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The map label for this gene is rnhB [H]

Identifier: 227826588

GI number: 227826588

Start: 155106

End: 155744

Strand: Direct

Name: rnhB [H]

Synonym: M1425_0178

Alternate gene names: 227826588

Gene position: 155106-155744 (Clockwise)

Preceding gene: 227826587

Following gene: 227826589

Centisome position: 5.95

GC content: 36.93

Gene sequence:

>639_bases
ATGAGAATAGGTATAGACGAAGCAGGTCGTGGAGCCTTAATAGGTCCAATGATAGTTGCAGGTGTAGCTATCAGTGATTC
AAAGCTAAAATTCCTTAAAGGCATTGGAGTAAAGGATAGTAAGCAATTAACTAGGGAGAGAAGGGAGAAATTGTTCGATA
TTATTGCCAGTACCGTAGATGCTTTCACAGTAGTTAAAGTTTTTCCTTACGAAATAGATAATTACAACTTGAACGATCTC
ACATATGATGCAGTGTCTAAGATAATTCTATCACTTTCGGTTTTCAATCCTGAAATAGTAACAGTTGATAAAGTGGGAGA
AGAGAAGCCTGTTATAGAGCTCATCAGAAAGCTAGGTTATAAGTCAAATGTAGTTCACAAGGCGGATGTCCTATTTGTTG
AAGCAAGTGCAGCAAGCATTATTGCTAAAGTAATAAGGGATAATTATATTGATGAGTTGAAAAAAGTTTACGGTGATTTC
GGGAGTGGATACCCTGCTGATCCTAGAACAATAAAGTGGCTTAAGTCCTTTTATGAGAAGAACCCAAATCCACCTCCCAT
TGTCAGAAGATCATGGAAAATTTTACGTTCTACTGCTCCACTATATTATATAAGTAAAGAGGGTAGAAGACTATGGTAA

Upstream 100 bases:

>100_bases
TGATATCTTCTATTGTTGACGTTGAAGTTAATGAGAGTTATCAGAATGAAAAGATGAAAAACGGTAAAAGGGAGGCAAAA
CTAGTTATTTCATTGAGGAT

Downstream 100 bases:

>100_bases
CGAATTTACCCGCTGAAGCCAAGGCTAAATGGCTAAAGGTGATGGACGCAAAGACACCAGAGGAGAAATTCCAAGCTTTG
CAGGAATTTCTTAAGGAAGT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRIGIDEAGRGALIGPMIVAGVAISDSKLKFLKGIGVKDSKQLTRERREKLFDIIASTVDAFTVVKVFPYEIDNYNLNDL
TYDAVSKIILSLSVFNPEIVTVDKVGEEKPVIELIRKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKKVYGDF
GSGYPADPRTIKWLKSFYEKNPNPPPIVRRSWKILRSTAPLYYISKEGRRLW

Sequences:

>Translated_212_residues
MRIGIDEAGRGALIGPMIVAGVAISDSKLKFLKGIGVKDSKQLTRERREKLFDIIASTVDAFTVVKVFPYEIDNYNLNDL
TYDAVSKIILSLSVFNPEIVTVDKVGEEKPVIELIRKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKKVYGDF
GSGYPADPRTIKWLKSFYEKNPNPPPIVRRSWKILRSTAPLYYISKEGRRLW
>Mature_212_residues
MRIGIDEAGRGALIGPMIVAGVAISDSKLKFLKGIGVKDSKQLTRERREKLFDIIASTVDAFTVVKVFPYEIDNYNLNDL
TYDAVSKIILSLSVFNPEIVTVDKVGEEKPVIELIRKLGYKSNVVHKADVLFVEASAASIIAKVIRDNYIDELKKVYGDF
GSGYPADPRTIKWLKSFYEKNPNPPPIVRRSWKILRSTAPLYYISKEGRRLW

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Homo sapiens, GI38455391, Length=214, Percent_Identity=34.5794392523364, Blast_Score=103, Evalue=8e-23,
Organism=Escherichia coli, GI1786380, Length=166, Percent_Identity=33.1325301204819, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536263, Length=215, Percent_Identity=34.8837209302326, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324257, Length=226, Percent_Identity=34.5132743362832, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24580635, Length=212, Percent_Identity=33.0188679245283, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 23924; Mature: 23924

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDEAGRGALIGPMIVAGVAISDSKLKFLKGIGVKDSKQLTRERREKLFDIIASTVD
CCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFTVVKVFPYEIDNYNLNDLTYDAVSKIILSLSVFNPEIVTVDKVGEEKPVIELIRKLGY
HHHHHHEECEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCC
KSNVVHKADVLFVEASAASIIAKVIRDNYIDELKKVYGDFGSGYPADPRTIKWLKSFYEK
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
NPNPPPIVRRSWKILRSTAPLYYISKEGRRLW
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure
MRIGIDEAGRGALIGPMIVAGVAISDSKLKFLKGIGVKDSKQLTRERREKLFDIIASTVD
CCCCCCCCCCCCEEHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AFTVVKVFPYEIDNYNLNDLTYDAVSKIILSLSVFNPEIVTVDKVGEEKPVIELIRKLGY
HHHHHHEECEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCC
KSNVVHKADVLFVEASAASIIAKVIRDNYIDELKKVYGDFGSGYPADPRTIKWLKSFYEK
CCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
NPNPPPIVRRSWKILRSTAPLYYISKEGRRLW
CCCCCHHHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]