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The map label for this gene is 227828654

Identifier: 227828654

GI number: 227828654

Start: 2238079

End: 2239611

Strand: Direct

Name: 227828654

Synonym: M1425_2411

Alternate gene names: NA

Gene position: 2238079-2239611 (Clockwise)

Preceding gene: 227828653

Following gene: 227828655

Centisome position: 85.79

GC content: 39.14

Gene sequence:

>1533_bases
ATGACAAGTTGGGAAATAATTCACAAAGCCTCTGAGTTCATTGCAGAGGAAATGGGCGTTATGCTAAAGAGATCAGCCAT
GTCACCAAACATTAGGGAGAGGACGGATCATAGCTGTGCAATAACGGATGTCGAGGGTAATATTATAGCCCAAGCTGAAC
ACATACCAGTTCACCTAGGCTCTTTTAGCATTGGAGTTAAAAACACGTTAAACTTGATAGGTGAAATAGAAGAGAGTGAT
ATGGTTATTTTAAATGATCCTTATATATCTGGTACTCATCTGAATGACGTAATGGTTTTAGCCCCAGTTTACTATAATGG
TAAGCTAATAGGATATGTTGTAAATAAGGCACATCACGTTGATGTAGGTGGACCAATGCCAGGTAGTTTAAATCCATATG
CAACGACAATTTACGAGGAGGGTTTTGTAATACCCCCAGTGAGGTTGATGAGGAAAGGGCAGATAAATAATGAGATTATT
AATATAATTAGGCATAACTTCAAGGTACCCGAGGTATCAATAGGCGATCTGAACGCTCAAATTTCAGGGAACCTCAATGG
TATAGCTAGGATTAGGCAAATGTTTGATAAGTACGGTTACGAGAACGTTCTTAATGCGTGGAAGATCTCAATGGAGTATG
GGAAGAAATTGGCGTTATCTGAAATTTCAAAATGGGGTAAGGGTGTTGGTGAGGATGAAGATTATTTAGAAATAGGTGAT
GACCTAAAGAAAATAAGGCTAAAGGTAAAGATTGATGAGAATGGTGTTTACGCTAATTTTGCTGGAACTGATACCCAGAT
TGAGGGACCTTTAAATGCAGTATATGGAGTTACGTTTTCGGCTGTTAGTTTTGTCATAAGGTCATTAATAGGAAAGGATA
TTCCTACTAATGAGGGCTTTTACAGTCTAATTAATGTTGATGCGCCTAAGGGTACTATAGTTAATCCCAATAAGCCAGCA
GCAGTTGGTGGTGGAAACGTAGAGACTTCACAGAGAATTGCCGATGTTACCTTTAAGGCTTTATCTCACCTTATTGACGT
TCCCGCTGCTGGTTCTGGTACTATGATGAATGTTATGATGGGTGGAATTTATAGGGGTAAATATTGGGCTTACTATGAGA
CAATAGGTGGTGGTACTGGAGCGAGGCCAAATAAGGATGGTGTTTCAGCTGTTCAAGTTAACATGACTAATACTTTGAAT
ACTCCCATTGAAATTGCTGAAAGGTATTATCCAATAATGTTTACTACGTATCGAATAAGGGATGGTAGTGGTGGTGATGG
CAAGTATAAGGGTGGTGATGGTATAATTAGGGCATTTAAGATTCTGGAAAAGACTAGACTATCAATAATCGCGGAGAGAT
TTAAGATTGCCCCTTGGGGTTTAAAGGGTGGTAGGAACGGTAAGCCTGCAAGGGTTACAATAATAAGGGTTGATTGTAGA
AAGGAGGAAATGCCTAGTAAGTTTTCAACAATTTTGAATAAAGGTGATGAGGTGATAATTGAAACTCCAGGTGGCGGAGG
ATATTATGGTTAA

Upstream 100 bases:

>100_bases
GAATAAGGGGGCCAGCAATAATTGAGGAATACAGCTCGACTACAGTTATAAAGGATGGTTGGAATGCAATTATAGATGAT
TCAATAATATTGGTGAGAGA

Downstream 100 bases:

>100_bases
GGGTTAAATATTTTTGTAGTTTTTAGTTTCTAGTGGATAAACAATTTACATTTATTAAGAATGAATCTTTGATGAATCTG
ACTTTCATACCGCCATAAAC

Product: 5-oxoprolinase (ATP-hydrolyzing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTSWEIIHKASEFIAEEMGVMLKRSAMSPNIRERTDHSCAITDVEGNIIAQAEHIPVHLGSFSIGVKNTLNLIGEIEESD
MVILNDPYISGTHLNDVMVLAPVYYNGKLIGYVVNKAHHVDVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGQINNEII
NIIRHNFKVPEVSIGDLNAQISGNLNGIARIRQMFDKYGYENVLNAWKISMEYGKKLALSEISKWGKGVGEDEDYLEIGD
DLKKIRLKVKIDENGVYANFAGTDTQIEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGFYSLINVDAPKGTIVNPNKPA
AVGGGNVETSQRIADVTFKALSHLIDVPAAGSGTMMNVMMGGIYRGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLN
TPIEIAERYYPIMFTTYRIRDGSGGDGKYKGGDGIIRAFKILEKTRLSIIAERFKIAPWGLKGGRNGKPARVTIIRVDCR
KEEMPSKFSTILNKGDEVIIETPGGGGYYG

Sequences:

>Translated_510_residues
MTSWEIIHKASEFIAEEMGVMLKRSAMSPNIRERTDHSCAITDVEGNIIAQAEHIPVHLGSFSIGVKNTLNLIGEIEESD
MVILNDPYISGTHLNDVMVLAPVYYNGKLIGYVVNKAHHVDVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGQINNEII
NIIRHNFKVPEVSIGDLNAQISGNLNGIARIRQMFDKYGYENVLNAWKISMEYGKKLALSEISKWGKGVGEDEDYLEIGD
DLKKIRLKVKIDENGVYANFAGTDTQIEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGFYSLINVDAPKGTIVNPNKPA
AVGGGNVETSQRIADVTFKALSHLIDVPAAGSGTMMNVMMGGIYRGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLN
TPIEIAERYYPIMFTTYRIRDGSGGDGKYKGGDGIIRAFKILEKTRLSIIAERFKIAPWGLKGGRNGKPARVTIIRVDCR
KEEMPSKFSTILNKGDEVIIETPGGGGYYG
>Mature_509_residues
TSWEIIHKASEFIAEEMGVMLKRSAMSPNIRERTDHSCAITDVEGNIIAQAEHIPVHLGSFSIGVKNTLNLIGEIEESDM
VILNDPYISGTHLNDVMVLAPVYYNGKLIGYVVNKAHHVDVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGQINNEIIN
IIRHNFKVPEVSIGDLNAQISGNLNGIARIRQMFDKYGYENVLNAWKISMEYGKKLALSEISKWGKGVGEDEDYLEIGDD
LKKIRLKVKIDENGVYANFAGTDTQIEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGFYSLINVDAPKGTIVNPNKPAA
VGGGNVETSQRIADVTFKALSHLIDVPAAGSGTMMNVMMGGIYRGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLNT
PIEIAERYYPIMFTTYRIRDGSGGDGKYKGGDGIIRAFKILEKTRLSIIAERFKIAPWGLKGGRNGKPARVTIIRVDCRK
EEMPSKFSTILNKGDEVIIETPGGGGYYG

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=516, Percent_Identity=35.8527131782946, Blast_Score=297, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI133901900, Length=519, Percent_Identity=38.3429672447013, Blast_Score=317, Evalue=9e-87,
Organism=Caenorhabditis elegans, GI133901902, Length=503, Percent_Identity=37.375745526839, Blast_Score=295, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6322634, Length=524, Percent_Identity=35.8778625954198, Blast_Score=301, Evalue=1e-82,
Organism=Drosophila melanogaster, GI45550492, Length=519, Percent_Identity=35.2601156069364, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 55856; Mature: 55725

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSWEIIHKASEFIAEEMGVMLKRSAMSPNIRERTDHSCAITDVEGNIIAQAEHIPVHLG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCEEEEECCCEEEEC
SFSIGVKNTLNLIGEIEESDMVILNDPYISGTHLNDVMVLAPVYYNGKLIGYVVNKAHHV
CEECCHHHHHHHHCCCCCCCEEEEECCEECCCCCCCEEEEEEEEECCEEEEEEECCCEEE
DVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGQINNEIINIIRHNFKVPEVSIGDLNAQ
ECCCCCCCCCCCCEEEEECCCEECCCHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCEE
ISGNLNGIARIRQMFDKYGYENVLNAWKISMEYGKKLALSEISKWGKGVGEDEDYLEIGD
ECCCCHHHHHHHHHHHHCCHHHHHHHHEEEHHHCCCHHHHHHHHHCCCCCCCCHHHHHCC
DLKKIRLKVKIDENGVYANFAGTDTQIEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGF
CEEEEEEEEEECCCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YSLINVDAPKGTIVNPNKPAAVGGGNVETSQRIADVTFKALSHLIDVPAAGSGTMMNVMM
EEEEEECCCCCCEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
GGIYRGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLNTPIEIAERYYPIMFTTYRIR
CCEECCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEEEEE
DGSGGDGKYKGGDGIIRAFKILEKTRLSIIAERFKIAPWGLKGGRNGKPARVTIIRVDCR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEEECC
KEEMPSKFSTILNKGDEVIIETPGGGGYYG
CCCCCHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
TSWEIIHKASEFIAEEMGVMLKRSAMSPNIRERTDHSCAITDVEGNIIAQAEHIPVHLG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEECCCCEEEEECCCEEEEC
SFSIGVKNTLNLIGEIEESDMVILNDPYISGTHLNDVMVLAPVYYNGKLIGYVVNKAHHV
CEECCHHHHHHHHCCCCCCCEEEEECCEECCCCCCCEEEEEEEEECCEEEEEEECCCEEE
DVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGQINNEIINIIRHNFKVPEVSIGDLNAQ
ECCCCCCCCCCCCEEEEECCCEECCCHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCEE
ISGNLNGIARIRQMFDKYGYENVLNAWKISMEYGKKLALSEISKWGKGVGEDEDYLEIGD
ECCCCHHHHHHHHHHHHCCHHHHHHHHEEEHHHCCCHHHHHHHHHCCCCCCCCHHHHHCC
DLKKIRLKVKIDENGVYANFAGTDTQIEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGF
CEEEEEEEEEECCCCEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YSLINVDAPKGTIVNPNKPAAVGGGNVETSQRIADVTFKALSHLIDVPAAGSGTMMNVMM
EEEEEECCCCCCEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
GGIYRGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLNTPIEIAERYYPIMFTTYRIR
CCEECCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEEEEEE
DGSGGDGKYKGGDGIIRAFKILEKTRLSIIAERFKIAPWGLKGGRNGKPARVTIIRVDCR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCEEEEEEEEECC
KEEMPSKFSTILNKGDEVIIETPGGGGYYG
CCCCCHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]