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The map label for this gene is 227828653

Identifier: 227828653

GI number: 227828653

Start: 2236139

End: 2238082

Strand: Direct

Name: 227828653

Synonym: M1425_2410

Alternate gene names: NA

Gene position: 2236139-2238082 (Clockwise)

Preceding gene: 227828652

Following gene: 227828654

Centisome position: 85.71

GC content: 39.2

Gene sequence:

>1944_bases
ATGGAGTGTAAAGTGGCAATAGATATTGGTGGGACGTTTACTGATTTCATAGTCCTATCCAGTGAGGGGGAAATTAAGAC
AATAAAAGTCCTAACTAACCCTAAAGACCCTGGACAAGTCATTAAAGATGTAATAAGCACTTTAAACTGCAACGTAAATG
AGATAGCTCACGCCACGACACTTGCAACAAATACGTTATTAGGGCAGGAAAACCTGACCATCTCAAAGACTGCCTTACTC
GTTACAAAAGGATTCAAGGATATTATCGAAATAGGTAGACAGAATAGGCCTAGGCTTTATGATTTACATTTTGAAAAGCC
AAGACAATTGGTCCTCAGAAGTTTAAGATTTGAGATTGACGAGAGGATCAACGCTGAAGGGAATATAATAAAGGAGGTGA
ATCCAGACGAAATAGAGAGTATTTCTAAAAAACTTTTAGACGAAAATGTAATATCGATTGCAGTAAGTTATCTGCACTCT
TATCTTAATCCAAAAAATGAGCTAATTACTGAAAATGTGTTAAAGAAGTACTTCAACTACATCTCAATCTCCTCAAAAAT
AGCACCAGAACCTAGAGAATACGAGAGGACTTCTACTACAGTAGTTAATGCAGTGCTAATGCCATTAGTATCTGCATATC
TTGAGAAATTACAAGCCTCTTTACCAACTGAAAACTTCTATATTATGTCAAGTTCTGGAGGATTAGTAGATATTAAAGAA
GCGTCTGAGAAGCCAGTACAGCTAATTGAATCCGGACCAGCAGCTGGTTTAATAGCTTCGGCTAGCTTCTTGCCTAATGA
GAGTTTTATAAGCTTTGACATGGGGGGTACTACCGCTAAGGCAGGTGTTATAATTAATGGTAATTTTGAAATGACCACTG
AGTATGAAGTTGGAGGGGAAGTACACTATGGAAGGATAGTTAAGGGTACCGGGTATCCAGTAAGGTTCCCATTTGTTGAT
TTGGCCGAAGTATCTGCTGGTGGGGGTACGATAATATGGAGGGACGAGGCTAATGCTTTAAAAGTTGGACCTATAAGTGC
TGGTGCTGATCCTGGACCAATTTCCTACGGAAAGGGAGGAAATAGGCCTACAATAGCTGATGCCAATCTGATTTTAGGAA
GATTGGGGACAGAGCTCATTGGTGGTAATCTAAAGTTAGATAAAGAGAGTGCAATAAAGGGGATGAGTAGATTAGGCGAT
CCATATGAGGTAAGTAAGAATGCTTTAGAATTGATAAACTTGGAGATGGCTAGAGCAATAAGGTTAGTTACAGTGGAGAG
GGGATTAGACCCATCAAACTTCTCCTTAATAGCCTTTGGAGGTGCTGGTCCGCAACATGCATTATATTTGGCTGAGGAGG
TGGGGATAGGTAAGGTTATAATATCACCTCATCCAGGATTATTCAGTGCTTTAGGATTATTACTAGCTGATTGGCGTTTT
GAAGTTAGGAAATCATATCCTAAAGACTTAGAGAGTGAATTTAAGCGATTGGAAAAGGAGCTATATGATAAGTTGAAGGG
GAACGTCGACTACTTCTTGAGACTTGCAGATGCGAGATATGAGGGACAAGGATGGGAGTTAACAATTCCAGTAAATAACG
TTCATGAGATTAGGAAAGTGTTTGAGGAGAAGCACCTAGCGACTTATGGTTTCGTGATGAATGATAGGGAGATAGAGATT
GTTACAATAAGGGTTTTCGCTATCAAGAAGAGACCATTACCTAAAATAAAGGTAACATTTGGAAATTGTGATAAGCCAAG
GGAGGTTAGGAAAGTCATGATTGATGACGATTGGGTAAATGTCGACGTCTATGTTAGGGAGGAGTTATCTAAAGGCTTTA
GAATAAGGGGGCCAGCAATAATTGAGGAATACAGCTCGACTACAGTTATAAAGGATGGTTGGAATGCAATTATAGATGAT
TCAATAATATTGGTGAGAGAATGA

Upstream 100 bases:

>100_bases
CACCTTGTTCACCTAATTGCAAACTTATAAGAGCTAGATTAATAAACGGTGAGGTATTGTTTGATAAGCCATTTATACCC
AAAACAAAGGGTTGATAAAT

Downstream 100 bases:

>100_bases
CAAGTTGGGAAATAATTCACAAAGCCTCTGAGTTCATTGCAGAGGAAATGGGCGTTATGCTAAAGAGATCAGCCATGTCA
CCAAACATTAGGGAGAGGAC

Product: 5-oxoprolinase (ATP-hydrolyzing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MECKVAIDIGGTFTDFIVLSSEGEIKTIKVLTNPKDPGQVIKDVISTLNCNVNEIAHATTLATNTLLGQENLTISKTALL
VTKGFKDIIEIGRQNRPRLYDLHFEKPRQLVLRSLRFEIDERINAEGNIIKEVNPDEIESISKKLLDENVISIAVSYLHS
YLNPKNELITENVLKKYFNYISISSKIAPEPREYERTSTTVVNAVLMPLVSAYLEKLQASLPTENFYIMSSSGGLVDIKE
ASEKPVQLIESGPAAGLIASASFLPNESFISFDMGGTTAKAGVIINGNFEMTTEYEVGGEVHYGRIVKGTGYPVRFPFVD
LAEVSAGGGTIIWRDEANALKVGPISAGADPGPISYGKGGNRPTIADANLILGRLGTELIGGNLKLDKESAIKGMSRLGD
PYEVSKNALELINLEMARAIRLVTVERGLDPSNFSLIAFGGAGPQHALYLAEEVGIGKVIISPHPGLFSALGLLLADWRF
EVRKSYPKDLESEFKRLEKELYDKLKGNVDYFLRLADARYEGQGWELTIPVNNVHEIRKVFEEKHLATYGFVMNDREIEI
VTIRVFAIKKRPLPKIKVTFGNCDKPREVRKVMIDDDWVNVDVYVREELSKGFRIRGPAIIEEYSSTTVIKDGWNAIIDD
SIILVRE

Sequences:

>Translated_647_residues
MECKVAIDIGGTFTDFIVLSSEGEIKTIKVLTNPKDPGQVIKDVISTLNCNVNEIAHATTLATNTLLGQENLTISKTALL
VTKGFKDIIEIGRQNRPRLYDLHFEKPRQLVLRSLRFEIDERINAEGNIIKEVNPDEIESISKKLLDENVISIAVSYLHS
YLNPKNELITENVLKKYFNYISISSKIAPEPREYERTSTTVVNAVLMPLVSAYLEKLQASLPTENFYIMSSSGGLVDIKE
ASEKPVQLIESGPAAGLIASASFLPNESFISFDMGGTTAKAGVIINGNFEMTTEYEVGGEVHYGRIVKGTGYPVRFPFVD
LAEVSAGGGTIIWRDEANALKVGPISAGADPGPISYGKGGNRPTIADANLILGRLGTELIGGNLKLDKESAIKGMSRLGD
PYEVSKNALELINLEMARAIRLVTVERGLDPSNFSLIAFGGAGPQHALYLAEEVGIGKVIISPHPGLFSALGLLLADWRF
EVRKSYPKDLESEFKRLEKELYDKLKGNVDYFLRLADARYEGQGWELTIPVNNVHEIRKVFEEKHLATYGFVMNDREIEI
VTIRVFAIKKRPLPKIKVTFGNCDKPREVRKVMIDDDWVNVDVYVREELSKGFRIRGPAIIEEYSSTTVIKDGWNAIIDD
SIILVRE
>Mature_647_residues
MECKVAIDIGGTFTDFIVLSSEGEIKTIKVLTNPKDPGQVIKDVISTLNCNVNEIAHATTLATNTLLGQENLTISKTALL
VTKGFKDIIEIGRQNRPRLYDLHFEKPRQLVLRSLRFEIDERINAEGNIIKEVNPDEIESISKKLLDENVISIAVSYLHS
YLNPKNELITENVLKKYFNYISISSKIAPEPREYERTSTTVVNAVLMPLVSAYLEKLQASLPTENFYIMSSSGGLVDIKE
ASEKPVQLIESGPAAGLIASASFLPNESFISFDMGGTTAKAGVIINGNFEMTTEYEVGGEVHYGRIVKGTGYPVRFPFVD
LAEVSAGGGTIIWRDEANALKVGPISAGADPGPISYGKGGNRPTIADANLILGRLGTELIGGNLKLDKESAIKGMSRLGD
PYEVSKNALELINLEMARAIRLVTVERGLDPSNFSLIAFGGAGPQHALYLAEEVGIGKVIISPHPGLFSALGLLLADWRF
EVRKSYPKDLESEFKRLEKELYDKLKGNVDYFLRLADARYEGQGWELTIPVNNVHEIRKVFEEKHLATYGFVMNDREIEI
VTIRVFAIKKRPLPKIKVTFGNCDKPREVRKVMIDDDWVNVDVYVREELSKGFRIRGPAIIEEYSSTTVIKDGWNAIIDD
SIILVRE

Specific function: Unknown

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=727, Percent_Identity=29.1609353507565, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI133901900, Length=699, Percent_Identity=28.6123032904149, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322634, Length=747, Percent_Identity=30.6559571619813, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI45550492, Length=724, Percent_Identity=28.4530386740331, Blast_Score=228, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 71698; Mature: 71698

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECKVAIDIGGTFTDFIVLSSEGEIKTIKVLTNPKDPGQVIKDVISTLNCNVNEIAHATT
CCEEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
LATNTLLGQENLTISKTALLVTKGFKDIIEIGRQNRPRLYDLHFEKPRQLVLRSLRFEID
HHHHHEECCCCCEEEHEEEEEHHCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
ERINAEGNIIKEVNPDEIESISKKLLDENVISIAVSYLHSYLNPKNELITENVLKKYFNY
HHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ISISSKIAPEPREYERTSTTVVNAVLMPLVSAYLEKLQASLPTENFYIMSSSGGLVDIKE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECC
ASEKPVQLIESGPAAGLIASASFLPNESFISFDMGGTTAKAGVIINGNFEMTTEYEVGGE
CCCCCHHHHHCCCCCCEEEEHHCCCCCCEEEEECCCCCEECCEEEECCEEEEEEEECCCE
VHYGRIVKGTGYPVRFPFVDLAEVSAGGGTIIWRDEANALKVGPISAGADPGPISYGKGG
EEECEEEECCCCEEECCCCCHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCC
NRPTIADANLILGRLGTELIGGNLKLDKESAIKGMSRLGDPYEVSKNALELINLEMARAI
CCCCEECHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHE
RLVTVERGLDPSNFSLIAFGGAGPQHALYLAEEVGIGKVIISPHPGLFSALGLLLADWRF
EEEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHH
EVRKSYPKDLESEFKRLEKELYDKLKGNVDYFLRLADARYEGQGWELTIPVNNVHEIRKV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEECCCCEEEEEECCCHHHHHHH
FEEKHLATYGFVMNDREIEIVTIRVFAIKKRPLPKIKVTFGNCDKPREVRKVMIDDDWVN
HHHHCCEEEEEEECCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHEEEECCCEEE
VDVYVREELSKGFRIRGPAIIEEYSSTTVIKDGWNAIIDDSIILVRE
EEEEEEHHHCCCCEECCCHHHHHCCCCEEEECCCCEEECCCEEEEEC
>Mature Secondary Structure
MECKVAIDIGGTFTDFIVLSSEGEIKTIKVLTNPKDPGQVIKDVISTLNCNVNEIAHATT
CCEEEEEEECCCEEEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHH
LATNTLLGQENLTISKTALLVTKGFKDIIEIGRQNRPRLYDLHFEKPRQLVLRSLRFEID
HHHHHEECCCCCEEEHEEEEEHHCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHH
ERINAEGNIIKEVNPDEIESISKKLLDENVISIAVSYLHSYLNPKNELITENVLKKYFNY
HHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ISISSKIAPEPREYERTSTTVVNAVLMPLVSAYLEKLQASLPTENFYIMSSSGGLVDIKE
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECC
ASEKPVQLIESGPAAGLIASASFLPNESFISFDMGGTTAKAGVIINGNFEMTTEYEVGGE
CCCCCHHHHHCCCCCCEEEEHHCCCCCCEEEEECCCCCEECCEEEECCEEEEEEEECCCE
VHYGRIVKGTGYPVRFPFVDLAEVSAGGGTIIWRDEANALKVGPISAGADPGPISYGKGG
EEECEEEECCCCEEECCCCCHHHCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCC
NRPTIADANLILGRLGTELIGGNLKLDKESAIKGMSRLGDPYEVSKNALELINLEMARAI
CCCCEECHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHE
RLVTVERGLDPSNFSLIAFGGAGPQHALYLAEEVGIGKVIISPHPGLFSALGLLLADWRF
EEEEEECCCCCCCEEEEEECCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHH
EVRKSYPKDLESEFKRLEKELYDKLKGNVDYFLRLADARYEGQGWELTIPVNNVHEIRKV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEECCCCEEEEEECCCHHHHHHH
FEEKHLATYGFVMNDREIEIVTIRVFAIKKRPLPKIKVTFGNCDKPREVRKVMIDDDWVN
HHHHCCEEEEEEECCCEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHEEEECCCEEE
VDVYVREELSKGFRIRGPAIIEEYSSTTVIKDGWNAIIDDSIILVRE
EEEEEEHHHCCCCEECCCHHHHHCCCCEEEECCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]