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The map label for this gene is strD [H]

Identifier: 227828398

GI number: 227828398

Start: 1959973

End: 1960734

Strand: Direct

Name: strD [H]

Synonym: M1425_2141

Alternate gene names: 227828398

Gene position: 1959973-1960734 (Clockwise)

Preceding gene: 227828397

Following gene: 227828399

Centisome position: 75.13

GC content: 34.51

Gene sequence:

>762_bases
ATGCTTAAAAAAGCCGTAATCACATCTGCTGGAAAAGGTAGTAGGATGAAACATATAACGAGTGTATTACCCAAGGCATT
ACTTCCTTTATTCGTCACCGAAAACGGAGGTAAGGTAACTAGACCAGTTATAGATTTAATAATGGATTCATTGAACAAGG
TAGGTATTGAAAAATTTTGTATTGTAGTAGGAAGAAACGGAATGTTACTAATGCAATACTTATTCGATAGAACACCAACC
TTTGTATTCCAAGATATGCCTAAGGGATTTGGTGACGCTGTATTAAGAGCAGAGGACTTTTCATCCAATGAGCCATTTTT
CGTTCATGCAGACGATGGCGTTCTAACTAAAGGATACGAGTCGCTTAAATTGCTATTTGATGAAGTAAGTCCAGATGCTG
TACTTTTCGTGAGAAGAGTTGAAAATCCTAGTAGATACGGTGTGGTAACTATCCAAGATAAAGGGGTATATGATGGTCAT
AAATTATATAAGGTAATTGACGCCGAAGAAAAGCCATTACATCCAAAATCTAATTTAGCCATAGCAGCGGTTTATATTTT
TAAGCCTAGTATATTTAGTGCGTTGAAACAAATTAATGTTGAAGAAGGTAAAGAGATAGAATTGACTTATGGAATACATA
ACTTGTTGCTAGATGGTAAAGAAGTTTATGCGTTAGAAATGAATGAAGATGAAAAATGGTTGAATGTGGGAGATCCTAAA
AGTTATCTTGACGCATTAAACTATTCATTTAAATTTCTTTAA

Upstream 100 bases:

>100_bases
TTCTAGCAGCGGAGACGTTAAGTATGATAGAAGAGGAAAATGAAGAAGAAGAATAGGTTTGCAATGAGTTTATATAATGT
GAAAATTTTAATATCAATTG

Downstream 100 bases:

>100_bases
CAACAAAATATATTTGATGATACTTGTTGGAGTAGATGGCGGAGGAAGTAAAACAAGCGCAATAGCCTATACTTGTAGTG
GGTCTTTTTTAGGAAAAGGT

Product: nucleotidyl transferase

Products: NA

Alternate protein names: Sugar-nucleotidylation enzyme; dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLKKAVITSAGKGSRMKHITSVLPKALLPLFVTENGGKVTRPVIDLIMDSLNKVGIEKFCIVVGRNGMLLMQYLFDRTPT
FVFQDMPKGFGDAVLRAEDFSSNEPFFVHADDGVLTKGYESLKLLFDEVSPDAVLFVRRVENPSRYGVVTIQDKGVYDGH
KLYKVIDAEEKPLHPKSNLAIAAVYIFKPSIFSALKQINVEEGKEIELTYGIHNLLLDGKEVYALEMNEDEKWLNVGDPK
SYLDALNYSFKFL

Sequences:

>Translated_253_residues
MLKKAVITSAGKGSRMKHITSVLPKALLPLFVTENGGKVTRPVIDLIMDSLNKVGIEKFCIVVGRNGMLLMQYLFDRTPT
FVFQDMPKGFGDAVLRAEDFSSNEPFFVHADDGVLTKGYESLKLLFDEVSPDAVLFVRRVENPSRYGVVTIQDKGVYDGH
KLYKVIDAEEKPLHPKSNLAIAAVYIFKPSIFSALKQINVEEGKEIELTYGIHNLLLDGKEVYALEMNEDEKWLNVGDPK
SYLDALNYSFKFL
>Mature_253_residues
MLKKAVITSAGKGSRMKHITSVLPKALLPLFVTENGGKVTRPVIDLIMDSLNKVGIEKFCIVVGRNGMLLMQYLFDRTPT
FVFQDMPKGFGDAVLRAEDFSSNEPFFVHADDGVLTKGYESLKLLFDEVSPDAVLFVRRVENPSRYGVVTIQDKGVYDGH
KLYKVIDAEEKPLHPKSNLAIAAVYIFKPSIFSALKQINVEEGKEIELTYGIHNLLLDGKEVYALEMNEDEKWLNVGDPK
SYLDALNYSFKFL

Specific function: Involved in the biosynthesis of the streptose moiety of streptomycin. Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=255, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790224, Length=252, Percent_Identity=25.3968253968254, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005908
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 28429; Mature: 28429

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKAVITSAGKGSRMKHITSVLPKALLPLFVTENGGKVTRPVIDLIMDSLNKVGIEKFC
CCCCCEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHCCCEEEE
IVVGRNGMLLMQYLFDRTPTFVFQDMPKGFGDAVLRAEDFSSNEPFFVHADDGVLTKGYE
EEECCCCHHHHHHHHCCCCCEEEHHCCCCCCHHEEEECCCCCCCCEEEECCCCCEECCHH
SLKLLFDEVSPDAVLFVRRVENPSRYGVVTIQDKGVYDGHKLYKVIDAEEKPLHPKSNLA
HHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHEEEEECCCCCCCCCCCCEE
IAAVYIFKPSIFSALKQINVEEGKEIELTYGIHNLLLDGKEVYALEMNEDEKWLNVGDPK
EEEEEEECCHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCEEEEEEECCCCCEEECCCHH
SYLDALNYSFKFL
HHHHHHCCCEECC
>Mature Secondary Structure
MLKKAVITSAGKGSRMKHITSVLPKALLPLFVTENGGKVTRPVIDLIMDSLNKVGIEKFC
CCCCCEEECCCCCCHHHHHHHHHHHHHHEEEEECCCCEEHHHHHHHHHHHHHHCCCEEEE
IVVGRNGMLLMQYLFDRTPTFVFQDMPKGFGDAVLRAEDFSSNEPFFVHADDGVLTKGYE
EEECCCCHHHHHHHHCCCCCEEEHHCCCCCCHHEEEECCCCCCCCEEEECCCCCEECCHH
SLKLLFDEVSPDAVLFVRRVENPSRYGVVTIQDKGVYDGHKLYKVIDAEEKPLHPKSNLA
HHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHEEEEECCCCCCCCCCCCEE
IAAVYIFKPSIFSALKQINVEEGKEIELTYGIHNLLLDGKEVYALEMNEDEKWLNVGDPK
EEEEEEECCHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCEEEEEEECCCCCEEECCCHH
SYLDALNYSFKFL
HHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3118332 [H]