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The map label for this gene is pyrI

Identifier: 227827786

GI number: 227827786

Start: 1418256

End: 1418735

Strand: Direct

Name: pyrI

Synonym: M1425_1519

Alternate gene names: 227827786

Gene position: 1418256-1418735 (Clockwise)

Preceding gene: 227827785

Following gene: 227827787

Centisome position: 54.36

GC content: 32.92

Gene sequence:

>480_bases
ATGATTAGCTCTTCAAAAAGGGATGAATTAATCGTAAGTAAAATAAGGAAAGGTACAGTAATTGATCATATTCCAGCTGG
TAGAGCATTGGCAGTATTGAGAATTCTAGGGATAAGAGGAAGTGAAGGCTATAGAGTAGCGTTAGTTATGAATGTAGAAA
GTAAAAAAATAGGAAGGAAAGATATAGTGAAAATTGAGGATAGGGTAATTGACGAGAAAGAAGCTAGCCTAATAACACTA
ATAGCTCCATCTGCTACCATAAATATTATAAGAGATTACGTAGTGACAGAGAAAAGGCATTTAGAGGTTCCAAAGCAGAT
TAGAGGATTAATAAAATGTCCTAATCCACAGTGTATAACAAATAATGACGTTGAGGCTGAAAGTAGATTTACAACAATTT
CAATTAAACCTCTTAAGCTAAAGTGTGAATATTGTGAGATATATATTACAGAAGAAGATGTCATAAGGCAGATATTATGA

Upstream 100 bases:

>100_bases
AAAGTCGATAAAACTACAAACGCTAAATACTTCTATCAAGCATCATTAGCCGTTCCAGTTAGAATGGCTTTATTTTATGA
GGTTTTGGGTGAAAGGAAAG

Downstream 100 bases:

>100_bases
TATATTATGAAGTTATTGAAAAGAAAATTATAGATAATGTGTACGAAATAAAAATAGCCAACTTTAACGTTAAACCAAAA
GCAGGCCAATACGTGAGTCT

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MISSSKRDELIVSKIRKGTVIDHIPAGRALAVLRILGIRGSEGYRVALVMNVESKKIGRKDIVKIEDRVIDEKEASLITL
IAPSATINIIRDYVVTEKRHLEVPKQIRGLIKCPNPQCITNNDVEAESRFTTISIKPLKLKCEYCEIYITEEDVIRQIL

Sequences:

>Translated_159_residues
MISSSKRDELIVSKIRKGTVIDHIPAGRALAVLRILGIRGSEGYRVALVMNVESKKIGRKDIVKIEDRVIDEKEASLITL
IAPSATINIIRDYVVTEKRHLEVPKQIRGLIKCPNPQCITNNDVEAESRFTTISIKPLKLKCEYCEIYITEEDVIRQIL
>Mature_159_residues
MISSSKRDELIVSKIRKGTVIDHIPAGRALAVLRILGIRGSEGYRVALVMNVESKKIGRKDIVKIEDRVIDEKEASLITL
IAPSATINIIRDYVVTEKRHLEVPKQIRGLIKCPNPQCITNNDVEAESRFTTISIKPLKLKCEYCEIYITEEDVIRQIL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=139, Percent_Identity=34.5323741007194, Blast_Score=94, Evalue=4e-21,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_SULIA (C3N689)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002843559.1
- GeneID:   7814625
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_1634
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17981; Mature: 17981

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSSKRDELIVSKIRKGTVIDHIPAGRALAVLRILGIRGSEGYRVALVMNVESKKIGRK
CCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHCCCH
DIVKIEDRVIDEKEASLITLIAPSATINIIRDYVVTEKRHLEVPKQIRGLIKCPNPQCIT
HHEEHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHCCCCHHHHHHEECCCCCEEC
NNDVEAESRFTTISIKPLKLKCEYCEIYITEEDVIRQIL
CCCCCCCCEEEEEEEEEEEEEEEEEEEEECHHHHHHHHC
>Mature Secondary Structure
MISSSKRDELIVSKIRKGTVIDHIPAGRALAVLRILGIRGSEGYRVALVMNVESKKIGRK
CCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCHHCCCH
DIVKIEDRVIDEKEASLITLIAPSATINIIRDYVVTEKRHLEVPKQIRGLIKCPNPQCIT
HHEEHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHCCHHHCCCCHHHHHHEECCCCCEEC
NNDVEAESRFTTISIKPLKLKCEYCEIYITEEDVIRQIL
CCCCCCCCEEEEEEEEEEEEEEEEEEEEECHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA