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The map label for this gene is tgtA [H]

Identifier: 227828119

GI number: 227828119

Start: 1705540

End: 1707048

Strand: Direct

Name: tgtA [H]

Synonym: M1425_1857

Alternate gene names: 227828119

Gene position: 1705540-1707048 (Clockwise)

Preceding gene: 227828118

Following gene: 227828123

Centisome position: 65.38

GC content: 30.75

Gene sequence:

>1509_bases
ATGACCGTATTTGAAGTGAAATACGAAGATTTGGCAGGAAGAATAGGAATCTTAAAAACAAGAAGCGGTAACTTAGAAAC
TCCAGTATTCTTTCCAGTAATTAATATATTAAAAGATGAAATATCAATAGATGAGATTAAAAATATGGGATTTAAAAATT
TTATCACAAATTCGTATATATTATATAAAAATAAATATATAAAAGATGATATACATAAGGAGCTAAACTCTGAAGATATG
ATCATAATGACGGATTCGGGAGCATATCAAATTCTAGAGTATGGGGACATAGGAATAACCAATTATGAGATAGTGAATTA
TCAGCTCAAGATAAAACCGGACATTGGGGTAATATTAGATTTACCTACCGGAAATACAAATGATTATGAAAATGCTAAAA
TAACAGTATATGAGACTTTAAAGAGAATAGAAGAAGCATCAAAAATCATAGTAAAAAATCAAGATAACAATATTATTTGG
GTATATCCGATACAAGGAGGAAAATATCTTGATCTAGTTAAGACTTCTGCTGAAGGATTATCTAAATTTGAAGACATATA
CAATATGGTTGCGCTTGGTAGTCCAACAGTTCTCTTAGAGCGGTACATGTATGATACCGTAATTGACATGATTTATACTG
CTAAATCTAACATAAAAAGAGGAATTCCGTTTCATTTATTCGGAGGAGGGTTACCTCATATCATTCCATTTGCAGTAGCA
TTAGGAGTAGATAGCTTTGACTCTGCCTCATATATAATATATGCTAGAGATAATAGATATATTACTAGGACACGTGTATA
CAAATTAGAGGATTTAGAATATTTTCCATGTTCTTGTCCAATATGTTCTAAATACACACCTAAAGATTTACTTGAAATGA
ATGAGGAAGAAAGAACAAGAGCATTAGCTATTCATAACCTTTATACTATTTTAGAAGAATTCAAAGCAACTAAACAAGCA
ATTAAGGAAGGAAGACTATTTGAATATCTTCAAGAAAAAGCTTACTCTCATCCTGCAGTATATTCTGCATTCAAAAGATT
GATAAAATATAAAGACTATTTAGAAAAATATGACCCTAGAATAAGGGGAGATCCAAAAGGTTTGTTTTTATTTGATAATA
ACTCTTTACATAGGCCAGAAATCATACGTCACTCGAGATTTTTGGAAAGGTATGTGCAAAAGAAAGATAAAGTAGCTATA
TATTGCTATGATAAGGCGATAAGCGATACTGCCTATGATTATATTAAAAGTATAAAGGAAAGAATAACTGATCATAATAG
TAGTGACATATTTATAGCGATACCGTTTTTTGGTTTAATACCGTTAGAGGTCTCGGACTCTTATCCACTATCTCAATTCG
AGATTCCAAACGAAATAGATGAAGACGTAATAGCCGACATGAAAACTAAAATCGTTTCGTTCTTAAGATGTAAAAATTAC
CAAAAAGTAGAGTTAGTAAACTGTGAAAAACTAGGTTTACATATAGACTCTATCAGCACTTCCTCTTGA

Upstream 100 bases:

>100_bases
AAGTATCACAAAATGGAGTAGAAGGAAGTGGGCCAATGGCCCAAAGAATTTACGATCTATACAACGATTATATAAATAAA
AAGAAAAAGGAATTAGGTCT

Downstream 100 bases:

>100_bases
TAATTCTTTCTGCAATAACTCCATTTGTTTTTGTAAATCCTCTTCTATTCTTTTTGCCTCTTCGTATAATTCATCTACAC
TAACGTTTAAGCTAAGCATT

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTVFEVKYEDLAGRIGILKTRSGNLETPVFFPVINILKDEISIDEIKNMGFKNFITNSYILYKNKYIKDDIHKELNSEDM
IIMTDSGAYQILEYGDIGITNYEIVNYQLKIKPDIGVILDLPTGNTNDYENAKITVYETLKRIEEASKIIVKNQDNNIIW
VYPIQGGKYLDLVKTSAEGLSKFEDIYNMVALGSPTVLLERYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVA
LGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEEERTRALAIHNLYTILEEFKATKQA
IKEGRLFEYLQEKAYSHPAVYSAFKRLIKYKDYLEKYDPRIRGDPKGLFLFDNNSLHRPEIIRHSRFLERYVQKKDKVAI
YCYDKAISDTAYDYIKSIKERITDHNSSDIFIAIPFFGLIPLEVSDSYPLSQFEIPNEIDEDVIADMKTKIVSFLRCKNY
QKVELVNCEKLGLHIDSISTSS

Sequences:

>Translated_502_residues
MTVFEVKYEDLAGRIGILKTRSGNLETPVFFPVINILKDEISIDEIKNMGFKNFITNSYILYKNKYIKDDIHKELNSEDM
IIMTDSGAYQILEYGDIGITNYEIVNYQLKIKPDIGVILDLPTGNTNDYENAKITVYETLKRIEEASKIIVKNQDNNIIW
VYPIQGGKYLDLVKTSAEGLSKFEDIYNMVALGSPTVLLERYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVA
LGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEEERTRALAIHNLYTILEEFKATKQA
IKEGRLFEYLQEKAYSHPAVYSAFKRLIKYKDYLEKYDPRIRGDPKGLFLFDNNSLHRPEIIRHSRFLERYVQKKDKVAI
YCYDKAISDTAYDYIKSIKERITDHNSSDIFIAIPFFGLIPLEVSDSYPLSQFEIPNEIDEDVIADMKTKIVSFLRCKNY
QKVELVNCEKLGLHIDSISTSS
>Mature_501_residues
TVFEVKYEDLAGRIGILKTRSGNLETPVFFPVINILKDEISIDEIKNMGFKNFITNSYILYKNKYIKDDIHKELNSEDMI
IMTDSGAYQILEYGDIGITNYEIVNYQLKIKPDIGVILDLPTGNTNDYENAKITVYETLKRIEEASKIIVKNQDNNIIWV
YPIQGGKYLDLVKTSAEGLSKFEDIYNMVALGSPTVLLERYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVAL
GVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEEERTRALAIHNLYTILEEFKATKQAI
KEGRLFEYLQEKAYSHPAVYSAFKRLIKYKDYLEKYDPRIRGDPKGLFLFDNNSLHRPEIIRHSRFLERYVQKKDKVAIY
CYDKAISDTAYDYIKSIKERITDHNSSDIFIAIPFFGLIPLEVSDSYPLSQFEIPNEIDEDVIADMKTKIVSFLRCKNYQ
KVELVNCEKLGLHIDSISTSS

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1786607, Length=369, Percent_Identity=24.6612466124661, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 58119; Mature: 57988

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVFEVKYEDLAGRIGILKTRSGNLETPVFFPVINILKDEISIDEIKNMGFKNFITNSYI
CCEEEEEHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCEE
LYKNKYIKDDIHKELNSEDMIIMTDSGAYQILEYGDIGITNYEIVNYQLKIKPDIGVILD
EEECCCHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCEEEEEEEEEEECCCCEEEEE
LPTGNTNDYENAKITVYETLKRIEEASKIIVKNQDNNIIWVYPIQGGKYLDLVKTSAEGL
CCCCCCCCCCCCEEEHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCEEEHHHHHHHHH
SKFEDIYNMVALGSPTVLLERYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
LGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEEERTR
HCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHHH
ALAIHNLYTILEEFKATKQAIKEGRLFEYLQEKAYSHPAVYSAFKRLIKYKDYLEKYDPR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
IRGDPKGLFLFDNNSLHRPEIIRHSRFLERYVQKKDKVAIYCYDKAISDTAYDYIKSIKE
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
RITDHNSSDIFIAIPFFGLIPLEVSDSYPLSQFEIPNEIDEDVIADMKTKIVSFLRCKNY
HHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
QKVELVNCEKLGLHIDSISTSS
CEEEEECHHHHCEEEECCCCCC
>Mature Secondary Structure 
TVFEVKYEDLAGRIGILKTRSGNLETPVFFPVINILKDEISIDEIKNMGFKNFITNSYI
CEEEEEHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCEE
LYKNKYIKDDIHKELNSEDMIIMTDSGAYQILEYGDIGITNYEIVNYQLKIKPDIGVILD
EEECCCHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCEEEEEEEEEEECCCCEEEEE
LPTGNTNDYENAKITVYETLKRIEEASKIIVKNQDNNIIWVYPIQGGKYLDLVKTSAEGL
CCCCCCCCCCCCEEEHHHHHHHHHHHCEEEEEECCCCEEEEEECCCCCEEEHHHHHHHHH
SKFEDIYNMVALGSPTVLLERYMYDTVIDMIYTAKSNIKRGIPFHLFGGGLPHIIPFAVA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHH
LGVDSFDSASYIIYARDNRYITRTRVYKLEDLEYFPCSCPICSKYTPKDLLEMNEEERTR
HCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCHHHHHH
ALAIHNLYTILEEFKATKQAIKEGRLFEYLQEKAYSHPAVYSAFKRLIKYKDYLEKYDPR
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
IRGDPKGLFLFDNNSLHRPEIIRHSRFLERYVQKKDKVAIYCYDKAISDTAYDYIKSIKE
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
RITDHNSSDIFIAIPFFGLIPLEVSDSYPLSQFEIPNEIDEDVIADMKTKIVSFLRCKNY
HHCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
QKVELVNCEKLGLHIDSISTSS
CEEEEECHHHHCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]