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The map label for this gene is parE [H]

Identifier: 229586475

GI number: 229586475

Start: 313506

End: 315494

Strand: Direct

Name: parE [H]

Synonym: RAF_ORF0287

Alternate gene names: 229586475

Gene position: 313506-315494 (Clockwise)

Preceding gene: 229586474

Following gene: 229586476

Centisome position: 24.52

GC content: 33.94

Gene sequence:

>1989_bases
ATGAGCGATTTATTCAGCTTTAACAAAGAAAAGAAAAATAAGCTAGTCGATAATAACTATAGTGCAAAAGATATTGAAGT
ATTAGAGGGGCTTGAGCCTGTTCGTAAAAGACCGGGGATGTATATCGGGGGTACTGATTTAAATGCTATGCATCATTTAG
TATCTGAGGTGCTGGATAATGCTATGGATGAGGCAGTCGCCGGCTTTGCAAGTATCATCACGATAACAATGCATCATGAT
CATAGTATTACTATATTTGATAACGGTCGCGGTATCCCTATTGATAATCATCCTAAATTTCCTGACAAATCAGCTTTAGA
AGTAATTTTAACTACTCTTCATTCAGGAGGTAAATTCTCAAATAATGTTTATCATACTGCAGGCGGATTGCATGGTGTTG
GAATATCGGTAGTAAATGCTTTATCTAAACATTTAGAAATAAAGGTTTATAAACAAGGTAAATTATATAGCCAAAGTTAT
TCTAAAGGAGAAAAATTAACTGATTTAATATGTGAAGAAGCATCTAAAAGGCTAAGAGGTACATCAATAAATTTCACTCC
TGATCCGGAAATTTTTAGTGAAAAATTACATTTTAATCCTAAAAAGATTTATGAGCTTGCAAGGTCAAAAGCTTATTTAT
ATCGCGGTGTTACTATAGAATGGGCATGCGAAGTAGAAGTACAGTCAGATATACCTAAAAAGGCATTAATAAGTTTCCCA
AATGGTTTAAAAGATTATTTAAGTTCAAAAATAACTTTAGATAATTTAGTTATTCCGGGAATTTTTGCAGGTAATATAGA
GTCTACGTCGGACAAGATAAAACTTGAATGGGCGATTTGTTGGCAAAATAACGATAGCTCGGCATTTGTTCAATCATATT
GTAATACTGTTCCGACTCCTCAAGGAGGAACACATGAGCAAGGACTTAAGTCGGCTATTTTACGTGGGCTTAAAGCATAT
GGTGAAATGATAGGGAATAAAAAAGCTGCTAATCTAACTATTGAGGATATTTTAGAAACCGCAAGTGTCGTACTTTCTAT
TTTTATAACTGAGCCGTCTTTTCAAGGACAAACTAAGGAAAAATTAGTATCAAACGGTGTAAGTAAACCGGTCGAGAATA
TAATAAAAGATCATTTTGACCATTTTCTTAGTAGCGATAAAGCTTTAGCTACTAATTTACTTGAGCATGTAATTAGCATT
GCTGAGTTTAGAATCAGTAAGAAAAACGAAAAAAATATTTCCCGTAAAAATGCCACTCAAAAATTACGTTTACCCGGTAA
ACTTGCCGATTGCACACGCACTTCACCTGAAGGGACGGAATTATTTATTGTAGAAGGTGATTCGGCAGGAGGCTCTGCAA
AACAAGCACGTAATAGAGAAACGCAAGCAGTATTACCGCTATGGGGTAAAGTTCTAAATGTTGCGAGTTCTACGCTTGAG
AAAATCGTTAATAATCAAGCAATACAAGATTTAGAAATAGCTCTTGCCTGCGGTAGTTTAAAGAATTATAAAAAAGAAAA
TCTGCGTTACGAAAAAATAATTATTATGACTGATGCAGATGTTGACGGTGCTCATATAGCTTCGTTATTAATGACTTTCT
TTTTTTTACGAATGCCGAAATTGGTAGAAGAGGGGCATTTATATCTAGCTAAGCCTCCGCTTTATCGTTTAACGCAGTCT
AATAAAACTTATTATGCAGGCGATGAAGAGGAAAAAGCTAAGTTAACGGATAAATTATTAAAAGCTAGTAAAGCTAAAAT
TGAAGTAGGTAGGTTTAAAGGTCTCGGTGAAATGATGCCTGCACAATTAAAGGAAACTACTATGCATCCGGAAAAAAGAT
CGCTTTTAAAGGTAACTTTAGCAGATTTTCAAAATGTTGATAAAATAGTAGATGATTTAATGGGTAAAAAGCCGGAAAAA
AGATTTCAATTTATTTATGAACAGGCTTTAGTTAAAATGGATAAAATTATTAGTGAGCTGGATATTTAA

Upstream 100 bases:

>100_bases
ATTACGTAGAATTTTCAAACAATTTCTTTAAAGCTTAATTGCTTTTCTATGTTAAATAGTTTAATTTAGGAGAGAAAATA
ATTAATTAAATCTCTAATAT

Downstream 100 bases:

>100_bases
TCGTTATTGCGAGGAGCCGTAGGTGACGCGGCAATCTCGTCAAATATCCTAAGATTACTTCGTAATTTTCCTCGCAATGA
CGCTTTGCTTAAAAAACACA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSDLFSFNKEKKNKLVDNNYSAKDIEVLEGLEPVRKRPGMYIGGTDLNAMHHLVSEVLDNAMDEAVAGFASIITITMHHD
HSITIFDNGRGIPIDNHPKFPDKSALEVILTTLHSGGKFSNNVYHTAGGLHGVGISVVNALSKHLEIKVYKQGKLYSQSY
SKGEKLTDLICEEASKRLRGTSINFTPDPEIFSEKLHFNPKKIYELARSKAYLYRGVTIEWACEVEVQSDIPKKALISFP
NGLKDYLSSKITLDNLVIPGIFAGNIESTSDKIKLEWAICWQNNDSSAFVQSYCNTVPTPQGGTHEQGLKSAILRGLKAY
GEMIGNKKAANLTIEDILETASVVLSIFITEPSFQGQTKEKLVSNGVSKPVENIIKDHFDHFLSSDKALATNLLEHVISI
AEFRISKKNEKNISRKNATQKLRLPGKLADCTRTSPEGTELFIVEGDSAGGSAKQARNRETQAVLPLWGKVLNVASSTLE
KIVNNQAIQDLEIALACGSLKNYKKENLRYEKIIIMTDADVDGAHIASLLMTFFFLRMPKLVEEGHLYLAKPPLYRLTQS
NKTYYAGDEEEKAKLTDKLLKASKAKIEVGRFKGLGEMMPAQLKETTMHPEKRSLLKVTLADFQNVDKIVDDLMGKKPEK
RFQFIYEQALVKMDKIISELDI

Sequences:

>Translated_662_residues
MSDLFSFNKEKKNKLVDNNYSAKDIEVLEGLEPVRKRPGMYIGGTDLNAMHHLVSEVLDNAMDEAVAGFASIITITMHHD
HSITIFDNGRGIPIDNHPKFPDKSALEVILTTLHSGGKFSNNVYHTAGGLHGVGISVVNALSKHLEIKVYKQGKLYSQSY
SKGEKLTDLICEEASKRLRGTSINFTPDPEIFSEKLHFNPKKIYELARSKAYLYRGVTIEWACEVEVQSDIPKKALISFP
NGLKDYLSSKITLDNLVIPGIFAGNIESTSDKIKLEWAICWQNNDSSAFVQSYCNTVPTPQGGTHEQGLKSAILRGLKAY
GEMIGNKKAANLTIEDILETASVVLSIFITEPSFQGQTKEKLVSNGVSKPVENIIKDHFDHFLSSDKALATNLLEHVISI
AEFRISKKNEKNISRKNATQKLRLPGKLADCTRTSPEGTELFIVEGDSAGGSAKQARNRETQAVLPLWGKVLNVASSTLE
KIVNNQAIQDLEIALACGSLKNYKKENLRYEKIIIMTDADVDGAHIASLLMTFFFLRMPKLVEEGHLYLAKPPLYRLTQS
NKTYYAGDEEEKAKLTDKLLKASKAKIEVGRFKGLGEMMPAQLKETTMHPEKRSLLKVTLADFQNVDKIVDDLMGKKPEK
RFQFIYEQALVKMDKIISELDI
>Mature_661_residues
SDLFSFNKEKKNKLVDNNYSAKDIEVLEGLEPVRKRPGMYIGGTDLNAMHHLVSEVLDNAMDEAVAGFASIITITMHHDH
SITIFDNGRGIPIDNHPKFPDKSALEVILTTLHSGGKFSNNVYHTAGGLHGVGISVVNALSKHLEIKVYKQGKLYSQSYS
KGEKLTDLICEEASKRLRGTSINFTPDPEIFSEKLHFNPKKIYELARSKAYLYRGVTIEWACEVEVQSDIPKKALISFPN
GLKDYLSSKITLDNLVIPGIFAGNIESTSDKIKLEWAICWQNNDSSAFVQSYCNTVPTPQGGTHEQGLKSAILRGLKAYG
EMIGNKKAANLTIEDILETASVVLSIFITEPSFQGQTKEKLVSNGVSKPVENIIKDHFDHFLSSDKALATNLLEHVISIA
EFRISKKNEKNISRKNATQKLRLPGKLADCTRTSPEGTELFIVEGDSAGGSAKQARNRETQAVLPLWGKVLNVASSTLEK
IVNNQAIQDLEIALACGSLKNYKKENLRYEKIIIMTDADVDGAHIASLLMTFFFLRMPKLVEEGHLYLAKPPLYRLTQSN
KTYYAGDEEEKAKLTDKLLKASKAKIEVGRFKGLGEMMPAQLKETTMHPEKRSLLKVTLADFQNVDKIVDDLMGKKPEKR
FQFIYEQALVKMDKIISELDI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=643, Percent_Identity=24.8833592534992, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI19913408, Length=631, Percent_Identity=24.5641838351823, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1789408, Length=639, Percent_Identity=41.7840375586854, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=40.1785714285714, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17535065, Length=585, Percent_Identity=23.9316239316239, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI212645845, Length=564, Percent_Identity=24.468085106383, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324241, Length=706, Percent_Identity=25.070821529745, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17136538, Length=599, Percent_Identity=24.0400667779633, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: 5.99.1.3

Molecular weight: Translated: 73799; Mature: 73667

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLFSFNKEKKNKLVDNNYSAKDIEVLEGLEPVRKRPGMYIGGTDLNAMHHLVSEVLDN
CCCCHHCCHHHHHHCCCCCCCCHHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHH
AMDEAVAGFASIITITMHHDHSITIFDNGRGIPIDNHPKFPDKSALEVILTTLHSGGKFS
HHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
NNVYHTAGGLHGVGISVVNALSKHLEIKVYKQGKLYSQSYSKGEKLTDLICEEASKRLRG
CCEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHCC
TSINFTPDPEIFSEKLHFNPKKIYELARSKAYLYRGVTIEWACEVEVQSDIPKKALISFP
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHEEECCEEEEEEEEECCCCCCHHHHHCCC
NGLKDYLSSKITLDNLVIPGIFAGNIESTSDKIKLEWAICWQNNDSSAFVQSYCNTVPTP
CHHHHHHHCCCCCCCEEECCEEECCCCCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCC
QGGTHEQGLKSAILRGLKAYGEMIGNKKAANLTIEDILETASVVLSIFITEPSFQGQTKE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHH
KLVSNGVSKPVENIIKDHFDHFLSSDKALATNLLEHVISIAEFRISKKNEKNISRKNATQ
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHCCCH
KLRLPGKLADCTRTSPEGTELFIVEGDSAGGSAKQARNRETQAVLPLWGKVLNVASSTLE
HCCCCCCHHHCCCCCCCCCEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KIVNNQAIQDLEIALACGSLKNYKKENLRYEKIIIMTDADVDGAHIASLLMTFFFLRMPK
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LVEEGHLYLAKPPLYRLTQSNKTYYAGDEEEKAKLTDKLLKASKAKIEVGRFKGLGEMMP
HHHCCCEEEECCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHEEHHHHCCCHHHHH
AQLKETTMHPEKRSLLKVTLADFQNVDKIVDDLMGKKPEKRFQFIYEQALVKMDKIISEL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DI
CC
>Mature Secondary Structure 
SDLFSFNKEKKNKLVDNNYSAKDIEVLEGLEPVRKRPGMYIGGTDLNAMHHLVSEVLDN
CCCHHCCHHHHHHCCCCCCCCHHHHHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHHH
AMDEAVAGFASIITITMHHDHSITIFDNGRGIPIDNHPKFPDKSALEVILTTLHSGGKFS
HHHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
NNVYHTAGGLHGVGISVVNALSKHLEIKVYKQGKLYSQSYSKGEKLTDLICEEASKRLRG
CCEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHCC
TSINFTPDPEIFSEKLHFNPKKIYELARSKAYLYRGVTIEWACEVEVQSDIPKKALISFP
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHEEECCEEEEEEEEECCCCCCHHHHHCCC
NGLKDYLSSKITLDNLVIPGIFAGNIESTSDKIKLEWAICWQNNDSSAFVQSYCNTVPTP
CHHHHHHHCCCCCCCEEECCEEECCCCCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCC
QGGTHEQGLKSAILRGLKAYGEMIGNKKAANLTIEDILETASVVLSIFITEPSFQGQTKE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCHHH
KLVSNGVSKPVENIIKDHFDHFLSSDKALATNLLEHVISIAEFRISKKNEKNISRKNATQ
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHCCCH
KLRLPGKLADCTRTSPEGTELFIVEGDSAGGSAKQARNRETQAVLPLWGKVLNVASSTLE
HCCCCCCHHHCCCCCCCCCEEEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KIVNNQAIQDLEIALACGSLKNYKKENLRYEKIIIMTDADVDGAHIASLLMTFFFLRMPK
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LVEEGHLYLAKPPLYRLTQSNKTYYAGDEEEKAKLTDKLLKASKAKIEVGRFKGLGEMMP
HHHCCCEEEECCCHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHEEHHHHCCCHHHHH
AQLKETTMHPEKRSLLKVTLADFQNVDKIVDDLMGKKPEKRFQFIYEQALVKMDKIISEL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
DI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA