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The map label for this gene is rne [H]

Identifier: 229586506

GI number: 229586506

Start: 345136

End: 347208

Strand: Direct

Name: rne [H]

Synonym: RAF_ORF0318

Alternate gene names: 229586506

Gene position: 345136-347208 (Clockwise)

Preceding gene: 229586503

Following gene: 229586507

Centisome position: 26.99

GC content: 32.75

Gene sequence:

>2073_bases
ATGAATAAAAAGATTATAATTGATGCTAATTTTCCAAGCGAAACAAGAGTAGTTTTATTAGGACAAAGCAACAATATAGA
AGATATTGAATTTCAAACAACCGTAAGACAACAAAATAAAGGTAACATTTATTTAGCAAAAGTTACAAGAATAGAACCGT
CGCTACAAGCTGTTTTTATAGAGTACGGAATGGATAAAAGCGGTTTTTTACCGTTTAGTGAAATCCATCCTAACTATTAC
AATTTGCCTACCTCGGAGAGAAATTTTCCGGTTAATGCTTTTCCTGAAATAGCTCTTTCAAATATTACGGTTGAGGATGA
TAAAGAGAAACCGGCTGTTATATATGATTCTTTGGTTGATAGTGAAGAAATTGACCTAAAAACCATAGAAGATTTAGTAG
AGAGTACGTTCCAGTCAGAACTTAATTTAGAAGCGGCTGATGATATTGAAATGATTCAAAGCGGTACCCCTGAGTCAAAT
ATTCCGCAATATAAACAATATAAAGTTCAAGAAGTAATAAGAAAAAACCAAATATTATTAGTGCAGGTTACTAAGGAAGA
ACGAGGAAATAAGTGCGCCGCTTTTACTACTTATATATCCTTGGCTGGTAAATATTGTGTTTTAATGCCTAATAAAGGTT
CGCAAAACGGTATATCGCGTAAAATATCAAACGGGGAAGAAAGAAAAAGACTGAAAGATATTTTAAATAAAATAGTAAGC
GGTGACAAAAATGCTTATAGCGTCATTGTTAGAACCGCAGGTAGAGGAAGTAGCACCTTAGATTTAAAGAAAGACTATAA
TTATTTAGCAAGGTTATGGAATAAAATTCGTAAAAGTACTATTAAATTTCCGGCTCCGTGTTTTATTCATGAGGAAGATA
GTATAATACGTAAAACCATACGTGATATGTGCGATCACAATGTTAAAGAGGTGGTAATTCAAGGACAAGAGGGTTATGCA
GATGCTTCAAAATTTATGCAGGATTTATTACCTTCAGAGCTTGTAAAGCTGAAAGAGCATAAAAGTAAAACTCCTATATT
TACTAAGTTTCAAGTAGAAGAGCAATTAGTTAAATTATATCAGCCTGTAGTGACGTTACCTTCAGGAGGATATATCGTTA
TTAATCCCACGGAAGCTCTTATTGCAATTGATGTAAATTCCGGTAAATCAACTTCTGAACAAAATATTGAAGAAACGGCA
CTAAAAACTAATTTAGAAGCAGCCAAAGAATTAGCAAAGCAAGTAAAGCTTAGAGATCTATCAGGTTTGATAGTTGTTGA
TTTTATAGACATGAGCGAAGCTAAGAATCGTAAAATTATTGAACGATCCTTTAAAGAGTTTTTAAGTCGTGATCGTGCTC
GTATACAAACCGGTAATATTAGTCAGTTTGGACTACTTGAATTCTCAAGGCAGAGATTACGTTCTTCTTTCTTAGAAACT
AATGCGGCAATTTGTTCTCATTGTAACGGTAAAGGAGTCATTAGAGCAAGTGACGCTAATGCTATGTTAATTTTACGTAC
TATAGAGAATGAAATTTTTGAAGAAAGAATTGATGTAATAAATGTTTTTACCAATATCGTTTCAGTTCTTTATCTACTTA
ATAATAAACGTGCTGAGATAAAATTTATTGAAGAAAAATATAATATAAAGCTTAATTTTTATTCCGACCCTAATGCTACA
TCAGATAGTTATTCGATTGAAAAAGTGAAGCTGTTTAAGAAAAATAACAATAATTTTAATTCTGTTAAGCCGGTAATTCA
GAATCATAATGCCGATTATACTGCAGAAGATCTGAAAAAAGAACAGTTACGCAAAAATAAACATAAATGGAAAACAGCTA
ATAATAGTAATAATGTTGCTAATGAAGTAAAAGATAAGAGTTTAGAGGCAGAAGAATCGGTGGAGCAAACCGTAAGCGTT
GTGGAAGAGGTGCAAGTTGTAGAAAGTAATGCCGGCTTAGAAGAAGCACAAGCTGCTCCAAAAAAGACAAAACGTAGATA
TCGTAATAAAACATCAAATAAGAAATGTCCTTCTAATACCGTCGAAGATACGGAAACGAATGCAGAGTCTTGA

Upstream 100 bases:

>100_bases
ATTATCTATTTAAATAAATTAATACAAGATAAACAATGATTTTTCTTAAGATACAAGCATTATATTAATTATTTGTTCTT
CAAGTTAGTGAGCAAGATAA

Downstream 100 bases:

>100_bases
TTTGAGGGCTAATATGTCATTCCTGCGAAAGCAGGAATCCAGTAATCCTTAATGTCACCCCGTGGTCAAGCCCACGGGGT
CCATAAAAATAATAAAAAAT

Product: Ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 690; Mature: 690

Protein sequence:

>690_residues
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFIEYGMDKSGFLPFSEIHPNYY
NLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSLVDSEEIDLKTIEDLVESTFQSELNLEAADDIEMIQSGTPESN
IPQYKQYKVQEVIRKNQILLVQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGSQNGISRKISNGEERKRLKDILNKIVS
GDKNAYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTIRDMCDHNVKEVVIQGQEGYA
DASKFMQDLLPSELVKLKEHKSKTPIFTKFQVEEQLVKLYQPVVTLPSGGYIVINPTEALIAIDVNSGKSTSEQNIEETA
LKTNLEAAKELAKQVKLRDLSGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
NAAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEIKFIEEKYNIKLNFYSDPNAT
SDSYSIEKVKLFKKNNNNFNSVKPVIQNHNADYTAEDLKKEQLRKNKHKWKTANNSNNVANEVKDKSLEAEESVEQTVSV
VEEVQVVESNAGLEEAQAAPKKTKRRYRNKTSNKKCPSNTVEDTETNAES

Sequences:

>Translated_690_residues
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFIEYGMDKSGFLPFSEIHPNYY
NLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSLVDSEEIDLKTIEDLVESTFQSELNLEAADDIEMIQSGTPESN
IPQYKQYKVQEVIRKNQILLVQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGSQNGISRKISNGEERKRLKDILNKIVS
GDKNAYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTIRDMCDHNVKEVVIQGQEGYA
DASKFMQDLLPSELVKLKEHKSKTPIFTKFQVEEQLVKLYQPVVTLPSGGYIVINPTEALIAIDVNSGKSTSEQNIEETA
LKTNLEAAKELAKQVKLRDLSGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
NAAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEIKFIEEKYNIKLNFYSDPNAT
SDSYSIEKVKLFKKNNNNFNSVKPVIQNHNADYTAEDLKKEQLRKNKHKWKTANNSNNVANEVKDKSLEAEESVEQTVSV
VEEVQVVESNAGLEEAQAAPKKTKRRYRNKTSNKKCPSNTVEDTETNAES
>Mature_690_residues
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFIEYGMDKSGFLPFSEIHPNYY
NLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSLVDSEEIDLKTIEDLVESTFQSELNLEAADDIEMIQSGTPESN
IPQYKQYKVQEVIRKNQILLVQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGSQNGISRKISNGEERKRLKDILNKIVS
GDKNAYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTIRDMCDHNVKEVVIQGQEGYA
DASKFMQDLLPSELVKLKEHKSKTPIFTKFQVEEQLVKLYQPVVTLPSGGYIVINPTEALIAIDVNSGKSTSEQNIEETA
LKTNLEAAKELAKQVKLRDLSGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
NAAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEIKFIEEKYNIKLNFYSDPNAT
SDSYSIEKVKLFKKNNNNFNSVKPVIQNHNADYTAEDLKKEQLRKNKHKWKTANNSNNVANEVKDKSLEAEESVEQTVSV
VEEVQVVESNAGLEEAQAAPKKTKRRYRNKTSNKKCPSNTVEDTETNAES

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=391, Percent_Identity=41.1764705882353, Blast_Score=312, Evalue=5e-86,
Organism=Escherichia coli, GI87082235, Length=346, Percent_Identity=31.5028901734104, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 78383; Mature: 78383

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFI
CCCEEEEECCCCCCCEEEEEECCCCCCCEEEHHHHHHCCCCCEEEEEEEECCCCHHEEEE
EYGMDKSGFLPFSEIHPNYYNLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSLVD
ECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHEECEEEECCCCCCCEEEHHHCC
SEEIDLKTIEDLVESTFQSELNLEAADDIEMIQSGTPESNIPQYKQYKVQEVIRKNQILL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEE
VQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGSQNGISRKISNGEERKRLKDILNKIVS
EEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHC
GDKNAYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTI
CCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
RDMCDHNVKEVVIQGQEGYADASKFMQDLLPSELVKLKEHKSKTPIFTKFQVEEQLVKLY
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
QPVVTLPSGGYIVINPTEALIAIDVNSGKSTSEQNIEETALKTNLEAAKELAKQVKLRDL
CCCEECCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHHHHHC
NAAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEI
CHHHHHCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KFIEEKYNIKLNFYSDPNATSDSYSIEKVKLFKKNNNNFNSVKPVIQNHNADYTAEDLKK
EEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHH
EQLRKNKHKWKTANNSNNVANEVKDKSLEAEESVEQTVSVVEEVQVVESNAGLEEAQAAP
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCH
KKTKRRYRNKTSNKKCPSNTVEDTETNAES
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKKIIIDANFPSETRVVLLGQSNNIEDIEFQTTVRQQNKGNIYLAKVTRIEPSLQAVFI
CCCEEEEECCCCCCCEEEEEECCCCCCCEEEHHHHHHCCCCCEEEEEEEECCCCHHEEEE
EYGMDKSGFLPFSEIHPNYYNLPTSERNFPVNAFPEIALSNITVEDDKEKPAVIYDSLVD
ECCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCHHEECEEEECCCCCCCEEEHHHCC
SEEIDLKTIEDLVESTFQSELNLEAADDIEMIQSGTPESNIPQYKQYKVQEVIRKNQILL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEE
VQVTKEERGNKCAAFTTYISLAGKYCVLMPNKGSQNGISRKISNGEERKRLKDILNKIVS
EEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHC
GDKNAYSVIVRTAGRGSSTLDLKKDYNYLARLWNKIRKSTIKFPAPCFIHEEDSIIRKTI
CCCCEEEEEEEECCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHH
RDMCDHNVKEVVIQGQEGYADASKFMQDLLPSELVKLKEHKSKTPIFTKFQVEEQLVKLY
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHH
QPVVTLPSGGYIVINPTEALIAIDVNSGKSTSEQNIEETALKTNLEAAKELAKQVKLRDL
CCCEECCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGLIVVDFIDMSEAKNRKIIERSFKEFLSRDRARIQTGNISQFGLLEFSRQRLRSSFLET
CCEEEEEEECCCCCCCHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHHHHHHHHHHHHC
NAAICSHCNGKGVIRASDANAMLILRTIENEIFEERIDVINVFTNIVSVLYLLNNKRAEI
CHHHHHCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
KFIEEKYNIKLNFYSDPNATSDSYSIEKVKLFKKNNNNFNSVKPVIQNHNADYTAEDLKK
EEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHH
EQLRKNKHKWKTANNSNNVANEVKDKSLEAEESVEQTVSVVEEVQVVESNAGLEEAQAAP
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCH
KKTKRRYRNKTSNKKCPSNTVEDTETNAES
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]