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The map label for this gene is prpD [H]

Identifier: 227814840

GI number: 227814840

Start: 2054071

End: 2055507

Strand: Reverse

Name: prpD [H]

Synonym: BAMEG_2251

Alternate gene names: 227814840

Gene position: 2055507-2054071 (Counterclockwise)

Preceding gene: 227814841

Following gene: 227814839

Centisome position: 39.3

GC content: 36.26

Gene sequence:

>1437_bases
ATGATTAAAACAAATGAAATTAAACAAAAAGATGCAATATTAGAAGAGATTACGGATTATGTATTAAATAAAGAGGTAAC
AAGTGCAGAAGCATTCAGTACTGCTCGTTACGTATTATTTGATACACTTGGATGCGGAATTTTAGCATTACAATATCCAG
AGTGTACGAAATTATTAGGACCAGTTGTACCAGGAACAATCGTGCCAAATGGAACACGAGTGCCAGGTACGTCTTATGTA
TTAGATCCAGTGAAAGGTGCATTTAATATCGGATGTATGATCCGTTGGTTAGACTATAACGATACTTGGCTTGCAGCAGA
ATGGGGACATCCATCTGATAACCTTGGCGGCATTTTAGCAGTTGCAGATTATATTAGCCGTGTTCGTATATCAGAAGGAA
AAGAACCGTTAAAAGTACGTGAAGTATTAGAAATGATGATTAAAGCACATGAAATTCAAGGTGTATTAGCTTTAGAAAAC
AGCTTAAACCGGGTTGGTCTTGACCACGTATTATACGTAAAAGTAGCAACAACTGCTGTAGTTGCGAAAATGCTTGGCGG
AACACGTGAAGAAATCTTTAATGCATTATCACATGCATGGATTGATAATTCTAGTCTTCGTACATATCGTCACGCTCCAA
ATACTGGATCACGTAAATCATGGGCAGCAGGTGATGCAACAAGTCGCGGTGTTCACCTTGCAATGACTGCTTTAAAAGGT
GAAATGGGTTATCCAACAGCATTATCTGCACCGGGTTGGGGATTCCAAGATGTATTATTTAATAAACAAGAATTAAAGTT
AGCTAGACCATTAGAGTCTTATGTAATGGAAAATGTATTATTTAAAGTTTCATATCCAGCAGAATTCCATGCACAAACAG
CTGCAGAATGTGCTGTAAAATTACATCCGGAAATTAAAGAAAGATTAGATGAAATTGACCGTATTACAATTACAACTCAT
GAATCAGCAATTCGTATTATTGATAAAGAAGGTCCATTAAATAACCCAGCTGATCGTGATCATTGTTTACAATATATTAC
GGCAATTGGTTTATTAAAGGGAGATATCGTTGCGGATGATTATGAGGATGCAGTAGCAAATGATCCACGTGTAGATGAAT
TACGTAATAAGATGGTTGTTGTTGAAAACAAACAGTACAGTTTAGATTACCTTGACCCGAACAAGCGCTCAATCGCCAAC
GCTGTTCAAGTTCATTTCAAGGATGGAACTGTAACAGAAAACGTGGAATGTGAATATCCACTTGGTCACCGTTTCCGTAG
AGACGAAGCAATTCCAAAAGTTGTTCAAAAATTCACTGCAAATATGGCAGGTCATTATTCTAGTAAACAGCAAGAACAAA
TTCATGAAGTTTGTTTAAATGAAGAGAAACTAGAAAATATGAATGTAAACGAATTTGTAGATCTATTCTTAATTTAA

Upstream 100 bases:

>100_bases
AAAAACAACTGGGGAGTTGTTAGCACCGCCCGCCAGATGGGTGTTTGACCGACACCCATCATTAATAATAACGAATTTTC
GACAGAAAGGGAAGGATAGC

Downstream 100 bases:

>100_bases
TAATAGGGGGAATTTAGAATGGCTTGGGTTGTGAATAAACAGTCAACACAAGAGGAGCTTGCGAATCGCTTCCGAGCTTT
AGTAGAAGCAAATGAAATTT

Product: 2-methylcitrate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLFDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYV
LDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEPLKVREVLEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
EMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTH
ESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIAN
AVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI

Sequences:

>Translated_478_residues
MIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLFDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYV
LDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEPLKVREVLEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
EMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTH
ESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIAN
AVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI
>Mature_478_residues
MIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLFDTLGCGILALQYPECTKLLGPVVPGTIVPNGTRVPGTSYV
LDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAVADYISRVRISEGKEPLKVREVLEMMIKAHEIQGVLALEN
SLNRVGLDHVLYVKVATTAVVAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
EMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQTAAECAVKLHPEIKERLDEIDRITITTH
ESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADDYEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIAN
AVQVHFKDGTVTENVECEYPLGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI

Specific function: Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate [H]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Escherichia coli, GI1786528, Length=466, Percent_Identity=62.6609442060086, Blast_Score=629, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6325258, Length=486, Percent_Identity=52.6748971193416, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012705
- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: =4.2.1.79 [H]

Molecular weight: Translated: 53458; Mature: 53458

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLFDTLGCGILALQYPECTKLLG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHC
PVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHH
VADYISRVRISEGKEPLKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCCEEEEHHHHHHH
VAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCC
EMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQTAAECAVK
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHC
LHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADD
CCHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
YEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYP
CHHHHCCCCCHHHHCCCEEEEECCCEEEECCCCCHHHHHHEEEEEECCCCCCCCCEECCC
LGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MIKTNEIKQKDAILEEITDYVLNKEVTSAEAFSTARYVLFDTLGCGILALQYPECTKLLG
CCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHC
PVVPGTIVPNGTRVPGTSYVLDPVKGAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILA
CCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEEEECCCCEEEEECCCCCCCCCHHHH
VADYISRVRISEGKEPLKVREVLEMMIKAHEIQGVLALENSLNRVGLDHVLYVKVATTAV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCCEEEEHHHHHHH
VAKMLGGTREEIFNALSHAWIDNSSLRTYRHAPNTGSRKSWAAGDATSRGVHLAMTALKG
HHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCC
EMGYPTALSAPGWGFQDVLFNKQELKLARPLESYVMENVLFKVSYPAEFHAQTAAECAVK
CCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHC
LHPEIKERLDEIDRITITTHESAIRIIDKEGPLNNPADRDHCLQYITAIGLLKGDIVADD
CCHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEECCC
YEDAVANDPRVDELRNKMVVVENKQYSLDYLDPNKRSIANAVQVHFKDGTVTENVECEYP
CHHHHCCCCCHHHHCCCEEEEECCCEEEECCCCCHHHHHHEEEEEECCCCCCCCCEECCC
LGHRFRRDEAIPKVVQKFTANMAGHYSSKQQEQIHEVCLNEEKLENMNVNEFVDLFLI
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8759838 [H]