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The map label for this gene is proP1 [H]

Identifier: 229586320

GI number: 229586320

Start: 101972

End: 103228

Strand: Direct

Name: proP1 [H]

Synonym: RAF_ORF0103

Alternate gene names: 229586320

Gene position: 101972-103228 (Clockwise)

Preceding gene: 229586319

Following gene: 229586321

Centisome position: 7.98

GC content: 32.78

Gene sequence:

>1257_bases
ATGAATCGCAGTAAACTAGTATTTTCAAGCGGTATCGCTAACACTTTTGAATGGTATGATTATGTATTATTCGGTTATTT
TGCACCCATAATAGGAACAAAATTTTTTCTAAATGATGATGCTAATACTTCTTTACTGCAGGTTTTTTTAGTATTTGCTA
TAGGTTATTTGGCAAGACCGTTGGGCGGCATATTTTTTGGTGTTATCGGTGATAGATTTGGACGCAAAGTAGCCTTAACT
AGTGCGTTATTTTGCATGTCTGTCCCAACCGTATTAATAGGAATATTACCAACTTACCATACTATCGGTATAACTGCGAC
TATATTAATGATTTGTGTACGAATTCTACAAGGATTATCAATGGGGGGAGTTTTGACCGGTTCTATTTCCTTTGTTATTG
AGCATACTAGCCATAAACACCAAGGCTTCACCGGTAGTATTTCAATGTCTAGTATATGCTCCGGTTTATTGCTTGGTTCT
TTTGTTTCTTATATCGTTAAGAATATCCTAACAGCTTCACAATTTGATAGTTTTGGCTGGAGAATACCTTTTTTACTTGG
TTTTTTTATTCTTTTTGCAGCATTTTATATTAAAAAAAATACACACGAAACACCGAATTTTAAAAATCTAAGAGAGCAGA
AAAAGATTTTACAATCACCGTTAAAAAAAGTCATTACCAATTATTGGTTTGATATATTAATCTCGATTTTTATCAATGCT
CCCGGTTCGATAATATTCTATCTCACTACAATTTACTTGGTATCGTTTTTAAAAATCAGCCGCAACTTTACTGAAAATGA
GGTAAATAGCCTTGCTAGCGTGTGTTACGTGATTATGATAGCCGTAACTTTATTAAGTGGTTATCTATCCGATATTATAG
GTCGCAGGAAAATTTTTGTAATTAATCTGATAATAATTATTGTAACAACGCCATTTTTACTTAATAATTTTGAGAATGGG
GCCTTCACAAGTGTAATTATCTCACAATTGATACTTGCTATACTTGCTGCTAGCTATATCGGTCCTGAACCGGCATTACA
AGCAGAATTGTATCCTACAAATATACGTAACACTGCTCTCTCTATTTCATATAACACTGCTACAAGTATTTTTGGTGGTA
CTACGCCTCTTGTCTTTGAATATTTACTACACAAAACAGGGCATGTAACCTCAGCAGTTTATTATGTCATATTAAGCTGT
ATTTTTGCATTGATTGCCTTATTTTTTTACAAAAATAGAAGTTTAGATAAAATATAA

Upstream 100 bases:

>100_bases
AACGAATTTCCTGATAAAAGCACACTTCATGGAGCAGTGATAATAATTCCTACCACCTTGTTTATAATATATTCCGAAAA
AAAAGCTATGAATAGAAAAC

Downstream 100 bases:

>100_bases
AAAAAATTACAAAACCTACAAACCTAGTTGAATTTAATAACAAAATTGTAGTCTTTAAACCTAAGAGTTATTACTATGGC
GATGCTTGTGAACATAAAAT

Product: Proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGTKFFLNDDANTSLLQVFLVFAIGYLARPLGGIFFGVIGDRFGRKVALT
SALFCMSVPTVLIGILPTYHTIGITATILMICVRILQGLSMGGVLTGSISFVIEHTSHKHQGFTGSISMSSICSGLLLGS
FVSYIVKNILTASQFDSFGWRIPFLLGFFILFAAFYIKKNTHETPNFKNLREQKKILQSPLKKVITNYWFDILISIFINA
PGSIIFYLTTIYLVSFLKISRNFTENEVNSLASVCYVIMIAVTLLSGYLSDIIGRRKIFVINLIIIIVTTPFLLNNFENG
AFTSVIISQLILAILAASYIGPEPALQAELYPTNIRNTALSISYNTATSIFGGTTPLVFEYLLHKTGHVTSAVYYVILSC
IFALIALFFYKNRSLDKI

Sequences:

>Translated_418_residues
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGTKFFLNDDANTSLLQVFLVFAIGYLARPLGGIFFGVIGDRFGRKVALT
SALFCMSVPTVLIGILPTYHTIGITATILMICVRILQGLSMGGVLTGSISFVIEHTSHKHQGFTGSISMSSICSGLLLGS
FVSYIVKNILTASQFDSFGWRIPFLLGFFILFAAFYIKKNTHETPNFKNLREQKKILQSPLKKVITNYWFDILISIFINA
PGSIIFYLTTIYLVSFLKISRNFTENEVNSLASVCYVIMIAVTLLSGYLSDIIGRRKIFVINLIIIIVTTPFLLNNFENG
AFTSVIISQLILAILAASYIGPEPALQAELYPTNIRNTALSISYNTATSIFGGTTPLVFEYLLHKTGHVTSAVYYVILSC
IFALIALFFYKNRSLDKI
>Mature_418_residues
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGTKFFLNDDANTSLLQVFLVFAIGYLARPLGGIFFGVIGDRFGRKVALT
SALFCMSVPTVLIGILPTYHTIGITATILMICVRILQGLSMGGVLTGSISFVIEHTSHKHQGFTGSISMSSICSGLLLGS
FVSYIVKNILTASQFDSFGWRIPFLLGFFILFAAFYIKKNTHETPNFKNLREQKKILQSPLKKVITNYWFDILISIFINA
PGSIIFYLTTIYLVSFLKISRNFTENEVNSLASVCYVIMIAVTLLSGYLSDIIGRRKIFVINLIIIIVTTPFLLNNFENG
AFTSVIISQLILAILAASYIGPEPALQAELYPTNIRNTALSISYNTATSIFGGTTPLVFEYLLHKTGHVTSAVYYVILSC
IFALIALFFYKNRSLDKI

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=412, Percent_Identity=27.9126213592233, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1788292, Length=413, Percent_Identity=27.1186440677966, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1789941, Length=421, Percent_Identity=29.9287410926366, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=30.4878048780488, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082404, Length=400, Percent_Identity=24, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082231, Length=231, Percent_Identity=24.6753246753247, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46468; Mature: 46468

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00189 LIPOYL ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGTKFFLNDDANTSLLQVFLVFAIGYLARP
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHH
LGGIFFGVIGDRFGRKVALTSALFCMSVPTVLIGILPTYHTIGITATILMICVRILQGLS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MGGVLTGSISFVIEHTSHKHQGFTGSISMSSICSGLLLGSFVSYIVKNILTASQFDSFGW
CCCEEECHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIPFLLGFFILFAAFYIKKNTHETPNFKNLREQKKILQSPLKKVITNYWFDILISIFINA
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGSIIFYLTTIYLVSFLKISRNFTENEVNSLASVCYVIMIAVTLLSGYLSDIIGRRKIFV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
INLIIIIVTTPFLLNNFENGAFTSVIISQLILAILAASYIGPEPALQAELYPTNIRNTAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEE
SISYNTATSIFGGTTPLVFEYLLHKTGHVTSAVYYVILSCIFALIALFFYKNRSLDKI
EEEECCHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNRSKLVFSSGIANTFEWYDYVLFGYFAPIIGTKFFLNDDANTSLLQVFLVFAIGYLARP
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHH
LGGIFFGVIGDRFGRKVALTSALFCMSVPTVLIGILPTYHTIGITATILMICVRILQGLS
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
MGGVLTGSISFVIEHTSHKHQGFTGSISMSSICSGLLLGSFVSYIVKNILTASQFDSFGW
CCCEEECHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RIPFLLGFFILFAAFYIKKNTHETPNFKNLREQKKILQSPLKKVITNYWFDILISIFINA
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGSIIFYLTTIYLVSFLKISRNFTENEVNSLASVCYVIMIAVTLLSGYLSDIIGRRKIFV
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
INLIIIIVTTPFLLNNFENGAFTSVIISQLILAILAASYIGPEPALQAELYPTNIRNTAL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCEE
SISYNTATSIFGGTTPLVFEYLLHKTGHVTSAVYYVILSCIFALIALFFYKNRSLDKI
EEEECCHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]