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The map label for this gene is ddl

Identifier: 227814575

GI number: 227814575

Start: 1805455

End: 1806369

Strand: Reverse

Name: ddl

Synonym: BAMEG_1985

Alternate gene names: 227814575

Gene position: 1806369-1805455 (Counterclockwise)

Preceding gene: 227814576

Following gene: 227814574

Centisome position: 34.54

GC content: 34.54

Gene sequence:

>915_bases
ATGAGAATTGGCGTTATTATGGGAGGAGTATCCTCTGAAAAACAAGTATCAATTATGACTGGAAACGAAATGATTGCAAA
TTTAGATAAAAATAAATATGAAATCGTACCAATTACGTTAAATGAAAAAATGGATTTAATCGAAAAAGCGAAAGACATTG
ATTTTGCGCTGCTCGCATTACACGGAAAATACGGAGAAGATGGGACAGTTCAAGGAACGCTTGAAAGTTTAGGTATTCCT
TATAGCGGCAGCAATATGTTATCTAGCGGTATATGTATGGACAAAAATATTTCGAAAAAGATTTTACGTTATGAAGGAAT
AGAAACGCCAGATTGGATTGAACTTACAAAAATGGAGGATTTGAATTTTGATGAGTTAGATAAGTTAGGATTTCCATTAG
TGGTAAAACCAAACTCTGGTGGTTCAAGTGTTGGGGTAAAGATTGTCTATGATAAAGACGAATTAATTTCAATGCTGGAA
ACTGTGTTCGAATGGGATTCTGAAGTAGTAATAGAGAAATATATAAAAGGTGAAGAGATTACATGTTCCATTTTTGATGG
GAAACAGTTACCTATCATTTCAATTCGGCATGCGGCTGAGTTCTTTGATTACAATGCGAAATATGATGACGCTAGTACAA
TTGAAGAAGTTATCGAACTTCCAGCTGAACTTAAGGAACGTGTAAACAAAGCATCACTGGCGTGCTATAAAGCATTAAAA
TGTAGTGTTTATGCAAGGGTTGATATGATGGTGAAGGACGGTATTCCATATGTAATGGAGGTTAATACATTACCAGGGAT
GACACAAGCAAGTTTACTACCAAAAAGTGCAGATGCAGCGGGAATTCATTATAGTAAACTATTAGATATGATTATTGAAA
CTTCATTAAGAGTAAGAAAAGAAGAAGGGTTTTAA

Upstream 100 bases:

>100_bases
ACATTTCGATTAGGATTTTCACGTCTGAAAGAAGAAGAAATAGTTCGGGGAATCAAAATAATCGGTGATACAATAAAAAA
TGAAATTTGGAGTTGAGGAA

Downstream 100 bases:

>100_bases
GTAACGGTATTATGAATACTTTTGACAGTAACAAGAAGTATGTTATGATGAATACGATACGAAAATAAAATATTTATCCA
CTAGGGGGGCCTATTATAGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLE
TVFEWDSEVVIEKYIKGEEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF

Sequences:

>Translated_304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLE
TVFEWDSEVVIEKYIKGEEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF
>Mature_304_residues
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIP
YSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLE
TVFEWDSEVVIEKYIKGEEITCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRKEEGF

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=301, Percent_Identity=36.5448504983389, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=29.512893982808, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BACAA (C3PB71)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002866914.1
- ProteinModelPortal:   C3PB71
- SMR:   C3PB71
- EnsemblBacteria:   EBBACT00000127698
- GeneID:   7853365
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_2673
- GeneTree:   EBGT00050000001406
- ProtClustDB:   PRK01372
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33853; Mature: 33853

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLAL
CEEEEEECCCCCCCEEEEEECCHHEECCCCCCEEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMED
ECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
LNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLETVFEWDSEVVIEKYIKGEEI
CCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCEE
TCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
EEEEECCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRK
HEEEHEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGF
CCCC
>Mature Secondary Structure
MRIGVIMGGVSSEKQVSIMTGNEMIANLDKNKYEIVPITLNEKMDLIEKAKDIDFALLAL
CEEEEEECCCCCCCEEEEEECCHHEECCCCCCEEEEEEEECCHHHHHHHHCCCCEEEEEE
HGKYGEDGTVQGTLESLGIPYSGSNMLSSGICMDKNISKKILRYEGIETPDWIELTKMED
ECCCCCCCCEEEEHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCC
LNFDELDKLGFPLVVKPNSGGSSVGVKIVYDKDELISMLETVFEWDSEVVIEKYIKGEEI
CCHHHHHHCCCCEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHCCCHHHHHHHCCCCEE
TCSIFDGKQLPIISIRHAAEFFDYNAKYDDASTIEEVIELPAELKERVNKASLACYKALK
EEEEECCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
CSVYARVDMMVKDGIPYVMEVNTLPGMTQASLLPKSADAAGIHYSKLLDMIIETSLRVRK
HEEEHEEEEHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EEGF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA