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The map label for this gene is ribH

Identifier: 227827970

GI number: 227827970

Start: 1575914

End: 1576378

Strand: Direct

Name: ribH

Synonym: M1425_1703

Alternate gene names: 227827970

Gene position: 1575914-1576378 (Clockwise)

Preceding gene: 227827969

Following gene: 227827971

Centisome position: 60.41

GC content: 37.85

Gene sequence:

>465_bases
ATGCAGGGGAAATCGATTAGATTAGGAATAGTAGTAGCCGAGTTCAACTACGATATAACGCAATTAATGTTACAAAAAGC
ATTGTCACATGCCAAATTCTTAAACGCTGAGGTGAAAGTAGTTATAAAAGTACCTGGGACATTCGACATGCCTCTTGCAA
TTAAAAAACTGCTTGAGAAAGATTTCATAGACGCCGTAGTGACTTTAGGAGCAGTAATAAAAGGGGAAACTAAACATGAT
GAAATAGTTGCAAGCCAAACTGCTAGGAAAATAGTTGACCTCTCTACAGAATTCAATAAACCTGTGACACTAGGAATAAT
TGGTCATGGAGCAACTCATGAACAAGCTGTTGAAAGAATTGAAGAATACGCAACCAGAGCAGTAGAGGCTGCAATAAAGT
TAGTTCAAAGGACGAGAAAGATAGATGAGCTGAAAGAGGTTAAGGAAACGGTGATTATAGATTGA

Upstream 100 bases:

>100_bases
AGGGCTACTAAACACGCTAAAAACTTAGTTAAGCTCGTAAAGGAAGGTAAAAACGCACTAACTAAATACGCAGGGAAAGG
GTTGAGGCAGGGGTATAATA

Downstream 100 bases:

>100_bases
AGGTATTAGCGATAAAGCTTGTAGATTATAGGGAGTGGACTGAGAGACTAGGATATGATAGGGAATGGTTAATTCAAAAA
ATTCAGAATAAATTCATGAT

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MQGKSIRLGIVVAEFNYDITQLMLQKALSHAKFLNAEVKVVIKVPGTFDMPLAIKKLLEKDFIDAVVTLGAVIKGETKHD
EIVASQTARKIVDLSTEFNKPVTLGIIGHGATHEQAVERIEEYATRAVEAAIKLVQRTRKIDELKEVKETVIID

Sequences:

>Translated_154_residues
MQGKSIRLGIVVAEFNYDITQLMLQKALSHAKFLNAEVKVVIKVPGTFDMPLAIKKLLEKDFIDAVVTLGAVIKGETKHD
EIVASQTARKIVDLSTEFNKPVTLGIIGHGATHEQAVERIEEYATRAVEAAIKLVQRTRKIDELKEVKETVIID
>Mature_154_residues
MQGKSIRLGIVVAEFNYDITQLMLQKALSHAKFLNAEVKVVIKVPGTFDMPLAIKKLLEKDFIDAVVTLGAVIKGETKHD
EIVASQTARKIVDLSTEFNKPVTLGIIGHGATHEQAVERIEEYATRAVEAAIKLVQRTRKIDELKEVKETVIID

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=147, Percent_Identity=32.6530612244898, Blast_Score=65, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324429, Length=151, Percent_Identity=27.8145695364238, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_SULIA (C3MZ62)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843739.1
- GeneID:   7812483
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_1819
- OMA:   AVIQGAT
- ProtClustDB:   PRK00061
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 17091; Mature: 17091

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGKSIRLGIVVAEFNYDITQLMLQKALSHAKFLNAEVKVVIKVPGTFDMPLAIKKLLEK
CCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHH
DFIDAVVTLGAVIKGETKHDEIVASQTARKIVDLSTEFNKPVTLGIIGHGATHEQAVERI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
EEYATRAVEAAIKLVQRTRKIDELKEVKETVIID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQGKSIRLGIVVAEFNYDITQLMLQKALSHAKFLNAEVKVVIKVPGTFDMPLAIKKLLEK
CCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHH
DFIDAVVTLGAVIKGETKHDEIVASQTARKIVDLSTEFNKPVTLGIIGHGATHEQAVERI
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHH
EEYATRAVEAAIKLVQRTRKIDELKEVKETVIID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA