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The map label for this gene is cysG1 [H]

Identifier: 227828662

GI number: 227828662

Start: 2246371

End: 2247045

Strand: Direct

Name: cysG1 [H]

Synonym: M1425_2419

Alternate gene names: 227828662

Gene position: 2246371-2247045 (Clockwise)

Preceding gene: 227828661

Following gene: 227828663

Centisome position: 86.11

GC content: 37.63

Gene sequence:

>675_bases
GTGGGAGCTGGTCCTGGGGATCCCGAACTAATAACGTTAAAGGGTATTAAATACTTACAAATGGCTGATGTAATAGTTTA
CGATAAGTTAGTATCTAGAGAGCTAATTAGTTACGCTAAGCCGTCTTGTAGTGTTATACTCCTTAGTAGTGACGATATTG
AAATTGACTTATTGAAGAAGTATGCTTTAGAAAATAAACTGGTGATAAGGTTAAAAAATGGGGATCCTTACGTGTTCGGA
AGGGGAGGGAAAATTTGCCAAGAACTAATTAAGGACGGTATAGAGTGTGAAGTGGTACCGGGAGTTTCATCAGTTAACTC
AGTTCCAGCATATGCTGGAATACCGCTTACTTTCAACGGTATCTCTGATATGATAACAGTTATAAGTGCTGTTACGCAAG
GTGGTAGGCTCTTTGACTTTAAAAAAATTCCAGATGATGGTACTTTAGTAGTTTTAATGAGCGGTAGGAGGTTGGAGGAG
GTAAGTAAAGAACTAATGACAAAGAGGGATCCTTCTGAAGAGGTTGCAGTTATACAAAGAGGAACTTATTTTGACCAGAA
AGTAAACGTTACCAAATTGAGGGAATTAACTAAAATTGGTAATTTAGCTACTCCCTCCATGCTGGTTTTAGGAGATGTTG
TCAAATTGAGATGTATTTTATGGAAATCTAGTTGA

Upstream 100 bases:

>100_bases
CCTCATAAGTTTGATAAAAAGATCGAGGGTTATAGGGAATATCACGACTTGTTGAGAAAGCGTTTGGAAGAGGTGAATAG
GTATAGGTAAGGTAGTTTTA

Downstream 100 bases:

>100_bases
TAAATTGTTTAGAAACCCTATTATTCTTATTTTTAAATTTTGTGTTAAGTGCCAAATTTGCACATATCAATAGATATATA
ACTTACGGGGAATAGGAATA

Product: uroporphyrin III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGAGPGDPELITLKGIKYLQMADVIVYDKLVSRELISYAKPSCSVILLSSDDIEIDLLKKYALENKLVIRLKNGDPYVFG
RGGKICQELIKDGIECEVVPGVSSVNSVPAYAGIPLTFNGISDMITVISAVTQGGRLFDFKKIPDDGTLVVLMSGRRLEE
VSKELMTKRDPSEEVAVIQRGTYFDQKVNVTKLRELTKIGNLATPSMLVLGDVVKLRCILWKSS

Sequences:

>Translated_224_residues
MGAGPGDPELITLKGIKYLQMADVIVYDKLVSRELISYAKPSCSVILLSSDDIEIDLLKKYALENKLVIRLKNGDPYVFG
RGGKICQELIKDGIECEVVPGVSSVNSVPAYAGIPLTFNGISDMITVISAVTQGGRLFDFKKIPDDGTLVVLMSGRRLEE
VSKELMTKRDPSEEVAVIQRGTYFDQKVNVTKLRELTKIGNLATPSMLVLGDVVKLRCILWKSS
>Mature_223_residues
GAGPGDPELITLKGIKYLQMADVIVYDKLVSRELISYAKPSCSVILLSSDDIEIDLLKKYALENKLVIRLKNGDPYVFGR
GGKICQELIKDGIECEVVPGVSSVNSVPAYAGIPLTFNGISDMITVISAVTQGGRLFDFKKIPDDGTLVVLMSGRRLEEV
SKELMTKRDPSEEVAVIQRGTYFDQKVNVTKLRELTKIGNLATPSMLVLGDVVKLRCILWKSS

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=239, Percent_Identity=34.3096234309623, Blast_Score=130, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322922, Length=227, Percent_Identity=29.9559471365639, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 24568; Mature: 24437

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAGPGDPELITLKGIKYLQMADVIVYDKLVSRELISYAKPSCSVILLSSDDIEIDLLKK
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
YALENKLVIRLKNGDPYVFGRGGKICQELIKDGIECEVVPGVSSVNSVPAYAGIPLTFNG
HHCCCEEEEEEECCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEECC
ISDMITVISAVTQGGRLFDFKKIPDDGTLVVLMSGRRLEEVSKELMTKRDPSEEVAVIQR
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHEEEEEC
GTYFDQKVNVTKLRELTKIGNLATPSMLVLGDVVKLRCILWKSS
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEEECC
>Mature Secondary Structure 
GAGPGDPELITLKGIKYLQMADVIVYDKLVSRELISYAKPSCSVILLSSDDIEIDLLKK
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
YALENKLVIRLKNGDPYVFGRGGKICQELIKDGIECEVVPGVSSVNSVPAYAGIPLTFNG
HHCCCEEEEEEECCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEECC
ISDMITVISAVTQGGRLFDFKKIPDDGTLVVLMSGRRLEEVSKELMTKRDPSEEVAVIQR
HHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHCCCCCHHEEEEEC
GTYFDQKVNVTKLRELTKIGNLATPSMLVLGDVVKLRCILWKSS
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA