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The map label for this gene is cysI [H]

Identifier: 227828661

GI number: 227828661

Start: 2244465

End: 2246360

Strand: Direct

Name: cysI [H]

Synonym: M1425_2418

Alternate gene names: 227828661

Gene position: 2244465-2246360 (Clockwise)

Preceding gene: 227828660

Following gene: 227828662

Centisome position: 86.03

GC content: 37.34

Gene sequence:

>1896_bases
GTGAGGATTCCCATAGAGCCCAATTTTAGAACAGATCCTAAGGATTACAGTGAGGAAGAGAGAGTAAAGCTGAAAACGAA
GGGATTAACTGAGGATACCATAGGGATATATTCATCATTTCACGATTTTACTAGGGACGACCTCGAGAAGGAAGTATCTT
TGATAGCTAAAAGTTTTGGAATTTATATGGAATTCAATAGGGATAAGATGAAATCAAACGCAATAAAAGACTGGATCTAC
ATGGTAAGGTTACCGATTCCAGGAGGAGGTCCAATAAGACTTGACCAATGGAGAATTTTAGATGATGTTTCAAATAAGTA
TACCATATCAGATGCATATACTGGATATCCTTTACCTTCTCTGAAACTAACCACGAGACAAGCTATTCAATTTCACCATG
TAAAGAAAAAGGATTTGCCTAACCTTATAAGGGAGATTGCAGAATCTGGCTTTCTTACCATAAATGGCTGTGGGGATAAT
TTAAGGAATACGATAGCTTGTCCACTTTCCAAGTTTTGGATTTTCGACTCAAATACGCTCGCTAGAAAAATTGCTGACTA
CTTTAGATTACCTTCAGAGTTGTATCTTCAAGTTTTTGAAATACCTCTTTCTGAAGAAAGGCAATTTAATAATAAAGAAT
CCTTCAAATATGCTGATAATCTTCTACCCAGAAAGTTTAAGATTGGAATAGCTGGAGTAATGAGGACAATTGACGGAAAG
TACATAATTGACAATTGCGTTGAGGTGCGAGCTAACGATATAGGCATAGTCCCTCTAATAGAAGGGGAAAAGGTAATAGG
TTATCAGATATACGTTGGTGGATCAATGGGTGAAAATAACTCCTATCCCACTTTTTCAGCCCTCGGACTTCCAATTGGGA
CTGTATCTAAAGACGAGGAGTTATTGAGAGTATTGGATGCAATTGTGAGAATACAAGAGCAATGGGGAGATAGGAAGAAT
AGGCATTGGGCTAGGTTTAAGTACGTTGTATATAAAATGGGATTAGAGTGGGTAAGGGAAAAAGTATGGGAATACTCAAG
GATTCGATTAGGTAAAATAGTGAATGTAAACTTAGATCATAGTGACTTACACTTGGGATGGATAGACTTAGGTAATAGCA
AGTGGGCTTATGGTGTTTTCGTTGAAGACGGTAGACTAATAGATGGAAAAAACGGTAAGGTAAAGAGTATGATCAGATAC
ATTGCGGATAATTTCCATAATGTAACGTTTTACATAACTCCAAATCAACACTTATTAATTACGGAAATAAACGAGGATCA
GAGGGAGGAAATTGAGAAAACACTAAGGGATTTCAACTACGGCTTTAGAGATGGAAAACCTTACTCGAATTTAAGAACGA
ATTCGATTGCATGTGTAGGATTTCCAACATGTAAATTATCATTTACAGATTCCGAAAGATTCCTTCCGAGTCTAATTGAC
GAGTTGGAGAGAAGAGGATGGAAAGATATTCCAGTTTCAATAGGACTATCGGGATGTGTCGCTCAATGTTCGAGACCAGC
CGTTTATCCCATTAGTTGGATTGGTAGTGGATATGAGCTCTATATGTTAAAGATAGGTGGAGGAAATGGTAATTTGGGAG
AGCCGCTAATTGACTGGGATGAAAATGTAATTTACCTATATCAAGTTCCAGCCAATAGATTAGCAGACGTGACTGAAGCG
TTGTTTGAATTATACGAGAGAAATAAGGATATAAGTGAGGAACCAGGAAAAGTATTCAGAGTAATAGGTAATAGGAAGAT
AATTGAATGGTTAAAGAGCCATGAGAAGACTAGAGACCTAATGAGACCTCATAAGTTTGATAAAAAGATCGAGGGTTATA
GGGAATATCACGACTTGTTGAGAAAGCGTTTGGAAGAGGTGAATAGGTATAGGTAA

Upstream 100 bases:

>100_bases
GTACTAGACCAGTAAAACCGTGGGAGCATCCAAGAGCTGGTAGATGGTGGTGGGAGCAGAATAGTGACAAGGAGTGTGGA
CTTCACTATAGAGGGGTGAA

Downstream 100 bases:

>100_bases
GGTAGTTTTAGTGGGAGCTGGTCCTGGGGATCCCGAACTAATAACGTTAAAGGGTATTAAATACTTACAAATGGCTGATG
TAATAGTTTACGATAAGTTA

Product: nitrite and sulphite reductase 4Fe-4S region

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MRIPIEPNFRTDPKDYSEEERVKLKTKGLTEDTIGIYSSFHDFTRDDLEKEVSLIAKSFGIYMEFNRDKMKSNAIKDWIY
MVRLPIPGGGPIRLDQWRILDDVSNKYTISDAYTGYPLPSLKLTTRQAIQFHHVKKKDLPNLIREIAESGFLTINGCGDN
LRNTIACPLSKFWIFDSNTLARKIADYFRLPSELYLQVFEIPLSEERQFNNKESFKYADNLLPRKFKIGIAGVMRTIDGK
YIIDNCVEVRANDIGIVPLIEGEKVIGYQIYVGGSMGENNSYPTFSALGLPIGTVSKDEELLRVLDAIVRIQEQWGDRKN
RHWARFKYVVYKMGLEWVREKVWEYSRIRLGKIVNVNLDHSDLHLGWIDLGNSKWAYGVFVEDGRLIDGKNGKVKSMIRY
IADNFHNVTFYITPNQHLLITEINEDQREEIEKTLRDFNYGFRDGKPYSNLRTNSIACVGFPTCKLSFTDSERFLPSLID
ELERRGWKDIPVSIGLSGCVAQCSRPAVYPISWIGSGYELYMLKIGGGNGNLGEPLIDWDENVIYLYQVPANRLADVTEA
LFELYERNKDISEEPGKVFRVIGNRKIIEWLKSHEKTRDLMRPHKFDKKIEGYREYHDLLRKRLEEVNRYR

Sequences:

>Translated_631_residues
MRIPIEPNFRTDPKDYSEEERVKLKTKGLTEDTIGIYSSFHDFTRDDLEKEVSLIAKSFGIYMEFNRDKMKSNAIKDWIY
MVRLPIPGGGPIRLDQWRILDDVSNKYTISDAYTGYPLPSLKLTTRQAIQFHHVKKKDLPNLIREIAESGFLTINGCGDN
LRNTIACPLSKFWIFDSNTLARKIADYFRLPSELYLQVFEIPLSEERQFNNKESFKYADNLLPRKFKIGIAGVMRTIDGK
YIIDNCVEVRANDIGIVPLIEGEKVIGYQIYVGGSMGENNSYPTFSALGLPIGTVSKDEELLRVLDAIVRIQEQWGDRKN
RHWARFKYVVYKMGLEWVREKVWEYSRIRLGKIVNVNLDHSDLHLGWIDLGNSKWAYGVFVEDGRLIDGKNGKVKSMIRY
IADNFHNVTFYITPNQHLLITEINEDQREEIEKTLRDFNYGFRDGKPYSNLRTNSIACVGFPTCKLSFTDSERFLPSLID
ELERRGWKDIPVSIGLSGCVAQCSRPAVYPISWIGSGYELYMLKIGGGNGNLGEPLIDWDENVIYLYQVPANRLADVTEA
LFELYERNKDISEEPGKVFRVIGNRKIIEWLKSHEKTRDLMRPHKFDKKIEGYREYHDLLRKRLEEVNRYR
>Mature_631_residues
MRIPIEPNFRTDPKDYSEEERVKLKTKGLTEDTIGIYSSFHDFTRDDLEKEVSLIAKSFGIYMEFNRDKMKSNAIKDWIY
MVRLPIPGGGPIRLDQWRILDDVSNKYTISDAYTGYPLPSLKLTTRQAIQFHHVKKKDLPNLIREIAESGFLTINGCGDN
LRNTIACPLSKFWIFDSNTLARKIADYFRLPSELYLQVFEIPLSEERQFNNKESFKYADNLLPRKFKIGIAGVMRTIDGK
YIIDNCVEVRANDIGIVPLIEGEKVIGYQIYVGGSMGENNSYPTFSALGLPIGTVSKDEELLRVLDAIVRIQEQWGDRKN
RHWARFKYVVYKMGLEWVREKVWEYSRIRLGKIVNVNLDHSDLHLGWIDLGNSKWAYGVFVEDGRLIDGKNGKVKSMIRY
IADNFHNVTFYITPNQHLLITEINEDQREEIEKTLRDFNYGFRDGKPYSNLRTNSIACVGFPTCKLSFTDSERFLPSLID
ELERRGWKDIPVSIGLSGCVAQCSRPAVYPISWIGSGYELYMLKIGGGNGNLGEPLIDWDENVIYLYQVPANRLADVTEA
LFELYERNKDISEEPGKVFRVIGNRKIIEWLKSHEKTRDLMRPHKFDKKIEGYREYHDLLRKRLEEVNRYR

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=485, Percent_Identity=26.8041237113402, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6322597, Length=563, Percent_Identity=30.3730017761989, Blast_Score=197, Evalue=4e-51,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 73312; Mature: 73312

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPIEPNFRTDPKDYSEEERVKLKTKGLTEDTIGIYSSFHDFTRDDLEKEVSLIAKSFG
CCCCCCCCCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IYMEFNRDKMKSNAIKDWIYMVRLPIPGGGPIRLDQWRILDDVSNKYTISDAYTGYPLPS
EEEEECHHHHHHHHHHEEEEEEEEECCCCCCEEECCEEEHHHCCCCEEECCCCCCCCCCC
LKLTTRQAIQFHHVKKKDLPNLIREIAESGFLTINGCGDNLRNTIACPLSKFWIFDSNTL
EEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHEECCCEEEEEECCCHH
ARKIADYFRLPSELYLQVFEIPLSEERQFNNKESFKYADNLLPRKFKIGIAGVMRTIDGK
HHHHHHHHHCCHHHHHHHHCCCCCHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCCC
YIIDNCVEVRANDIGIVPLIEGEKVIGYQIYVGGSMGENNSYPTFSALGLPIGTVSKDEE
EEECHHHHHCCCCEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
LLRVLDAIVRIQEQWGDRKNRHWARFKYVVYKMGLEWVREKVWEYSRIRLGKIVNVNLDH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEEECCC
SDLHLGWIDLGNSKWAYGVFVEDGRLIDGKNGKVKSMIRYIADNFHNVTFYITPNQHLLI
CCEEEEEEEECCCCEEEEEEEECCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCEEEE
TEINEDQREEIEKTLRDFNYGFRDGKPYSNLRTNSIACVGFPTCKLSFTDSERFLPSLID
EECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHH
ELERRGWKDIPVSIGLSGCVAQCSRPAVYPISWIGSGYELYMLKIGGGNGNLGEPLIDWD
HHHHCCCCCCCEECCCHHHHHHCCCCCEEEHHCCCCCEEEEEEEEECCCCCCCCCCCCCC
ENVIYLYQVPANRLADVTEALFELYERNKDISEEPGKVFRVIGNRKIIEWLKSHEKTRDL
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
MRPHKFDKKIEGYREYHDLLRKRLEEVNRYR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRIPIEPNFRTDPKDYSEEERVKLKTKGLTEDTIGIYSSFHDFTRDDLEKEVSLIAKSFG
CCCCCCCCCCCCCCCCCCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IYMEFNRDKMKSNAIKDWIYMVRLPIPGGGPIRLDQWRILDDVSNKYTISDAYTGYPLPS
EEEEECHHHHHHHHHHEEEEEEEEECCCCCCEEECCEEEHHHCCCCEEECCCCCCCCCCC
LKLTTRQAIQFHHVKKKDLPNLIREIAESGFLTINGCGDNLRNTIACPLSKFWIFDSNTL
EEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHEECCCEEEEEECCCHH
ARKIADYFRLPSELYLQVFEIPLSEERQFNNKESFKYADNLLPRKFKIGIAGVMRTIDGK
HHHHHHHHHCCHHHHHHHHCCCCCHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHHCCCC
YIIDNCVEVRANDIGIVPLIEGEKVIGYQIYVGGSMGENNSYPTFSALGLPIGTVSKDEE
EEECHHHHHCCCCEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHH
LLRVLDAIVRIQEQWGDRKNRHWARFKYVVYKMGLEWVREKVWEYSRIRLGKIVNVNLDH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEEECCC
SDLHLGWIDLGNSKWAYGVFVEDGRLIDGKNGKVKSMIRYIADNFHNVTFYITPNQHLLI
CCEEEEEEEECCCCEEEEEEEECCEEEECCCCHHHHHHHHHHCCCCCEEEEECCCCEEEE
TEINEDQREEIEKTLRDFNYGFRDGKPYSNLRTNSIACVGFPTCKLSFTDSERFLPSLID
EECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCEEEEEECCCHHHHHHHHH
ELERRGWKDIPVSIGLSGCVAQCSRPAVYPISWIGSGYELYMLKIGGGNGNLGEPLIDWD
HHHHCCCCCCCEECCCHHHHHHCCCCCEEEHHCCCCCEEEEEEEEECCCCCCCCCCCCCC
ENVIYLYQVPANRLADVTEALFELYERNKDISEEPGKVFRVIGNRKIIEWLKSHEKTRDL
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
MRPHKFDKKIEGYREYHDLLRKRLEEVNRYR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA