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The map label for this gene is def [H]

Identifier: 229586451

GI number: 229586451

Start: 281890

End: 282417

Strand: Direct

Name: def [H]

Synonym: RAF_ORF0257

Alternate gene names: 229586451

Gene position: 281890-282417 (Clockwise)

Preceding gene: 229586450

Following gene: 229586452

Centisome position: 22.05

GC content: 35.8

Gene sequence:

>528_bases
ATGTCAATATTACCGATAGTAACGGCACCTGATGAAAGATTAAAGCAGAAATCTCAGCCGGTTTTAGAGTTTACCGATCA
AACACGAAAATTTATGGATGATATGCTTAAAACTATGTACCATGAAGACGGTGCAGGGCTTGCAGCAGTACAAGTAGGGG
TATTGAAACGTATTTTAGTAATTGATATACAAGATCATGATTCGGTAGCAAGACCAAAAGATTTTTATCCGCTTTTTATA
GTGAATCCTGAAATGATAGAACAAGCAGAAGAGCTAGTTACGGCTAACGAAGGTTGTATCTCATTACCGGAGCAACGTAT
TGAGGTGGCAAGACCGGAATCTATAAAGATTAGGTATTTAGACTATCATGGTAAATCGCAAGAGCTTAAAGCAAATGATT
GGCTTGCAAGAGTTATCCAGCATGAGTATGATCATTTAGAAGGTAAGCTTATGATTGATTATTTAAGTAATTTAAAACGA
GATGTAGTACTTCGTAAGCTTAAGAAACTCAAAAATAATATAGTGTGA

Upstream 100 bases:

>100_bases
GTGAGTTTATTATGCAACTATTGCCGGAATTAGGCAGGTTTGCTTTCTTTACATTTTGGTGATTTTATAGTATATTAAAA
TTAAATATTAAGATTTAATT

Downstream 100 bases:

>100_bases
AAGTAATCTTTATGGGAACGCCTGAATTCGCTGTTCCCGCTCTTAAAAAACTTATAACTCATCACGAAGTTAAAGCTGTT
TTTACACAGCAACCTAAAGC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MSILPIVTAPDERLKQKSQPVLEFTDQTRKFMDDMLKTMYHEDGAGLAAVQVGVLKRILVIDIQDHDSVARPKDFYPLFI
VNPEMIEQAEELVTANEGCISLPEQRIEVARPESIKIRYLDYHGKSQELKANDWLARVIQHEYDHLEGKLMIDYLSNLKR
DVVLRKLKKLKNNIV

Sequences:

>Translated_175_residues
MSILPIVTAPDERLKQKSQPVLEFTDQTRKFMDDMLKTMYHEDGAGLAAVQVGVLKRILVIDIQDHDSVARPKDFYPLFI
VNPEMIEQAEELVTANEGCISLPEQRIEVARPESIKIRYLDYHGKSQELKANDWLARVIQHEYDHLEGKLMIDYLSNLKR
DVVLRKLKKLKNNIV
>Mature_174_residues
SILPIVTAPDERLKQKSQPVLEFTDQTRKFMDDMLKTMYHEDGAGLAAVQVGVLKRILVIDIQDHDSVARPKDFYPLFIV
NPEMIEQAEELVTANEGCISLPEQRIEVARPESIKIRYLDYHGKSQELKANDWLARVIQHEYDHLEGKLMIDYLSNLKRD
VVLRKLKKLKNNIV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=31.25, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=45.8823529411765, Blast_Score=145, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=32.8571428571429, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24645728, Length=140, Percent_Identity=30, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20270; Mature: 20139

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILPIVTAPDERLKQKSQPVLEFTDQTRKFMDDMLKTMYHEDGAGLAAVQVGVLKRILV
CCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHE
IDIQDHDSVARPKDFYPLFIVNPEMIEQAEELVTANEGCISLPEQRIEVARPESIKIRYL
EEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEE
DYHGKSQELKANDWLARVIQHEYDHLEGKLMIDYLSNLKRDVVLRKLKKLKNNIV
ECCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SILPIVTAPDERLKQKSQPVLEFTDQTRKFMDDMLKTMYHEDGAGLAAVQVGVLKRILV
CCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHE
IDIQDHDSVARPKDFYPLFIVNPEMIEQAEELVTANEGCISLPEQRIEVARPESIKIRYL
EEECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEE
DYHGKSQELKANDWLARVIQHEYDHLEGKLMIDYLSNLKRDVVLRKLKKLKNNIV
ECCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]