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The map label for this gene is tuf [H]

Identifier: 227826628

GI number: 227826628

Start: 190757

End: 192328

Strand: Direct

Name: tuf [H]

Synonym: M1425_0219

Alternate gene names: 227826628

Gene position: 190757-192328 (Clockwise)

Preceding gene: 227826626

Following gene: 227826631

Centisome position: 7.31

GC content: 36.58

Gene sequence:

>1572_bases
ATGAAATTACCCCGAGAAAATGAAGAAGGAAAAGTAGAATATAAGCTTATTCTTTCTGGCGTGACGCCAGAGCGTCTTCA
AGAATTAGCTTCTCAAATGAAGTACAGATTAGAGGAAGGTTATGGGGAAGCATTTTACGTTATAGGTGTAAGTAATGAGG
GGGAAATAATAGGGTTATCCAAGAGTGAATTAGAGGAAAGTATAGCTACTCTAGACATGATTGCTAAGCTAGTAAACGCT
AAAATTGTGTATAGAAGAGACGTTGAAGTAAGAAAGGATAAATACGTGGCTGAACTTCTAGTTAGGAGATATAAGGAGAA
TTTACCAGTGGAGGTCAACGTTGCAGTAATGGGTCATGTAAATGCAGGGAAAAGTACTGTAACTGGTGCGTTGGTTCTAG
GAAGATTGGATGATGGAAATGGAGGTTTAAGAACAGCAATTGCAAGGCATCTCCATGAAGTTCTATCTGGTAGAACATCT
TCCATTACATTGAGACTGATCGGATTTGATGATAGCGGTAAGATAGTGAATTGGCAATTAAAGGATCCACTTGACGAGGC
GGAAACAACGATTAAGAGTACTAAGATAGTTAGACTTATTGATCTTGGAGGGCATGAGAGATACCTTAGAACCACTCTAA
AAGGATTATTAGGTTATGAGGTAAATTACGTTATGCTAGTAGTTGGAGCTGATGATGGTTTAAGTATAATGGGCAAAGAA
CATTTAGCCTTAGCTTCAATATTAAAATTTCCTATATTTGTAGTTATTACTAAGGTAGATAAATATCCAGAAGATAGGAT
AAAAGGCATAGTTAATGATATTAAAAACGTTTTAAAAATTCCCGGAATTAATAGATTGGCTCTAGAAGTTGAGGATGAGG
ATGATGTTGTAAATTCAATTTTGGCTATAAAGACTAAGCGTGTAGTTCCTATTTTTAGAATATCGAATGTAAGTGGAAAA
GGTTTAGATTTGCTTATAAAATTACTAAACCTATTACCTCCGGAGGCTAAGAACTTAGATGAGAAAGAGACACCACTAGT
TTATATCGATGAAGCATATAACGTAACTGGAGTGGGTACTGTTGTACTTGGTTCCGTTGTGAGAGGTAAGCTTAGGAGTA
ATGAGTCTGTGTTAATTGGGCCTAATAAGTTAGGAGAGTTTAAGGAGGTTAAGATAAAGAGTATACAAGTAAATAAGGTA
TTTGTGGATTCCGTTTTGGCTGGGAATATAGCCACATTTGCTATTCAAGGTATTGAAAAGGAGAGTCTTAGAAAAGGAAT
GGTAATGGTGAAGGGAATACCTAAGGTAGTTAGAAGATTTAAGGCGAGAGTGTTTATACTTCACCATCCAACTACCATTA
GAGAAGGTTATGTTGCTACCCTTCACTCTTATACAATAAGGCAAGCGGTAAAGTTTGAGAGAATTCAGAGTGGGTTTCTA
AGAACTGGCGATACGAGTGAAATAGTATTGCATTTTCTGTATAGGCCAGAATACTTAGAGAAGGGACAAATTTTCGTATT
CAGAGAAGGTAGAACTAGAGGTGTAGGTATTGTATTGGAACCTATTGGTTAA

Upstream 100 bases:

>100_bases
TCTTCAAATATTACCTTCGGTCCCTCAGACATTAACAATCCCCTATTAAAATTTATTTAGTTGTTCACAAATTTAATACT
TGTTAAAGTGATGTGATGTA

Downstream 100 bases:

>100_bases
TTTTCCCTACACTACACTCTATAATCTGCTTTGATATTTCCTCTGCTTGTGAACCTATATGCTTCTTTAAACTATTTGCT
AATGGTCCGCCCACTTCATA

Product: protein synthesis factor GTP-binding

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKLPRENEEGKVEYKLILSGVTPERLQELASQMKYRLEEGYGEAFYVIGVSNEGEIIGLSKSELEESIATLDMIAKLVNA
KIVYRRDVEVRKDKYVAELLVRRYKENLPVEVNVAVMGHVNAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTS
SITLRLIGFDDSGKIVNWQLKDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
HLALASILKFPIFVVITKVDKYPEDRIKGIVNDIKNVLKIPGINRLALEVEDEDDVVNSILAIKTKRVVPIFRISNVSGK
GLDLLIKLLNLLPPEAKNLDEKETPLVYIDEAYNVTGVGTVVLGSVVRGKLRSNESVLIGPNKLGEFKEVKIKSIQVNKV
FVDSVLAGNIATFAIQGIEKESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFERIQSGFL
RTGDTSEIVLHFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG

Sequences:

>Translated_523_residues
MKLPRENEEGKVEYKLILSGVTPERLQELASQMKYRLEEGYGEAFYVIGVSNEGEIIGLSKSELEESIATLDMIAKLVNA
KIVYRRDVEVRKDKYVAELLVRRYKENLPVEVNVAVMGHVNAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTS
SITLRLIGFDDSGKIVNWQLKDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
HLALASILKFPIFVVITKVDKYPEDRIKGIVNDIKNVLKIPGINRLALEVEDEDDVVNSILAIKTKRVVPIFRISNVSGK
GLDLLIKLLNLLPPEAKNLDEKETPLVYIDEAYNVTGVGTVVLGSVVRGKLRSNESVLIGPNKLGEFKEVKIKSIQVNKV
FVDSVLAGNIATFAIQGIEKESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFERIQSGFL
RTGDTSEIVLHFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG
>Mature_523_residues
MKLPRENEEGKVEYKLILSGVTPERLQELASQMKYRLEEGYGEAFYVIGVSNEGEIIGLSKSELEESIATLDMIAKLVNA
KIVYRRDVEVRKDKYVAELLVRRYKENLPVEVNVAVMGHVNAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTS
SITLRLIGFDDSGKIVNWQLKDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
HLALASILKFPIFVVITKVDKYPEDRIKGIVNDIKNVLKIPGINRLALEVEDEDDVVNSILAIKTKRVVPIFRISNVSGK
GLDLLIKLLNLLPPEAKNLDEKETPLVYIDEAYNVTGVGTVVLGSVVRGKLRSNESVLIGPNKLGEFKEVKIKSIQVNKV
FVDSVLAGNIATFAIQGIEKESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFERIQSGFL
RTGDTSEIVLHFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG5258

COG function: function code R; GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI82546879, Length=508, Percent_Identity=40.748031496063, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI45593140, Length=531, Percent_Identity=39.3596986817326, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI4503475, Length=402, Percent_Identity=24.8756218905473, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI4503471, Length=431, Percent_Identity=24.5939675174014, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI34147630, Length=423, Percent_Identity=26.0047281323877, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI2367247, Length=238, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17565438, Length=533, Percent_Identity=36.9606003752345, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI17563834, Length=535, Percent_Identity=37.3831775700935, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17552884, Length=421, Percent_Identity=24.9406175771971, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17569207, Length=421, Percent_Identity=24.9406175771971, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=426, Percent_Identity=25.3521126760563, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI32566303, Length=411, Percent_Identity=24.0875912408759, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=426, Percent_Identity=23.943661971831, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922512, Length=531, Percent_Identity=38.6064030131827, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24654852, Length=531, Percent_Identity=38.6064030131827, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI28573213, Length=531, Percent_Identity=37.2881355932203, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI221378008, Length=531, Percent_Identity=37.2881355932203, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24644462, Length=531, Percent_Identity=37.2881355932203, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24644464, Length=531, Percent_Identity=37.2881355932203, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24644466, Length=531, Percent_Identity=37.2881355932203, Blast_Score=335, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24652838, Length=428, Percent_Identity=24.7663551401869, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137572, Length=428, Percent_Identity=24.7663551401869, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45553807, Length=413, Percent_Identity=25.4237288135593, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI45553816, Length=413, Percent_Identity=25.4237288135593, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24651721, Length=413, Percent_Identity=25.4237288135593, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI17864154, Length=413, Percent_Identity=25.4237288135593, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281363316, Length=422, Percent_Identity=24.6445497630332, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17864358, Length=422, Percent_Identity=24.6445497630332, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI45550900, Length=388, Percent_Identity=26.0309278350515, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 58278; Mature: 58278

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPRENEEGKVEYKLILSGVTPERLQELASQMKYRLEEGYGEAFYVIGVSNEGEIIGLS
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECC
KSELEESIATLDMIAKLVNAKIVYRRDVEVRKDKYVAELLVRRYKENLPVEVNVAVMGHV
HHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEC
NAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTSSITLRLIGFDDSGKIVNWQL
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEE
KDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
CCCHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEECCHH
HLALASILKFPIFVVITKVDKYPEDRIKGIVNDIKNVLKIPGINRLALEVEDEDDVVNSI
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
LAIKTKRVVPIFRISNVSGKGLDLLIKLLNLLPPEAKNLDEKETPLVYIDEAYNVTGVGT
HHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHH
VVLGSVVRGKLRSNESVLIGPNKLGEFKEVKIKSIQVNKVFVDSVLAGNIATFAIQGIEK
HHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCEEEHHHHHCCEEEEEEECCCH
ESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFERIQSGFL
HHHHCCCEEECCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
RTGDTSEIVLHFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG
CCCCHHHHHHHHHHCCCHHCCCCEEEEECCCCCCEEEEEECCC
>Mature Secondary Structure
MKLPRENEEGKVEYKLILSGVTPERLQELASQMKYRLEEGYGEAFYVIGVSNEGEIIGLS
CCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECC
KSELEESIATLDMIAKLVNAKIVYRRDVEVRKDKYVAELLVRRYKENLPVEVNVAVMGHV
HHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEC
NAGKSTVTGALVLGRLDDGNGGLRTAIARHLHEVLSGRTSSITLRLIGFDDSGKIVNWQL
CCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEE
KDPLDEAETTIKSTKIVRLIDLGGHERYLRTTLKGLLGYEVNYVMLVVGADDGLSIMGKE
CCCHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCEECCHH
HLALASILKFPIFVVITKVDKYPEDRIKGIVNDIKNVLKIPGINRLALEVEDEDDVVNSI
HHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
LAIKTKRVVPIFRISNVSGKGLDLLIKLLNLLPPEAKNLDEKETPLVYIDEAYNVTGVGT
HHHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHH
VVLGSVVRGKLRSNESVLIGPNKLGEFKEVKIKSIQVNKVFVDSVLAGNIATFAIQGIEK
HHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCEEEHHHHHCCEEEEEEECCCH
ESLRKGMVMVKGIPKVVRRFKARVFILHHPTTIREGYVATLHSYTIRQAVKFERIQSGFL
HHHHCCCEEECCHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
RTGDTSEIVLHFLYRPEYLEKGQIFVFREGRTRGVGIVLEPIG
CCCCHHHHHHHHHHCCCHHCCCCEEEEECCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA