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The map label for this gene is gatA [H]

Identifier: 227827542

GI number: 227827542

Start: 1208412

End: 1209836

Strand: Direct

Name: gatA [H]

Synonym: M1425_1269

Alternate gene names: 227827542

Gene position: 1208412-1209836 (Clockwise)

Preceding gene: 227827541

Following gene: 227827543

Centisome position: 46.32

GC content: 35.58

Gene sequence:

>1425_bases
ATGATAGTGGAATTAGTCAATGATTTGAAAAATAAGAATTTAGAGGCTGAAGAATACGTTGAGAGAACTTATGAGAAAAT
AGGCAAATACGATAAGTACACCAACGCGTTTATAACTATTAGAAGCAAGGAAGAGATATTAAGGGAAGTAAAAGAAAACA
TTAACAAAGGGGGCAAATTAGCAGGGATACTAATTGCCATCAAGGATAACATCTCAACCAAGGGAATTAGAACTACTTGT
GCATCAAAGATGTTAGAAGATTACATACCTCCATATGATGCTACAGTTATAGAAAAATTAAAGAAGGAAGGAGCGGTAAT
TGTTGGTAAAACGAATATGGACGAATTCGCTATGGGTTCAACTACGGAGACGAGTTATTTTGGTCCCACTAGAAATCCTT
GGAATTTAGAAAGAACCCCTGGAGGTTCATCTGGAGGTAGTGGAGCAGCTCTAGCAGCTGGTTATGTCGAATTGGCGTTA
GGTAGTGATACTGGAGGTTCTATAAGAGCGCCAGCTGCATATAATGCAACTTTTGGTTTAAAGCCATCTTATGGCACAGT
GAGTAGATTTGGTCTAGTAGCTTATGCAAATAGTTTAGAACAAATAGGGCCTATGGCTAGAAATGCTGAAGACTTAGGAT
TGCTTTTCTCAATAATTGCTGGACCTGACGATAAGGACGCTACTACACTTGACTTATCTCTAAATTTTGAGTTTAAGGAA
CAGAATGTGAAAAGCGTTAGGATAGGTATTCTTAGTGATATTTTAGAAATGTCAGAAAAACCAGTATCTGGTGTGATAAA
AGATGTTATAGATAAACTATCTAGTGAAGGAGCTCTAATTGAAGATACCAAATTAGGTAATGCAGAATACGCACTACCAG
CATACTACATAATAGCAATGTCTGAAGCAAGCTCTAATTTAGCTAGATATGATGGAGTTAGGTACGGATATAGCAAATAC
ATGGAAGGGAATTGGCGTGAGGTTTATGCTAAAAATAGAGGAGAAGCGTTTGGTATAGAGGTAAAGAGGAGGATCCTCCT
TGGTAGTTTTATCTTAAGTGCTGGATATTATGAGGAATTTTACCTCAAGGCATTAAAGGTTAGAAATTTAATTAAGAAAA
GTCTAGACGAATTATTCACAAAATATGATATTTTAGTCTCTCCTACAATGCCTATATTGCCGCCTAAAATAGGTGAGGTA
ATCAATGACCCTGTGAGAATGTATGCAATGGATCTAAATACTGTAATAGCTAACTTAGCTGCAATACCAGCATTATCATT
GCCAGCGGGATTTTATAATGACTTACCAATAGGATTACAATTAATGGGAAAATATCTATCAGATATTTATTTAATTAACA
TTTCATCCTATATAGAGAGAAATGTTACAAAATTGTACAATTTAACAGCACCAATTAAGATCTAA

Upstream 100 bases:

>100_bases
ACTCCAAGAGATCCATTAGATAGGGAAAATGCACTCAAAAACGTGAAAAGAAAAGAAAACGGATATATAGTAGGACCTAG
AACTTACGGTGAATAAGATT

Downstream 100 bases:

>100_bases
TAATTTTTTAATCCTATATGCGGTAGAATGTACAATATGCAAAGTAATTTAAGGATCGATACAGTAAGTGAGATATTTAA
AGAGTTACAAAAAGAGAAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIVELVNDLKNKNLEAEEYVERTYEKIGKYDKYTNAFITIRSKEEILREVKENINKGGKLAGILIAIKDNISTKGIRTTC
ASKMLEDYIPPYDATVIEKLKKEGAVIVGKTNMDEFAMGSTTETSYFGPTRNPWNLERTPGGSSGGSGAALAAGYVELAL
GSDTGGSIRAPAAYNATFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDDKDATTLDLSLNFEFKE
QNVKSVRIGILSDILEMSEKPVSGVIKDVIDKLSSEGALIEDTKLGNAEYALPAYYIIAMSEASSNLARYDGVRYGYSKY
MEGNWREVYAKNRGEAFGIEVKRRILLGSFILSAGYYEEFYLKALKVRNLIKKSLDELFTKYDILVSPTMPILPPKIGEV
INDPVRMYAMDLNTVIANLAAIPALSLPAGFYNDLPIGLQLMGKYLSDIYLINISSYIERNVTKLYNLTAPIKI

Sequences:

>Translated_474_residues
MIVELVNDLKNKNLEAEEYVERTYEKIGKYDKYTNAFITIRSKEEILREVKENINKGGKLAGILIAIKDNISTKGIRTTC
ASKMLEDYIPPYDATVIEKLKKEGAVIVGKTNMDEFAMGSTTETSYFGPTRNPWNLERTPGGSSGGSGAALAAGYVELAL
GSDTGGSIRAPAAYNATFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDDKDATTLDLSLNFEFKE
QNVKSVRIGILSDILEMSEKPVSGVIKDVIDKLSSEGALIEDTKLGNAEYALPAYYIIAMSEASSNLARYDGVRYGYSKY
MEGNWREVYAKNRGEAFGIEVKRRILLGSFILSAGYYEEFYLKALKVRNLIKKSLDELFTKYDILVSPTMPILPPKIGEV
INDPVRMYAMDLNTVIANLAAIPALSLPAGFYNDLPIGLQLMGKYLSDIYLINISSYIERNVTKLYNLTAPIKI
>Mature_474_residues
MIVELVNDLKNKNLEAEEYVERTYEKIGKYDKYTNAFITIRSKEEILREVKENINKGGKLAGILIAIKDNISTKGIRTTC
ASKMLEDYIPPYDATVIEKLKKEGAVIVGKTNMDEFAMGSTTETSYFGPTRNPWNLERTPGGSSGGSGAALAAGYVELAL
GSDTGGSIRAPAAYNATFGLKPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDDKDATTLDLSLNFEFKE
QNVKSVRIGILSDILEMSEKPVSGVIKDVIDKLSSEGALIEDTKLGNAEYALPAYYIIAMSEASSNLARYDGVRYGYSKY
MEGNWREVYAKNRGEAFGIEVKRRILLGSFILSAGYYEEFYLKALKVRNLIKKSLDELFTKYDILVSPTMPILPPKIGEV
INDPVRMYAMDLNTVIANLAAIPALSLPAGFYNDLPIGLQLMGKYLSDIYLINISSYIERNVTKLYNLTAPIKI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=36.6733466933868, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI195972892, Length=487, Percent_Identity=24.8459958932238, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI166795287, Length=209, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=462, Percent_Identity=35.9307359307359, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=472, Percent_Identity=25.635593220339, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=166, Percent_Identity=32.5301204819277, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=265, Percent_Identity=28.3018867924528, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=422, Percent_Identity=38.1516587677725, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6319685, Length=440, Percent_Identity=33.1818181818182, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6320448, Length=169, Percent_Identity=34.9112426035503, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=431, Percent_Identity=40.6032482598608, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24652985, Length=422, Percent_Identity=28.1990521327014, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922090, Length=422, Percent_Identity=28.1990521327014, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24652981, Length=422, Percent_Identity=28.1990521327014, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24652983, Length=422, Percent_Identity=28.1990521327014, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45550774, Length=237, Percent_Identity=32.9113924050633, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=237, Percent_Identity=32.9113924050633, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24648437, Length=237, Percent_Identity=32.9113924050633, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI161078093, Length=492, Percent_Identity=27.4390243902439, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648441, Length=176, Percent_Identity=35.7954545454545, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648439, Length=176, Percent_Identity=35.7954545454545, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=241, Percent_Identity=29.4605809128631, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356731, Length=250, Percent_Identity=27.6, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52228; Mature: 52228

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVELVNDLKNKNLEAEEYVERTYEKIGKYDKYTNAFITIRSKEEILREVKENINKGGKL
CHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCE
AGILIAIKDNISTKGIRTTCASKMLEDYIPPYDATVIEKLKKEGAVIVGKTNMDEFAMGS
EEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCHHCCCC
TTETSYFGPTRNPWNLERTPGGSSGGSGAALAAGYVELALGSDTGGSIRAPAAYNATFGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCEECCCCCCCCCCC
KPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDDKDATTLDLSLNFEFKE
CCCCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECC
QNVKSVRIGILSDILEMSEKPVSGVIKDVIDKLSSEGALIEDTKLGNAEYALPAYYIIAM
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEE
SEASSNLARYDGVRYGYSKYMEGNWREVYAKNRGEAFGIEVKRRILLGSFILSAGYYEEF
CCCCCCHHHHCCCHHCHHHHCCCCHHHHHCCCCCCEECHHHHHHHHHHHHHHHCCCHHHH
YLKALKVRNLIKKSLDELFTKYDILVSPTMPILPPKIGEVINDPVRMYAMDLNTVIANLA
HHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
AIPALSLPAGFYNDLPIGLQLMGKYLSDIYLINISSYIERNVTKLYNLTAPIKI
HCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIVELVNDLKNKNLEAEEYVERTYEKIGKYDKYTNAFITIRSKEEILREVKENINKGGKL
CHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHCCCCCE
AGILIAIKDNISTKGIRTTCASKMLEDYIPPYDATVIEKLKKEGAVIVGKTNMDEFAMGS
EEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCCHHCCCC
TTETSYFGPTRNPWNLERTPGGSSGGSGAALAAGYVELALGSDTGGSIRAPAAYNATFGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCEECCCCCCCCCCC
KPSYGTVSRFGLVAYANSLEQIGPMARNAEDLGLLFSIIAGPDDKDATTLDLSLNFEFKE
CCCCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECC
QNVKSVRIGILSDILEMSEKPVSGVIKDVIDKLSSEGALIEDTKLGNAEYALPAYYIIAM
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEE
SEASSNLARYDGVRYGYSKYMEGNWREVYAKNRGEAFGIEVKRRILLGSFILSAGYYEEF
CCCCCCHHHHCCCHHCHHHHCCCCHHHHHCCCCCCEECHHHHHHHHHHHHHHHCCCHHHH
YLKALKVRNLIKKSLDELFTKYDILVSPTMPILPPKIGEVINDPVRMYAMDLNTVIANLA
HHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
AIPALSLPAGFYNDLPIGLQLMGKYLSDIYLINISSYIERNVTKLYNLTAPIKI
HCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]