Click here to switch to the map view.

The map label for this gene is acoA [H]

Identifier: 227814412

GI number: 227814412

Start: 1654952

End: 1655950

Strand: Direct

Name: acoA [H]

Synonym: BAMEG_1820

Alternate gene names: 227814412

Gene position: 1654952-1655950 (Clockwise)

Preceding gene: 227814411

Following gene: 227814413

Centisome position: 31.64

GC content: 37.84

Gene sequence:

>999_bases
ATGTTAAAAACAACTGAAAGTAAAGAGAATGAAATTACGAAAGAACAAGCTCGTTGGATGTATGAAAAAATGTTAGAGAT
TCGTAAATTTGAAGATAAGGTACATGAATTGTTCGCTCAAGGCGTGCTGCCAGGTTTTGTTCATTTATACGCAGGTGAAG
AAGCGGTAGCAGTTGGTGTATGTGCCCACTTAACGGATAGTGACAGTATTACAAGTACGCATAGAGGACATGGACACTGT
ATCGCAAAAGGTTGTGATTTAAACGGTATGATGGCTGAACTTTTTGGGAAAGCGACAGGGTTATGTAAAGGTAAAGGCGG
CTCCATGCATATTGCCGATTTAGATAAAGGAATGCTTGGTGCAAATGGCATTGTAGGTGGTGGTTTCCCACTTGCTTGCG
GTTCGGCATTAACAGCTAAATATAAAGGAACGAAAGATGTAAGTGTTTGTTTCTTCGGTGACGGAGCAAATAATGAAGGA
ACATTCCATGAAGGGGTTAATTTAGCGGCGATTTGGAAGTTACCTGTTATTTTTATTGCAGAAAATAACGGTTACGGTGA
AGCAACGACATTTGAATATGCTTCAAGTTGCGATTCTATTGCAGATCGTGCGAAAGCTTATAACATTCCAGGTGTTCAAG
TAGATGGAAAGGATCTTCTAGCTGTATATAAAGCTGCTGAAGAAGCGGTAGAACGTGCGCGTAATGGCGATGGTCCAACA
ATAATTGAATGTATGACATATCGTAATTACGGTCATTTCGAAGGTGAAGCACAAACGTATAAAACTTCAGAAGAAAAAGA
AGAACATTTAAATGAAAAAGATGCGATTGTGAATTTCCGTAAGCATTTAATTCATGAAGCTTTATTAACTGAATCTGAGC
TTGTAGATATGGAAAAGGCAGTAGATGAAGCGGTTCAAAAATCAATTGAATTTAGTGAAAATAGTCCATATCCAGAGGAT
GAAGAGTTATTAAAAGATGTATACGTTTCTTACAAATAA

Upstream 100 bases:

>100_bases
TTTATTTTGCAAGAAAAGAGAAAACCTATTAAATCAAGCATTAGAATTTGGCACAGTACTTGCAATATAAAAGATGAACA
AGAAAATAGGAGGGCTGGAC

Downstream 100 bases:

>100_bases
GAGGAGGGTGAAAAATAATGACTAGAACAGTAAGTATGTCAACTGCAATTAATGAAGCGATGAAAATCTCAATGCGTCGT
GATGAAAACGTAATTTTAAT

Product: TPP-dependent acetoin dehydrogenase E1 alpha-subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKTTESKENEITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLTDSDSITSTHRGHGHC
IAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEG
TFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDEAVQKSIEFSENSPYPED
EELLKDVYVSYK

Sequences:

>Translated_332_residues
MLKTTESKENEITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLTDSDSITSTHRGHGHC
IAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEG
TFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDEAVQKSIEFSENSPYPED
EELLKDVYVSYK
>Mature_332_residues
MLKTTESKENEITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGVCAHLTDSDSITSTHRGHGHC
IAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLGANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEG
TFHEGVNLAAIWKLPVIFIAENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKAVDEAVQKSIEFSENSPYPED
EELLKDVYVSYK

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=320, Percent_Identity=37.8125, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI291084742, Length=320, Percent_Identity=37.1875, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4505685, Length=320, Percent_Identity=37.1875, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI291084744, Length=327, Percent_Identity=36.3914373088685, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI291084757, Length=320, Percent_Identity=32.8125, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI11386135, Length=324, Percent_Identity=29.9382716049383, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI258645172, Length=324, Percent_Identity=29.9382716049383, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=38.3647798742138, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=38.3647798742138, Blast_Score=222, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI86563357, Length=325, Percent_Identity=29.5384615384615, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI86563355, Length=311, Percent_Identity=30.5466237942122, Blast_Score=144, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6321026, Length=322, Percent_Identity=36.9565217391304, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI24639744, Length=323, Percent_Identity=39.0092879256966, Blast_Score=217, Evalue=7e-57,
Organism=Drosophila melanogaster, GI28571106, Length=323, Percent_Identity=39.0092879256966, Blast_Score=217, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24639740, Length=323, Percent_Identity=39.0092879256966, Blast_Score=217, Evalue=7e-57,
Organism=Drosophila melanogaster, GI24639748, Length=310, Percent_Identity=39.6774193548387, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=39.2156862745098, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21355903, Length=326, Percent_Identity=26.9938650306748, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36374; Mature: 36374

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTTESKENEITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCEEEEEE
CAHLTDSDSITSTHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLG
EEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCC
ANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEGTFHEGVNLAAIWKLPVIFIA
CCCCCCCCCHHHCCCHHEECCCCCCCEEEEEEECCCCCCCCHHCCCCEEEEEECCEEEEE
ENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKA
EEEEEEECCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDEAVQKSIEFSENSPYPEDEELLKDVYVSYK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLKTTESKENEITKEQARWMYEKMLEIRKFEDKVHELFAQGVLPGFVHLYAGEEAVAVGV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEECCCCEEEEEE
CAHLTDSDSITSTHRGHGHCIAKGCDLNGMMAELFGKATGLCKGKGGSMHIADLDKGMLG
EEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCCEEEEECCCCCCC
ANGIVGGGFPLACGSALTAKYKGTKDVSVCFFGDGANNEGTFHEGVNLAAIWKLPVIFIA
CCCCCCCCCHHHCCCHHEECCCCCCCEEEEEEECCCCCCCCHHCCCCEEEEEECCEEEEE
ENNGYGEATTFEYASSCDSIADRAKAYNIPGVQVDGKDLLAVYKAAEEAVERARNGDGPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCE
IIECMTYRNYGHFEGEAQTYKTSEEKEEHLNEKDAIVNFRKHLIHEALLTESELVDMEKA
EEEEEEECCCCCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDEAVQKSIEFSENSPYPEDEELLKDVYVSYK
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]