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The map label for this gene is gspE [C]

Identifier: 227828861

GI number: 227828861

Start: 2450838

End: 2452430

Strand: Direct

Name: gspE [C]

Synonym: M1425_2634

Alternate gene names: 227828861

Gene position: 2450838-2452430 (Clockwise)

Preceding gene: 227828860

Following gene: 227828862

Centisome position: 93.94

GC content: 34.84

Gene sequence:

>1593_bases
GTGACTACAATAAAGTATTTTATTTTTATTTTTTTAAATAGGTATATGGTACTTATTGGTAAAAAGAAATCACATAATAA
ATTTGAAGAATTATCCTATTATCTACAATCGGTTGATGAATCGGGAATAATGTCTCTTAGAGCTAACTTGGAAAATCACA
ATATCGTAGAGCACTATTCTCTAAAAAACTTTGACGTTGAAGTTTACATTGTCGAAAAAGAGGGGATTGGTTACTATTTA
GTAAACGAACCACAGCTTGATCAGAGAGAAGAAAAGATCTTATTAGCGATTCTTGACGGTTTAATATATTCTCCCACAAC
TGTGGTTTCAAATAAACAAATTGACGTGGAAAAACTACAAGAAGATGTATTGAAAATTTCAGCTAAATTGGGAGTAATAG
GTGACGTAAAGAGAAACTTAAAGAAATATATGTACTATATAACTCGAGAGATAAAATATTCAGTCCTACAAGTACCAATG
AGCGACCCTTTCATCGAAGAGATAGAACTAGTATCCCCAACGACACCAATTTCAGTAGTCCATAGCAGACATAGTGAATG
GCCTAGATTGGAGACTAATATCATTTTAGGGAGTGAGTTGAAGGTTAGGAGAATAGTAGAGAGGTTAGCATCTTTAGGTG
GGAGAAGCGTAAGTACAGCTACACCATTACAAGATTTTATGTTACACGAAGGTCATAGAGTTGCAGTGAGTTACGGTGAG
GAAATAAGTAGAGGAACGACATTTAATATAAGAAAATTCCCAGAAAAGCCTCTAACGATTATTGACTTGGTTTACAAGTA
TGGTACATTAAGCGAGCTAATGGCAGTTTACTTATGGATAATTGCCGAGGCTAAGCTATTTACATTTCTCGTAGGCCCAA
CTGGTAGCGGAAAGACCACTGCATTAAACTCACTATTAATGCTACTTAATCCATTAGCGAAATATTTAACGATTGAGGAT
ACACCGGAGCTAAAATTACCTCACAAATATTGGATACAATTCTATACTAGACCTTCAAACTATGAAGGGAGTAAGGATAT
CAGTTATTATGAACTAGTCAGACTCTCCTTAAGATATAGACCAGATTACATTATAGTAGGGGAAGTAAGAGGGAAAGAAA
TAGAATGGCTAGTTCAAGCTGTTGCCAGTGGTCATGGTGGGCTCACAACATTTCATGGGTCTAATCACGTTGACTTAATA
ACTAGAATTAGTGGGTTATTGGGTCAAGATCTATCGTTACAATTTAAACAGCTAATTTCAGTGATTGCAATCATAAAGAG
AATTGAAACCGAGAATAGAAAGATGAATAGGAAAATAATATCTATTGTTGAAAATGATGGAAACGGATTTAGGGAAGTAT
TTAAATATGATTATGAAAGGAAGAGCTTCATTCCCAATAATTCAAAAGAAATTGGTAGCATACAGCTGGAAAAAGCTAGA
GAATTATTAGGCTGGAGTAAGGATAAGTTGTATAAGGAGATAGAAAATAGACTGTTATTAATAAGGGGGCTTGCTGAAAG
GGGAGTTTACGACTACGACGCATTGGCAAAAGAGTTAGTTAAATATTATATAAATGGTGATAGGGGTGGCTGA

Upstream 100 bases:

>100_bases
CTGTACCTAAAGCAATTGAAGGAAATAATTACGTTATTACTGTGATTGTTAAGTACAATAACGGACAAACCTATGCTACA
TCGGTAGAAGTTATTGATGA

Downstream 100 bases:

>100_bases
AAGAAGAATTAAATTGGCTAGCATAATCTCATATGATTTCTTGTTTCTAGTTTTACTAGCGGGTTTACTAGATTTAATCA
TTACGTTATTTAGAGAACGA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTTIKYFIFIFLNRYMVLIGKKKSHNKFEELSYYLQSVDESGIMSLRANLENHNIVEHYSLKNFDVEVYIVEKEGIGYYL
VNEPQLDQREEKILLAILDGLIYSPTTVVSNKQIDVEKLQEDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSVLQVPM
SDPFIEEIELVSPTTPISVVHSRHSEWPRLETNIILGSELKVRRIVERLASLGGRSVSTATPLQDFMLHEGHRVAVSYGE
EISRGTTFNIRKFPEKPLTIIDLVYKYGTLSELMAVYLWIIAEAKLFTFLVGPTGSGKTTALNSLLMLLNPLAKYLTIED
TPELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHVDLI
TRISGLLGQDLSLQFKQLISVIAIIKRIETENRKMNRKIISIVENDGNGFREVFKYDYERKSFIPNNSKEIGSIQLEKAR
ELLGWSKDKLYKEIENRLLLIRGLAERGVYDYDALAKELVKYYINGDRGG

Sequences:

>Translated_530_residues
MTTIKYFIFIFLNRYMVLIGKKKSHNKFEELSYYLQSVDESGIMSLRANLENHNIVEHYSLKNFDVEVYIVEKEGIGYYL
VNEPQLDQREEKILLAILDGLIYSPTTVVSNKQIDVEKLQEDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSVLQVPM
SDPFIEEIELVSPTTPISVVHSRHSEWPRLETNIILGSELKVRRIVERLASLGGRSVSTATPLQDFMLHEGHRVAVSYGE
EISRGTTFNIRKFPEKPLTIIDLVYKYGTLSELMAVYLWIIAEAKLFTFLVGPTGSGKTTALNSLLMLLNPLAKYLTIED
TPELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHVDLI
TRISGLLGQDLSLQFKQLISVIAIIKRIETENRKMNRKIISIVENDGNGFREVFKYDYERKSFIPNNSKEIGSIQLEKAR
ELLGWSKDKLYKEIENRLLLIRGLAERGVYDYDALAKELVKYYINGDRGG
>Mature_529_residues
TTIKYFIFIFLNRYMVLIGKKKSHNKFEELSYYLQSVDESGIMSLRANLENHNIVEHYSLKNFDVEVYIVEKEGIGYYLV
NEPQLDQREEKILLAILDGLIYSPTTVVSNKQIDVEKLQEDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSVLQVPMS
DPFIEEIELVSPTTPISVVHSRHSEWPRLETNIILGSELKVRRIVERLASLGGRSVSTATPLQDFMLHEGHRVAVSYGEE
ISRGTTFNIRKFPEKPLTIIDLVYKYGTLSELMAVYLWIIAEAKLFTFLVGPTGSGKTTALNSLLMLLNPLAKYLTIEDT
PELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYRPDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHVDLIT
RISGLLGQDLSLQFKQLISVIAIIKRIETENRKMNRKIISIVENDGNGFREVFKYDYERKSFIPNNSKEIGSIQLEKARE
LLGWSKDKLYKEIENRLLLIRGLAERGVYDYDALAKELVKYYINGDRGG

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=150, Percent_Identity=34, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 61040; Mature: 60909

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKYFIFIFLNRYMVLIGKKKSHNKFEELSYYLQSVDESGIMSLRANLENHNIVEHYS
CCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEC
LKNFDVEVYIVEKEGIGYYLVNEPQLDQREEKILLAILDGLIYSPTTVVSNKQIDVEKLQ
CCCCCEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHH
EDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSVLQVPMSDPFIEEIELVSPTTPISVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCHHHHEECCCCCCHHHH
HSRHSEWPRLETNIILGSELKVRRIVERLASLGGRSVSTATPLQDFMLHEGHRVAVSYGE
HHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCC
EISRGTTFNIRKFPEKPLTIIDLVYKYGTLSELMAVYLWIIAEAKLFTFLVGPTGSGKTT
HHCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHH
ALNSLLMLLNPLAKYLTIEDTPELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYR
HHHHHHHHHHHHHHHEEECCCCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHEEC
PDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHVDLITRISGLLGQDLSLQFKQLIS
CCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHH
VIAIIKRIETENRKMNRKIISIVENDGNGFREVFKYDYERKSFIPNNSKEIGSIQLEKAR
HHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCHHCCCCCCCCCCCCEEHHHHH
ELLGWSKDKLYKEIENRLLLIRGLAERGVYDYDALAKELVKYYINGDRGG
HHHCCCHHHHHHHHHHHEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTIKYFIFIFLNRYMVLIGKKKSHNKFEELSYYLQSVDESGIMSLRANLENHNIVEHYS
CHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEEC
LKNFDVEVYIVEKEGIGYYLVNEPQLDQREEKILLAILDGLIYSPTTVVSNKQIDVEKLQ
CCCCCEEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHH
EDVLKISAKLGVIGDVKRNLKKYMYYITREIKYSVLQVPMSDPFIEEIELVSPTTPISVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEHEEECCCCCCCHHHHEECCCCCCHHHH
HSRHSEWPRLETNIILGSELKVRRIVERLASLGGRSVSTATPLQDFMLHEGHRVAVSYGE
HHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCEEEEECCC
EISRGTTFNIRKFPEKPLTIIDLVYKYGTLSELMAVYLWIIAEAKLFTFLVGPTGSGKTT
HHCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCHH
ALNSLLMLLNPLAKYLTIEDTPELKLPHKYWIQFYTRPSNYEGSKDISYYELVRLSLRYR
HHHHHHHHHHHHHHHEEECCCCCCCCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHEEC
PDYIIVGEVRGKEIEWLVQAVASGHGGLTTFHGSNHVDLITRISGLLGQDLSLQFKQLIS
CCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHH
VIAIIKRIETENRKMNRKIISIVENDGNGFREVFKYDYERKSFIPNNSKEIGSIQLEKAR
HHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHCCCHHCCCCCCCCCCCCEEHHHHH
ELLGWSKDKLYKEIENRLLLIRGLAERGVYDYDALAKELVKYYINGDRGG
HHHCCCHHHHHHHHHHHEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]