Length=346, Percent_Identity=27.1676300578035, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788806, Length=758, Percent_Identity=45.2506596306069, Blast_Score=623, Evalue=1e-179,
Organism=Escherichia coli, GI1788800, Length=327, Percent_Identity=24.1590214067278, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788635, Length=348, Percent_Identity=24.4252873563218, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI87081919, Length=408, Percent_Identity=23.7745098039216, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=343, Percent_Identity=27.1137026239067, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322823, Length=321, Percent_Identity=29.9065420560748, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24646388, Length=412, Percent_Identity=27.1844660194175, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646386, Length=412, Percent_Identity=27.1844660194175, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356279, Length=362, Percent_Identity=28.4530386740331, Blast_Score=80, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281362672, Length=361, Percent_Identity=28.808864265928, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281362674, Length=364, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 83822; Mature: 83822

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEMNKINYAEALEYHAKDKPGKIAISATKSLVTQQDLSLAYSPGVAAPCLEIAKNLEDV
CCCCHHCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHH
YKYTARGNLVAVISNGTAVLGLGNLGAAASKPVMEGKAVLFKKFADIDAIDLEVNTEDPE
HHHHCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHH
EFINAVKYLSYSFGGINLEDIKAPECFIIEEKLKSLMDIPVFHDDQHGTAIITAAGLINA
HHHHHHHHHHHCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEHHHHHHH
AYLTNRKLEDLKIVVNGAGAAAIACIDLLIALGADKSKIIVCDTKGVVYKGRLEGMNKWK
HHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECHHHHHHHHH
ELYASDTKFRTLAESLNNADVFIGLSVKGAVTKDMVSRMASNPIIFAMANPDPEITPEDI
HHHCCCHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
QLVRDDAIIATGRSDYNNQINNVMGFPYIFRGALDIRAKTINTEMKIAAAKAIADLARRP
HEEECCEEEEECCCCHHHHHHHCCCCCHHHHCHHEEEEEEECCHHHHHHHHHHHHHHHCC
VPEEVYKAYSGRKMVFGKEYIIPVPFDPRLITVVAAAVAVAAIESGVARVKDFSIGKYKK
CCHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ELGSRLNPTANYMNFLAEKIHNAPLKRIVFAEGEEEEVISAALMMRDKKYGHPIIIGRVE
HHCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECHH
RIEAMLKKIGQDISLDGIQIMNAALSDSLEKYTDYLYKRLQRKGYLYRDCAKLVKTDKNI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
FAACMLACGDGDALLTGVTQSYIDSLEDIMKVISPKTNRSILGYSIMIAKDNNIIIADNC
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEEECCC
ITEYPNSWELAQIATQTAEIAKNMGITPRVALLSFSTFGNSSKAKTARIREAVNILDNFS
HHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
KDKEELSGMKVDFEYDGEMSVKVALDGDLRKLYKFCRLSGSANVLIMPGLNSAAISTELL
CCHHHHCCEEEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHH
QEFSSNSFIGPITNGFEKPVQILQAAATANEILKIATFACVEAINEV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDEMNKINYAEALEYHAKDKPGKIAISATKSLVTQQDLSLAYSPGVAAPCLEIAKNLEDV
CCCCHHCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHH
YKYTARGNLVAVISNGTAVLGLGNLGAAASKPVMEGKAVLFKKFADIDAIDLEVNTEDPE
HHHHCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHH
EFINAVKYLSYSFGGINLEDIKAPECFIIEEKLKSLMDIPVFHDDQHGTAIITAAGLINA
HHHHHHHHHHHCCCCCCHHHCCCCCEEEEHHHHHHHHCCCCEECCCCCCEEEEHHHHHHH
AYLTNRKLEDLKIVVNGAGAAAIACIDLLIALGADKSKIIVCDTKGVVYKGRLEGMNKWK
HHHHCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECHHHHHHHHH
ELYASDTKFRTLAESLNNADVFIGLSVKGAVTKDMVSRMASNPIIFAMANPDPEITPEDI
HHHCCCHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCHHHH
QLVRDDAIIATGRSDYNNQINNVMGFPYIFRGALDIRAKTINTEMKIAAAKAIADLARRP
HEEECCEEEEECCCCHHHHHHHCCCCCHHHHCHHEEEEEEECCHHHHHHHHHHHHHHHCC
VPEEVYKAYSGRKMVFGKEYIIPVPFDPRLITVVAAAVAVAAIESGVARVKDFSIGKYKK
CCHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ELGSRLNPTANYMNFLAEKIHNAPLKRIVFAEGEEEEVISAALMMRDKKYGHPIIIGRVE
HHCCCCCCHHHHHHHHHHHHCCCCHHEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECHH
RIEAMLKKIGQDISLDGIQIMNAALSDSLEKYTDYLYKRLQRKGYLYRDCAKLVKTDKNI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHH
FAACMLACGDGDALLTGVTQSYIDSLEDIMKVISPKTNRSILGYSIMIAKDNNIIIADNC
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEEECCC
ITEYPNSWELAQIATQTAEIAKNMGITPRVALLSFSTFGNSSKAKTARIREAVNILDNFS
HHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
KDKEELSGMKVDFEYDGEMSVKVALDGDLRKLYKFCRLSGSANVLIMPGLNSAAISTELL
CCHHHHCCEEEEEEECCCEEEEEEECCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHH
QEFSSNSFIGPITNGFEKPVQILQAAATANEILKIATFACVEAINEV
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]