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The map label for this gene is htrA [H]

Identifier: 229586364

GI number: 229586364

Start: 165846

End: 167324

Strand: Direct

Name: htrA [H]

Synonym: RAF_ORF0155

Alternate gene names: 229586364

Gene position: 165846-167324 (Clockwise)

Preceding gene: 229586363

Following gene: 229586365

Centisome position: 12.97

GC content: 33.2

Gene sequence:

>1479_bases
ATGGTTAATCTGAAAATAGTTATTGTTATAATAGTTTTAATATCAAGTAACGTTGTTCTAGCAAAAGAAAATAGTAGGTC
TTTAAAAGTAGCAGCTCAAGAAGAGAATGAATTTACGGAAATAAATTCTGCTCCTTTAAAAGTAAGTGAAGCCGCTCGTT
ATAGCTTTGCCGATATAGTTGAACCGTTAATTCCTGCAGTCGTTAATATTTCAACAATAGAATATGTTAATAGTAAATCG
GAGAACGCCGAGAAAGATCCTCTGCAAGAAAAAGTTAATGATTTTTTAGAAAAGCTTAATATACCGCTGAATTTGGAAGA
GGTTGATCAAACTCCGAAAAGTGTCCCGCTTGGTTCAGGATTTATTATTGAACCTAACGGCTTAATAGTGACAAACTATC
ATGTAATTGCAAATGTTGATAAAATTAATATAAAACTTGCAGATAATACCGAATTATCGGCTAAATTAATAGGTAACGAT
ACTAAAACCGATTTAGCTCTCTTAAAAATAGATAGCGAGGAACCTCTACCTTTTGTTGAGTTTGGAGATTCAAATGATGC
AAGAGTAGGAGATTGGGTTATTGCGATCGGTAATCCGTTCGGTAATCTAGGTGGTACGGTGACAAGTGGTATTATTTCTT
CTAAAGGGCGGGATATCGATATAGATACGGACAATATAGTCGATAATTTTATTCAAACGGATGCTGCAATTAATAACGGT
AATTCCGGTGGTCCTATGTTTAATTTGGATCAGAAAGTAATCGGCGTAAATACGGCAATTTTTTCACCGCTCGGTACTAA
TATAGGTATCGGTTTTGCAATACCGTCAAATACTGCAAAGCCTATAATCGAACGTCTTAAGAAAGATGGAAAAGTAAGTA
GAGGGCGCCTTGGAGTAACAATACAAGATTTAACCGAGGAAATTTCTGAAGGGCTAGGACTTAAAAATACTAGAGGAGTG
TTAGTAGCTAAAGTACAAGAAGACGGTCCAGGTGATAAAGCGGGAATTAAAACAGGTGATATAATAATAGAGTTTGCAGA
TATACCGGTTAAAAATACTAAAAAATTACGTGTAATTATTGCAGATGCTCCTATTGATCAGGAAGTAAAAGTAAAAATAC
TTCGCGATAAAAAGGAGCTTGAGTTACCTATTAAAATTACTTCGGATAATGAAGAGGTTACCAAAGATTCTACAGAAGAG
ACCAATAAGAAAGAAATAACAAATAAAGAAGAAAATAATTTATCTATTACTAAAAATAATATTACTTTTGGTAATTTGAC
TGAAGAACTAAGACAAAAATATACTATTCCTCAAGATAAAATGGGAATAGTGATAACCAATATTAATCAGAAAAGCATAG
ACGATATAAGTAAGCTAGAAGAATTATATGAAAATGCTAAAAAATCAGATAAGCAAAATATTTTGCTCTTGATTGAAAGG
GGGAGTAGTAATATGTTCGTACCATTGCAGGTTATGTAG

Upstream 100 bases:

>100_bases
AATACTAATTTTGTAATTTCACCGGATGCTGAAGTTTTAAAATATCTAAATCTCACTAAATAGTATACTAGTTTATCTAC
TTTTTATTTACTTGGAGTAT

Downstream 100 bases:

>100_bases
TTTTCTATGTTATTTCCACGAAGGTGGAAATCCAGAAAAATAATCTTAACATTGATACAAAAATTACATATTTAATAAAA
GAAAGCTATAAAAATAAGTT

Product: Periplasmic serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MVNLKIVIVIIVLISSNVVLAKENSRSLKVAAQEENEFTEINSAPLKVSEAARYSFADIVEPLIPAVVNISTIEYVNSKS
ENAEKDPLQEKVNDFLEKLNIPLNLEEVDQTPKSVPLGSGFIIEPNGLIVTNYHVIANVDKINIKLADNTELSAKLIGND
TKTDLALLKIDSEEPLPFVEFGDSNDARVGDWVIAIGNPFGNLGGTVTSGIISSKGRDIDIDTDNIVDNFIQTDAAINNG
NSGGPMFNLDQKVIGVNTAIFSPLGTNIGIGFAIPSNTAKPIIERLKKDGKVSRGRLGVTIQDLTEEISEGLGLKNTRGV
LVAKVQEDGPGDKAGIKTGDIIIEFADIPVKNTKKLRVIIADAPIDQEVKVKILRDKKELELPIKITSDNEEVTKDSTEE
TNKKEITNKEENNLSITKNNITFGNLTEELRQKYTIPQDKMGIVITNINQKSIDDISKLEELYENAKKSDKQNILLLIER
GSSNMFVPLQVM

Sequences:

>Translated_492_residues
MVNLKIVIVIIVLISSNVVLAKENSRSLKVAAQEENEFTEINSAPLKVSEAARYSFADIVEPLIPAVVNISTIEYVNSKS
ENAEKDPLQEKVNDFLEKLNIPLNLEEVDQTPKSVPLGSGFIIEPNGLIVTNYHVIANVDKINIKLADNTELSAKLIGND
TKTDLALLKIDSEEPLPFVEFGDSNDARVGDWVIAIGNPFGNLGGTVTSGIISSKGRDIDIDTDNIVDNFIQTDAAINNG
NSGGPMFNLDQKVIGVNTAIFSPLGTNIGIGFAIPSNTAKPIIERLKKDGKVSRGRLGVTIQDLTEEISEGLGLKNTRGV
LVAKVQEDGPGDKAGIKTGDIIIEFADIPVKNTKKLRVIIADAPIDQEVKVKILRDKKELELPIKITSDNEEVTKDSTEE
TNKKEITNKEENNLSITKNNITFGNLTEELRQKYTIPQDKMGIVITNINQKSIDDISKLEELYENAKKSDKQNILLLIER
GSSNMFVPLQVM
>Mature_492_residues
MVNLKIVIVIIVLISSNVVLAKENSRSLKVAAQEENEFTEINSAPLKVSEAARYSFADIVEPLIPAVVNISTIEYVNSKS
ENAEKDPLQEKVNDFLEKLNIPLNLEEVDQTPKSVPLGSGFIIEPNGLIVTNYHVIANVDKINIKLADNTELSAKLIGND
TKTDLALLKIDSEEPLPFVEFGDSNDARVGDWVIAIGNPFGNLGGTVTSGIISSKGRDIDIDTDNIVDNFIQTDAAINNG
NSGGPMFNLDQKVIGVNTAIFSPLGTNIGIGFAIPSNTAKPIIERLKKDGKVSRGRLGVTIQDLTEEISEGLGLKNTRGV
LVAKVQEDGPGDKAGIKTGDIIIEFADIPVKNTKKLRVIIADAPIDQEVKVKILRDKKELELPIKITSDNEEVTKDSTEE
TNKKEITNKEENNLSITKNNITFGNLTEELRQKYTIPQDKMGIVITNINQKSIDDISKLEELYENAKKSDKQNILLLIER
GSSNMFVPLQVM

Specific function: Serine Protease That Is Required At High Temperature. Involved In The Degradation Of Damaged Proteins. It Can Degrade Icia, Ada, Casein And Globin. Shared Specificity With Degq. [C]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains [H]

Homologues:

