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The map label for this gene is ddl [H]

Identifier: 229586498

GI number: 229586498

Start: 336879

End: 337844

Strand: Direct

Name: ddl [H]

Synonym: RAF_ORF0310

Alternate gene names: 229586498

Gene position: 336879-337844 (Clockwise)

Preceding gene: 229586497

Following gene: 229586499

Centisome position: 26.35

GC content: 34.06

Gene sequence:

>966_bases
GTGCATAAATATCAAACACATTGGGTAGAACACTCTATAGTCAAAATATTAAGTAGTACCGGTAAAAAGCATATAGCATT
AATGGTAGGCGGTATGTCTGCTGAGCGTGAAGTATCGTTAGTGTCAAGTGAAGGAGTTAGTAAAGCTTTAATTGAATTAG
GATATAGAGTTACCTTTATTGATATGGGAGCGGATATTGCAGTTAGATTACAAGAAATAAAACCTGATATCGTTTTTAAT
TGCCTGCACGGTACATATGGTGAAGACGGTTGTTTACCAGGATTACTTAATATAATGCGGATTCCTTATACTCATAGCGG
CATGTTATCTTCAGCTTTAGCATTCGATAAAATACATTCTAGGATCTGGTTTTTAACGAATAATATTAATATGGCAGAAA
GCATTGTTGTAAATAAAAGCGATAATATAAAAAATGATCCTATGAAACGACCGTACGTTATTAAGCCTCTTACGCAAGGT
TCAAGTATTGGTGTTGAGGTGATATTTGCAGAAGACGATTTTAACTTTGCCGATTATGATTTTCCTTACGGTGATCAGGT
AATAATAGAACAATATATAAAAGGGCGAGAATTGCAAGTAGCGGTATTAAACGGCAAAGCTCTTGGAGCTTTAGAGATTA
AATTACTAAAGAATCGTTTCTATGATTATGAAACTAAATATACCGAAGGATTTGCTGATCATCTTTGTCCTGCTCCGCTA
CCTGCTAATTTATATGAGAAATTACTTATAGAGTCAGAAAAAATTTATAAAACAATGAATTGTAAAGGTCCTGCTAGAGC
AGAGTTTATTTTAGAAGAGCAGACAAACAAGTTATATGCTTTGGAGATAAATACTCACCCAGGTATGACGCCTTTATCAA
TAGTGCCGGAAATTGCAGCTTATGCAGGGATAAATTTTACTAACTTAATTGAAGAAATTATAAAGATGGCAAGCTTTGAA
TCATGA

Upstream 100 bases:

>100_bases
CCCGCCTACGCGGGGATGACATCGAGTAGGTTTTTCGAGCTATGCAACAAGGTCGCTTTTAGTTAGGAATTACAATGGAT
TTAATAAGAAAGGAAAAAAC

Downstream 100 bases:

>100_bases
GAAAAAAAACAAGCTCCAATAAAAAAAAACAAACAAAAAAAACTAATAATATCTCATTACGCCGAAAATTAGGACTAATT
TACAAAAAAGCAATATTAGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MHKYQTHWVEHSIVKILSSTGKKHIALMVGGMSAEREVSLVSSEGVSKALIELGYRVTFIDMGADIAVRLQEIKPDIVFN
CLHGTYGEDGCLPGLLNIMRIPYTHSGMLSSALAFDKIHSRIWFLTNNINMAESIVVNKSDNIKNDPMKRPYVIKPLTQG
SSIGVEVIFAEDDFNFADYDFPYGDQVIIEQYIKGRELQVAVLNGKALGALEIKLLKNRFYDYETKYTEGFADHLCPAPL
PANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAYAGINFTNLIEEIIKMASFE
S

Sequences:

>Translated_321_residues
MHKYQTHWVEHSIVKILSSTGKKHIALMVGGMSAEREVSLVSSEGVSKALIELGYRVTFIDMGADIAVRLQEIKPDIVFN
CLHGTYGEDGCLPGLLNIMRIPYTHSGMLSSALAFDKIHSRIWFLTNNINMAESIVVNKSDNIKNDPMKRPYVIKPLTQG
SSIGVEVIFAEDDFNFADYDFPYGDQVIIEQYIKGRELQVAVLNGKALGALEIKLLKNRFYDYETKYTEGFADHLCPAPL
PANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAYAGINFTNLIEEIIKMASFE
S
>Mature_321_residues
MHKYQTHWVEHSIVKILSSTGKKHIALMVGGMSAEREVSLVSSEGVSKALIELGYRVTFIDMGADIAVRLQEIKPDIVFN
CLHGTYGEDGCLPGLLNIMRIPYTHSGMLSSALAFDKIHSRIWFLTNNINMAESIVVNKSDNIKNDPMKRPYVIKPLTQG
SSIGVEVIFAEDDFNFADYDFPYGDQVIIEQYIKGRELQVAVLNGKALGALEIKLLKNRFYDYETKYTEGFADHLCPAPL
PANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAAYAGINFTNLIEEIIKMASFE
S

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=34.983498349835, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=30.8571428571429, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 36022; Mature: 36022

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKYQTHWVEHSIVKILSSTGKKHIALMVGGMSAEREVSLVSSEGVSKALIELGYRVTFI
CCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEHHCCCHHHHHHHCCCEEEEE
DMGADIAVRLQEIKPDIVFNCLHGTYGEDGCLPGLLNIMRIPYTHSGMLSSALAFDKIHS
ECCCCEEEHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RIWFLTNNINMAESIVVNKSDNIKNDPMKRPYVIKPLTQGSSIGVEVIFAEDDFNFADYD
EEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCC
FPYGDQVIIEQYIKGRELQVAVLNGKALGALEIKLLKNRFYDYETKYTEGFADHLCPAPL
CCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHEEHHHHCCCCCCHHCCCHHHHCCCCCC
PANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAA
CHHHHHHHHHCHHHHHHHCCCCCCCHHEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHH
YAGINFTNLIEEIIKMASFES
HCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHKYQTHWVEHSIVKILSSTGKKHIALMVGGMSAEREVSLVSSEGVSKALIELGYRVTFI
CCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEEEHHCCCHHHHHHHCCCEEEEE
DMGADIAVRLQEIKPDIVFNCLHGTYGEDGCLPGLLNIMRIPYTHSGMLSSALAFDKIHS
ECCCCEEEHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RIWFLTNNINMAESIVVNKSDNIKNDPMKRPYVIKPLTQGSSIGVEVIFAEDDFNFADYD
EEEEEECCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCC
FPYGDQVIIEQYIKGRELQVAVLNGKALGALEIKLLKNRFYDYETKYTEGFADHLCPAPL
CCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHEEHHHHCCCCCCHHCCCHHHHCCCCCC
PANLYEKLLIESEKIYKTMNCKGPARAEFILEEQTNKLYALEINTHPGMTPLSIVPEIAA
CHHHHHHHHHCHHHHHHHCCCCCCCHHEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHH
YAGINFTNLIEEIIKMASFES
HCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA