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The map label for this gene is lysS [H]

Identifier: 227828292

GI number: 227828292

Start: 1865619

End: 1867097

Strand: Direct

Name: lysS [H]

Synonym: M1425_2033

Alternate gene names: 227828292

Gene position: 1865619-1867097 (Clockwise)

Preceding gene: 227828291

Following gene: 227828294

Centisome position: 71.51

GC content: 34.14

Gene sequence:

>1479_bases
ATGAACTGGGACGAAAGGAGAATAAAAATCGTTGAAGAATTAAGAAAAAATGGCATTGAGCCATATCCTCATAAATACGA
AATAACACATTTGATCAAAGATATTAAGCTTCTTGCATCATCACAAGGTAACAAATCCCATGAGCCATTTATGTTTAATA
TATCTACAGCAGGGCGTGTAGCTAATATCAGAAGGCATGGAAAAGCATCATTTGTAGATATATTTGATGAAGGTGAGAAA
TTACAAATATATATGAGGGTTGATGAGCTTAAAGAGAAATATGACAGATTCTTCACATACGTTGGTAGAGGGGATATAAT
AGGTGTAAGAGGTGATTTATTTTATACCATGAAGGGAGAATTAAGCCTACTCGTAAAGGATTACCAATTATTATCGAAAG
CCCTTATAGAACCTCCAGATTGGTCTAAACTTTCCCCAGAATTTAGATATGCGCATAGATATGTGGATTTCCTCTATAAT
GATAATGCTAGGAAGGCTATGGAAATAAGATATATGATCATAAGAGAAATTAGAGAGTTTCTCTATTCTAAAGGCTTTAT
TGAAGTTGAAACGCCTATTGTTCAACCAGTATATGGTGGGGCATTAGCAAAACCGTTTAAAACACATGTAAATTACTTAA
ACGAAGATTGGTACTTAAGAATAGCATTAGAATTGTACTTAAAAAGGTATATAATAGGCGGATTTAACAAAGTTTTCGAA
ATAGGAAAGGTCTTTAGAAACGAAGATATTGATGTAACTCATAACCCTGAGTTTACTCTCTTAGAGCTCTATTGGGCATA
CGCTGACTACAATGATATAATGAATTTAACTGAAGATCTACTTAAAAGTGTGATCAAAAGGGTTACAAATAGTACTAAGG
TAGCATATGGAAAATATGAAATAGACTTTGAAGCTCCCTTCAAGAGAATCAGTATGTATGACTCTTTATCTGAAATTTTA
GGCAAGAACGTTGAAAATATGAGTGATGATGAACTTAAGGAGCTAATGAAAAAATATAATCTAATTCCTAGAGGAAACCA
GTATGTTAGAGGTCTAATGATAGAGAAATTATTCGATAAACTAGTTACACCCACTTTAACCAACCCAACCTTTATAACTG
ATTATCCAATAGAGACTACACCCCTTTGTAAACCACATAGGAATAAACCTGGACTAGTAGAAAGATTCGAGATGTTCATA
GCAGGCATGGAGATTGCAAATGCGTACACGGAACTGAATGATCCTATATTACAAGATAAACTATTTAGAGAAGAACAAGA
GATGTTTAGAAGAGGAGATGAGGAGGCTCATCCTTACGATAAGGATTTTGTAAGGGCCTTGAGTTATGGAATGCCACCTA
CTGGAGGTTTAGGTATTGGTATAGATAGAATAGTTATGTTAGTGACAAATAATTACAGCATCAAAGAAGTTATTCCTTTC
CCCATGATAAGTAGTAAGGTTATCCTAGAGGACGATTAG

Upstream 100 bases:

>100_bases
AGGAGAGACCCAGTGGTCGCGGGTTCGAGTCCCGCCGGGCCCATACATCTAAACGAAAAAGAAGACAGACTTTAATTTTT
GTCAAGTTATCTAATTGTTG

Downstream 100 bases:

>100_bases
AGAGAAATATTGCAAATATAAAGATCAAGAAGAATAGTGACTGTAAATAATATGAAATTTTTTCGCCGTTACTTTCTCCC
AGTACTCTCTTTAATAATTC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNWDERRIKIVEELRKNGIEPYPHKYEITHLIKDIKLLASSQGNKSHEPFMFNISTAGRVANIRRHGKASFVDIFDEGEK
LQIYMRVDELKEKYDRFFTYVGRGDIIGVRGDLFYTMKGELSLLVKDYQLLSKALIEPPDWSKLSPEFRYAHRYVDFLYN
DNARKAMEIRYMIIREIREFLYSKGFIEVETPIVQPVYGGALAKPFKTHVNYLNEDWYLRIALELYLKRYIIGGFNKVFE
IGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMNLTEDLLKSVIKRVTNSTKVAYGKYEIDFEAPFKRISMYDSLSEIL
GKNVENMSDDELKELMKKYNLIPRGNQYVRGLMIEKLFDKLVTPTLTNPTFITDYPIETTPLCKPHRNKPGLVERFEMFI
AGMEIANAYTELNDPILQDKLFREEQEMFRRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRIVMLVTNNYSIKEVIPF
PMISSKVILEDD

Sequences:

>Translated_492_residues
MNWDERRIKIVEELRKNGIEPYPHKYEITHLIKDIKLLASSQGNKSHEPFMFNISTAGRVANIRRHGKASFVDIFDEGEK
LQIYMRVDELKEKYDRFFTYVGRGDIIGVRGDLFYTMKGELSLLVKDYQLLSKALIEPPDWSKLSPEFRYAHRYVDFLYN
DNARKAMEIRYMIIREIREFLYSKGFIEVETPIVQPVYGGALAKPFKTHVNYLNEDWYLRIALELYLKRYIIGGFNKVFE
IGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMNLTEDLLKSVIKRVTNSTKVAYGKYEIDFEAPFKRISMYDSLSEIL
GKNVENMSDDELKELMKKYNLIPRGNQYVRGLMIEKLFDKLVTPTLTNPTFITDYPIETTPLCKPHRNKPGLVERFEMFI
AGMEIANAYTELNDPILQDKLFREEQEMFRRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRIVMLVTNNYSIKEVIPF
PMISSKVILEDD
>Mature_492_residues
MNWDERRIKIVEELRKNGIEPYPHKYEITHLIKDIKLLASSQGNKSHEPFMFNISTAGRVANIRRHGKASFVDIFDEGEK
LQIYMRVDELKEKYDRFFTYVGRGDIIGVRGDLFYTMKGELSLLVKDYQLLSKALIEPPDWSKLSPEFRYAHRYVDFLYN
DNARKAMEIRYMIIREIREFLYSKGFIEVETPIVQPVYGGALAKPFKTHVNYLNEDWYLRIALELYLKRYIIGGFNKVFE
IGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMNLTEDLLKSVIKRVTNSTKVAYGKYEIDFEAPFKRISMYDSLSEIL
GKNVENMSDDELKELMKKYNLIPRGNQYVRGLMIEKLFDKLVTPTLTNPTFITDYPIETTPLCKPHRNKPGLVERFEMFI
AGMEIANAYTELNDPILQDKLFREEQEMFRRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRIVMLVTNNYSIKEVIPF
PMISSKVILEDD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=41.4829659318637, Blast_Score=351, Evalue=9e-97,
Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=41.4829659318637, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI45439306, Length=458, Percent_Identity=22.9257641921397, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1790571, Length=485, Percent_Identity=42.4742268041237, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789256, Length=485, Percent_Identity=41.2371134020619, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI87082379, Length=321, Percent_Identity=29.9065420560748, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788173, Length=244, Percent_Identity=23.3606557377049, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=498, Percent_Identity=40.1606425702811, Blast_Score=347, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=498, Percent_Identity=40.1606425702811, Blast_Score=347, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=40.1606425702811, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17551876, Length=471, Percent_Identity=22.2929936305732, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=202, Percent_Identity=24.7524752475248, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320242, Length=515, Percent_Identity=40.1941747572816, Blast_Score=333, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6324256, Length=473, Percent_Identity=33.4038054968288, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=379, Percent_Identity=24.802110817942, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640849, Length=500, Percent_Identity=41.2, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24640851, Length=500, Percent_Identity=41.2, Blast_Score=358, Evalue=6e-99,
Organism=Drosophila melanogaster, GI17136276, Length=462, Percent_Identity=22.9437229437229, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24584738, Length=178, Percent_Identity=25.2808988764045, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19921528, Length=453, Percent_Identity=24.9448123620309, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57685; Mature: 57685

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWDERRIKIVEELRKNGIEPYPHKYEITHLIKDIKLLASSQGNKSHEPFMFNISTAGRV
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHH
ANIRRHGKASFVDIFDEGEKLQIYMRVDELKEKYDRFFTYVGRGDIIGVRGDLFYTMKGE
HHHHHCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCC
LSLLVKDYQLLSKALIEPPDWSKLSPEFRYAHRYVDFLYNDNARKAMEIRYMIIREIREF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LYSKGFIEVETPIVQPVYGGALAKPFKTHVNYLNEDWYLRIALELYLKRYIIGGFNKVFE
HHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHH
IGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMNLTEDLLKSVIKRVTNSTKVAYGKYE
HHHHHCCCCCCEECCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IDFEAPFKRISMYDSLSEILGKNVENMSDDELKELMKKYNLIPRGNQYVRGLMIEKLFDK
EECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LVTPTLTNPTFITDYPIETTPLCKPHRNKPGLVERFEMFIAGMEIANAYTELNDPILQDK
HCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LFREEQEMFRRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRIVMLVTNNYSIKEVIPF
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCHHHCCCC
PMISSKVILEDD
CCCCCEEEEECC
>Mature Secondary Structure
MNWDERRIKIVEELRKNGIEPYPHKYEITHLIKDIKLLASSQGNKSHEPFMFNISTAGRV
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHH
ANIRRHGKASFVDIFDEGEKLQIYMRVDELKEKYDRFFTYVGRGDIIGVRGDLFYTMKGE
HHHHHCCCCCEEEEECCCCEEEEEEEHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECCC
LSLLVKDYQLLSKALIEPPDWSKLSPEFRYAHRYVDFLYNDNARKAMEIRYMIIREIREF
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LYSKGFIEVETPIVQPVYGGALAKPFKTHVNYLNEDWYLRIALELYLKRYIIGGFNKVFE
HHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCHHHHHH
IGKVFRNEDIDVTHNPEFTLLELYWAYADYNDIMNLTEDLLKSVIKRVTNSTKVAYGKYE
HHHHHCCCCCCEECCCCEEEEEHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
IDFEAPFKRISMYDSLSEILGKNVENMSDDELKELMKKYNLIPRGNQYVRGLMIEKLFDK
EECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LVTPTLTNPTFITDYPIETTPLCKPHRNKPGLVERFEMFIAGMEIANAYTELNDPILQDK
HCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LFREEQEMFRRGDEEAHPYDKDFVRALSYGMPPTGGLGIGIDRIVMLVTNNYSIKEVIPF
HHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCHHHCCCC
PMISSKVILEDD
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]