Organism=Homo sapiens, GI7019477, Length=298, Percent_Identity=35.9060402684564, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI4506141, Length=306, Percent_Identity=34.9673202614379, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI22129776, Length=378, Percent_Identity=30.6878306878307, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI24308541, Length=281, Percent_Identity=34.1637010676157, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1786356, Length=382, Percent_Identity=36.9109947643979, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1789629, Length=363, Percent_Identity=38.5674931129477, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1789630, Length=273, Percent_Identity=39.1941391941392, Blast_Score=174, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24646839, Length=300, Percent_Identity=34.6666666666667, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.-

Molecular weight: Translated: 53822; Mature: 53822

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLKIVIVIIVLISSNVVLAKENSRSLKVAAQEENEFTEINSAPLKVSEAARYSFADIV
CCEEEEEHEEEEHHCCCEEEEECCCCEEEEEECCCCCCEECCCCCCEEHHHHHCCHHHHH
EPLIPAVVNISTIEYVNSKSENAEKDPLQEKVNDFLEKLNIPLNLEEVDQTPKSVPLGSG
HHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCC
FIIEPNGLIVTNYHVIANVDKINIKLADNTELSAKLIGNDTKTDLALLKIDSEEPLPFVE
EEECCCCEEEEEEEEEEEEEEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCEEE
FGDSNDARVGDWVIAIGNPFGNLGGTVTSGIISSKGRDIDIDTDNIVDNFIQTDAAINNG
ECCCCCCEECEEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCC
NSGGPMFNLDQKVIGVNTAIFSPLGTNIGIGFAIPSNTAKPIIERLKKDGKVSRGRLGVT
CCCCCEEECCCEEEEECHHEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEECEE
IQDLTEEISEGLGLKNTRGVLVAKVQEDGPGDKAGIKTGDIIIEFADIPVKNTKKLRVII
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEECCEEEEEECCCCCCCCEEEEEE
ADAPIDQEVKVKILRDKKELELPIKITSDNEEVTKDSTEETNKKEITNKEENNLSITKNN
ECCCCCCCEEEEEEECCCCEEEEEEEECCCCHHCCCCCCCCCHHHCCCCCCCCEEEEECC
ITFGNLTEELRQKYTIPQDKMGIVITNINQKSIDDISKLEELYENAKKSDKQNILLLIER
CEECHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GSSNMFVPLQVM
CCCCEEEEEEEC
>Mature Secondary Structure
MVNLKIVIVIIVLISSNVVLAKENSRSLKVAAQEENEFTEINSAPLKVSEAARYSFADIV
CCEEEEEHEEEEHHCCCEEEEECCCCEEEEEECCCCCCEECCCCCCEEHHHHHCCHHHHH
EPLIPAVVNISTIEYVNSKSENAEKDPLQEKVNDFLEKLNIPLNLEEVDQTPKSVPLGSG
HHHHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCC
FIIEPNGLIVTNYHVIANVDKINIKLADNTELSAKLIGNDTKTDLALLKIDSEEPLPFVE
EEECCCCEEEEEEEEEEEEEEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCCCCCEEE
FGDSNDARVGDWVIAIGNPFGNLGGTVTSGIISSKGRDIDIDTDNIVDNFIQTDAAINNG
ECCCCCCEECEEEEEECCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCCCCC
NSGGPMFNLDQKVIGVNTAIFSPLGTNIGIGFAIPSNTAKPIIERLKKDGKVSRGRLGVT
CCCCCEEECCCEEEEECHHEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEECEE
IQDLTEEISEGLGLKNTRGVLVAKVQEDGPGDKAGIKTGDIIIEFADIPVKNTKKLRVII
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCEECCEEEEEECCCCCCCCEEEEEE
ADAPIDQEVKVKILRDKKELELPIKITSDNEEVTKDSTEETNKKEITNKEENNLSITKNN
ECCCCCCCEEEEEEECCCCEEEEEEEECCCCHHCCCCCCCCCHHHCCCCCCCCEEEEECC
ITFGNLTEELRQKYTIPQDKMGIVITNINQKSIDDISKLEELYENAKKSDKQNILLLIER
CEECHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
GSSNMFVPLQVM
CCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]