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The map label for this gene is fmt

Identifier: 229586452

GI number: 229586452

Start: 282414

End: 283325

Strand: Direct

Name: fmt

Synonym: RAF_ORF0258

Alternate gene names: 229586452

Gene position: 282414-283325 (Clockwise)

Preceding gene: 229586451

Following gene: 229586453

Centisome position: 22.09

GC content: 34.21

Gene sequence:

>912_bases
GTGAAAGTAATCTTTATGGGAACGCCTGAATTCGCTGTTCCCGCTCTTAAAAAACTTATAACTCATCACGAAGTTAAAGC
TGTTTTTACACAGCAACCTAAAGCTAAGGGCAGAGGACTTAACTTAGCTAAATCTCCAATACATCAATTAGCATTTGAGC
ATCAAATTCCTGTTTATACTCCTTCTACTCTTCGTAATGATGAAATAATAAATCTAATTAATAAAGTTAATGCTGATATT
ATAGTTGTTATTGCCTATGGTTTTATTGTGCCGAAAGCTATATTAGAAGCTAAGAAATACGGCTGTCTTAATATCCATCC
ATCCGATTTGCCTCGTCACAGAGGAGCGGCTCCTCTGCAGCGTACTATTATAGAGGGTGACCGGAAAAGTAGTGTATGTA
TTATGCGTATGGATACAGGGCTTGATACAGGTGATATATTGATGAAAGAAGATTTTGATTTAGAAGAAAGAATAACATTG
GAAGAACTGCATAACAAATGTGCAAATCTCGGAGCGGAGTTATTAATTAAAACACTTGCAAATATCGATAATATAGTACC
AATAACACAGCCAAGTGACGGCGTTACGTATGCTCATAAATTAACTAAAGAAGAAGGAAAAATTAATTGGCATGAATCCG
CGTATAAAATAGATTGTAAAATAAGGGGCATGAATCCTTGGCCTGGAGCATATTTTAGTTATAATGATAAAATAATTAAA
ATCCTTGAAGCAGAATATTTAAATGCGGATCATCACTTTACTTCCGGTACCGTTATTAGCGATAAGCTGGAAATAGCTTG
TGGAAGCGGTATATTACGAGTGAAAAAACTTCAGCAAGAGAGTAAAAAAGCTTTGAATATAGAAGAATTTTTACGTGGTA
CAAATATTTTAAAAGATACAGTATTAAAATAA

Upstream 100 bases:

>100_bases
AGTATGATCATTTAGAAGGTAAGCTTATGATTGATTATTTAAGTAATTTAAAACGAGATGTAGTACTTCGTAAGCTTAAG
AAACTCAAAAATAATATAGT

Downstream 100 bases:

>100_bases
GAGTATTATTATGGGTAAAGATAAAAAAGAAGTTAGGTTTAATGATAATTGAGACGTTAAGGAGTTTACCTTAAAGTTTG
AAAAGAATAGTGATATTTAT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKVIFMGTPEFAVPALKKLITHHEVKAVFTQQPKAKGRGLNLAKSPIHQLAFEHQIPVYTPSTLRNDEIINLINKVNADI
IVVIAYGFIVPKAILEAKKYGCLNIHPSDLPRHRGAAPLQRTIIEGDRKSSVCIMRMDTGLDTGDILMKEDFDLEERITL
EELHNKCANLGAELLIKTLANIDNIVPITQPSDGVTYAHKLTKEEGKINWHESAYKIDCKIRGMNPWPGAYFSYNDKIIK
ILEAEYLNADHHFTSGTVISDKLEIACGSGILRVKKLQQESKKALNIEEFLRGTNILKDTVLK

Sequences:

>Translated_303_residues
MKVIFMGTPEFAVPALKKLITHHEVKAVFTQQPKAKGRGLNLAKSPIHQLAFEHQIPVYTPSTLRNDEIINLINKVNADI
IVVIAYGFIVPKAILEAKKYGCLNIHPSDLPRHRGAAPLQRTIIEGDRKSSVCIMRMDTGLDTGDILMKEDFDLEERITL
EELHNKCANLGAELLIKTLANIDNIVPITQPSDGVTYAHKLTKEEGKINWHESAYKIDCKIRGMNPWPGAYFSYNDKIIK
ILEAEYLNADHHFTSGTVISDKLEIACGSGILRVKKLQQESKKALNIEEFLRGTNILKDTVLK
>Mature_303_residues
MKVIFMGTPEFAVPALKKLITHHEVKAVFTQQPKAKGRGLNLAKSPIHQLAFEHQIPVYTPSTLRNDEIINLINKVNADI
IVVIAYGFIVPKAILEAKKYGCLNIHPSDLPRHRGAAPLQRTIIEGDRKSSVCIMRMDTGLDTGDILMKEDFDLEERITL
EELHNKCANLGAELLIKTLANIDNIVPITQPSDGVTYAHKLTKEEGKINWHESAYKIDCKIRGMNPWPGAYFSYNDKIIK
ILEAEYLNADHHFTSGTVISDKLEIACGSGILRVKKLQQESKKALNIEEFLRGTNILKDTVLK

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=319, Percent_Identity=28.8401253918495, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI238814322, Length=230, Percent_Identity=30.8695652173913, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI21614513, Length=217, Percent_Identity=31.7972350230415, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1789683, Length=297, Percent_Identity=40.4040404040404, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1788589, Length=279, Percent_Identity=26.1648745519713, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI133930964, Length=259, Percent_Identity=30.1158301158301, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319458, Length=282, Percent_Identity=28.3687943262411, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550868, Length=314, Percent_Identity=28.6624203821656, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=30.6666666666667, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585660, Length=243, Percent_Identity=28.3950617283951, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_RICAE (C3PMQ0)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844953.1
- ProteinModelPortal:   C3PMQ0
- SMR:   C3PMQ0
- GeneID:   7815258
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0258
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34001; Mature: 34001

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIFMGTPEFAVPALKKLITHHEVKAVFTQQPKAKGRGLNLAKSPIHQLAFEHQIPVYT
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PSTLRNDEIINLINKVNADIIVVIAYGFIVPKAILEAKKYGCLNIHPSDLPRHRGAAPLQ
CCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHH
RTIIEGDRKSSVCIMRMDTGLDTGDILMKEDFDLEERITLEELHNKCANLGAELLIKTLA
HHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIDNIVPITQPSDGVTYAHKLTKEEGKINWHESAYKIDCKIRGMNPWPGAYFSYNDKIIK
CCCCEEECCCCCCCCHHHHHHHHCCCCEEECCCEEEEEEEEECCCCCCCCCEECCCCEEE
ILEAEYLNADHHFTSGTVISDKLEIACGSGILRVKKLQQESKKALNIEEFLRGTNILKDT
EHHHHHCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCHHHHHH
VLK
HCC
>Mature Secondary Structure
MKVIFMGTPEFAVPALKKLITHHEVKAVFTQQPKAKGRGLNLAKSPIHQLAFEHQIPVYT
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
PSTLRNDEIINLINKVNADIIVVIAYGFIVPKAILEAKKYGCLNIHPSDLPRHRGAAPLQ
CCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHH
RTIIEGDRKSSVCIMRMDTGLDTGDILMKEDFDLEERITLEELHNKCANLGAELLIKTLA
HHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIDNIVPITQPSDGVTYAHKLTKEEGKINWHESAYKIDCKIRGMNPWPGAYFSYNDKIIK
CCCCEEECCCCCCCCHHHHHHHHCCCCEEECCCEEEEEEEEECCCCCCCCCEECCCCEEE
ILEAEYLNADHHFTSGTVISDKLEIACGSGILRVKKLQQESKKALNIEEFLRGTNILKDT
EHHHHHCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHCHHHHHH
VLK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA