Click here to switch to the map view.

The map label for this gene is leuB

Identifier: 227815751

GI number: 227815751

Start: 2892863

End: 2893927

Strand: Reverse

Name: leuB

Synonym: BAMEG_3174

Alternate gene names: 227815751

Gene position: 2893927-2892863 (Counterclockwise)

Preceding gene: 227815752

Following gene: 227815750

Centisome position: 55.33

GC content: 42.63

Gene sequence:

>1065_bases
TTGGAAAAACGTATCGTTTGTTTAGCTGGTGATGGTGTTGGCCCAGAAGTTATGGAAAGTGCGAAGGAAGTATTGCATAT
GGTAGAGAGGCTATATGGACATCATTTTCATTTGCAAGATGAGCATTTTGGTGGTGTTGCTATCGATTTAACGGGGCAAC
CATTACCACAACGAACACTTGCAGCTTGTTTAGCAAGTGATGCGGTTTTACTAGGCGCAGTTGGTGGACCAAGGTGGGAT
GGTGCGAAAGAAAGGCCAGAGAAAGGATTATTAGCTTTAAGAAAAGGGCTTGGTGTATTTGCGAACGTCCGCCCTGTCAC
GGTAGAAAGTGCAACGGCACATTTATCGCCATTGAAAAAGGCTGATGAAATTGATTTCGTTGTCGTTCGTGAATTAACAG
GTGGGATTTATTTTTCTTATCCGAAAGAACGAACGGACGAAGTAGCAACAGATACACTTACGTATCATCGCCATGAAATT
GAACGTATCGTTTCTTGCGCCTTTCAATTAGCGAGTAAGCGGAAGAAAAAAGTAACATCTATTGATAAGGCGAATGTTTT
AGAATCTAGTAAACTATGGAGAATTGTAACAGAAGAAGTAGCACTTCGTTATCCAGATGTTGAACTAGAACATATTTTAG
TAGATGCGGCCGCTATGGAGCTAATTCGGAATCCAGGACGGTTTGATGTAATTGTAACAGAAAATTTATTTGGGGATATT
TTAAGTGACGAAGCTTCCGTATTAGCTGGATCATTAGGGATGCTTCCGTCAGCAAGTCACGCGGAAAAGGGACCTTCCTT
ATATGAGCCTATTCACGGATCGGCACCGGATATTGCCGGGAAAAATAAGGCGAATCCAATTGCTATGATGCGCTCTGTTG
CCATGATGCTTGGGCAATCGTTCGGATTAACGAGGGAAGGGTGTGCAATTGAAGAAGCGATTTCAGCAGTCCTTAAATCA
GGAAAATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACAAAGGCAGTTATGCAAGAAATGGAAGAACA
AGCGCTAGTAGGGAGAGGAAGATAA

Upstream 100 bases:

>100_bases
CTTTTATCCAGCTTGTGAAATAAGTGTTTGATAGGTGAGAGGAGCTATTCTCGCCTATCAAATTAAAGTTCTGAAAATTC
GGTTAAAAGGAGTGTTGAAA

Downstream 100 bases:

>100_bases
TGGGGAAAAGGTTGCTAGATAAGCTTTGGGAAAGACACGTAGTTACGACCAACGAAAATGGATTGGATTTATTATATATC
GATCTGCATCTTGTTCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSCAFQLASKRKKKVTSIDKANVLESSKLWRIVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Sequences:

>Translated_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSCAFQLASKRKKKVTSIDKANVLESSKLWRIVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
>Mature_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSCAFQLASKRKKKVTSIDKANVLESSKLWRIVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=30.945558739255, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=31.0541310541311, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=31.304347826087, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI4758582, Length=255, Percent_Identity=30.9803921568627, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=34.5360824742268, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=30.8695652173913, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=51.0510510510511, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.1123595505618, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=23.8605898123324, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.638418079096, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.3977272727273, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=27.683615819209, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=27.3775216138329, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=47.7653631284916, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.6946778711485, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=346, Percent_Identity=33.2369942196532, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=29.4617563739377, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=31.8965517241379, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=31.8965517241379, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=29.8295454545455, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=29.8295454545455, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=27.4509803921569, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=26.7241379310345, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=26.7241379310345, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=26.7241379310345, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=26.7241379310345, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=26.9565217391304, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=24.2690058479532, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_BACAN (Q81T67)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843878.1
- RefSeq:   YP_018042.2
- RefSeq:   YP_027581.1
- ProteinModelPortal:   Q81T67
- SMR:   Q81T67
- EnsemblBacteria:   EBBACT00000010614
- EnsemblBacteria:   EBBACT00000016099
- EnsemblBacteria:   EBBACT00000019482
- GeneID:   1084163
- GeneID:   2816381
- GeneID:   2848026
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_1421
- KEGG:   bar:GBAA_1421
- KEGG:   bat:BAS1312
- TIGR:   BA_1421
- TIGR:   GBAA_1421
- GeneTree:   EBGT00050000000661
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   BANT260799:BAS1312-MONOMER
- BioCyc:   BANT261594:GBAA1421-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 38329; Mature: 38329

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 94-94 BINDING 104-104 BINDING 130-130 BINDING 215-215

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKK
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEEEECCHHHHHHHHHH
ADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEIERIVSCAFQLASKRKKKVTS
CCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRIVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKK
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEEEECCHHHHHHHHHH
ADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEIERIVSCAFQLASKRKKKVTS
CCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRIVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629