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The map label for this gene is hisS [H]

Identifier: 227828115

GI number: 227828115

Start: 1702755

End: 1704035

Strand: Direct

Name: hisS [H]

Synonym: M1425_1853

Alternate gene names: 227828115

Gene position: 1702755-1704035 (Clockwise)

Preceding gene: 227828114

Following gene: 227828116

Centisome position: 65.27

GC content: 31.07

Gene sequence:

>1281_bases
ATGACAAAATTTGAAACAGTAAGAGGTATGAAAGACTATATTGGAATCGATGCAGAAAAAATTAGATATTTAGAATCTAC
CTTCAGAGACTTAGCAATAAAATATGGATATTCTGAAATTATAACTCCAGTAGTAGAAGAATTTAAACTGTTTGCGTTGA
AGGGCGGGGAGGAGTTAAGGGAAACCATGTATGTGTTTAAGGATAAGGCAGATAGAGAGTTATCATTAAGGCCTGAAATA
ACACCAAGTGTAGCAAGAGCTTATATACAAAACTTACAGAGTTCACCCAAGCCGATAAGACTATTCTACTTTGGTACCGT
TTATAGATATGATGAACCGCAATATGGCAGATATAGAGAATTTAGACAAGCTGGAATAGAGATGATAGGTGATTCTTCTA
TCTTAGCTGATCTAGAAGTACTAGACTTGTTATATAATTTTTATGATAAGCTTAATCTTTCTAATGATATAACTATTAAA
ATAAATAATATTGGCATATTTAGAAAAATAATGGACAAATATAATATTGAAGATAATTTACAAGAGCACATCTTGCATCT
AATAGATAAGAACAAGATTAACGAAGCTCTAGATATCCTTGAAAAAAACCTAAAGAATAAAGATATAATAGACTTTTTTA
ATAAGATTCTAACTAAGAAGGATACAAAGCTAGAAGATATAGAGTCCCTAGCTGAATTAGAGGAAGTTTCAAGACTAGAT
ATTAAAAGTGAATTTTTATATCTATTTCGATTGTCTAGGATTCTAAGCAACTTAAATATAAAATTTAAGATTGACTTAGG
TTTTGTAAGAGGATTGGCATATTATACTGGATTAATATTTGAGGTTCTTCATCCCTCGGTACAGTTTAGTATTGCCGGAG
GAGGTAGATATGATAAACTTATAGAACTTTATGGTGGCTTACCCTCACCTGCAATAGGATTTGCAATAGGAGTTGAGAGA
ACTTTATTAGTAATTAAAGACTTGAAAGTTGAAGAACCAGTAAATGTAATAGTAATAGGCATGTCAGAAGATACAATTCC
ATCCATGTTTATGGTATCGAGGATATTAAGAAAAGAAGAATATAAGGTAGTAATAAACACTAAAGATCAGCCTCTTTCAA
AGCTATTACCTTACTACGCCTCACAAGGATTTAAAGTCGCGATAATAATAGGTAAGCAAGAATTGGAGAAAAATATGATA
ACTGTTAGAAATTTAATTACACGAAAACAGATTTCCGTTCCACTAGAGAACATTGAAGATGCAATAAAACAAACGTTATA
A

Upstream 100 bases:

>100_bases
GAAATCAATGAATTTAGAACTTTATCTTTCAATTGTTGATAAATATGGGGATGTAACATATTACACAATATCTGAGATAA
AAATGTCAAAGTGACTTAAC

Downstream 100 bases:

>100_bases
ATGGCATTTAGAAGTATATTTATGGGTAAACTAACATGGAAGAATTGCCTGCAACTGCTATTGGTTTAAAGGTAAATGAT
GGAATTGTATTAGCTTCTGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTKFETVRGMKDYIGIDAEKIRYLESTFRDLAIKYGYSEIITPVVEEFKLFALKGGEELRETMYVFKDKADRELSLRPEI
TPSVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYREFRQAGIEMIGDSSILADLEVLDLLYNFYDKLNLSNDITIK
INNIGIFRKIMDKYNIEDNLQEHILHLIDKNKINEALDILEKNLKNKDIIDFFNKILTKKDTKLEDIESLAELEEVSRLD
IKSEFLYLFRLSRILSNLNIKFKIDLGFVRGLAYYTGLIFEVLHPSVQFSIAGGGRYDKLIELYGGLPSPAIGFAIGVER
TLLVIKDLKVEEPVNVIVIGMSEDTIPSMFMVSRILRKEEYKVVINTKDQPLSKLLPYYASQGFKVAIIIGKQELEKNMI
TVRNLITRKQISVPLENIEDAIKQTL

Sequences:

>Translated_426_residues
MTKFETVRGMKDYIGIDAEKIRYLESTFRDLAIKYGYSEIITPVVEEFKLFALKGGEELRETMYVFKDKADRELSLRPEI
TPSVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYREFRQAGIEMIGDSSILADLEVLDLLYNFYDKLNLSNDITIK
INNIGIFRKIMDKYNIEDNLQEHILHLIDKNKINEALDILEKNLKNKDIIDFFNKILTKKDTKLEDIESLAELEEVSRLD
IKSEFLYLFRLSRILSNLNIKFKIDLGFVRGLAYYTGLIFEVLHPSVQFSIAGGGRYDKLIELYGGLPSPAIGFAIGVER
TLLVIKDLKVEEPVNVIVIGMSEDTIPSMFMVSRILRKEEYKVVINTKDQPLSKLLPYYASQGFKVAIIIGKQELEKNMI
TVRNLITRKQISVPLENIEDAIKQTL
>Mature_425_residues
TKFETVRGMKDYIGIDAEKIRYLESTFRDLAIKYGYSEIITPVVEEFKLFALKGGEELRETMYVFKDKADRELSLRPEIT
PSVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYREFRQAGIEMIGDSSILADLEVLDLLYNFYDKLNLSNDITIKI
NNIGIFRKIMDKYNIEDNLQEHILHLIDKNKINEALDILEKNLKNKDIIDFFNKILTKKDTKLEDIESLAELEEVSRLDI
KSEFLYLFRLSRILSNLNIKFKIDLGFVRGLAYYTGLIFEVLHPSVQFSIAGGGRYDKLIELYGGLPSPAIGFAIGVERT
LLVIKDLKVEEPVNVIVIGMSEDTIPSMFMVSRILRKEEYKVVINTKDQPLSKLLPYYASQGFKVAIIIGKQELEKNMIT
VRNLITRKQISVPLENIEDAIKQTL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI15029520, Length=436, Percent_Identity=27.0642201834862, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI6996014, Length=465, Percent_Identity=25.3763440860215, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1788861, Length=438, Percent_Identity=27.8538812785388, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71993686, Length=467, Percent_Identity=23.982869379015, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71993693, Length=467, Percent_Identity=23.982869379015, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325290, Length=457, Percent_Identity=27.1334792122538, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24643061, Length=437, Percent_Identity=24.7139588100687, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24643059, Length=408, Percent_Identity=24.2647058823529, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 49285; Mature: 49154

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKFETVRGMKDYIGIDAEKIRYLESTFRDLAIKYGYSEIITPVVEEFKLFALKGGEELR
CCCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHH
ETMYVFKDKADRELSLRPEITPSVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYRE
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHH
FRQAGIEMIGDSSILADLEVLDLLYNFYDKLNLSNDITIKINNIGIFRKIMDKYNIEDNL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCHH
QEHILHLIDKNKINEALDILEKNLKNKDIIDFFNKILTKKDTKLEDIESLAELEEVSRLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
IKSEFLYLFRLSRILSNLNIKFKIDLGFVRGLAYYTGLIFEVLHPSVQFSIAGGGRYDKL
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
IELYGGLPSPAIGFAIGVERTLLVIKDLKVEEPVNVIVIGMSEDTIPSMFMVSRILRKEE
HHHHCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
YKVVINTKDQPLSKLLPYYASQGFKVAIIIGKQELEKNMITVRNLITRKQISVPLENIED
CEEEEECCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIKQTL
HHHHCC
>Mature Secondary Structure 
TKFETVRGMKDYIGIDAEKIRYLESTFRDLAIKYGYSEIITPVVEEFKLFALKGGEELR
CCCHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHH
ETMYVFKDKADRELSLRPEITPSVARAYIQNLQSSPKPIRLFYFGTVYRYDEPQYGRYRE
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHH
FRQAGIEMIGDSSILADLEVLDLLYNFYDKLNLSNDITIKINNIGIFRKIMDKYNIEDNL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCHH
QEHILHLIDKNKINEALDILEKNLKNKDIIDFFNKILTKKDTKLEDIESLAELEEVSRLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
IKSEFLYLFRLSRILSNLNIKFKIDLGFVRGLAYYTGLIFEVLHPSVQFSIAGGGRYDKL
HHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
IELYGGLPSPAIGFAIGVERTLLVIKDLKVEEPVNVIVIGMSEDTIPSMFMVSRILRKEE
HHHHCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCC
YKVVINTKDQPLSKLLPYYASQGFKVAIIIGKQELEKNMITVRNLITRKQISVPLENIED
CEEEEECCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIKQTL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA