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The map label for this gene is proP3 [H]

Identifier: 229586643

GI number: 229586643

Start: 504992

End: 506209

Strand: Direct

Name: proP3 [H]

Synonym: RAF_ORF0477

Alternate gene names: 229586643

Gene position: 504992-506209 (Clockwise)

Preceding gene: 229586640

Following gene: 229586645

Centisome position: 39.5

GC content: 31.61

Gene sequence:

>1218_bases
ATGTTTAATTCAGCTTTTTTAGTTGCATTTTTTACAACGATTATCCGTTATTATGATTATGCTCTATTCGGTTTATCGGC
TAGTATTCTTGCACAAAATTTCTTACCTAATATAGAGCAGGATAAACAACTACTTGGTTTTTTTGCTATTCTTAGTGCAG
CTGTTATAGTACGACCTTTAGGGTCCTTAATTTTTGGATTTATCGGAGATAAATACGGGCGTACTAAATCTATCAAGATA
TCAATGTGCATGAGTAGCATTGCTACCGGATTCATTGCTATTACTCCTTCCTTTGAGATAATAGGTATTTTTGCTACGGT
AATACTAACATTATGTCGAATGGTTTTTTTAGCAAGCCTTGCCGGAGAGGTTGACGGAATAAAGGTTTATGTAGCTGAAA
AAACAGGAGATAATCAGAAAAATTTAAATATAGGAATAGTTTCATGCTGTGGCCAAATAGGTGCATTACTTGCGGCTATA
GCTTATTATTACGCTAGTAATTCCGATATCCAATATTTATGGCGTTTTAATTTCTTTATTGGTGCAATATTTGGTTTATT
TGCTATTTTGATGAGAAATTTTTTTAAAGAAAGTAGCGAGTTTTTAAGAACGTCATCAAAAAGTACCTTGCGGCAAATTA
TATGGAATAATAAACTATCCGTTATAATAGCATTACTAATAAACGGTGCTATTGGAGGAGTGTATCACTTTTTAGTAATT
TTCTTAGGAGTTTTTTTAACAAAAATAGCTCTTATAAATCATCCTGAAATAAGATTTATGAATATAGCTCTTACTACTAC
CTATGGAATATCAGCTGTTTTTGCAGGGCTGTTTGCCGATAAAATAAACCCTTTAAGACAGATTACATGGGCTTTATTTG
TCAGCATTATTGTTGCTTTAGGAATGCAAATAATGTTATACAAACAAATATTTAATATTCTCTGTCCCGTTATATTAATA
GGACTTGCAGCATTTTATGCAGTACCGCTACAAATTATTGTTCAATCCTTATTTAAGACTAATGTTAAAATGAGGCTTTA
TAGTCTATCACATTCTTTTGGAGGTATTATTCTATCCTCAACTACTCCTTTCTTTAGTATGTTGTTATGGCAAAATTTTA
AGTCACTATCTCTCACTTTAAGCTTTTTCATATTTTTGCTTATAATATTAATGAGTACAGTGTTGGTTTTACGTAGGATG
TCATCCTCACAACAGTAA

Upstream 100 bases:

>100_bases
ATTTGTAGACGCAAAGAGTCAAAATAGATTAAATTAATTTTCTTTCACACAGCCTAAATAGTTGGTATCATATGCTGTTG
CCACAAAAAGAAAATATTAT

Downstream 100 bases:

>100_bases
AGTCTTGTTGCATGGCTTGAAAAACCTACTCAATGTCATCCCCGCGAAAGCGGGGATCTAGCAAAATAACTTCAATATTG
ATATATTTGCCAAAATAAAC

Product: Proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MFNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPNIEQDKQLLGFFAILSAAVIVRPLGSLIFGFIGDKYGRTKSIKI
SMCMSSIATGFIAITPSFEIIGIFATVILTLCRMVFLASLAGEVDGIKVYVAEKTGDNQKNLNIGIVSCCGQIGALLAAI
AYYYASNSDIQYLWRFNFFIGAIFGLFAILMRNFFKESSEFLRTSSKSTLRQIIWNNKLSVIIALLINGAIGGVYHFLVI
FLGVFLTKIALINHPEIRFMNIALTTTYGISAVFAGLFADKINPLRQITWALFVSIIVALGMQIMLYKQIFNILCPVILI
GLAAFYAVPLQIIVQSLFKTNVKMRLYSLSHSFGGIILSSTTPFFSMLLWQNFKSLSLTLSFFIFLLIILMSTVLVLRRM
SSSQQ

Sequences:

>Translated_405_residues
MFNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPNIEQDKQLLGFFAILSAAVIVRPLGSLIFGFIGDKYGRTKSIKI
SMCMSSIATGFIAITPSFEIIGIFATVILTLCRMVFLASLAGEVDGIKVYVAEKTGDNQKNLNIGIVSCCGQIGALLAAI
AYYYASNSDIQYLWRFNFFIGAIFGLFAILMRNFFKESSEFLRTSSKSTLRQIIWNNKLSVIIALLINGAIGGVYHFLVI
FLGVFLTKIALINHPEIRFMNIALTTTYGISAVFAGLFADKINPLRQITWALFVSIIVALGMQIMLYKQIFNILCPVILI
GLAAFYAVPLQIIVQSLFKTNVKMRLYSLSHSFGGIILSSTTPFFSMLLWQNFKSLSLTLSFFIFLLIILMSTVLVLRRM
SSSQQ
>Mature_405_residues
MFNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPNIEQDKQLLGFFAILSAAVIVRPLGSLIFGFIGDKYGRTKSIKI
SMCMSSIATGFIAITPSFEIIGIFATVILTLCRMVFLASLAGEVDGIKVYVAEKTGDNQKNLNIGIVSCCGQIGALLAAI
AYYYASNSDIQYLWRFNFFIGAIFGLFAILMRNFFKESSEFLRTSSKSTLRQIIWNNKLSVIIALLINGAIGGVYHFLVI
FLGVFLTKIALINHPEIRFMNIALTTTYGISAVFAGLFADKINPLRQITWALFVSIIVALGMQIMLYKQIFNILCPVILI
GLAAFYAVPLQIIVQSLFKTNVKMRLYSLSHSFGGIILSSTTPFFSMLLWQNFKSLSLTLSFFIFLLIILMSTVLVLRRM
SSSQQ

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=211, Percent_Identity=28.436018957346, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788292, Length=230, Percent_Identity=29.5652173913043, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788942, Length=396, Percent_Identity=22.4747474747475, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789941, Length=400, Percent_Identity=21.25, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45108; Mature: 45108

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNSAFLVAFFTTIIRYYDYALFGLSASILAQNFLPNIEQDKQLLGFFAILSAAVIVRPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
GSLIFGFIGDKYGRTKSIKISMCMSSIATGFIAITPSFEIIGIFATVILTLCRMVFLASL
HHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AGEVDGIKVYVAEKTGDNQKNLNIGIVSCCGQIGALLAAIAYYYASNSDIQYLWRFNFFI
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
GAIFGLFAILMRNFFKESSEFLRTSSKSTLRQIIWNNKLSVIIALLINGAIGGVYHFLVI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FLGVFLTKIALINHPEIRFMNIALTTTYGISAVFAGLFADKINPLRQITWALFVSIIVAL
HHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GMQIMLYKQIFNILCPVILIGLAAFYAVPLQIIVQSLFKTNVKMRLYSLSHSFGGIILSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEHHCCCCCEEEEC
TTPFFSMLLWQNFKSLSLTLSFFIFLLIILMSTVLVLRRMSSSQQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]