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The map label for this gene is uidA [H]

Identifier: 227828568

GI number: 227828568

Start: 2133159

End: 2134868

Strand: Direct

Name: uidA [H]

Synonym: M1425_2319

Alternate gene names: 227828568

Gene position: 2133159-2134868 (Clockwise)

Preceding gene: 227828566

Following gene: 227828569

Centisome position: 81.77

GC content: 35.85

Gene sequence:

>1710_bases
ATGAGATCGTTTTATAGGCCAAAAATAGATCTTCAAGGCTTTTGGAAATTCAAGATAGATAGTGAGAATGTTGGGGAAAA
GAACGAGTGGGGTAAAGGTTTAGAATCTGAAGATATTATTTACGTGCCAGCTTCTTGGAATGAGCAAAATCCACAATGGG
ATCAGTTTTCTAGTATAGCGTGGTATCAGAAGGATCTGTTTGTTAGTAGTTATGAGAGTAAGAAGGCTTGGATAGTATTT
GAGGGGGCAGGATACATCACAAAACTTTGGATTAATGGAGAATATGTAGGAACTCATGAGGGATCATTTACACAATTCAA
ATTTCCAATTAAACTTAATACTAATGAATTTAATAAAATAGTAGTAAAGATAGATAACACGCCATCACCCTACAACTTAC
CCCCAGCTAAAGATCTAAACATTGCAGCGTTTGACTTCTTCAATTATGGAGGGATCCAGAGACCAGTTTACATAGAATTT
GTAGATGAATGTCACGTAGAAGATATAACAGTATATACTAAATCCAATGGACATCTAAAGGTAGAGATTTTGCCCGAATG
TAATCAAAAGTACAGCCTAAGATTTAAATTAGTTGATAAAGAGGGAAAAGTCCTGTTAGATGAGGAGAGTAGTAACGAAA
TATATGAAAAAGATGTAGGTAATGTTACGCCGTGGTCTTCAGATAATCCTTACTTATACACTCTCATAGTGGAAATATAC
GTTGATGAAAATCTGAAGGACTCGATTTATGAACGTATAGGCTTTAGAGATGTAGAGGTGAAGAATGGCAGAATATACTT
GAACCGTAAGCCCATATTCTTAAAGGGCTTTGGAAGACATGAGGATTTTCCAATACTAGGTAAATTTATGTACGGGGCAG
TATTAGTTAGGGATTTTTACCTTATGAGAAAAATTGGAGCTAACTCGTTTAGAACTTCCCACTACCCTTATTCAAATGAA
CATTTAGATTTAGCCGATGAAATGGGATTTTTGGTCATATTAGAACCACCTTTATGTTATTCCAATATTAGTAGGGTGAT
GAGCCAAGAGGAGATTGCTAAGATGTTCAGCGATATAAAGTACTTCGAAAAGGTAAAGCATACGATAAAGGAAATGATTA
GACAACATAAGAATAGACCTTCAGTAATAATGTATAGCGTTATGAATGAACCCCCTAGCGATATGCGTGAAGTTGCAGAA
TTTATAAAGAAGGAAGTTGAATTCTTGAAGACTTTGGATTCTTCTAGGCCAGTAACTTTCGCCTCTCATAGATCAGTACG
GGACCTAGCGTTAGAATACGTTGATGTAATCTCCTTAAACTATTATCATGGATGGTATACTGAATGGGGGGATATTGATA
GCGGTGTAAAGATAGTCGCAATGGAATTAGAGGAAATTCATAAGAAGTTTCCAGAGAAGCCAATAATCATTACCGAATTT
GGTGCTGATGCCATATATGGCCTTCATAGTGATCCTCCCCAAATGTGGTCAGAGGAATACCAAAGTGAAATGATAAGAAA
GTATATAGAAACCTTAAGGATGAACAGTTATATAGTAGGCTTTCACATTTGGAACTTTGCTGACTTTAGAACTCCTCAAA
ATCCAGGGAGAACAATACTTAATAGAAAAGGAATATTTACTAGAGATAGGCAGCCTAAATCGGCAGCTAAAGTGGTTGAG
GAATTATTTAAAAATAAATTGCCTTCTTAA

Upstream 100 bases:

>100_bases
AGTATATGAGATTGCCTATTTAATTCTTTAGCGTTACTATTTAGAAGTTTCTAATATTTTAATAAAGCTTTAATTACACT
CTCTTGTTTGTAGATACTAT

Downstream 100 bases:

>100_bases
GGTGATGTGAGCGTATATGTTCTACTTTCGAAATATTGATAGCGTCTATAAGTATAAACATAGTATTGCCGCTAACATAT
CATTTTTCTATTCTATTATG

Product: beta-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRSFYRPKIDLQGFWKFKIDSENVGEKNEWGKGLESEDIIYVPASWNEQNPQWDQFSSIAWYQKDLFVSSYESKKAWIVF
EGAGYITKLWINGEYVGTHEGSFTQFKFPIKLNTNEFNKIVVKIDNTPSPYNLPPAKDLNIAAFDFFNYGGIQRPVYIEF
VDECHVEDITVYTKSNGHLKVEILPECNQKYSLRFKLVDKEGKVLLDEESSNEIYEKDVGNVTPWSSDNPYLYTLIVEIY
VDENLKDSIYERIGFRDVEVKNGRIYLNRKPIFLKGFGRHEDFPILGKFMYGAVLVRDFYLMRKIGANSFRTSHYPYSNE
HLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFSDIKYFEKVKHTIKEMIRQHKNRPSVIMYSVMNEPPSDMREVAE
FIKKEVEFLKTLDSSRPVTFASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKIVAMELEEIHKKFPEKPIIITEF
GADAIYGLHSDPPQMWSEEYQSEMIRKYIETLRMNSYIVGFHIWNFADFRTPQNPGRTILNRKGIFTRDRQPKSAAKVVE
ELFKNKLPS

Sequences:

>Translated_569_residues
MRSFYRPKIDLQGFWKFKIDSENVGEKNEWGKGLESEDIIYVPASWNEQNPQWDQFSSIAWYQKDLFVSSYESKKAWIVF
EGAGYITKLWINGEYVGTHEGSFTQFKFPIKLNTNEFNKIVVKIDNTPSPYNLPPAKDLNIAAFDFFNYGGIQRPVYIEF
VDECHVEDITVYTKSNGHLKVEILPECNQKYSLRFKLVDKEGKVLLDEESSNEIYEKDVGNVTPWSSDNPYLYTLIVEIY
VDENLKDSIYERIGFRDVEVKNGRIYLNRKPIFLKGFGRHEDFPILGKFMYGAVLVRDFYLMRKIGANSFRTSHYPYSNE
HLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFSDIKYFEKVKHTIKEMIRQHKNRPSVIMYSVMNEPPSDMREVAE
FIKKEVEFLKTLDSSRPVTFASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKIVAMELEEIHKKFPEKPIIITEF
GADAIYGLHSDPPQMWSEEYQSEMIRKYIETLRMNSYIVGFHIWNFADFRTPQNPGRTILNRKGIFTRDRQPKSAAKVVE
ELFKNKLPS
>Mature_569_residues
MRSFYRPKIDLQGFWKFKIDSENVGEKNEWGKGLESEDIIYVPASWNEQNPQWDQFSSIAWYQKDLFVSSYESKKAWIVF
EGAGYITKLWINGEYVGTHEGSFTQFKFPIKLNTNEFNKIVVKIDNTPSPYNLPPAKDLNIAAFDFFNYGGIQRPVYIEF
VDECHVEDITVYTKSNGHLKVEILPECNQKYSLRFKLVDKEGKVLLDEESSNEIYEKDVGNVTPWSSDNPYLYTLIVEIY
VDENLKDSIYERIGFRDVEVKNGRIYLNRKPIFLKGFGRHEDFPILGKFMYGAVLVRDFYLMRKIGANSFRTSHYPYSNE
HLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFSDIKYFEKVKHTIKEMIRQHKNRPSVIMYSVMNEPPSDMREVAE
FIKKEVEFLKTLDSSRPVTFASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKIVAMELEEIHKKFPEKPIIITEF
GADAIYGLHSDPPQMWSEEYQSEMIRKYIETLRMNSYIVGFHIWNFADFRTPQNPGRTILNRKGIFTRDRQPKSAAKVVE
ELFKNKLPS

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=601, Percent_Identity=33.2778702163062, Blast_Score=298, Evalue=1e-80,
Organism=Escherichia coli, GI1787903, Length=579, Percent_Identity=34.3696027633851, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI48994920, Length=398, Percent_Identity=26.1306532663317, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1786539, Length=366, Percent_Identity=25.4098360655738, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17510825, Length=599, Percent_Identity=30.7178631051753, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI19922582, Length=599, Percent_Identity=32.220367278798, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI62471735, Length=598, Percent_Identity=32.1070234113712, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24655438, Length=598, Percent_Identity=32.1070234113712, Blast_Score=288, Evalue=9e-78,
Organism=Drosophila melanogaster, GI24651745, Length=565, Percent_Identity=31.858407079646, Blast_Score=284, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 66737; Mature: 66737

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFYRPKIDLQGFWKFKIDSENVGEKNEWGKGLESEDIIYVPASWNEQNPQWDQFSSIA
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCHHHHCCHH
WYQKDLFVSSYESKKAWIVFEGAGYITKLWINGEYVGTHEGSFTQFKFPIKLNTNEFNKI
HHHHHHEEECCCCCEEEEEEECCCEEEEEEECCEEEECCCCCEEEEEEEEEECCCCCEEE
VVKIDNTPSPYNLPPAKDLNIAAFDFFNYGGIQRPVYIEFVDECHVEDITVYTKSNGHLK
EEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCEEE
VEILPECNQKYSLRFKLVDKEGKVLLDEESSNEIYEKDVGNVTPWSSDNPYLYTLIVEIY
EEECCCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEEEEEE
VDENLKDSIYERIGFRDVEVKNGRIYLNRKPIFLKGFGRHEDFPILGKFMYGAVLVRDFY
ECCCHHHHHHHHCCCEEEEEECCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LMRKIGANSFRTSHYPYSNEHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFSDIK
HHHHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
YFEKVKHTIKEMIRQHKNRPSVIMYSVMNEPPSDMREVAEFIKKEVEFLKTLDSSRPVTF
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKIVAMELEEIHKKFPEKPIIITEF
ECCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEEC
GADAIYGLHSDPPQMWSEEYQSEMIRKYIETLRMNSYIVGFHIWNFADFRTPQNPGRTIL
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCCCCCHHH
NRKGIFTRDRQPKSAAKVVEELFKNKLPS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRSFYRPKIDLQGFWKFKIDSENVGEKNEWGKGLESEDIIYVPASWNEQNPQWDQFSSIA
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCHHHHCCHH
WYQKDLFVSSYESKKAWIVFEGAGYITKLWINGEYVGTHEGSFTQFKFPIKLNTNEFNKI
HHHHHHEEECCCCCEEEEEEECCCEEEEEEECCEEEECCCCCEEEEEEEEEECCCCCEEE
VVKIDNTPSPYNLPPAKDLNIAAFDFFNYGGIQRPVYIEFVDECHVEDITVYTKSNGHLK
EEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCEEEEEECCCCEEE
VEILPECNQKYSLRFKLVDKEGKVLLDEESSNEIYEKDVGNVTPWSSDNPYLYTLIVEIY
EEECCCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHCCCCCCCCCCCCCEEEEEEEEEE
VDENLKDSIYERIGFRDVEVKNGRIYLNRKPIFLKGFGRHEDFPILGKFMYGAVLVRDFY
ECCCHHHHHHHHCCCEEEEEECCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHH
LMRKIGANSFRTSHYPYSNEHLDLADEMGFLVILEPPLCYSNISRVMSQEEIAKMFSDIK
HHHHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
YFEKVKHTIKEMIRQHKNRPSVIMYSVMNEPPSDMREVAEFIKKEVEFLKTLDSSRPVTF
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
ASHRSVRDLALEYVDVISLNYYHGWYTEWGDIDSGVKIVAMELEEIHKKFPEKPIIITEF
ECCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCEEEEEC
GADAIYGLHSDPPQMWSEEYQSEMIRKYIETLRMNSYIVGFHIWNFADFRTPQNPGRTIL
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCHHCCCCCCCCCHHH
NRKGIFTRDRQPKSAAKVVEELFKNKLPS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]