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The map label for this gene is tenA [H]

Identifier: 227814574

GI number: 227814574

Start: 1804464

End: 1805180

Strand: Reverse

Name: tenA [H]

Synonym: BAMEG_1983

Alternate gene names: 227814574

Gene position: 1805180-1804464 (Counterclockwise)

Preceding gene: 227814575

Following gene: 227814573

Centisome position: 34.52

GC content: 35.7

Gene sequence:

>717_bases
ATGAAATGGATTATTGGAGGGAATCAAATGACTTTTTCACAATCATTACGTAAAGAAGTAGATTCAATTTGGGAAGCTAG
TTTTCATCATCCTTTTGTAAAAAAACTCGGTGAAGGAACGTTAGATTTAGCTAATTTTCGCTATTACGTGCTTCAAGATT
CCTATTATTTGAGTCACTTTGCTAGAGTACAAACATTAGGAGCTGCAAAAGCGCTTGAATTAGAAACGACTGCTCGCATG
GCACATCATGCTCAAAATACATATGAGGCGGAATTATCTTTACATGAGAACTTTGCGAAGAAACTAGGGATTACACAAGA
AGAAAAAGATAATTTTATCCCTGCTCCAACTGCATATGCATATACTTCACATATGTACCGTGCTGCGTATGAAGGACATT
TAGGCGATATTATTGCAGCAATTTTACCTTGCTATTGGCTGTATTATGAAATTGGTGAGCGTTTAAAAGAGTGTCAGCCA
GAAGAGCCGATTTATAACGAATGGATTTCTGCTTATGGATCTGATTGGTTCCGAACATTAGTTGAAGAGCAAATTACACG
ATTAGATGCAATAGCTGAAAAAGTGACAGAGTCTGATCGTAAGCGTATGAAACAGCATTTTGTTATAAGTAGTCAATATG
AATATTCATTTTGGGAAATGGCTTATACTTTAGAAAAGTGGCCGGTAGATACAGAAGTAAAAGATGTAATAAGATAA

Upstream 100 bases:

>100_bases
TTGCTATTTCATGTATAAATACTGTCAATTTCACTTTCTTTACGCCTGTAAAGAAAGTTTTTTTATTATAAAGCGTTAAT
TGCTGTGGATAAATACTTAT

Downstream 100 bases:

>100_bases
AGAAAAGAGGTATTGCTATACGTATGTATGGACAATACCTCTTTTTAGGTTTTTATAAAATCATTGTAAAAATCACAAAT
AAAACACTCCTTTATATTGT

Product: transcriptional activator TenA

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALELETTARM
AHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQP
EEPIYNEWISAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR

Sequences:

>Translated_238_residues
MKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALELETTARM
AHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQP
EEPIYNEWISAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
>Mature_238_residues
MKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHFARVQTLGAAKALELETTARM
AHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQP
EEPIYNEWISAYGSDWFRTLVEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=202, Percent_Identity=26.7326732673267, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325378, Length=205, Percent_Identity=25.8536585365854, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324997, Length=201, Percent_Identity=21.8905472636816, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 27940; Mature: 27940

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHF
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEHHHCEEEEEECHHHHHHH
ARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
YTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYGSDWFRTL
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHH
VEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure
MKWIIGGNQMTFSQSLRKEVDSIWEASFHHPFVKKLGEGTLDLANFRYYVLQDSYYLSHF
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEHHHCEEEEEECHHHHHHH
ARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITQEEKDNFIPAPTAYA
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
YTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGERLKECQPEEPIYNEWISAYGSDWFRTL
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHH
VEEQITRLDAIAEKVTESDRKRMKQHFVISSQYEYSFWEMAYTLEKWPVDTEVKDVIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]