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The map label for this gene is tilS

Identifier: 229586277

GI number: 229586277

Start: 58106

End: 59542

Strand: Direct

Name: tilS

Synonym: RAF_ORF0060

Alternate gene names: 229586277

Gene position: 58106-59542 (Clockwise)

Preceding gene: 229586276

Following gene: 229586278

Centisome position: 4.54

GC content: 30.55

Gene sequence:

>1437_bases
ATGCTATATGAAAAATTTGAGTATAATATCAATAATCTAATAGGTAATTTTGGCTTATCTAAAATATCGATTGCAGTATC
TGGTGGGAGCGATTCGGTAGCACTTCTTTATCTTGCTAATATTTGGGCAGAGAAAAATAATATAGAATTATTTGTTATAT
CGGTTGATCATAACTTACGGGAACAGTCGAAGCAAGAGACCCATTATATCCAAAATATCAGTAATAGTCTAAATCGCAAG
CATTATAGTTTATCTTTTTACCATCAAAATAATTTTTCCAATTTACAAGAAAGAGCAAGAGAAGGACGCTATGATTTGAT
GACAAATCTATGTCTAGAACTCGATATATTAGTACTTTTAACTGCTCACCATGAAGATGATTACGTAGAAAATTTTTGTT
TAAGATTAGAGCGTAATAGCGGTATATTTGGACTTAGTAGCAGTAATATCAATTGGTATAATAATATACAAATAATTAGA
CCGCTATATAATATTCCAAAAAGTAAATTAGTAGAATATTTAGTCAGGCATAATATAAAATGGTTTGAAGATGAGTCAAA
TTCATCTGATAAATATAGACGAAATGTTATTAGGCAAAAATTAGCTAAAGGTGCAGATTATATTAGACATTTTTCCAAAC
CAGTTTATAGAGAGGAATTTAAAGGAGACACGGAACGCAGCACCGCAGCGTACACTTTAGTACGTGAGGATGCGAGTACC
GGAACGGCGTCTAAATTATCTCTAGAAGCGAAGTGTGGAAAGATGTCTAAAGCCGCAATAATCTCACAACAACTTAAAAC
CAACAAACTTATAGAAAACGAGTTTAAGCCTGAATTAATATCGGCGATAGCTGAAGCGGTCAAGATTTTTGAATATGGCT
TTGCTTTTCTTGATTTAGTTAAATTTGATAAGTTTTCAAATGAGGTGAAAGTACAAATAATTAATTTTTTACTGATAATA
ATTAGTGGACAATCTAGAGTAGCTCGTTTTTATTCGGTAGAACCTATTTTGAAATTAATTACTCAAGACGTAAACTTTAA
GAATACACTTCATGGCTGTATAATTAAGCGCATACAAAACGAGTTACTTATATACAGGGAATTCGGCAAAAAATTGCCTG
AGAGTAAAATATTACTAGATAAATCCGTTATTTGGGATAATAGATTTTGTATCACAAAAAATCAAGAAACTCCAAATTGT
TTTGTAACTCATCTATCGTTAAAAGATTATAAAATAATAAAGAAACAATTAGATTTAGAACCTTTGAAAAATCTATCCTG
CAAAAACCACAATGCAGTTTTATTGACCTTACCTATCATTAAAATACTTGAAAAAGTTATAGCAATACCACATATATCAT
ATTATGATAACGACATGTGGAACTTTGAAGTATCTTTTTCTCCAAATTTTGTATCTCGTTTCACCCATTTTTGCTAA

Upstream 100 bases:

>100_bases
CATAAGAGCCATGCAACAACGTCCCGCCTACGCGGAAATGACATACTACTAAGTTTGACCACAATATCCAAACACTTTAC
AATACTAACATTTTTCAACC

Downstream 100 bases:

>100_bases
CACCGGTATTTAAGAGGTTATCTGTAAATAAATTTTAACGATAAAATTTATTTACAGACCTCTCTTATACCAATAAAAAT
TTTTATTAGGTTTTATATCG

Product: mesJ; tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLYEKFEYNINNLIGNFGLSKISIAVSGGSDSVALLYLANIWAEKNNIELFVISVDHNLREQSKQETHYIQNISNSLNRK
HYSLSFYHQNNFSNLQERAREGRYDLMTNLCLELDILVLLTAHHEDDYVENFCLRLERNSGIFGLSSSNINWYNNIQIIR
PLYNIPKSKLVEYLVRHNIKWFEDESNSSDKYRRNVIRQKLAKGADYIRHFSKPVYREEFKGDTERSTAAYTLVREDAST
GTASKLSLEAKCGKMSKAAIISQQLKTNKLIENEFKPELISAIAEAVKIFEYGFAFLDLVKFDKFSNEVKVQIINFLLII
ISGQSRVARFYSVEPILKLITQDVNFKNTLHGCIIKRIQNELLIYREFGKKLPESKILLDKSVIWDNRFCITKNQETPNC
FVTHLSLKDYKIIKKQLDLEPLKNLSCKNHNAVLLTLPIIKILEKVIAIPHISYYDNDMWNFEVSFSPNFVSRFTHFC

Sequences:

>Translated_478_residues
MLYEKFEYNINNLIGNFGLSKISIAVSGGSDSVALLYLANIWAEKNNIELFVISVDHNLREQSKQETHYIQNISNSLNRK
HYSLSFYHQNNFSNLQERAREGRYDLMTNLCLELDILVLLTAHHEDDYVENFCLRLERNSGIFGLSSSNINWYNNIQIIR
PLYNIPKSKLVEYLVRHNIKWFEDESNSSDKYRRNVIRQKLAKGADYIRHFSKPVYREEFKGDTERSTAAYTLVREDAST
GTASKLSLEAKCGKMSKAAIISQQLKTNKLIENEFKPELISAIAEAVKIFEYGFAFLDLVKFDKFSNEVKVQIINFLLII
ISGQSRVARFYSVEPILKLITQDVNFKNTLHGCIIKRIQNELLIYREFGKKLPESKILLDKSVIWDNRFCITKNQETPNC
FVTHLSLKDYKIIKKQLDLEPLKNLSCKNHNAVLLTLPIIKILEKVIAIPHISYYDNDMWNFEVSFSPNFVSRFTHFC
>Mature_478_residues
MLYEKFEYNINNLIGNFGLSKISIAVSGGSDSVALLYLANIWAEKNNIELFVISVDHNLREQSKQETHYIQNISNSLNRK
HYSLSFYHQNNFSNLQERAREGRYDLMTNLCLELDILVLLTAHHEDDYVENFCLRLERNSGIFGLSSSNINWYNNIQIIR
PLYNIPKSKLVEYLVRHNIKWFEDESNSSDKYRRNVIRQKLAKGADYIRHFSKPVYREEFKGDTERSTAAYTLVREDAST
GTASKLSLEAKCGKMSKAAIISQQLKTNKLIENEFKPELISAIAEAVKIFEYGFAFLDLVKFDKFSNEVKVQIINFLLII
ISGQSRVARFYSVEPILKLITQDVNFKNTLHGCIIKRIQNELLIYREFGKKLPESKILLDKSVIWDNRFCITKNQETPNC
FVTHLSLKDYKIIKKQLDLEPLKNLSCKNHNAVLLTLPIIKILEKVIAIPHISYYDNDMWNFEVSFSPNFVSRFTHFC

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=180, Percent_Identity=31.1111111111111, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_RICAE (C3PM75)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002844778.1
- GeneID:   7815903
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF0060
- OMA:   NIQIIRP
- ProtClustDB:   CLSK870716
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR005728
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432
- TIGRFAMs:   TIGR01045

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 55622; Mature: 55622

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYEKFEYNINNLIGNFGLSKISIAVSGGSDSVALLYLANIWAEKNNIELFVISVDHNLR
CCCCHHHCCHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHHCCCCCEEEEEEEECCCHH
EQSKQETHYIQNISNSLNRKHYSLSFYHQNNFSNLQERAREGRYDLMTNLCLELDILVLL
HHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHEEEEEE
TAHHEDDYVENFCLRLERNSGIFGLSSSNINWYNNIQIIRPLYNIPKSKLVEYLVRHNIK
EECCCHHHHHHHHHEEECCCCEEEEECCCCCEECCEEEEEHHHHCCHHHHHHHHHHCCCE
WFEDESNSSDKYRRNVIRQKLAKGADYIRHFSKPVYREEFKGDTERSTAAYTLVREDAST
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHEEEEEECCCCC
GTASKLSLEAKCGKMSKAAIISQQLKTNKLIENEFKPELISAIAEAVKIFEYGFAFLDLV
CCCHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KFDKFSNEVKVQIINFLLIIISGQSRVARFYSVEPILKLITQDVNFKNTLHGCIIKRIQN
HHHHCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELLIYREFGKKLPESKILLDKSVIWDNRFCITKNQETPNCFVTHLSLKDYKIIKKQLDLE
HHHHHHHHHHHCCCCCEEEEHHEEECCEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCC
PLKNLSCKNHNAVLLTLPIIKILEKVIAIPHISYYDNDMWNFEVSFSPNFVSRFTHFC
HHHCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEECCCCCEEEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MLYEKFEYNINNLIGNFGLSKISIAVSGGSDSVALLYLANIWAEKNNIELFVISVDHNLR
CCCCHHHCCHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHHCCCCCEEEEEEEECCCHH
EQSKQETHYIQNISNSLNRKHYSLSFYHQNNFSNLQERAREGRYDLMTNLCLELDILVLL
HHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHEEEEEE
TAHHEDDYVENFCLRLERNSGIFGLSSSNINWYNNIQIIRPLYNIPKSKLVEYLVRHNIK
EECCCHHHHHHHHHEEECCCCEEEEECCCCCEECCEEEEEHHHHCCHHHHHHHHHHCCCE
WFEDESNSSDKYRRNVIRQKLAKGADYIRHFSKPVYREEFKGDTERSTAAYTLVREDAST
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHEEEEEECCCCC
GTASKLSLEAKCGKMSKAAIISQQLKTNKLIENEFKPELISAIAEAVKIFEYGFAFLDLV
CCCHHEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
KFDKFSNEVKVQIINFLLIIISGQSRVARFYSVEPILKLITQDVNFKNTLHGCIIKRIQN
HHHHCCCHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ELLIYREFGKKLPESKILLDKSVIWDNRFCITKNQETPNCFVTHLSLKDYKIIKKQLDLE
HHHHHHHHHHHCCCCCEEEEHHEEECCEEEEECCCCCCCEEEEEECCHHHHHHHHHCCCC
PLKNLSCKNHNAVLLTLPIIKILEKVIAIPHISYYDNDMWNFEVSFSPNFVSRFTHFC
HHHCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEECCCCCEEEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA