Click here to switch to the map view.

The map label for this gene is moeA [C]

Identifier: 227826573

GI number: 227826573

Start: 142897

End: 144537

Strand: Direct

Name: moeA [C]

Synonym: M1425_0163

Alternate gene names: 227826573

Gene position: 142897-144537 (Clockwise)

Preceding gene: 227826572

Following gene: 227826574

Centisome position: 5.48

GC content: 34.49

Gene sequence:

>1641_bases
ATGAGAGTGTTTGTTGGAGACGAATCATTATATTCAATAGATGAGGCAATAAAAGTCTACTTATCATCATTAAATTTCAC
TCCTAAAATCGTAAAAGTAGAAGTAAAGGACTCTTTCGGTTACGTATCTGCAGAAGACCTTATGTCGCCTATTGATTATC
CTCCATTTTCAAGATCAAACGTAGATGGTTACGCCCTAAAATCTTCATGCACGCCTGGAGAGCTAAAAGTAATAGATAGA
ATAGGAATTGGAGAGTTTAAAGAAATTCACATAAAAGAATGTGTGGCAGTTGAAGTAGATACTGGTGCAATGATTCCAAT
GGGTGCAGATGCTGTAATAAAAGTTGAAAATGTCAAACTAATCAATGGTAATTTCATAAAAATAGACAAAAAGATTAACT
TTGGCCAAAATATTGGGTGGATAGGGAGTGATATTCCTAAAAACTCAATTATATTGAGAAAAGGCGAAGTAATTTCTCAT
GAAAAGATTGGTTTGCTAGCCTCATTAGGGATAAGTAGTATAAAAGTTTATGAAAAGTTAAAGATATATTTAATCGCGAC
GGGAGATGAGCTAGTTGAACCTGGTAACTCCTTGTCTCCAGGTAAAATTTACGAGTCTAATCTACACTACTTATATTCTA
AGCTCAAAGAAAATTACAAAATAGTCGGTCTTTCATTACTTAGCGATGATATAAGGAGTATCAAGAATGAGATAAAGAGG
GCTATCTCAGTTGCTGATGTTTTGATACTGACTGGCGGTACTAGCGCTGGGGAAAAAGATTTCGTTCATAGAGCTATAAG
AGAGTTAGGCAATATAATAGTTCATGGTATAAAGATTAAACCTGGAAAGCCTACCATTTTAGGAATCGTAGATGGTAAGC
CAGTAATTGGATTACCTGGGAATATAGTATCTTCAATGGTTGTATTGAATATGGTTATTTTGGAAATTCTGAAAAGTCTT
TATCCTTCTCGTAAAGAAATACTTGGGTTAGGTAAAATTAAAGCTAGATTAGCACTACGCGTTAAAGCTGATGAACATAG
AAATACTCTGATTCCAGTTTATTTATTTAGGAGTATTGATAATTCCTATTATGCTCTTCCAGTAAAGTTCGATAGCTATA
TGGTAGGTACTTTTTCCTTAACTGAGGGTTATATAATGCTTGGACCTAACGAAGAAATTGAAGAAGGTAAGGAAGTCGAG
GTTGATGTGAAGAAGTTTGATGATTCGATATCTATTATAGGAGAGGAGGATAAGAGAATCCTTAATTTAGATACAAAAAA
TGTGTTGCTTGGTTCCTTACCCGCAAGTAAAGCTATTGAATATAAATTTGGGGACGTTGCAGTTATTAGTTCATTATTTA
CTGAACCCATAGAGAATTATGATAAAGTACTCTGTAGACAAATTCTAGTTAATGGTGAGGGAGAGGAAATAGGCTATGAT
GATTGGATTGGGATGTCTAAAATTGTTAAGAACCCGGTAGTGAAGTTAAAATCTCCTTCTACAATTTACTCGTTAGTAGG
TAAGGCAAGGGTTTATGCTCCAGAAGGTTATATAGTAGGTGATAAGGTAGCTGAGGAATGTTTATACATGATAGGAATTA
GTGAGAGGGGTAAGAGGTATCTAAGTACAATAAAAGTATAG

Upstream 100 bases:

>100_bases
AGATATATCGCAAGTTCGTGATCCCGTAGTAATGGTATTAGACGAGTTAGAAAAGGCGTCTCTCATAGCTTTTTAATCGT
ATAAGATATTGTTTTTTTAT

Downstream 100 bases:

>100_bases
TACTTAACTAATAATAATATGAGAATACTGGACGTAAAATAATTAGAGTAATACTCTAAACTACTTAAAGATTTAACATA
TAATGTGTTCTTGATCTGAG

Product: molybdopterin biosynthesis protein MoeA/unknown domain fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MRVFVGDESLYSIDEAIKVYLSSLNFTPKIVKVEVKDSFGYVSAEDLMSPIDYPPFSRSNVDGYALKSSCTPGELKVIDR
IGIGEFKEIHIKECVAVEVDTGAMIPMGADAVIKVENVKLINGNFIKIDKKINFGQNIGWIGSDIPKNSIILRKGEVISH
EKIGLLASLGISSIKVYEKLKIYLIATGDELVEPGNSLSPGKIYESNLHYLYSKLKENYKIVGLSLLSDDIRSIKNEIKR
AISVADVLILTGGTSAGEKDFVHRAIRELGNIIVHGIKIKPGKPTILGIVDGKPVIGLPGNIVSSMVVLNMVILEILKSL
YPSRKEILGLGKIKARLALRVKADEHRNTLIPVYLFRSIDNSYYALPVKFDSYMVGTFSLTEGYIMLGPNEEIEEGKEVE
VDVKKFDDSISIIGEEDKRILNLDTKNVLLGSLPASKAIEYKFGDVAVISSLFTEPIENYDKVLCRQILVNGEGEEIGYD
DWIGMSKIVKNPVVKLKSPSTIYSLVGKARVYAPEGYIVGDKVAEECLYMIGISERGKRYLSTIKV

Sequences:

>Translated_546_residues
MRVFVGDESLYSIDEAIKVYLSSLNFTPKIVKVEVKDSFGYVSAEDLMSPIDYPPFSRSNVDGYALKSSCTPGELKVIDR
IGIGEFKEIHIKECVAVEVDTGAMIPMGADAVIKVENVKLINGNFIKIDKKINFGQNIGWIGSDIPKNSIILRKGEVISH
EKIGLLASLGISSIKVYEKLKIYLIATGDELVEPGNSLSPGKIYESNLHYLYSKLKENYKIVGLSLLSDDIRSIKNEIKR
AISVADVLILTGGTSAGEKDFVHRAIRELGNIIVHGIKIKPGKPTILGIVDGKPVIGLPGNIVSSMVVLNMVILEILKSL
YPSRKEILGLGKIKARLALRVKADEHRNTLIPVYLFRSIDNSYYALPVKFDSYMVGTFSLTEGYIMLGPNEEIEEGKEVE
VDVKKFDDSISIIGEEDKRILNLDTKNVLLGSLPASKAIEYKFGDVAVISSLFTEPIENYDKVLCRQILVNGEGEEIGYD
DWIGMSKIVKNPVVKLKSPSTIYSLVGKARVYAPEGYIVGDKVAEECLYMIGISERGKRYLSTIKV
>Mature_546_residues
MRVFVGDESLYSIDEAIKVYLSSLNFTPKIVKVEVKDSFGYVSAEDLMSPIDYPPFSRSNVDGYALKSSCTPGELKVIDR
IGIGEFKEIHIKECVAVEVDTGAMIPMGADAVIKVENVKLINGNFIKIDKKINFGQNIGWIGSDIPKNSIILRKGEVISH
EKIGLLASLGISSIKVYEKLKIYLIATGDELVEPGNSLSPGKIYESNLHYLYSKLKENYKIVGLSLLSDDIRSIKNEIKR
AISVADVLILTGGTSAGEKDFVHRAIRELGNIIVHGIKIKPGKPTILGIVDGKPVIGLPGNIVSSMVVLNMVILEILKSL
YPSRKEILGLGKIKARLALRVKADEHRNTLIPVYLFRSIDNSYYALPVKFDSYMVGTFSLTEGYIMLGPNEEIEEGKEVE
VDVKKFDDSISIIGEEDKRILNLDTKNVLLGSLPASKAIEYKFGDVAVISSLFTEPIENYDKVLCRQILVNGEGEEIGYD
DWIGMSKIVKNPVVKLKSPSTIYSLVGKARVYAPEGYIVGDKVAEECLYMIGISERGKRYLSTIKV

Specific function: Involved In The Biosynthesis Of A Demolybdo-Cofactor (Molybdopterin), Necessary For Molybdo-Enzymes. Seems To Be Involved In A Step Of Activation Of Molybdenum In The Form Of Thiomolybdenum. [C]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI66932975, Length=414, Percent_Identity=31.1594202898551, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI10880983, Length=414, Percent_Identity=31.1594202898551, Blast_Score=169, Evalue=5e-42,
Organism=Escherichia coli, GI1787049, Length=387, Percent_Identity=29.9741602067183, Blast_Score=166, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25147659, Length=334, Percent_Identity=30.2395209580838, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17562606, Length=315, Percent_Identity=26.6666666666667, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136978, Length=282, Percent_Identity=32.2695035460993, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24638841, Length=282, Percent_Identity=32.2695035460993, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 60282; Mature: 60282

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFVGDESLYSIDEAIKVYLSSLNFTPKIVKVEVKDSFGYVSAEDLMSPIDYPPFSRSN
CEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHCCCCCCCCCCCCC
VDGYALKSSCTPGELKVIDRIGIGEFKEIHIKECVAVEVDTGAMIPMGADAVIKVENVKL
CCCEEEECCCCCCCEEEEEECCCCCCHHEEEEEEEEEEECCCEECCCCCCEEEEEEEEEE
INGNFIKIDKKINFGQNIGWIGSDIPKNSIILRKGEVISHEKIGLLASLGISSIKVYEKL
ECCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHEEEEEEE
KIYLIATGDELVEPGNSLSPGKIYESNLHYLYSKLKENYKIVGLSLLSDDIRSIKNEIKR
EEEEEECCHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHH
AISVADVLILTGGTSAGEKDFVHRAIRELGNIIVHGIKIKPGKPTILGIVDGKPVIGLPG
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCEEECCH
NIVSSMVVLNMVILEILKSLYPSRKEILGLGKIKARLALRVKADEHRNTLIPVYLFRSID
HHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEEEEECCCCCCCEEEEEEEECCC
NSYYALPVKFDSYMVGTFSLTEGYIMLGPNEEIEEGKEVEVDVKKFDDSISIIGEEDKRI
CCEEEEEEEECCEEEEEEEECCCEEEECCCCHHCCCCEEEEEHHHCCCCEEEEECCCCEE
LNLDTKNVLLGSLPASKAIEYKFGDVAVISSLFTEPIENYDKVLCRQILVNGEGEEIGYD
EECCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
DWIGMSKIVKNPVVKLKSPSTIYSLVGKARVYAPEGYIVGDKVAEECLYMIGISERGKRY
HHCCHHHHHCCCCEEECCCHHHHHHHCCCEEECCCCEEECHHHHHHHHHEECCCHHHHHH
LSTIKV
HHHCCC
>Mature Secondary Structure
MRVFVGDESLYSIDEAIKVYLSSLNFTPKIVKVEVKDSFGYVSAEDLMSPIDYPPFSRSN
CEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEHHHHCCCCCCCCCCCCC
VDGYALKSSCTPGELKVIDRIGIGEFKEIHIKECVAVEVDTGAMIPMGADAVIKVENVKL
CCCEEEECCCCCCCEEEEEECCCCCCHHEEEEEEEEEEECCCEECCCCCCEEEEEEEEEE
INGNFIKIDKKINFGQNIGWIGSDIPKNSIILRKGEVISHEKIGLLASLGISSIKVYEKL
ECCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCHHEEEEEEE
KIYLIATGDELVEPGNSLSPGKIYESNLHYLYSKLKENYKIVGLSLLSDDIRSIKNEIKR
EEEEEECCHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHH
AISVADVLILTGGTSAGEKDFVHRAIRELGNIIVHGIKIKPGKPTILGIVDGKPVIGLPG
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCEEEEEECCCCEEECCH
NIVSSMVVLNMVILEILKSLYPSRKEILGLGKIKARLALRVKADEHRNTLIPVYLFRSID
HHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEEEEECCCCCCCEEEEEEEECCC
NSYYALPVKFDSYMVGTFSLTEGYIMLGPNEEIEEGKEVEVDVKKFDDSISIIGEEDKRI
CCEEEEEEEECCEEEEEEEECCCEEEECCCCHHCCCCEEEEEHHHCCCCEEEEECCCCEE
LNLDTKNVLLGSLPASKAIEYKFGDVAVISSLFTEPIENYDKVLCRQILVNGEGEEIGYD
EECCCCCEEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
DWIGMSKIVKNPVVKLKSPSTIYSLVGKARVYAPEGYIVGDKVAEECLYMIGISERGKRY
HHCCHHHHHCCCCEEECCCHHHHHHHCCCEEECCCCEEECHHHHHHHHHEECCCHHHHHH
LSTIKV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]