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The map label for this gene is acoC [H]

Identifier: 227814414

GI number: 227814414

Start: 1657030

End: 1658226

Strand: Direct

Name: acoC [H]

Synonym: BAMEG_1822

Alternate gene names: 227814414

Gene position: 1657030-1658226 (Clockwise)

Preceding gene: 227814413

Following gene: 227814415

Centisome position: 31.68

GC content: 36.76

Gene sequence:

>1197_bases
ATGGCTGTAGAAGTTGTAATGCCGAAGTTAGGGATGGCGATGAAAGAGGGTATTATTACGAGCTGGAATATTAAAGCGGG
TGATAATGTGGCAAAAGGAGAATTAATTGCCAGTATTAACTCGGAAAAAATTGAAACGGAAATAGAAGCACCAGCTGATG
GAACTATTCTTGATATAGCTGTAAGTGAAGATGAGGGGGTTCCACCTGGCACTGTAATTTGCTACATTGGTAAACCGAAT
GAAAAAGTAGAGGTACATGAAAGCACAAAGGTTGAGGAAGAACTGACAGCGAGTGAAGTACCACAAAATGTACAACATCC
AGAACCGTTAGGGAAAGAAGTTACGAATAAACAAAGAATCAAAATATCTCCTGTAGCGAAGAAGATTGCGAAAACTGAAA
ATCTTGATATTAGAGCATTACTTGGTACAGGTCCAGGTGGAAGAATTACAAAGGTAGATGTATTAAAGGCACTTGAAGAA
AGAGTAGCAATTCCAGAAGTACTTGAAGAAAGTAAAGTACTTCCTGTTACTGGTATGCGGAAAGCAATCGCAAATCGTAT
GCATGCAAGCTTACAAAATAGTGCGCAATTAACATTAACGATGAAAGTAGATGTGACAGATTTAGTTGCTTTACATAAAG
AAATAGCGGAAGTTGTACAAAAACGATACGATAACAAATTAACCATTACGGATTTCGTTTCTCGAGCTGTTGTATTAGCG
CTTGGGGAGCATAAAGAAATGAACAGCGCTTATATAGATGATGCAATTCATCAATTTGAACATGTTCATTTAGGTATGGC
AGTCGCATTAGAAAAAGGATTAGTCGTTCCAGCTATTCGCTTCGCAAATAATCTCTCATTAGTAGAGTTATCTAAAGAGA
TTAAGAATGCGGCACAAAAGGCACGAGCAGGCAATTTAAATAGTGATGATATGCAAGGAACAACATTTACAATTAGTAAT
TTAGGAAGCTTCGGTATTGAATATTTTACACCCGTATTAAACACACCAGAAACTGGTATTTTAGGTGTAGGTGCAATTGA
ACATGTTCCTGTATATAAAGGGAAAAAATTAAAAAAAGGAAGTATGTTACCTTTAAGTTTAACATTCGATCATCGTGTAC
TAGACGGTGCACCAGCAGCTGCATTTTTACGCACAATTAAACGCTATTTAGAAGAACCTGTAACAATTCTTTTATAA

Upstream 100 bases:

>100_bases
TTTACCAACGCCGGAGAAAGTAATTGAAACTGTATCAGAAATGATCGGAGACCAATCTTTATTACATGTGTAATGAAACG
ATGAGAAGGGAGAGAGAAAT

Downstream 100 bases:

>100_bases
AGAGAAGGTGAGAGAATGAGTAAATTAGTCGTTATTGGAGGCGGACCTGCTGGCTATGTAGCGGCAATTACCGCGGCTCA
AAATGGAAAAAATGTCACTC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Acetoin dehydrogenase E2 component; Dihydrolipoamide acetyltransferase component of acetoin cleaving system [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTILDIAVSEDEGVPPGTVICYIGKPN
EKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIRALLGTGPGGRITKVDVLKALEE
RVAIPEVLEESKVLPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVVLA
LGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNAAQKARAGNLNSDDMQGTTFTISN
LGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Sequences:

>Translated_398_residues
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTILDIAVSEDEGVPPGTVICYIGKPN
EKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIRALLGTGPGGRITKVDVLKALEE
RVAIPEVLEESKVLPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVVLA
LGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNAAQKARAGNLNSDDMQGTTFTISN
LGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
>Mature_397_residues
AVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTILDIAVSEDEGVPPGTVICYIGKPNE
KVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRIKISPVAKKIAKTENLDIRALLGTGPGGRITKVDVLKALEER
VAIPEVLEESKVLPVTGMRKAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVVLAL
GEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNAAQKARAGNLNSDDMQGTTFTISNL
GSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKLKKGSMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL

Specific function: The 2-Oxoglutarate Dehydrogenase Complex Catalyzes The Overall Conversion Of 2-Oxoglutarate To Succinyl-CoA And Co(2). It Contains Multiple Copies Of Three Enzymatic Components: 2- Oxoglutarate Dehydrogenase (E1), Dihydrolipoamide Succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=30.3944315545244, Blast_Score=195, Evalue=8e-50,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=26.8065268065268, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI203098753, Length=463, Percent_Identity=25.9179265658747, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI203098816, Length=463, Percent_Identity=25.9179265658747, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI260898739, Length=180, Percent_Identity=31.1111111111111, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=32.1951219512195, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1786305, Length=419, Percent_Identity=28.8782816229117, Blast_Score=154, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=30.2325581395349, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=28.921568627451, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=25.4761904761905, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=301, Percent_Identity=26.2458471760797, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=401, Percent_Identity=30.4239401496259, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=26.457399103139, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24582497, Length=294, Percent_Identity=30.952380952381, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI20129315, Length=294, Percent_Identity=30.952380952381, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=25.6944444444444, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24645909, Length=221, Percent_Identity=34.3891402714932, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43237; Mature: 43105

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTILDIA
CCEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHEEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRI
ECCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEE
KISPVAKKIAKTENLDIRALLGTGPGGRITKVDVLKALEERVAIPEVLEESKVLPVTGMR
EECHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHH
KAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVVLA
HHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
LGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNAAQK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCHHHHHHHHHHHHHHH
ARAGNLNSDDMQGTTFTISNLGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKLKKG
HHCCCCCCCCCCCCEEEECCCCHHCHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCCCCC
SMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
AVEVVMPKLGMAMKEGIITSWNIKAGDNVAKGELIASINSEKIETEIEAPADGTILDIA
CEEEECHHHHHHHHCCCEEEEECCCCCCCCCCCEEEECCCHHHEEEECCCCCCCEEEEE
VSEDEGVPPGTVICYIGKPNEKVEVHESTKVEEELTASEVPQNVQHPEPLGKEVTNKQRI
ECCCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCEE
KISPVAKKIAKTENLDIRALLGTGPGGRITKVDVLKALEERVAIPEVLEESKVLPVTGMR
EECHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHH
KAIANRMHASLQNSAQLTLTMKVDVTDLVALHKEIAEVVQKRYDNKLTITDFVSRAVVLA
HHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
LGEHKEMNSAYIDDAIHQFEHVHLGMAVALEKGLVVPAIRFANNLSLVELSKEIKNAAQK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCHHHHHHHHHHHHHHH
ARAGNLNSDDMQGTTFTISNLGSFGIEYFTPVLNTPETGILGVGAIEHVPVYKGKKLKKG
HHCCCCCCCCCCCCEEEECCCCHHCHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCCCCC
SMLPLSLTFDHRVLDGAPAAAFLRTIKRYLEEPVTILL
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]