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The map label for this gene is nprT [H]

Identifier: 227814620

GI number: 227814620

Start: 1847610

End: 1849034

Strand: Reverse

Name: nprT [H]

Synonym: BAMEG_2030

Alternate gene names: 227814620

Gene position: 1849034-1847610 (Counterclockwise)

Preceding gene: 227814621

Following gene: 227814619

Centisome position: 35.35

GC content: 35.16

Gene sequence:

>1425_bases
ATGGTTAGAGCGTTTGACATAGCGGGCCATGAATTTACACATGCGGTTACATCTAGCGAATCTAATCTTGAATTCTTTGG
GGAGTCTGGGGCAATTAACGAGGCATTATCTGATATTATGGGAACAGCTATTGAAAAATATATAAATAATGGGAAATTTA
ATTGGACAATAGGAGAACAAAGCGGATCCGTTCTCCGTGATATGAAAAATCCATCTTCAGTCAAATTTTTTGATGGAGTA
CCGTACCCAGATGATTATAGTAAATACAGTGATTTAAACGGAGAAGATAATGAGGGCGTTCATTTTAACTCAAGTATTAT
TAATAAAGTCGCGTATTTAATTGCACAAGGCGGCACTCATAACGGTGTAACTGTAAATGGTATTGGTGAAGATAAAATGT
TTGATATTTTCTATTATGCAAATACTGATGAATTGAACATGACTTCTAATTTCTCTGAATTAAGATTAGCGTGTCTTAAA
GTTGCAACAAATAAATATGGAGCAAATTCAATTGAGGTTGAAACGGTCCAAAAAGCTTTTGATGCAGCAAAAATTAAAGG
ACCAGTGAAAGAAGACGAAAAAACTGAGGTAAACCAAGTCCCAGAGTTAACTGTACCACTTACAATTACACTACGTGTAG
GTGATACGTTTGACCCGATAAGCAATGTAAAAGCTATTGATAAAGAAGATGGTGATTTAACAACTCGAGTAAAACATAAA
GGTGATGTAGATACTTCTAAACCAGGCAAATACATTGTGGATTACTCTGTCATTGATTCTCAAGGCGGGAATGCAACGGC
AACACAGACTGTAATTGTAGAGGGAAATGGAGAAACAGCAGATCTAAAACCTACATTAACAGTTCCTGTTGCAACGACAA
TTACTGTAGGAGATTCATTCGATCCAATGGCAAAAGTAAAAGCTATTGATAAAGAAGACAGCAATCTTACTTCTAAAGTA
AAAATTGATGGTGAAGTAGATACCTCTAAAGCCGGTACATATGTATTAACGTACAAAGTTACAGATTCAAAAGGTAATGA
AGGAACTGCGAAACAAACTGTAACGGTTAAAGTTAGAGAAGAAGTTAAGAATGAAATACCAATACTACAAGTACCTGCTA
CTACTACAATTACTAAGGGAGATAAATTTGATCTAATGGTAGGAGTATCAGCTACTGATAAAGAGGACGGTGATCTTACT
TCTAAAGTCATGTATGAAGGAACTGTAGATACCTCTAGAACTGGTACTTTTGAAATAAAATATAGTGTCAGAGATTCTGT
AGGTAATGAGGTACATACAATACAAGAAGTATTTGTGAAAGATAAAGACACTGGAAAAACTAATAGCCTTGCTAACAACA
GTAATTCTAATAAAAATGAATCTACATATAAAGAGCTTCCCAATACGGGGGGGCAGTACAACTAA

Upstream 100 bases:

>100_bases
ACATGCATGGCATTCAGGAGAAACAGATGACCCTAAAAATTGGGGGAATGCATTTAGTGCTAATATTAACAATGTGAGTA
TGCTTATATATGGTGATCCA

Downstream 100 bases:

>100_bases
CAGTACAACAATGGGATTATGGATGGTTATTGTAGGTACAGTACTCACTTTGGCTCGTAAATTTAGAAAGATACAAAAAT
AATAAGGATTATTCGTTTTA

Product: thermolysin metallopeptidase, alpha-helical domain protein

Products: NA

Alternate protein names: Thermostable neutral proteinase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MVRAFDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSVLRDMKNPSSVKFFDGV
PYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTHNGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLK
VATNKYGANSIEVETVQKAFDAAKIKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHK
GDVDTSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMAKVKAIDKEDSNLTSKV
KIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVREEVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLT
SKVMYEGTVDTSRTGTFEIKYSVRDSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN

Sequences:

>Translated_474_residues
MVRAFDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSVLRDMKNPSSVKFFDGV
PYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTHNGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLK
VATNKYGANSIEVETVQKAFDAAKIKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHK
GDVDTSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMAKVKAIDKEDSNLTSKV
KIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVREEVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLT
SKVMYEGTVDTSRTGTFEIKYSVRDSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN
>Mature_474_residues
MVRAFDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQSGSVLRDMKNPSSVKFFDGV
PYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTHNGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLK
VATNKYGANSIEVETVQKAFDAAKIKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHK
GDVDTSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSFDPMAKVKAIDKEDSNLTSKV
KIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVREEVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLT
SKVMYEGTVDTSRTGTFEIKYSVRDSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN

Specific function: Extracellular zinc metalloprotease [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C [H]

EC number: =3.4.24.27 [H]

Molecular weight: Translated: 51475; Mature: 51475

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00142 ZINC_PROTEASE ; PS50268 CADHERIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRAFDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQ
CEEEEECCCCCEEHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCC
SGSVLRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTH
CCHHHHHCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHCCCCCC
NGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAF
CCEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
DAAKIKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHK
HHHHCCCCCCCCCCCHHHCCCCEEEEEEEEEEECCCCCCHHCCEECCCCCCCEEEEEECC
GDVDTSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSF
CCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEEEEEECCCC
DPMAKVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVRE
CCHHHHEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEEEHH
EVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIK
HHHHCCCEEEECCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEE
YSVRDSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MVRAFDIAGHEFTHAVTSSESNLEFFGESGAINEALSDIMGTAIEKYINNGKFNWTIGEQ
CEEEEECCCCCEEHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCC
SGSVLRDMKNPSSVKFFDGVPYPDDYSKYSDLNGEDNEGVHFNSSIINKVAYLIAQGGTH
CCHHHHHCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCEEECHHHHHHHHHHHCCCCCC
NGVTVNGIGEDKMFDIFYYANTDELNMTSNFSELRLACLKVATNKYGANSIEVETVQKAF
CCEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHH
DAAKIKGPVKEDEKTEVNQVPELTVPLTITLRVGDTFDPISNVKAIDKEDGDLTTRVKHK
HHHHCCCCCCCCCCCHHHCCCCEEEEEEEEEEECCCCCCHHCCEECCCCCCCEEEEEECC
GDVDTSKPGKYIVDYSVIDSQGGNATATQTVIVEGNGETADLKPTLTVPVATTITVGDSF
CCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCCCCCCCCEEEEEEEEEEEECCCC
DPMAKVKAIDKEDSNLTSKVKIDGEVDTSKAGTYVLTYKVTDSKGNEGTAKQTVTVKVRE
CCHHHHEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEEEEEHH
EVKNEIPILQVPATTTITKGDKFDLMVGVSATDKEDGDLTSKVMYEGTVDTSRTGTFEIK
HHHHCCCEEEECCCEEEECCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCEEEEE
YSVRDSVGNEVHTIQEVFVKDKDTGKTNSLANNSNSNKNESTYKELPNTGGQYN
EEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2993245 [H]