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The map label for this gene is acoL [H]

Identifier: 227814415

GI number: 227814415

Start: 1658242

End: 1659621

Strand: Direct

Name: acoL [H]

Synonym: BAMEG_1823

Alternate gene names: 227814415

Gene position: 1658242-1659621 (Clockwise)

Preceding gene: 227814414

Following gene: 227814417

Centisome position: 31.71

GC content: 37.97

Gene sequence:

>1380_bases
ATGAGTAAATTAGTCGTTATTGGAGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCGGCTCAAAATGGAAAAAATGT
CACTCTTATTGATGAAGCTGATCTTGGCGGGACTTGTTTAAATGTCGGGTGCATGCCTACAAAATCATTGTTAGAAAGTG
CAGAAGTGCATGACATTGTGAGAAAATCGAATCATTATGGAGTTACGCTTAACAATGGAAGTATATCAATCGATTGGAAG
CAAATGCAGGTGAGAAAATCGCAAATCGTAACGCAGCTCGTCCAAGGAATTCAATATTTAATGAAGAAAAATAAAATAAA
AGTTATACAAGGTAAAGCGAAATTTGAAACGGATCATCGTGTGAGAGTTACATACGGTGATAAAGAAATTGTAGTAGATG
GAGAACAGTTCATTATAGCGACAGGTTCAGAACCGACTGAATTACCTTTTGCTCCATTTGATGGGAAGTGGATTTTAAAT
AGTACCCATGCAATGTCTCTAAAAAACATACCGAAATCATTATTAATCGTTGGCGGTGGTGTAATAGGGTGCGAGTTTGC
AAGTATTTATAGTCGTCTTGGAACAAAAGTTACAATTGTTGAAATGGCACCGCAATTATTACCAGGTGAAGATGAAGATA
TCGCACACATTTTAAGAGGGAAACTAGAAAATGATGGCGTGAAAATATTTACAGGAGCAACTTTAAAAGGATTAAATAGT
TATAAGAAGCAGGCTTTATTTGAATATGAAGGAGGTATCCAAGAAGTTAATCCAGAATTTGTTCTCGTTTCTGTCGGCCG
AAAACCACGTGTACAACAATTAAACTTAGAAAAAGCAGGCATTCAATATTCAAATAAAGGAATTTCTGTGAATGAACATA
TGCAAACCAATGTATCACATATTTACGCAGCAGGTGATGTGATTGGTGGAATCCAGCTTGCTCACGTTGCATTCCATGAA
GGAACGACGGCAGCATTATACGCGAGTGGAGAAGATGTAAAAGTAAACTACCATGCTGTACCTCGCTGTATTTATACCGC
TCCAGAAATTGCTAGCGTCGGTTTAACAGAAAAAGATGCAAAAGAGCAGTACGGTGACATACAAATTGGCGAATTTCCTT
TTACAGCGAATGGAAAAGCGCTTATTATTGGAGAACAAACGGGGAAAGTAAAAGTTATTGTGGAACCTAAATATCAAGAA
ATTGTTGGAATTTCTATTATTGGTCCTCGCGCAACAGAACTAATTGGTCAAGGAACTGTAATGATTCATACGGAAGTTAC
CGCTGATATTATGAGAGATTATATTGCAGCGCATCCAACTTTATCTGAATCGATTCATGAAGCTTTATTGCAAGCTGTAG
GGCATGCTGTACATGCTTAA

Upstream 100 bases:

>100_bases
TCGTGTACTAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGCTATTTAGAAGAACCTGTAACAATTCTTT
TATAAAGAGAAGGTGAGAGA

Downstream 100 bases:

>100_bases
ATGGAATTATAATAAACCACTATTTTCTAAATGAATAAGAGAATAGTGGTTTGAATTTTTACGGAAGAAATTGAATATCC
TATTATTTTTTTACGTCAAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of acetoin cleaving system [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKSNHYGVTLNNGSISIDWK
QMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHRVRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILN
STHAMSLKNIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVKIFTGATLKGLNS
YKKQALFEYEGGIQEVNPEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHE
GTTAALYASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAKEQYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQE
IVGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSESIHEALLQAVGHAVHA

Sequences:

>Translated_459_residues
MSKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKSNHYGVTLNNGSISIDWK
QMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHRVRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILN
STHAMSLKNIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVKIFTGATLKGLNS
YKKQALFEYEGGIQEVNPEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHE
GTTAALYASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAKEQYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQE
IVGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSESIHEALLQAVGHAVHA
>Mature_458_residues
SKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIVRKSNHYGVTLNNGSISIDWKQ
MQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHRVRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILNS
THAMSLKNIPKSLLIVGGGVIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVKIFTGATLKGLNSY
KKQALFEYEGGIQEVNPEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSHIYAAGDVIGGIQLAHVAFHEG
TTAALYASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDAKEQYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQEI
VGISIIGPRATELIGQGTVMIHTEVTADIMRDYIAAHPTLSESIHEALLQAVGHAVHA

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=35.6521739130435, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI50301238, Length=448, Percent_Identity=28.7946428571429, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI148277065, Length=459, Percent_Identity=27.6688453159041, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI33519430, Length=451, Percent_Identity=27.9379157427938, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI33519428, Length=451, Percent_Identity=27.9379157427938, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI33519426, Length=451, Percent_Identity=27.9379157427938, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI148277071, Length=467, Percent_Identity=27.6231263383298, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=28.3842794759825, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI291045266, Length=458, Percent_Identity=26.6375545851528, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI291045268, Length=449, Percent_Identity=24.7216035634744, Blast_Score=115, Evalue=7e-26,
Organism=Escherichia coli, GI1786307, Length=449, Percent_Identity=33.8530066815145, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=31.7073170731707, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI87082354, Length=456, Percent_Identity=27.4122807017544, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=28.2758620689655, Blast_Score=168, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=37.3390557939914, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=27.3305084745763, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=26.0180995475113, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=26.0180995475113, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=26.1954261954262, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=223, Percent_Identity=29.5964125560538, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=38.3474576271186, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=26.6666666666667, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325166, Length=449, Percent_Identity=26.9487750556793, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=37.2549019607843, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI24640553, Length=468, Percent_Identity=28.2051282051282, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24640549, Length=468, Percent_Identity=28.2051282051282, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24640551, Length=468, Percent_Identity=28.2051282051282, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17737741, Length=467, Percent_Identity=27.8372591006424, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49563; Mature: 49431

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIV
CCEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKSNHYGVTLNNGSISIDWKQMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHR
HCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCE
VRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILNSTHAMSLKNIPKSLLIVGGG
EEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCEEEECCCCEEHHHCCCCEEEEECCC
VIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVKIFTGATLKGLNS
EEEHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHH
YKKQALFEYEGGIQEVNPEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSH
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCHHHCCEECCCCCCCCHHHHCCCCE
IYAAGDVIGGIQLAHVAFHEGTTAALYASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDA
EEECCCHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCCHHHCCCCCHHH
KEQYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQEIVGISIIGPRATELIGQGTV
HHHCCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECCCHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSESIHEALLQAVGHAVHA
EEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLVVIGGGPAGYVAAITAAQNGKNVTLIDEADLGGTCLNVGCMPTKSLLESAEVHDIV
CEEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHH
RKSNHYGVTLNNGSISIDWKQMQVRKSQIVTQLVQGIQYLMKKNKIKVIQGKAKFETDHR
HCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCE
VRVTYGDKEIVVDGEQFIIATGSEPTELPFAPFDGKWILNSTHAMSLKNIPKSLLIVGGG
EEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCEEEECCCCEEHHHCCCCEEEEECCC
VIGCEFASIYSRLGTKVTIVEMAPQLLPGEDEDIAHILRGKLENDGVKIFTGATLKGLNS
EEEHHHHHHHHHHCCEEEEEECCHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHH
YKKQALFEYEGGIQEVNPEFVLVSVGRKPRVQQLNLEKAGIQYSNKGISVNEHMQTNVSH
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCHHHCCEECCCCCCCCHHHHCCCCE
IYAAGDVIGGIQLAHVAFHEGTTAALYASGEDVKVNYHAVPRCIYTAPEIASVGLTEKDA
EEECCCHHCCEEEEEEEEECCCEEEEEECCCEEEEEEEECCEEEECCCCHHHCCCCCHHH
KEQYGDIQIGEFPFTANGKALIIGEQTGKVKVIVEPKYQEIVGISIIGPRATELIGQGTV
HHHCCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCHHHEEEEEEECCCHHHHHCCCEE
MIHTEVTADIMRDYIAAHPTLSESIHEALLQAVGHAVHA
EEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]