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The map label for this gene is pyrB [H]

Identifier: 227827785

GI number: 227827785

Start: 1417352

End: 1418263

Strand: Direct

Name: pyrB [H]

Synonym: M1425_1518

Alternate gene names: 227827785

Gene position: 1417352-1418263 (Clockwise)

Preceding gene: 227827782

Following gene: 227827786

Centisome position: 54.33

GC content: 35.53

Gene sequence:

>912_bases
GTGAGATTAAGACACGTAGTTTCTTCCCTAGACCTGACCAGAGATGACTATTTTCGAATATTCGAACTTGCAGACAAGTT
CTCTAATGTGAAAAAACTAAATTATCTATCAGGGAAAGTAGTTTCATTAGCTTTCTTTGAGCCAAGTACTAGAACTGCTC
AAAGCTTTCATACCGCAGCAATAAAATTGGGTGCTGATGTAATAGGATTTGCCTCAGAAGAATCTACTTCGATAGCTAAG
GGTGAAAATCTAGCTGATACCATTAGGATGTTGAACAATTACTCAAATTGTATTGTAATGAGACATAAGTTTGATGGAGC
AGCGTTATTTGCTAGTGAGATAAGTGATATACCTATAATCAATGCCGGAGATGGAAAGCACGAGCATCCCACCCAAGCAC
TCATAGATTTATATACTATTTATAAGGTGTTTGGCGAAATAGATGGGAGAACTTTCGGATTATTAGGGGATCTGAAATAC
GCAAGGACCGTAAACAGTTTGCTAAGAGCATTAACTAGATTCAAACCAAAGAAGATATTTCTAATTTCTCCTTCTCAGTT
AAAGGTCAGAAGAGAGATTTTAGATGGATTAAACTATCCAGTTATAGAAACTGAAAATCCCTATGACGTGATACAAGATA
TCGACGTGTTATATGTGACTCGAATTCAAAAAGAAAGATTTGTTGATGAATTAGAATACGAGAAAGTTAAGGAGAGTTAT
GTGGTTGATCTCAAGTTAGTTAATATGATGAAGAAAGATGGAATTATATTGCATCCATTACCTAGGGTTACCGAAATAGA
TAGGAAAGTCGATAAAACTACAAACGCTAAATACTTCTATCAAGCATCATTAGCCGTTCCAGTTAGAATGGCTTTATTTT
ATGAGGTTTTGGGTGAAAGGAAAGATGATTAG

Upstream 100 bases:

>100_bases
ATAATTTCCTTTCGAGTAATACTTCTGCGAAACTCATACGTAGAGAGTCTGTGAAAAAGAATTTAAATATACTTCTATCA
TAACCAATTATAAGGGCTTT

Downstream 100 bases:

>100_bases
CTCTTCAAAAAGGGATGAATTAATCGTAAGTAAAATAAGGAAAGGTACAGTAATTGATCATATTCCAGCTGGTAGAGCAT
TGGCAGTATTGAGAATTCTA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRLRHVVSSLDLTRDDYFRIFELADKFSNVKKLNYLSGKVVSLAFFEPSTRTAQSFHTAAIKLGADVIGFASEESTSIAK
GENLADTIRMLNNYSNCIVMRHKFDGAALFASEISDIPIINAGDGKHEHPTQALIDLYTIYKVFGEIDGRTFGLLGDLKY
ARTVNSLLRALTRFKPKKIFLISPSQLKVRREILDGLNYPVIETENPYDVIQDIDVLYVTRIQKERFVDELEYEKVKESY
VVDLKLVNMMKKDGIILHPLPRVTEIDRKVDKTTNAKYFYQASLAVPVRMALFYEVLGERKDD

Sequences:

>Translated_303_residues
MRLRHVVSSLDLTRDDYFRIFELADKFSNVKKLNYLSGKVVSLAFFEPSTRTAQSFHTAAIKLGADVIGFASEESTSIAK
GENLADTIRMLNNYSNCIVMRHKFDGAALFASEISDIPIINAGDGKHEHPTQALIDLYTIYKVFGEIDGRTFGLLGDLKY
ARTVNSLLRALTRFKPKKIFLISPSQLKVRREILDGLNYPVIETENPYDVIQDIDVLYVTRIQKERFVDELEYEKVKESY
VVDLKLVNMMKKDGIILHPLPRVTEIDRKVDKTTNAKYFYQASLAVPVRMALFYEVLGERKDD
>Mature_303_residues
MRLRHVVSSLDLTRDDYFRIFELADKFSNVKKLNYLSGKVVSLAFFEPSTRTAQSFHTAAIKLGADVIGFASEESTSIAK
GENLADTIRMLNNYSNCIVMRHKFDGAALFASEISDIPIINAGDGKHEHPTQALIDLYTIYKVFGEIDGRTFGLLGDLKY
ARTVNSLLRALTRFKPKKIFLISPSQLKVRREILDGLNYPVIETENPYDVIQDIDVLYVTRIQKERFVDELEYEKVKESY
VVDLKLVNMMKKDGIILHPLPRVTEIDRKVDKTTNAKYFYQASLAVPVRMALFYEVLGERKDD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=301, Percent_Identity=38.2059800664452, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI2367364, Length=299, Percent_Identity=42.809364548495, Blast_Score=231, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI193204318, Length=319, Percent_Identity=40.7523510971787, Blast_Score=203, Evalue=8e-53,
Organism=Saccharomyces cerevisiae, GI6322331, Length=305, Percent_Identity=40.655737704918, Blast_Score=216, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6322373, Length=291, Percent_Identity=26.8041237113402, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24642586, Length=305, Percent_Identity=38.3606557377049, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34632; Mature: 34632

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRHVVSSLDLTRDDYFRIFELADKFSNVKKLNYLSGKVVSLAFFEPSTRTAQSFHTAA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
IKLGADVIGFASEESTSIAKGENLADTIRMLNNYSNCIVMRHKFDGAALFASEISDIPII
HHHCCHHEECCCCCCCCHHCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCEE
NAGDGKHEHPTQALIDLYTIYKVFGEIDGRTFGLLGDLKYARTVNSLLRALTRFKPKKIF
ECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
LISPSQLKVRREILDGLNYPVIETENPYDVIQDIDVLYVTRIQKERFVDELEYEKVKESY
EECCHHHHHHHHHHCCCCCCEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDLKLVNMMKKDGIILHPLPRVTEIDRKVDKTTNAKYFYQASLAVPVRMALFYEVLGER
HHHHHHHHHHHHCCEEECCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCC
KDD
CCC
>Mature Secondary Structure
MRLRHVVSSLDLTRDDYFRIFELADKFSNVKKLNYLSGKVVSLAFFEPSTRTAQSFHTAA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
IKLGADVIGFASEESTSIAKGENLADTIRMLNNYSNCIVMRHKFDGAALFASEISDIPII
HHHCCHHEECCCCCCCCHHCCCCHHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCCCEE
NAGDGKHEHPTQALIDLYTIYKVFGEIDGRTFGLLGDLKYARTVNSLLRALTRFKPKKIF
ECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
LISPSQLKVRREILDGLNYPVIETENPYDVIQDIDVLYVTRIQKERFVDELEYEKVKESY
EECCHHHHHHHHHHCCCCCCEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VVDLKLVNMMKKDGIILHPLPRVTEIDRKVDKTTNAKYFYQASLAVPVRMALFYEVLGER
HHHHHHHHHHHHCCEEECCCCCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCC
KDD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA