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The map label for this gene is tolB

Identifier: 229586559

GI number: 229586559

Start: 406867

End: 408201

Strand: Direct

Name: tolB

Synonym: RAF_ORF0380

Alternate gene names: 229586559

Gene position: 406867-408201 (Clockwise)

Preceding gene: 229586558

Following gene: 229586561

Centisome position: 31.82

GC content: 37.15

Gene sequence:

>1335_bases
GTGAGAAATATTATATATTTTATATTATCACTATTATTTAGCGTTACCAGCTATGCACTTGAAACAATAAACATTGAGCA
TGGGCGGGCTGACCCAACACCTATAGCGGTCAATAAATTTGATGCCGATAATTCTGCCGCTGATGTATTAGGTCACGATA
TGGTTAAAGTGATCTCTAATGATTTAAAGCTTTCGGGGTTATTCCGTCCTATTTCTGCTGCCTCTTTTATAGAAGAGAAG
ACAGGAATAGAATATAAACCGCTTTTTGCTGCATGGCGTCAAATTAATGCTAGTCTTTTAGTAAACGGTGAAGTTAAAAA
ACTAGAAAGCGGTAAATTTAAAGTTAGCTTTATATTATGGGATACGTTGCTTGAAAAACAGCTCGCCGGTGAAATGCTTG
AAGTACCGAAAAATCTATGGAGAAGAGCAGCACATAAAATTGCCGATAAAATTTATGAAAAAATTACCGGTGATGCCGGT
TATTTTGACACTAAAATAGTGTATGTATCAGAAAGCAGCTCTTTACCGAAAATAAAAAGAATTGCTTTGATGGATTATGA
CGGGGCTAATAATAAATACCTTACCAATGGAAAGTCACTAGTCTTAACCCCAAGATTTGCTCGTTCGGCGGATAAGATTT
TTTATGTTTCATATGCTACTAAAAGAAGAGTACTTGTTTATGAAAAAGATTTAAAAACAGGCAAAGAAAGTGTAGTAGGT
GATTTTCCCGGTATATCTTTTGCTCCTAGATTCTCACCAGACGGTAGAAAAGCTGTAATGTCGATAGCTAAAAACGGCTC
GACGCATATTTATGAAATCGACCTTGCTACTAAACGGCTTCATAAATTAACTGACGGATTCGGTATTAATACCTCTCCTA
GCTATTCACCGGACGGTAAAAAAATTGTATATAATTCCGATAGAAACGGTGTGCCTCAATTATATATCATGAATTCAGAC
GGAAGTGACGTTCAGCGTATTAGTTTTGGCGGTGGTTCTTACGCAGCTCCTAGCTGGTCGCCTAGAGGAGACTATATAGC
ATTTACTAAGATTACTAAAGGAGACGGAGGAAAAACTTTTAATATCGGAATTATGAAAGCATGTCCTCAAGATGATGAGA
ATAGTGAAAAGATAATAACAAGCGGATATTTAGTTGAAAGTCCTTGTTGGTCGCCTAACGGACGAGTTATTATGTTTGCT
AAAGGTTGGCCGTCGAGTGCTAAAGCTCCGGGAAAAAACAAAATTTTTGCAATTGATCTAACAGGTCATAATGAAAGAGA
AATTATGACACCGGCAGATGCTTCTGATCCAGAATGGTCAGGTGTACTGAACTAA

Upstream 100 bases:

>100_bases
AAGAACTGATGAAGATTTTTGGGTAATATATGTCATTCCTGTGAAAGCAGGAATGACATACAACAACCATAACGGAATTT
GATAAAAAGGAGTATTTTAA

Downstream 100 bases:

>100_bases
TGTCATTCCTACGAAAGCATTGTTGGGTGGATCGTTTTTCCGTTGTCATACCGTGACTTAATCGCGGTATCCAGTAAAAA
TACTATTAGTTGTTTTTGGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRNIIYFILSLLFSVTSYALETINIEHGRADPTPIAVNKFDADNSAADVLGHDMVKVISNDLKLSGLFRPISAASFIEEK
TGIEYKPLFAAWRQINASLLVNGEVKKLESGKFKVSFILWDTLLEKQLAGEMLEVPKNLWRRAAHKIADKIYEKITGDAG
YFDTKIVYVSESSSLPKIKRIALMDYDGANNKYLTNGKSLVLTPRFARSADKIFYVSYATKRRVLVYEKDLKTGKESVVG
DFPGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGKKIVYNSDRNGVPQLYIMNSD
GSDVQRISFGGGSYAAPSWSPRGDYIAFTKITKGDGGKTFNIGIMKACPQDDENSEKIITSGYLVESPCWSPNGRVIMFA
KGWPSSAKAPGKNKIFAIDLTGHNEREIMTPADASDPEWSGVLN

Sequences:

>Translated_444_residues
MRNIIYFILSLLFSVTSYALETINIEHGRADPTPIAVNKFDADNSAADVLGHDMVKVISNDLKLSGLFRPISAASFIEEK
TGIEYKPLFAAWRQINASLLVNGEVKKLESGKFKVSFILWDTLLEKQLAGEMLEVPKNLWRRAAHKIADKIYEKITGDAG
YFDTKIVYVSESSSLPKIKRIALMDYDGANNKYLTNGKSLVLTPRFARSADKIFYVSYATKRRVLVYEKDLKTGKESVVG
DFPGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGKKIVYNSDRNGVPQLYIMNSD
GSDVQRISFGGGSYAAPSWSPRGDYIAFTKITKGDGGKTFNIGIMKACPQDDENSEKIITSGYLVESPCWSPNGRVIMFA
KGWPSSAKAPGKNKIFAIDLTGHNEREIMTPADASDPEWSGVLN
>Mature_444_residues
MRNIIYFILSLLFSVTSYALETINIEHGRADPTPIAVNKFDADNSAADVLGHDMVKVISNDLKLSGLFRPISAASFIEEK
TGIEYKPLFAAWRQINASLLVNGEVKKLESGKFKVSFILWDTLLEKQLAGEMLEVPKNLWRRAAHKIADKIYEKITGDAG
YFDTKIVYVSESSSLPKIKRIALMDYDGANNKYLTNGKSLVLTPRFARSADKIFYVSYATKRRVLVYEKDLKTGKESVVG
DFPGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGKKIVYNSDRNGVPQLYIMNSD
GSDVQRISFGGGSYAAPSWSPRGDYIAFTKITKGDGGKTFNIGIMKACPQDDENSEKIITSGYLVESPCWSPNGRVIMFA
KGWPSSAKAPGKNKIFAIDLTGHNEREIMTPADASDPEWSGVLN

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=400, Percent_Identity=28.75, Blast_Score=168, Evalue=8e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_RICAE (C3PN07)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845060.1
- ProteinModelPortal:   C3PN07
- GeneID:   7815055
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0380
- OMA:   RVADYDG
- ProtClustDB:   PRK05137
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 49080; Mature: 49080

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNIIYFILSLLFSVTSYALETINIEHGRADPTPIAVNKFDADNSAADVLGHDMVKVISN
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
DLKLSGLFRPISAASFIEEKTGIEYKPLFAAWRQINASLLVNGEVKKLESGKFKVSFILW
CCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHEECCCCEEEEEEEH
DTLLEKQLAGEMLEVPKNLWRRAAHKIADKIYEKITGDAGYFDTKIVYVSESSSLPKIKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEE
IALMDYDGANNKYLTNGKSLVLTPRFARSADKIFYVSYATKRRVLVYEKDLKTGKESVVG
EEEEECCCCCCCEEECCCEEEEECHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHCC
DFPGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGK
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCC
KIVYNSDRNGVPQLYIMNSDGSDVQRISFGGGSYAAPSWSPRGDYIAFTKITKGDGGKTF
EEEECCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCEE
NIGIMKACPQDDENSEKIITSGYLVESPCWSPNGRVIMFAKGWPSSAKAPGKNKIFAIDL
EEEEEEECCCCCCCCCEEEEECEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEE
TGHNEREIMTPADASDPEWSGVLN
CCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRNIIYFILSLLFSVTSYALETINIEHGRADPTPIAVNKFDADNSAADVLGHDMVKVISN
CCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHC
DLKLSGLFRPISAASFIEEKTGIEYKPLFAAWRQINASLLVNGEVKKLESGKFKVSFILW
CCEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCHHEECCCCEEEEEEEH
DTLLEKQLAGEMLEVPKNLWRRAAHKIADKIYEKITGDAGYFDTKIVYVSESSSLPKIKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEE
IALMDYDGANNKYLTNGKSLVLTPRFARSADKIFYVSYATKRRVLVYEKDLKTGKESVVG
EEEEECCCCCCCEEECCCEEEEECHHHCCCCCEEEEEECCCCEEEEEECCCCCCCHHHCC
DFPGISFAPRFSPDGRKAVMSIAKNGSTHIYEIDLATKRLHKLTDGFGINTSPSYSPDGK
CCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCC
KIVYNSDRNGVPQLYIMNSDGSDVQRISFGGGSYAAPSWSPRGDYIAFTKITKGDGGKTF
EEEECCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCEE
NIGIMKACPQDDENSEKIITSGYLVESPCWSPNGRVIMFAKGWPSSAKAPGKNKIFAIDL
EEEEEEECCCCCCCCCEEEEECEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEE
TGHNEREIMTPADASDPEWSGVLN
CCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA