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The map label for this gene is thrB

Identifier: 227826574

GI number: 227826574

Start: 144876

End: 145811

Strand: Direct

Name: thrB

Synonym: M1425_0164

Alternate gene names: 227826574

Gene position: 144876-145811 (Clockwise)

Preceding gene: 227826573

Following gene: 227826575

Centisome position: 5.55

GC content: 37.82

Gene sequence:

>936_bases
GTGGAATGTAAAAGAGCTAGGGCATATTCGAGTTCAGCTAATTTAGGTTCAGGCTTTGATATATTATCAATGGCGCATAC
TGCGTTTTTTGACACTGTCGAAATATGCGTTGAGACTAAGAATTCAGAAAATATTGTGATCGAAAGTAATTCAAAAATTC
CGCTTGAACCAAATAGGAACTCGGCTACTTATCCATTAGTTAGAATTATGGAAGAAAGAGGGATAAAGGCTAGTCTTAGA
GTTAAAGTGATAAAGGGTATTCCGGAGGGTTTAGGATTAGGGAGTAGCGGAGCGTCGGCAACTGCAGCTGTTATGGCGTT
TAGTAGTTTATTTAATCTAAACTTATCTAAGGAGGACCTAGTTAGGTATGCGATGTACGGGGAGATCGCTTCTTCTGGGT
CTCCTCATCCAGATAATGTCGCCGCTAGTGTTTTCGGCGGAGTTGTCTCCGTAGTTTCCGTTAATCCGGTTAAGGTTGTT
GAAATTCCTTTAAATTACTCATTTAATATTTTGCTTTTCGTCCCATTAAATGTACATATTGAAGAGAAAACTAAGAAGGC
CAGAGAAATGGTACCTAAAACAGTTAAACTTTCTGATTATATAAATAATTCAAGATATATCTCCTCTTTGCTAATTGGCT
TCGTTAAAGGAGAGAGGGATCTGATAAGATTAGGCTTAAATGACGAAATTGTGGAGAAAGCTCGACTTCCCTTATTCCCA
TACTATCCTAAAATTAAAGAAATCGCGATAAAATACGACGCAGTAGGATCTTGTGTAAGTGGCGCGGGTCCTTCAATCTT
AGTTTTGACCGATAAAATGACCGATGAAAATAAAATTGCAGAAGAGGGTACTAAAACTTGTAATGAATTTAATGTTGAAT
GTGAAGTTATCAAAGCTAAAATTGCTGGAGGTGTAGAGGTTGAAAGACGCAACTAG

Upstream 100 bases:

>100_bases
CTTATTAGATAGTATAATTTGTTTTATATCATCAAAATCTATCTTATGTAGCCTCTTACAAGATAATAAGAAATTTATAA
GTATTATTATTATTTAGGTT

Downstream 100 bases:

>100_bases
AACAGTAAGGGAAGATATAGATGAAGCGACTGGTGCTATTACTACTCCAATATATCAGACAACTGCCTATCATTATCCTG
AAGGAGAGAAATACAGATAT

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MECKRARAYSSSANLGSGFDILSMAHTAFFDTVEICVETKNSENIVIESNSKIPLEPNRNSATYPLVRIMEERGIKASLR
VKVIKGIPEGLGLGSSGASATAAVMAFSSLFNLNLSKEDLVRYAMYGEIASSGSPHPDNVAASVFGGVVSVVSVNPVKVV
EIPLNYSFNILLFVPLNVHIEEKTKKAREMVPKTVKLSDYINNSRYISSLLIGFVKGERDLIRLGLNDEIVEKARLPLFP
YYPKIKEIAIKYDAVGSCVSGAGPSILVLTDKMTDENKIAEEGTKTCNEFNVECEVIKAKIAGGVEVERRN

Sequences:

>Translated_311_residues
MECKRARAYSSSANLGSGFDILSMAHTAFFDTVEICVETKNSENIVIESNSKIPLEPNRNSATYPLVRIMEERGIKASLR
VKVIKGIPEGLGLGSSGASATAAVMAFSSLFNLNLSKEDLVRYAMYGEIASSGSPHPDNVAASVFGGVVSVVSVNPVKVV
EIPLNYSFNILLFVPLNVHIEEKTKKAREMVPKTVKLSDYINNSRYISSLLIGFVKGERDLIRLGLNDEIVEKARLPLFP
YYPKIKEIAIKYDAVGSCVSGAGPSILVLTDKMTDENKIAEEGTKTCNEFNVECEVIKAKIAGGVEVERRN
>Mature_311_residues
MECKRARAYSSSANLGSGFDILSMAHTAFFDTVEICVETKNSENIVIESNSKIPLEPNRNSATYPLVRIMEERGIKASLR
VKVIKGIPEGLGLGSSGASATAAVMAFSSLFNLNLSKEDLVRYAMYGEIASSGSPHPDNVAASVFGGVVSVVSVNPVKVV
EIPLNYSFNILLFVPLNVHIEEKTKKAREMVPKTVKLSDYINNSRYISSLLIGFVKGERDLIRLGLNDEIVEKARLPLFP
YYPKIKEIAIKYDAVGSCVSGAGPSILVLTDKMTDENKIAEEGTKTCNEFNVECEVIKAKIAGGVEVERRN

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=281, Percent_Identity=29.1814946619217, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321814, Length=340, Percent_Identity=25, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_SULIA (C3N0N3)

Other databases:

- EMBL:   CP001401
- RefSeq:   YP_002842239.1
- ProteinModelPortal:   C3N0N3
- GeneID:   7813857
- GenomeReviews:   CP001401_GR
- KEGG:   sim:M1627_0164
- OMA:   HYSACAK
- ProtClustDB:   PRK01212
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 33887; Mature: 33887

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECKRARAYSSSANLGSGFDILSMAHTAFFDTVEICVETKNSENIVIESNSKIPLEPNRN
CCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCCCCCCCC
SATYPLVRIMEERGIKASLRVKVIKGIPEGLGLGSSGASATAAVMAFSSLFNLNLSKEDL
CCCHHHHHHHHHCCCEEEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
VRYAMYGEIASSGSPHPDNVAASVFGGVVSVVSVNPVKVVEIPLNYSFNILLFVPLNVHI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEEECCCCEEEEEEEEEEEEE
EEKTKKAREMVPKTVKLSDYINNSRYISSLLIGFVKGERDLIRLGLNDEIVEKARLPLFP
CHHHHHHHHHCCCEEEHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCC
YYPKIKEIAIKYDAVGSCVSGAGPSILVLTDKMTDENKIAEEGTKTCNEFNVECEVIKAK
CCCCHHHEEEEHHHHCHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEE
IAGGVEVERRN
CCCCEEEEECC
>Mature Secondary Structure
MECKRARAYSSSANLGSGFDILSMAHTAFFDTVEICVETKNSENIVIESNSKIPLEPNRN
CCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCCCEEEEECCCCCCCCCCCC
SATYPLVRIMEERGIKASLRVKVIKGIPEGLGLGSSGASATAAVMAFSSLFNLNLSKEDL
CCCHHHHHHHHHCCCEEEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
VRYAMYGEIASSGSPHPDNVAASVFGGVVSVVSVNPVKVVEIPLNYSFNILLFVPLNVHI
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEECCCEEEEEEECCCCEEEEEEEEEEEEE
EEKTKKAREMVPKTVKLSDYINNSRYISSLLIGFVKGERDLIRLGLNDEIVEKARLPLFP
CHHHHHHHHHCCCEEEHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCC
YYPKIKEIAIKYDAVGSCVSGAGPSILVLTDKMTDENKIAEEGTKTCNEFNVECEVIKAK
CCCCHHHEEEEHHHHCHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEEE
IAGGVEVERRN
CCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA