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The map label for this gene is petB [H]

Identifier: 229586521

GI number: 229586521

Start: 362391

End: 363587

Strand: Direct

Name: petB [H]

Synonym: RAF_ORF0333

Alternate gene names: 229586521

Gene position: 362391-363587 (Clockwise)

Preceding gene: 229586520

Following gene: 229586522

Centisome position: 28.34

GC content: 39.01

Gene sequence:

>1197_bases
ATGAATGAAGATATTACCCCTAAAAAGCCTAATGCTATTATAGAATGGATAGATTATCGTCTTCCTATTTTTGCATTCCT
AAAGCATTTTAGTCATTATCAAACTCCGAAAAATCTTAGCTATTTGTGGAATTTAGGCTCTATTGCCGGTATTGCGTTAG
TGATTCAGATAATCACCGGCGTCATACTTGCTATGCACTATACTCCGCATGTTGATCATGCTTTCGATAGTGTTGAGCGA
ATCATGCGTAACGTGAATTACGGTTGGTTACTGCGTTATACTCATGCGGTAGGTGCATCGATGTTTTTTGCCGCCGTATA
CTTACACATAGCAAGAGGTTTGTATTACGGCTCTTATAAAGCACCTAGAGAACTGCTTTGGCATATCGGTATCATCATTT
TTCTAACCATGATGGCTACTGCTTTCATGGGTTACGTGCTTCCTTGGGGGCAGATGAGCTACTGGGGAGCAACGGTTATT
ACTAATTTATTTTCCGCTATTCCGTTAATAGGGGAATTCATAGTTACGTGGTTATGGGGTGGTTTTTCTGTTGATAACCC
GACATTAAATAGGTTCTTCTCACTGCATTATTTATTGCCGTTTATTATTGTTGCTTTAGTGATGCTGCATTTAGTGGCCT
TGCATCAGCATGGTTCAAATAACCCAAAAGGTATAGATGTTAAAAGCCCTAAAGATACTATTCCGTTCCACCCTTATTAT
ACCGTAAAGGATTTTGTCGGTTTCGGGGTTTATTTCATAATATTTGCCTATTTTATTTTTTACGAACCTAATTATTTAGG
ACATCCTGATAATTACATTCCAGCAAATCCGTTAGTTACGCCTGCCCACATTGTCCCTGAATGGTATTTTCTGCCGTTTT
ATGCAATACTTAGAGCCATACCGTCTAAGCTCGGTGGGGTGCTATTAATGTTCGGAAGTATATTTGTGTTATTTTTACTA
CCTTGGCTTGATACTTCAAAAGTTAGAAGTAGCAATTATAGACCGATATACCGAATGGCTTTTTGGATATTTATGGCAGA
TTGTTTATTGCTTGGTTATTTAGGTGGGCAGCCGGCAGAAGAGCCGTATATTACCATTAGCCGTTTTGCTGCTTGTTATT
ACTTTTTTCATGTCTTAGTAGCTCTGCCGCTGATAGGTAAATATGAAAAACCGCTGCCCTTGCCGGAGGAGTTGTAA

Upstream 100 bases:

>100_bases
TTCTTCAGGTAGAGTTAGAAGAGGTCCTGCTCCTTTAAATTTAGCTGTGCCGCCTTACACTTTTATTAGCGACAAAAAAA
TTAGAATAGGGTAATTATTT

Downstream 100 bases:

>100_bases
GAGTTAAGTCATTGCGAAGAGATGTCATTGCGAGGAAATTGCATAGCAATTGACGAAGCAATCTCAGGAGTTTGTTATTA
TTTCATGAGATTGCCGTGCA

Product: Cytochrome b

Products: Q; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MNEDITPKKPNAIIEWIDYRLPIFAFLKHFSHYQTPKNLSYLWNLGSIAGIALVIQIITGVILAMHYTPHVDHAFDSVER
IMRNVNYGWLLRYTHAVGASMFFAAVYLHIARGLYYGSYKAPRELLWHIGIIIFLTMMATAFMGYVLPWGQMSYWGATVI
TNLFSAIPLIGEFIVTWLWGGFSVDNPTLNRFFSLHYLLPFIIVALVMLHLVALHQHGSNNPKGIDVKSPKDTIPFHPYY
TVKDFVGFGVYFIIFAYFIFYEPNYLGHPDNYIPANPLVTPAHIVPEWYFLPFYAILRAIPSKLGGVLLMFGSIFVLFLL
PWLDTSKVRSSNYRPIYRMAFWIFMADCLLLGYLGGQPAEEPYITISRFAACYYFFHVLVALPLIGKYEKPLPLPEEL

Sequences:

>Translated_398_residues
MNEDITPKKPNAIIEWIDYRLPIFAFLKHFSHYQTPKNLSYLWNLGSIAGIALVIQIITGVILAMHYTPHVDHAFDSVER
IMRNVNYGWLLRYTHAVGASMFFAAVYLHIARGLYYGSYKAPRELLWHIGIIIFLTMMATAFMGYVLPWGQMSYWGATVI
TNLFSAIPLIGEFIVTWLWGGFSVDNPTLNRFFSLHYLLPFIIVALVMLHLVALHQHGSNNPKGIDVKSPKDTIPFHPYY
TVKDFVGFGVYFIIFAYFIFYEPNYLGHPDNYIPANPLVTPAHIVPEWYFLPFYAILRAIPSKLGGVLLMFGSIFVLFLL
PWLDTSKVRSSNYRPIYRMAFWIFMADCLLLGYLGGQPAEEPYITISRFAACYYFFHVLVALPLIGKYEKPLPLPEEL
>Mature_398_residues
MNEDITPKKPNAIIEWIDYRLPIFAFLKHFSHYQTPKNLSYLWNLGSIAGIALVIQIITGVILAMHYTPHVDHAFDSVER
IMRNVNYGWLLRYTHAVGASMFFAAVYLHIARGLYYGSYKAPRELLWHIGIIIFLTMMATAFMGYVLPWGQMSYWGATVI
TNLFSAIPLIGEFIVTWLWGGFSVDNPTLNRFFSLHYLLPFIIVALVMLHLVALHQHGSNNPKGIDVKSPKDTIPFHPYY
TVKDFVGFGVYFIIFAYFIFYEPNYLGHPDNYIPANPLVTPAHIVPEWYFLPFYAILRAIPSKLGGVLLMFGSIFVLFLL
PWLDTSKVRSSNYRPIYRMAFWIFMADCLLLGYLGGQPAEEPYITISRFAACYYFFHVLVALPLIGKYEKPLPLPEEL

Specific function: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis [H]

COG id: COG1290

COG function: function code C; Cytochrome b subunit of the bc complex

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b family [H]

Homologues:

Organism=Homo sapiens, GI251831119, Length=372, Percent_Identity=52.6881720430108, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6226529, Length=357, Percent_Identity=55.4621848739496, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6226532, Length=233, Percent_Identity=57.9399141630901, Blast_Score=282, Evalue=8e-77,
Organism=Saccharomyces cerevisiae, GI6226531, Length=152, Percent_Identity=58.5526315789474, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6226530, Length=136, Percent_Identity=51.4705882352941, Blast_Score=149, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016175
- InterPro:   IPR005798
- InterPro:   IPR005797
- InterPro:   IPR016174 [H]

Pfam domain/function: PF00032 Cytochrom_B_C; PF00033 Cytochrom_B_N [H]

EC number: 1.10.2.2

Molecular weight: Translated: 45784; Mature: 45784

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS51002 CYTB_NTER ; PS51003 CYTB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: QH2; ferricytochrome c

Specific reaction: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA