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The map label for this gene is hycD [H]

Identifier: 227827476

GI number: 227827476

Start: 1131194

End: 1132147

Strand: Direct

Name: hycD [H]

Synonym: M1425_1198

Alternate gene names: 227827476

Gene position: 1131194-1132147 (Clockwise)

Preceding gene: 227827467

Following gene: 227827477

Centisome position: 43.36

GC content: 37.84

Gene sequence:

>954_bases
ATGGCGCCAAATGAAGCTCAGATAGTAGTTTTAACCATAACTCAAGTTATTGTAGTTATTCTACTATCCCCATTATATCA
AGGAATTTACGATAGGGGAAAAGCCATAATAGAAGGGCGTAAAGGCCCCAGTGTTCTGCAACCTTACTATGATATAATTA
AACTACTAAGAAAAGAAACCGTTATTTCAAATAACTCATTATCTCTATTTGTTTATGCTCCTTACATAGTTTTTGGGATT
TATCTCCTTATTTCCTTCGTCATCCCAGTAGTTTATCCAGTGCCCATCTTACTCACCCCTACGGTGGATTTCTTAGGGGG
AGCATTACTCTTCTCCTTAGCTGCGTTCATAAAAATAATTGCCTCCCTAGAAAGTGGAAGTAATTTCGTAGCATTAGGAG
TAAGTAGAATTCTCTCTTTCACTTTCTTATCTGAAGCTACACTGATAACGGTTTTCTTCGGCGTTGCACTAATAACTGGT
ACAAACAACCCTTACGTAACATTAGATTACGTTTCTCAAAGCCTCTCTCATTACTTTCAACTAGACCACATCTTCGTATC
GATCTCATTCTTCATGTTATGGTTATTCGAAACCGGTAAATTACCCGTAGAGAGTCCTGGACTAAGCGAAATGGGTATGA
TAGACGATGGAGTTCTCTACGAATATAGCGGAAAATTACTTGCAATACTAAAATGGGGTTCTTACATCAAACAATATCTA
TTAGGCTCAGTACTCCTAAACGTATTTATATTCCCTTGGTTTTTGCAAGTTGGAATTATAGGCTCTTTAATAGACATTGC
GGTGATGTTCGGCAAATGGTTACTCCTAATCCTTATTAGCGTTATAATAAACACCACCCTAGCTAAATTAAGGCTATTTA
AAGTTCAGGATTATCTCGCAGTGGCGTTCCTCCTTTCCCTACTTTCATTAACGCTTACTGTCTTGTTGGGGTGA

Upstream 100 bases:

>100_bases
TGAAGTAGGGGATGGCGTCCCCCTGGGGATAAGCCCGAACCGCCTCTTGATTAATGATAGGGGCTGATGACGCCTACTTC
ACCACAAAAGGGTGGGAAGT

Downstream 100 bases:

>100_bases
AGGTATAGTGATTAACTCAGAGATCCTAGAACTACTCTCAACGTTCATATTAATTTCAGCCTTTTACATTCAAGGACAAG
CTTACTTTAAACCAGCAATA

Product: formate hydrogenlyase subunit 4 (HycD)

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIVFGI
YLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITG
TNNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQYL
LGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG

Sequences:

>Translated_317_residues
MAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIVFGI
YLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITG
TNNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQYL
LGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG
>Mature_316_residues
APNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKETVISNNSLSLFVYAPYIVFGIY
LLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKIIASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITGT
NNPYVTLDYVSQSLSHYFQLDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQYLL
GSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLAVAFLLSLLSLTLTVLLG

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=288, Percent_Identity=32.6388888888889, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082115, Length=254, Percent_Identity=27.1653543307087, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 35120; Mature: 34988

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKET
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
VISNNSLSLFVYAPYIVFGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKII
HCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHH
ASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITGTNNPYVTLDYVSQSLSHYFQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHH
LDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQYL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHH
LGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFLLSLLSLTLTVLLG
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APNEAQIVVLTITQVIVVILLSPLYQGIYDRGKAIIEGRKGPSVLQPYYDIIKLLRKET
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHH
VISNNSLSLFVYAPYIVFGIYLLISFVIPVVYPVPILLTPTVDFLGGALLFSLAAFIKII
HCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHH
ASLESGSNFVALGVSRILSFTFLSEATLITVFFGVALITGTNNPYVTLDYVSQSLSHYFQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEHHHHHHHHHHHHH
LDHIFVSISFFMLWLFETGKLPVESPGLSEMGMIDDGVLYEYSGKLLAILKWGSYIKQYL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHH
LGSVLLNVFIFPWFLQVGIIGSLIDIAVMFGKWLLLILISVIINTTLAKLRLFKVQDYLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAFLLSLLSLTLTVLLG
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]