Click here to switch to the map view.

The map label for this gene is hemN [C]

Identifier: 229586416

GI number: 229586416

Start: 229311

End: 230513

Strand: Direct

Name: hemN [C]

Synonym: RAF_ORF0212

Alternate gene names: 229586416

Gene position: 229311-230513 (Clockwise)

Preceding gene: 229586415

Following gene: 229586417

Centisome position: 17.94

GC content: 31.09

Gene sequence:

>1203_bases
ATGTCCACAAAACCTAAGTTAGCACTAGCAAAAATACAAAATATGAGAGTTATGGCTAATGATTTAAGTATTTATATTCA
TTGGCCTTTTTGTTTGTCCAAATGTCCGTATTGTGATTTTAATTCTCATGTAGCAAGTACTATAGATCATAATCAGTGGC
TTAAATCTTATGAAACTGAAATTGAATATTTTAAGGATATTATTCAAAATAAATATATAAAATCTATTTTTTTTGGGGGC
GGTACTCCCTCTTTAATGAATCCTGTAATAGTTGAAGGAATAATAAATAAAATCAGTAATCTAGCAATTATTGATAATCA
AACCGAGATAACTCTAGAAACTAATCCCACATCATTTGAAACTGAAAAATTTAAAGCATTCAAAGCCGCCGGAATTAATC
GTATTTCAATAGGAGTGCAATCCTTAAAAGAGGACGATTTAAAAAAATTAGGTAGAACTCATGATTGTATGCAGGCCATT
AAAACAATTGAGGCGGCAAATACGATTTTTCTACGAGTGTCATTTGATTTAATATATGCACGTAGCGGTCAGACATTAAA
AGACTGGCAAGAAGAATTAAAACAAGCTATGCAGCTCGCTACCTCACATATTTCTCTTTATCAATTGACTATCGAAAAAG
GTACGCCGTGTTATAAATTATTTAAGGAGGGTAATCTGATTTTGCCGCATTCAGATGCGGCAGCTGAAATGTATGAATGG
ACTAATCATTATCTAGAATCTAAAAAGTATTTTAGATATGAAATATCCAATTATGCTATGATAGGGTATGAATGTTTGCA
TAATTTAACTTATTGGAATTATAATAGCTATTTAGGTATAGGCCCCGGAGCTCATAGTAGAATCATTGAATCCTCAAGTT
CGGTATCGGCAATTATGATGTGGCATAAACCTGAAAAATGGTTAGATGCGGTTAAAACAAAAAATGTTGGTATTCAAACT
AATACTAATTTAACACATCAAGAGATTATTGAAGAAATGCTAATGATGGGTTTACGCCTTAGCAAAGGTATAAATATTTC
TACATTAGAGCAGAAATTAAACACGAAATTAGAGAATATTTTAGATATGAATAATCTTAAGCATTATCAAGCGTTAGACT
TAATTAGACTAGATGAAAATATCTATCTTACCGACAAAGGTTTAATGCTGTATAGCTACATAGTACCTAGATTGATTATA
TGA

Upstream 100 bases:

>100_bases
TAGCGAAAATTAATAAGATTTTTGTAGAAATAGTTATTCATATTTCAAAAAAATCTTATAATTTGTAGCAAAAAATAACC
AAAATTAAATCTTTTTAGCA

Downstream 100 bases:

>100_bases
AAATTATTCAAGTAATTTTAAAGTGTATAATTATTTTTATTATTGCTTCTATTCATGTTATGGAATGTAAACTTGAGTTT
AAAAAAGATATATGTGAATG

Product: coproporphyrinogen III oxidase

Products: Protoporphyrinogen IX; CO2; H2O

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSTKPKLALAKIQNMRVMANDLSIYIHWPFCLSKCPYCDFNSHVASTIDHNQWLKSYETEIEYFKDIIQNKYIKSIFFGG
GTPSLMNPVIVEGIINKISNLAIIDNQTEITLETNPTSFETEKFKAFKAAGINRISIGVQSLKEDDLKKLGRTHDCMQAI
KTIEAANTIFLRVSFDLIYARSGQTLKDWQEELKQAMQLATSHISLYQLTIEKGTPCYKLFKEGNLILPHSDAAAEMYEW
TNHYLESKKYFRYEISNYAMIGYECLHNLTYWNYNSYLGIGPGAHSRIIESSSSVSAIMMWHKPEKWLDAVKTKNVGIQT
NTNLTHQEIIEEMLMMGLRLSKGINISTLEQKLNTKLENILDMNNLKHYQALDLIRLDENIYLTDKGLMLYSYIVPRLII

Sequences:

>Translated_400_residues
MSTKPKLALAKIQNMRVMANDLSIYIHWPFCLSKCPYCDFNSHVASTIDHNQWLKSYETEIEYFKDIIQNKYIKSIFFGG
GTPSLMNPVIVEGIINKISNLAIIDNQTEITLETNPTSFETEKFKAFKAAGINRISIGVQSLKEDDLKKLGRTHDCMQAI
KTIEAANTIFLRVSFDLIYARSGQTLKDWQEELKQAMQLATSHISLYQLTIEKGTPCYKLFKEGNLILPHSDAAAEMYEW
TNHYLESKKYFRYEISNYAMIGYECLHNLTYWNYNSYLGIGPGAHSRIIESSSSVSAIMMWHKPEKWLDAVKTKNVGIQT
NTNLTHQEIIEEMLMMGLRLSKGINISTLEQKLNTKLENILDMNNLKHYQALDLIRLDENIYLTDKGLMLYSYIVPRLII
>Mature_399_residues
STKPKLALAKIQNMRVMANDLSIYIHWPFCLSKCPYCDFNSHVASTIDHNQWLKSYETEIEYFKDIIQNKYIKSIFFGGG
TPSLMNPVIVEGIINKISNLAIIDNQTEITLETNPTSFETEKFKAFKAAGINRISIGVQSLKEDDLKKLGRTHDCMQAIK
TIEAANTIFLRVSFDLIYARSGQTLKDWQEELKQAMQLATSHISLYQLTIEKGTPCYKLFKEGNLILPHSDAAAEMYEWT
NHYLESKKYFRYEISNYAMIGYECLHNLTYWNYNSYLGIGPGAHSRIIESSSSVSAIMMWHKPEKWLDAVKTKNVGIQTN
TNLTHQEIIEEMLMMGLRLSKGINISTLEQKLNTKLENILDMNNLKHYQALDLIRLDENIYLTDKGLMLYSYIVPRLII

Specific function: Unknown

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=293, Percent_Identity=36.1774744027304, Blast_Score=228, Evalue=7e-60,
Organism=Escherichia coli, GI1789325, Length=269, Percent_Identity=39.0334572490706, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082341, Length=189, Percent_Identity=26.4550264550265, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: 1.3.3.3

Molecular weight: Translated: 46063; Mature: 45932

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPKLALAKIQNMRVMANDLSIYIHWPFCLSKCPYCDFNSHVASTIDHNQWLKSYETE
CCCCCCHHHHHHHCCEEEEECEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IEYFKDIIQNKYIKSIFFGGGTPSLMNPVIVEGIINKISNLAIIDNQTEITLETNPTSFE
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCC
TEKFKAFKAAGINRISIGVQSLKEDDLKKLGRTHDCMQAIKTIEAANTIFLRVSFDLIYA
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEEEEEEE
RSGQTLKDWQEELKQAMQLATSHISLYQLTIEKGTPCYKLFKEGNLILPHSDAAAEMYEW
CCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHCCCEEECCCCHHHHHHHH
TNHYLESKKYFRYEISNYAMIGYECLHNLTYWNYNSYLGIGPGAHSRIIESSSSVSAIMM
HHHHHHCCCEEEEEECCEEEEHHHHHHCCEEECCCCEEECCCCCHHHHHCCCCCCEEEEE
WHKPEKWLDAVKTKNVGIQTNTNLTHQEIIEEMLMMGLRLSKGINISTLEQKLNTKLENI
ECCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LDMNNLKHYQALDLIRLDENIYLTDKGLMLYSYIVPRLII
HHHHCCCHHHHHHEEEECCCEEEECCCHHHHHHHHHHHCC
>Mature Secondary Structure 
STKPKLALAKIQNMRVMANDLSIYIHWPFCLSKCPYCDFNSHVASTIDHNQWLKSYETE
CCCCCHHHHHHHCCEEEEECEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IEYFKDIIQNKYIKSIFFGGGTPSLMNPVIVEGIINKISNLAIIDNQTEITLETNPTSFE
HHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCCC
TEKFKAFKAAGINRISIGVQSLKEDDLKKLGRTHDCMQAIKTIEAANTIFLRVSFDLIYA
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEEEEEEE
RSGQTLKDWQEELKQAMQLATSHISLYQLTIEKGTPCYKLFKEGNLILPHSDAAAEMYEW
CCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHCCCEEECCCCHHHHHHHH
TNHYLESKKYFRYEISNYAMIGYECLHNLTYWNYNSYLGIGPGAHSRIIESSSSVSAIMM
HHHHHHCCCEEEEEECCEEEEHHHHHHCCEEECCCCEEECCCCCHHHHHCCCCCCEEEEE
WHKPEKWLDAVKTKNVGIQTNTNLTHQEIIEEMLMMGLRLSKGINISTLEQKLNTKLENI
ECCCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LDMNNLKHYQALDLIRLDENIYLTDKGLMLYSYIVPRLII
HHHHCCCHHHHHHEEEECCCEEEECCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coproporphyrinogen III; O2; H+

Specific reaction: coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]