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The map label for this gene is mmgD [H]

Identifier: 227814841

GI number: 227814841

Start: 2055606

End: 2056727

Strand: Reverse

Name: mmgD [H]

Synonym: BAMEG_2252

Alternate gene names: 227814841

Gene position: 2056727-2055606 (Counterclockwise)

Preceding gene: 227814842

Following gene: 227814840

Centisome position: 39.32

GC content: 36.54

Gene sequence:

>1122_bases
ATGATGAAAGCTGAAGAAAAATTTTCCCCGGGATTAGATGGTATAGTGGCAGCGGAGACGAAGATCTCGTTTCTTGACAC
AGTGAAAGGTGAAATTGTAATTCAAGGGTATGATTTAATCGAACTCTCAAAAACAAAAGAGTATTTAGACATTGTGCACC
TTTTATTGGAAGAACATCTACCGAATGAGGATGAAAAAGCAACACTTGAAAAGAAATTAAAAGAAGAATACGCGGTACCA
GAAGGTGTATTCAACGTATTAAAGGCATTACCGAAAGAAACGCACCCTATGGATGGATTGCGTACAGGTGTGTCCGCATT
AGCTGGTTACGATAATGATATTGAAAACCGCTCGTTAGAAGTAAACAAAAGCCGCGGATACAAATTATTGAGTAAAGTAC
CAAACATTGTAGCGAATAGCTATCATATTTTAAATAATGAAGAGCCAATCGAACCACTTAAGGAATTATCATATAGTGCA
AATTTCTTCTATATGTTAACAGGTAAAAAACCAACTGAACTTGAGGAGAAGATCTTCGATCGTTCCCTTGTTTTATATAG
TGAACATGAAATGCCAAACTCTACATTTACAGCGCGCGTTATTGCATCTACACAATCCGACTTATATGGTGCTTTAACAG
GTGCGGTTGCATCTTTAAAAGGTAGCTTACACGGCGGTGCAAACGAAGCGGTTATGTACATGCTTTTAGAAGCTGGTAAT
GTTGAGAAATTTGAAGAATTATTACAGAAGAAACTATATAACAAAGAAAAAATTATGGGCTTTGGACACCGTGTATATAT
GAAGAAAATGGATCCAAGAGCTTTAATGATGAAGGAAGCTTTAAAACAGTTATGTGATGTAAAAGGTGATTATACACTAT
ATGAAATGTGTGAAGCTGGAGAAAAGATTATGGAGAAGGAAAAAGGAATTTATCCAAACCTTGATTATTATGCAGCTCCA
GTATATTGGATGCTTGGTATTCCAATTCAACTATACACGCCAATCTTCTTTAGCTCTAGAACAGTAGGACTATGTGCGCA
CGTTATTGAACAACATACGAACAATCGTTTGTTCCGTCCACGTGTAAATTATATTGGCGAGCGACATGTACTTAGTAAGT
AA

Upstream 100 bases:

>100_bases
CTTCTAAAAACTCTGATACATTGAAATAGAAATGTAGTTTCACAATATTGTAAAGGCTTACAGTTATGTGCACGAAGATC
TATTAGGATTGGAGGAAATA

Downstream 100 bases:

>100_bases
AAACAACTGGGGAGTTGTTAGCACCGCCCGCCAGATGGGTGTTTGACCGACACCCATCATTAATAATAACGAATTTTCGA
CAGAAAGGGAAGGATAGCAT

Product: citrate synthase 3

Products: NA

Alternate protein names: Citrate synthase 3; Citrate synthase III; Methylcitrate synthase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MMKAEEKFSPGLDGIVAAETKISFLDTVKGEIVIQGYDLIELSKTKEYLDIVHLLLEEHLPNEDEKATLEKKLKEEYAVP
EGVFNVLKALPKETHPMDGLRTGVSALAGYDNDIENRSLEVNKSRGYKLLSKVPNIVANSYHILNNEEPIEPLKELSYSA
NFFYMLTGKKPTELEEKIFDRSLVLYSEHEMPNSTFTARVIASTQSDLYGALTGAVASLKGSLHGGANEAVMYMLLEAGN
VEKFEELLQKKLYNKEKIMGFGHRVYMKKMDPRALMMKEALKQLCDVKGDYTLYEMCEAGEKIMEKEKGIYPNLDYYAAP
VYWMLGIPIQLYTPIFFSSRTVGLCAHVIEQHTNNRLFRPRVNYIGERHVLSK

Sequences:

>Translated_373_residues
MMKAEEKFSPGLDGIVAAETKISFLDTVKGEIVIQGYDLIELSKTKEYLDIVHLLLEEHLPNEDEKATLEKKLKEEYAVP
EGVFNVLKALPKETHPMDGLRTGVSALAGYDNDIENRSLEVNKSRGYKLLSKVPNIVANSYHILNNEEPIEPLKELSYSA
NFFYMLTGKKPTELEEKIFDRSLVLYSEHEMPNSTFTARVIASTQSDLYGALTGAVASLKGSLHGGANEAVMYMLLEAGN
VEKFEELLQKKLYNKEKIMGFGHRVYMKKMDPRALMMKEALKQLCDVKGDYTLYEMCEAGEKIMEKEKGIYPNLDYYAAP
VYWMLGIPIQLYTPIFFSSRTVGLCAHVIEQHTNNRLFRPRVNYIGERHVLSK
>Mature_373_residues
MMKAEEKFSPGLDGIVAAETKISFLDTVKGEIVIQGYDLIELSKTKEYLDIVHLLLEEHLPNEDEKATLEKKLKEEYAVP
EGVFNVLKALPKETHPMDGLRTGVSALAGYDNDIENRSLEVNKSRGYKLLSKVPNIVANSYHILNNEEPIEPLKELSYSA
NFFYMLTGKKPTELEEKIFDRSLVLYSEHEMPNSTFTARVIASTQSDLYGALTGAVASLKGSLHGGANEAVMYMLLEAGN
VEKFEELLQKKLYNKEKIMGFGHRVYMKKMDPRALMMKEALKQLCDVKGDYTLYEMCEAGEKIMEKEKGIYPNLDYYAAP
VYWMLGIPIQLYTPIFFSSRTVGLCAHVIEQHTNNRLFRPRVNYIGERHVLSK

Specific function: Catalyzes the synthesis of citrate from acetyl-CoA and oxaloacetate. The genomic context suggests that this protein probably functions as a 2-methylcitrate synthase, i.e. catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=373, Percent_Identity=28.4182305630027, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1786527, Length=361, Percent_Identity=38.5041551246537, Blast_Score=257, Evalue=8e-70,
Organism=Escherichia coli, GI1786939, Length=381, Percent_Identity=33.0708661417323, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17555174, Length=373, Percent_Identity=28.9544235924933, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=334, Percent_Identity=25.748502994012, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319850, Length=334, Percent_Identity=25.1497005988024, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325257, Length=344, Percent_Identity=24.7093023255814, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24640126, Length=373, Percent_Identity=28.4182305630027, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24640124, Length=373, Percent_Identity=28.4182305630027, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI21356863, Length=323, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5; =2.3.3.1 [H]

Molecular weight: Translated: 42388; Mature: 42388

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKAEEKFSPGLDGIVAAETKISFLDTVKGEIVIQGYDLIELSKTKEYLDIVHLLLEEHL
CCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHC
PNEDEKATLEKKLKEEYAVPEGVFNVLKALPKETHPMDGLRTGVSALAGYDNDIENRSLE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEE
VNKSRGYKLLSKVPNIVANSYHILNNEEPIEPLKELSYSANFFYMLTGKKPTELEEKIFD
ECHHCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHC
RSLVLYSEHEMPNSTFTARVIASTQSDLYGALTGAVASLKGSLHGGANEAVMYMLLEAGN
CCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
VEKFEELLQKKLYNKEKIMGFGHRVYMKKMDPRALMMKEALKQLCDVKGDYTLYEMCEAG
HHHHHHHHHHHHCCHHHHHCCCHHEEHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EKIMEKEKGIYPNLDYYAAPVYWMLGIPIQLYTPIFFSSRTVGLCAHVIEQHTNNRLFRP
HHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHCHHHCCCCHHHHHHHHHHHHCCCCEECC
RVNYIGERHVLSK
HHHHCCCHHCCCC
>Mature Secondary Structure
MMKAEEKFSPGLDGIVAAETKISFLDTVKGEIVIQGYDLIELSKTKEYLDIVHLLLEEHL
CCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHC
PNEDEKATLEKKLKEEYAVPEGVFNVLKALPKETHPMDGLRTGVSALAGYDNDIENRSLE
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEE
VNKSRGYKLLSKVPNIVANSYHILNNEEPIEPLKELSYSANFFYMLTGKKPTELEEKIFD
ECHHCCHHHHHHHHHHHHCCEEECCCCCCHHHHHHCCCCCCEEEEEECCCCCHHHHHHHC
RSLVLYSEHEMPNSTFTARVIASTQSDLYGALTGAVASLKGSLHGGANEAVMYMLLEAGN
CCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
VEKFEELLQKKLYNKEKIMGFGHRVYMKKMDPRALMMKEALKQLCDVKGDYTLYEMCEAG
HHHHHHHHHHHHCCHHHHHCCCHHEEHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
EKIMEKEKGIYPNLDYYAAPVYWMLGIPIQLYTPIFFSSRTVGLCAHVIEQHTNNRLFRP
HHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHCHHHCCCCHHHHHHHHHHHHCCCCEECC
RVNYIGERHVLSK
HHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377 [H]